흑삼 농축액 첨가 수준에 따른 흑삼 젤리의 품질 특성

김애정 ( Ae Jung Kim ),임희정 ( Hee Jung Lim ),강신정 ( Shin Jung Kang ) 한국식품영양학회 2010 韓國食品營養學會誌 Vol.23 No.2

The principal objective of this study was to evaluate the quality characteristics of black ginseng jelly prepared with different 5 levels(0, 0.5, 1.0, 1.5, and 2.0%) of black ginseng extract. We assessed the ginsenosides level of white and black ginseng for comparison between white and black ginseng. And we conducted the pH, sugar content, Hunter`s color values, the mechanical characteristics and sensory evaluation of black ginseng jelly samples. The levels of ginsenoside Rg3, Rh1, and Rh2 of black ginseng were higher than those of white ginseng. The more black ginseng extract was increased, the sugar contents of black ginseng jelly were significantly increased(+O.05). We noted that the luminance and Hunter`s b values of jelly samples were decreased according to black ginseng extract was increased, but in Hunter`s a values 0.5% black ginseng jelly was the highest of the all. With regard to the mechanical properties of the black ginseng jelly samples, the score of hardness, gumminess and chewiness were significantly increased. In color, taste and overall quality, the score of jelly with 1.0% black ginseng extract was significantly increased than those of the all.

흑삼 및 흑마늘을 이용한 블랙잼 유동특성 모니터링

이기동(Gee-Dong Lee) 한국응용과학기술학회 (구.한국유화학회) 2020 한국응용과학기술학회지 Vol.37 No.2

본 연구는 흑삼과 흑마늘을 이용하여 블랙잼을 제조하면서 물성의 변화를 모니터링 해 보았다. 블랙잼의 배합 조건은 흑삼(X 1 , 30-54 g), 흑마늘(X 2 , 75-135 g), 펙틴 4.5 g, 사과페이스트 270 g 및 프럭토올리고당 360 g이며, 반응 변수로는 탄력성, 응집성, 씹힘성, 깨짐성 및 부착성으로 반응표면분석을 실시하였다. 탄력성, 응집성, 씹힘성, 깨짐성 및 부착성에 대한 회귀식의 R 2 는 각각 0.8948, 0.9103, 0.9032, 0.9097, 0.8561로 5∼10%의 유의수준에서 유의성이 인정되었다. 블랙잼의 탄력성이 가장 높은 흑삼 및 흑마늘 배합조건과 그 탄력성은 흑삼 함량 54.00 g 및 105.83 g에서 194.39%로 나타났으며, 탄력성이 가장 낮은 흑삼 및 흑마늘 배합조건과 그 탄력성은 흑삼 함량 31.48 g 및 119.43 g에서 164.11% 로 나타났다. 블랙잼의 응집성이 가장 높은 흑삼 및 흑마늘 배합조건과 그 응집성은 흑삼 함량 48.85 g 및 129.62 g에서 40.96%로 나타났다, 응집성이 가장 낮은 흑삼 및 흑마늘 배합조건과 그 응집성은 흑삼 함량 50.06 g 및 82.77 g에서 32.96%로 나타났다. 블랙잼의 씹힘성이 가장 높은 흑삼 및 흑마늘 배합조건과 그씹힘성은 흑삼 함량 42.95 g 및 106.83 g에서 43.19 g로 나타났다. 블랙잼의 깨짐성이 가장 높은 흑삼 및흑마늘 배합조건과 그 깨짐성은 흑삼 함량 32.10 g 및 88.04 g에서 16,874 g로 나타났으며, 깨짐성이 가장 낮은 흑삼 및 흑마늘 배합조건과 그 깨짐성은 흑삼 함량 50.53 g 및 83.91 g에서 678 g로 나타났다. 블랙잼의 부착성이 가장 높은 흑삼 및 흑마늘 배합조건과 그 부착성은 흑삼 함량 32.91 g 및 124.60 g에서 14.06 g로 나타났다. 이상의 결과로부터 흑삼과 흑마늘의 배합비와 블랙잼의 물성의 관계를 조사함으 로서 건강기능성 소재를 이용하여 누구나 즐겨 먹을 수 있는 블랙잼을 제조할 수 있을 것으로 여겨진다. This study monitored the rheological properties of jams manufactured using the black ginseng and black garlic. The conditions for mixing black jam were black ginseng (X 1 , 30-54 g), black garlic (X 2 , 75-135 g), pectin 4.5 g, apple paste 270 g, and fructo-oligosaccharide 360 g. The response surface analysis was performed with springiness, cohesiveness, chewiness, brittleness and gumminess. The R 2 s of the regression equation for springiness, cohesiveness, chewiness, brittleness and gumminess were recognized at a significant level of 5 to 10 %, with 0.8948, 0.9103, 0.9032, 0.9097, and 0.8561, respectively. The combination conditions of black ginseng and black garlic with the highest springiness of black jam were found to be 194.39% (springiness) with black ginseng 54.00g and black garlic 105.83g, while the conditions of black ginseng and black garlic mixing with the lowest springiness were found to be 164.11% with black ginseng 31.48g and black garlic 119.43 g. The mixing conditions of black ginseng and black garlic with the highest cohesiveness of black jam and its consistency were 40.96% (cohesiveness) with black ginseng 48.85g and black garlic 129.62g, while black ginseng and black garlic combination conditions with the lowest cohesiveness were found to be 32.96% with black ginseng 50.06g and black garlic 82.77g. Black ginseng and black garlic mixing conditions, which have the highest chewiness of black jam, was 43.19 g (chewiness) from black ginseng 42.95 g and black garlic 106.83 g. Black ginseng and black garlic mixing conditions and their brittleness were found to be the highest in black ginseng 32.10 g and black garlic 88.04 g to 16,874 g. Black ginseng and black garlic mixing conditions and their brittleness were found to be 678 g from black ginseng 50.53 g and black garlic 83.91 g. Black ginseng and black garlic mixing conditions and their gumminess were 14.06 g with black ginseng content of 32.91 g and black garlic content of 124.60 g. By examining the relationship between black ginseng/black garlic ratio and the rheological property of black jam from above results, it is believed that black jam can be produced for anyone to enjoy using health function material.

포도주스 침지 제조 흑삼의 Ginsenoside Rg₃ 함량 변화와 Acetylcholinesterase 억제효과

이미라(Mi-Ra Lee),윤범식(Beom-Sik Yun),손백신(Bai-Shen Sun),류뢰(Lei Liu),장동량(Dong-Liang Zhang),왕춘년(Chun-Yan Wang),왕젠(Zhen Wang),이선영(Sun-young Ly),모은경(Eun-Kyung Mo),성창근(Chang-keun Sung) 고려인삼학회 2009 Journal of Ginseng Research Vol.33 No.4

흑삼의 속성제조와 ginsenoside Rg₃ 함량을 극대화하고자 흑삼 제조시 포도주스에 24시간 침지한 후 120℃에서 30분간 3회 반복 증숙하여 흑삼을 제조한 후 HPLC 방법을 이용하여 ginsenosides를 분석하였다. 포도주스에 침지하여 제조한 흑삼의 ginsenoside Rg3 함량은 10.91 ㎎/g으로 구증구포 방법으로 제조한 흑삼보다 약 2배 가량 함량이 증가되었다. 총 사포닌 함량은 14.97 ㎎/g으로 전통적인 구증구포 방식으로 제조한 흑삼 (12.79 ㎎)보다 그 함량이 높았다. 흑삼의 단회투여 (200 ㎎/㎏, p.o.)에 의한 뇌조직 AChE 활성은 투여 24시간 후에 유의적으로 억제되는 효과를 보여주었다. 따라서 본 연구에 적용한 새로운 제조방법은 ginsenoside Rg₃를 강화하는 흑삼의 속성제조에 효과적인 방법으로 판단된다. 또한, AChE 활성억제를 통해 흑삼이 뇌기능 개선에 대한 잠재적인 효능을 가지고 있는 것으로 사료된다. This study was conducted to develop a new method for enhancing ginsenoside Rg₃, which is abundant in black ginseng. The cognition-enhancing effect of black ginseng extract was investigated via the assay of acetylcholinesterase (AChE) activity. Black ginseng I was prepared through the traditional method (by steaming and drying nine times repetitions). Black ginseng Ⅱ, on the other hand, was prepared by steaming the ginseng three times at 120℃ for 30 min after soaking it in grape juice for 24 h. The ginsenosides of white, red, and black ginseng Ⅰ, and Ⅱ were investigated using the HPLC method, respectively. In black ginseng Ⅱ, the ginsenoside Rg₃ contents, which cannot be found in white ginseng, amounted to 10.91 ㎎/g, approximately 18 times more than that in red ginseng. In the in-vivo study, black ginseng extract (200 ㎎/㎏, p.o.) inhibited the AChE activity after 24 h by a single administration in the brain. Thus, the new manufacturing method for black ginseng was found to more effective in the conversion of ginsenoside Rg₃ compared to the traditional method. Black ginseng may also have the effect of preventing the cognitive impairment induced by cholinergic dysfunction.

Characterization of Black Ginseng Extract with Acetyl- and Butyrylcholin-esterase Inhibitory and Antioxidant Activities

Beom Sik Yun,Mi Ra Lee,Chang Jin Oh,Jeong Hee Cho,Chun Yan Wang,Li Juan Gu,Eun Kyung Mo,Chang Keun Sung 고려인삼학회 2010 Journal of Ginseng Research Vol.34 No.4

Black ginseng and white ginseng were extracted with 80% ethanol and evaluated for relative ginsenoside composition, acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory activities, and antioxidant properties. As analyzed by HPLC, black ginseng contained a greater proportion of ginsenoside Rg₃ compared to white ginseng. White ginseng was characterized by undetectable ginsenoside Rg₃ but it contained more total ginsenosides than black ginseng. Black ginseng extract exhibited higher (p<0.05) free radical-scavenging activity, as well as higher antioxidant activities than white ginseng against 2,2-diphenyl-1-picryl-hydrazyl, superoxide dismutase, and xanthine oxidase, despite the fact that the total saponin content was higher in white ginseng than black ginseng. In addition, the black ginseng extract displayed greater AChE and BChE inhibitory activities. These results suggest that black ginseng has stronger effects on anti-oxidation and AChE and BChE inhibition than white ginseng.

Ginsenosides Rg5 and Rk1, the skin-whitening agents in black ginseng

Jin, Yan,Kim, Ji Hye,Hong, Hee-Do,Kwon, Jeonghun,Lee, Eun Jung,Jang, Mi,Lee, Sung-Young,Han, Ah-Ram,Nam, Tae Gyu,Hong, Seok Kyu,Huh, Tae-Lin,Kang, Nam Joo,Lim, Tae-Gyu Elsevier 2018 Journal of Functional Foods Vol.45 No.-

<P><B>Abstract</B></P> <P>Ginseng (<I>Panax ginseng</I> Meyer) is widely used to treat various chronic disorders. Black ginseng is obtained by repeated steaming and drying of raw ginseng, which turn it black and convert the ginsenoside compounds present into more potent bioactive ingredients. The effects of a black ginseng extract on melanin production and skin whitening were evaluated in vitro as well as <I>in vivo</I> using human skin and zebrafish embryos. Tyrosinase activity decreased when steamed and dried ginseng was used. A pronounced skin-whitening effect was observed in our clinical study, and inhibition of melanin activity and tyrosinase levels was confirmed in zebrafish embryos. Two ginsenosides specific to black ginseng activate the MEK-ERK signaling pathway and are the main factors responsible for skin whitening. The skin-whitening effects of black ginseng are associated with the formation of two ginsenosides, indicating that black ginseng can be used as a novel skin-whitening agent in cosmetic products.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Inhibitory effect of black ginseng extract on melanin production on B16F10 and zebrafish model. </LI> <LI> Skin whitening activity of black ginseng extract on human skin. </LI> <LI> Ginsenoside Rg5 and Rk1, bioactive compounds in black ginseng extract for skin whitening activity. </LI> <LI> Activation MEK-ERK signaling pathway, a underlying mechanism for skin whitening activity of black ginseng extract. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Characterization of Black Ginseng Extract with Acetyl- and Butyrylcholinesterase Inhibitory and Antioxidant Activities

Yun, Beom-Sik,Lee, Mi-Ra,Oh, Chang-Jin,Cho, Jeong-Hee,Wang, Chun-Yan,Gu, Li Juan,Mo, Eun-Kyung,Sung, Chang-Keun The Korean Society of Ginseng 2010 Journal of Ginseng Research Vol.34 No.4

Black ginseng and white ginseng were extracted with 80% ethanol and evaluated for relative ginsenoside composition, acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory activities, and antioxidant properties. As analyzed by HPLC, black ginseng contained a greater proportion of ginsenoside $Rg_3$ compared to white ginseng. White ginseng was characterized by undetectable ginsenoside $Rg_3$ but it contained more total ginsenosides than black ginseng. Black ginseng extract exhibited higher (p<0.05) free radical-scavenging activity, as well as higher antioxidant activities than white ginseng against 2,2-diphenyl-1-picryl-hydrazyl, superoxide dismutase, and xanthine oxidase, despite the fact that the total saponin content was higher in white ginseng than black ginseng. In addition, the black ginseng extract displayed greater AChE and BChE inhibitory activities. These results suggest that black ginseng has stronger effects on anti-oxidation and AChE and BChE inhibition than white ginseng.

포도주스 침지 제조 흑삼의 Ginsenoside Rg₃ 함량 변화와 Acetylcholinesterase 억제효과

이미라,윤범식,손백신,류뢰,장동량,왕춘년,왕젠,이선영,모은경,성창근,Lee, Mi-Ra,Yun, Beom-Sik,Sun, Bai-Shen,Liu, Lei,Zhang, Dong-Liang,Wang, Chun-Yan,Wang, Zhen,Ly, Sun-Young,Mo, Eun-Kyung,Sung, Chang-Keun 고려인삼학회 2009 Journal of Ginseng Research Vol.33 No.4

흑삼의 속성제조와 ginsenoside $Rg_3$ 함량을 극대화하고자 흑삼 제조시 포도주스에 24시간 침지한 후 $120^{\circ}C$에서 30분간 3회 반복 증숙하여 흑삼을 제조한 후 HPLC 방법을 이용하여 ginsenosides를 분석하였다. 포도주스에 침지하여 제조한 흑삼의 ginsenoside $Rg_3$ 함량은 10.91 mg/g으로 구증구포 방법으로 제조한 흑삼보다 약 2배 가량 함량이 증가되었다. 총 사포닌 함량은 14.97 mg/g으로 전통적인 구증구포 방식으로 제조한 흑삼 (12.79 mg)보다 그 함량이 높았다. 흑삼의 단회투여 (200 mg/kg, p.o.)에 의한 뇌조직 AChE 활성은 투여 24시간 후에 유의적으로 억제되는 효과를 보여주었다. 따라서 본 연구에 적용한 새로운 제조방법은 ginsenoside $Rg_3$를 강화하는 흑삼의 속성제조에 효과적인 방법으로 판단된다. 또한, AChE 활성억제를 통해 흑삼이 뇌기능 개선에 대한 잠재적인 효능을 가지고 있는 것으로 사료된다. This study was conducted to develop a new method for enhancing ginsenoside $Rg_3$, which is abundant in black ginseng. The cognition-enhancing effect of black ginseng extract was investigated via the assay of acetylcholinesterase (AChE) activity. Black ginseng I was prepared through the traditional method (by steaming and drying nine times repetitions). Black ginseng II, on the other hand, was prepared by steaming the ginseng three times at $120^{\circ}C$ for 30 min after soaking it in grape juice for 24 h. The ginsenosides of white, red, and black ginseng I, and II were investigated using the HPLC method, respectively. In black ginseng II, the ginsenoside $Rg_3$ contents, which cannot be found in white ginseng, amounted to 10.91 mg/g, approximately 18 times more than that in red ginseng. In the in-vivo study, black ginseng extract (200 mg/kg, p.o.) inhibited the AChE activity after 24 h by a single administration in the brain. Thus, the new manufacturing method for black ginseng was found to more effective in the conversion of ginsenoside $Rg_3$ compared to the traditional method. Black ginseng may also have the effect of preventing the cognitive impairment induced by cholinergic dysfunction.

원료삼 크기와 형태가 흑삼의 품질에 미치는 영향

김염,김경탁,임태규,장미,조장원,이영경,홍희도,Jin, Yan,Kim, Kyung-Tack,Lim, Tae-Gyu,Jang, Mi,Cho, Chang-Won,Rhee, Young Kyoung,Hong, Hee-Do 한국식품영양학회 2016 韓國食品營養學會誌 Vol.29 No.5

흑삼은 고기능성 신규 인삼제품으로 높은 관심의 대상이 되고 있지만, 흑삼제조와 관련된 기초 연구는 아직 미미한 실정이다. 본 연구에서는 원료삼의 외형적 특성이 흑삼제품의 품질에 미치는 영향을 살펴보기 위하여 동일지역, 동일시기에 수확된 5년근 수삼을 시중에서 구입한 후, 크기별(중, 대, 특대) 또는 형태별(직삼, 난발삼)로 분류하고, 흑삼 제조시의 수율과 당류, 폴리페놀 및 진세노사이드 등 주요 이화학적 성분 및 항산화 활성을 비교하였다. 원료삼의 크기별 이화학적 특성을 살펴본 결과, 흑삼의 산성다당체, 흑삼특이 미량 진세노사이드인 Rk3, Rh4, Rg5, Rk1의 함량은 크기등급 L에서 다소 높았으나, 전체적으로 원료삼의 크기에 따른 흑삼의 이화학적 특성은 유의적인 차이는 없었다. 원료삼의 형태에 따른 흑삼의 이화학적 특성 분석결과에서는 난발삼의 산성다당체, 흑삼 특이 미량 진세노사이드인 Rk3, Rh4, Rg5, Rk1의 함량이 직삼보다 다소 높은 경향을 나타내었으나, 폴리페놀 함량과 항산화 활성은 형태에 따른 유의적인 차이가 없었다. 전반적으로 흑삼 제조 시 원료삼의 크기는 흑삼품질에 큰 영향을 미치지 않는 것으로 판단되었으나, 원료삼의 형태는 주요 활성성분인 산성다당체, 진세노사이드 함량에 다소 영향을 미치는 것으로 판단되었다. 본 연구결과는 고품질 흑삼제조를 위한 기초자료로 활용 가능할 것이라 기대된다. Current study was performed to investigate the effect of morphological properties of black ginseng such as size and shape on the quality of black ginseng. The raw ginsengs were separated based on size (medium, large, and extra-large) and shape (straight ginseng, fibrous root ginseng). Subsequently, the raw ginsengs were steamed at $95^{\circ}C$ for 3 h and dried in the presence of heated air at $50^{\circ}C$ for 30 h. This process was repeated nine times for black ginseng production. The physiochemical properties such as the content of acidic polysaccharides, ginsenosides, and antioxidative activity were evaluated. Although minor difference in physiochemical properties such as acidic polysaccharide content in raw ginseng was observed, no statistical difference in the content of acidic polysaccharides, total phenols, and ginsenosides was observed during final black ginseng production based on size classification. The minor ginsenosides in fibrous root black ginseng, such as Rk3, Rh4, Rg3, Rk1, and Rg5 were higher in content than straight black ginseng. However, no correlation between the shape of ginseng and total phenol content and antioxidative activity was observed. Therefore, present results demonstrate that the difference in ginseng size in same-age and -production area does not affect the quality of black ginseng. Furthermore, difference in ginseng shape does not influence the overall quality of black ginseng. It is hypothesized that this study would be considered as supportive data for the production of high-quality black ginseng.

Current Status of Korean Ginseng Products and Trends in Enhanced Functional Ginseng Products

Byungdae Lee,Tae-Eun Kwon,Hoon-Il Oh,Ho-jung Yoon 고려인삼학회 2024 인삼문화 Vol.6 No.1

The abolishment of the red ginseng monopoly act by the Korean government in 1996 resulted in a drastic change in the Korean ginseng industry, leading to a significant increase in the market size and consumption of ginseng products. Red ginseng is most popular type, with approximately 74% of harvested fresh ginseng being processed into various red ginseng products. Since 1997, there has been a substantial increase in the cultivation of ginseng for production of red ginseng, which, in turn, has contributed to the proliferation of ginseng processing companies. To investigate the products of ginseng manufacturing businesses, we select 200 companies primarily engaged in ginseng processing or specializing solely in ginseng. Our survey on the status of ginseng industry covered 8 different categories. 1) Root ginseng: There were 66 companies involved in manufacturing red ginseng root, accounting for 33.0% of all surveyed companies. This was followed by black ginseng root with 36 companies (18.0%) and red ginseng fine roots with 22 companies (11%). 2) Red ginseng products: A total of 144 companies were involved in manufacturing red ginseng pouches, making it the most common product category. This was closely followed by 142 companies producing pure(100%) red ginseng extract concentrate. 3) Fermented red ginseng products: Companies producing fermented red ginseng extract concentrate products were the most numerous, totaling 26. Following this, companies producing fermented red ginseng stick and pouch products were next in line. 4) Ginseng products: There were 15 companies involved in the production of ginseng products, with the majority focusing on ginseng tea. 5) Black ginseng products: Companies producing black ginseng extract concentrate were the most numerous, with 31 companies, followed by 26 companies producing black ginseng extract pouches. 6) Taegeuk ginseng products: Only 5 companies were involved in the production of taegeuk ginseng products. 7) Fermented black ginseng, and 8) Ginseng berry products: These categories are manufactured by less than 5 companies each. However, the variety in ginseng berry products suggests the potential for future growth. In the 2000s, a trend emerged with the development of new processed products aimed at enhancing the functional components of red ginseng, and these products have captured the attention of consumers. However, this study primarily focuses on black ginseng, fermented red ginseng/fermented black ginseng, and ginseng berry products as they have exerted a significant influence on the overall ginseng industry.

정상 식이 수컷 흰쥐에서 흑삼이 체중 및 지질에 미치는 영향

송규용(Gyu Yong Song),오한진(HAn Jin Oh),노성수(Seong Soo Roh),서영배(Young Bae Seo),박용진(Yong Jin Park),명창선(Chang-Seon Myung) 대한약학회 2006 약학회지 Vol.50 No.6

Previously, we have reported that black ginseng was produced by nine-time repeated steaming 5-year fresh ginsengs at 95℃ for 3 hr in pottery apparatus and drying at 60℃ for 12~36 hr and had an outstanding anticancer activity. This study was designed to examine the ability of black ginseng to suppress body weight gain and appetite, and to investigate the effect of black ginseng on lipid profile in male rats fed normal diets. The extract of black ginseng was administered daily for 3 weeks, and the changes of body weight and food intake were measured. After administering the extracts for 3 weeks, the fat weight and serum lipid levels were also measured. Black ginseng persistently suppressed weigh gain and food intake was also reduced, but not significantly. The weight of both epididymal and abdominal fats was decreased in black ginseng-treated rats. Serum triglyceride level in rats administered with black ginseng was also significantly lowered. Therefore, these results reveal that the repeated administration of black ginseng causes a suppression body weigh gain by decreasing triglyceride level in blood and body fat deposit.