A MULTI-STORY FIRE IN HIGH-RISE APARTMENT BUILDING DEVELOPED THROUGH BALCONIES - INVESTIGATION AND EXPERIMENTS -

Hasemi,Yuji,Hayashi,Yoshihiko,Hokugo,Akihiko,Yoshida,Masashi 한국화재소방학회 1997 한국화재소방학회 학술대회 논문집 Vol.1997 No.-

Summary of experiments for the investigation of a fire which caused an upward fire spread for over 12 floors through balconies in a high-rise apartment complex is reported. The experiments include indoor tests to obtain fire properties of vertical PMMA fences and outdoor ones with a full scale model of the balcony. The test results suggest significance of the increase of total flame height by the merging of flames and a cooperative effect of the burning of the PMMA fence and combustibles on the balconies for the generation of a tall flame enough to cause ignition on the upper floors.

Load Management using Heat-Pump Water Heater and Electric Vehicle Battery Charger in Distribution System with PV

Hanai, Yuji,Yoshimura, Kazuaki,Matsuki, Junya,Hayashi, Yasuhiro The Korean Institute of Electrical Engineers 2011 The Journal of International Council on Electrical Vol.1 No.2

This paper proposes a new load management method using Heat Pump Water Heater (HPWH) and Electric Vehicle Battery Charger (EV-BC) in a distribution system with a high penetration of Photovoltaic generation system (PV). The proposed method implements a classification of the controllable loads (HP-WH, EV-BC) by distribution section and optimizes operation modes of the classified controllable loads. A distribution section enclosed with two or three IT switches, which are sectionalizing switches with sensors, is defined as a load management cluster, and a large number of controllable loads in a distribution feeder are classified into three clusters. All the customers with controllable loads determine beforehand two operation modes which achieve the satisfactory convenience and energy conservation; one is a nighttime consumption pattern and another is a daytime consumption pattern. Distribution System Operator (DSO) can collect the customers' information such as address codes and operation plans, and select either of the operation modes in each distribution section. The optimal combination of these operation modes is determined by Optimal Power Flow calculation (OPF). The objective is to improve load factor while keeping a power quality of a distribution system. The validity of the proposed method is verified through computer simulations using distribution network model.

Clinical Significance of Proliferative Activity in the Residual Cancerous Foci in Conversion Therapy for Colorectal Liver-confined Metastasis

Hiromitsu Hayashi,Yasuo Sakamoto,Yuji Miyamoto,Toru Beppu,Hidetoshi Nitta,Kuroki Hideyuki,Shigeki Nakagawa,Takaaki Higashi,Akira Chikamoto,Hideo Baba 대한종양외과학회 2014 대한임상종양학회 학술대회지 Vol.2014 No.3

A Basic Study of a Coordinated Control Method for Heat Pump Water Heaters and Electric Vehicle Battery Chargers in Residence with PV Systems

Hanai, Yuji,Yoshimura, Kazuaki,Matsuki, Junya,Hayashi, Yasuhiro The Korean Institute of Electrical Engineers 2011 The Journal of International Council on Electrical Vol.1 No.1

In recent years, Photovoltaic generation (PV) has gained a lot of attention due to its potential for contributing to part of the solution to global warming. In addition, the introduction of PV increased rapidly in many houses. If PV is introduced on a large scale, this may cause the allowable voltage produced by each customer to increase thereby deviate the allowable voltage. The installation of a storage battery can decrease the PV's output and control power surges. However, there are serious problems in respect to the installation cost and storage space of batteries. Therefore in this research, the authors propose and verify a new coordinated control method of heat-pump water heaters and electric vehicle battery chargers in a residence with a PV system. The proposed method compensates for the voltage rises and drops deviating from the allowable range by coordinated control of heat-pump water heaters and electric vehicle battery chargers. In order to verify the proposed method, an experimental simulation using an analog type distribution system simulator is carried out.

Loss Analysis of Hybrid Electric Vehicle Inverters

Fumio Asakura,Norihito Kimura,Yuji Hayashi 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5

The analysis of electrical losses of hybrid electric vehicle (HEV) inverters is significant for improved fuel efficiency for HEVs. To analyze the breakdown of electrical losses in LA-4 Cycle or under other similar driving conditions, measurement and calculation methods have been developed to ascertain losses attributable to different parts of HEV inverters. To determine electrical losses, the methods use voltages and currents in HEV inverters measured with a vehicle-mounted virtual and real simulator (VRS)

Biochemical Properties of Two Lipoxygenases from Pleurotus ostreatus

Mio Kobayashi,Shunya Hayashi,Yuji Tasaki 한국버섯학회 2017 버섯 Vol.21 No.2

The characteristic aroma of mushrooms is one of their attractive elements as food materials. The major aroma compound in most mushrooms is 1-octen-3-ol. The biosynthesis of 1-octen-3-ol starts with the oxidation of linoleic acid by lipoxygenase (LOX). The resultant intermediate hydroperoxide is then cleaved by hydroperoxide lyase. LOX is a non-heme iron-containing dioxygenase widely found in plants, animals, fungi, and bacteria. It catalyzes the insertion of molecular oxygen into polyunsaturated fatty acids containing Z,Z-1,4-pentadiene moieties, such as linoleic acid, linolenic acid, and arachidonic acid, yielding the corresponding hydroperoxides. Two LOX genes, Polox1 and Polox2, have been isolated from P. ostreatus, which has higher LOX activity than other edible mushrooms. Polox1 and Polox2 were found to show different expression patterns during the development of the fruiting body. However, the biochemical properties of PoLOX1 and PoLOX2, encoded by Polox1 and Polox2, respectively, have been not fully elucidated. In this study, we engineered these two LOX genes of P. ostreatus into a heterologous host, Escherichia coli, and characterized the recombinant proteins. The coding regions of Polox1 and Polox2 were amplified by RT-PCR from the total RNA of P. ostreatus PC15 mycelia. The RT-PCR products were digested with appropriate restriction enzymes and ligated into an expression vector (pET-16b). The resultant plasmids were introduced into E. coli BL21 (DE3) via transformation. Polox1 and Polox2 were then expressed by induction at 15°C with 0.4 mM IPTG for 18 h. The cells were harvested by centrifugation and resuspended in 10 mM potassium phosphate buffer (pH 7.0). The cell suspension was sonicated and again centrifuged at 15,000 ×g for 20 min at 4 °C. The resultant cell-free extract was used for subsequent experiments. Recombinant PoLOX1 and PoLOX2 were confirmed by SDS-PAGE analysis of the cell-free extract. PoLOX1 and PoLOX2 were estimated to have molecular weights of approximately 76,000 Da and 78,000 Da, respectively. The LOX activity was determined with linoleic acid as a substrate by a spectrophotometric procedure based on the formation of conjugated dienes. To characterize the biochemical properties of PoLOX1 and PoLOX2, in vitro enzymatic assays were performed using the total cell protein from E. coli expressing the two Polox genes, with linoleic acid as a substrate. The optimum pH of recombinant PoLOX1 and PoLOX2 was 7.5 and 5.5, respectively; the optimum temperatures of recombinant PoLOX1 and PoLOX2 were 55 °C and 30 °C, respectively. Recombinant PoLOX1 and PoLOX2 were stable at pH 5.0-9.0 and 6.0-8.0, respectively; recombinant PoLOX1 and PoLOX2 were relatively stable below 50 °C and 40 °C, respectively. Thus, PoLOX1 had higher thermal and pH stability than PoLOX2. The calculated Km values of PoLOX1 and PoLOX2 were 121 μM and 249 μM, respectively. The calculated Vmax values of PoLOX1 and PoLOX2 were 17.2 μmol/mg・min and 17.5 μmol/mg・min, respectively. These results indicated that PoLOX1 had a higher affinity for linoleic acid than PoLOX2. Collectively, our findings suggested that there were some differences between the biochemical properties of PoLOX1 and PoLOX2.

Effect of l-phenylalanine Supplementation on Methyl Cinnamate Production and Phenylalanine Ammonia-lyase Expression in the Mycelium of Tricholoma matsutake

Miho Kato,Shunya Hayashi,Yuji Tasaki 한국버섯학회 2017 버섯 Vol.21 No.2

Tricholoma matsutake is a representative mushroom species with a characteristic pleasant aroma. The characteristic aroma component is methyl cinnamate, which is also produced in many plants. In basil, cinnamic acid is produced from l-phenylalanine (l-Phe) by phenylalanine ammonia-lyase (PAL) and converted to methyl cinnamate by a cinnamate/p-coumarate carboxyl methyltransferase. Two PAL genes, Tmpal1 and Tmpal2, have been isolated from T. matsutake. In this study, we aimed to clarify the relationships between l-Phe, methyl cinnamate production, and PAL expression in the mycelium of T. matsutake strain NBRC 30605. For this purpose, methyl cinnamate content, PAL activity, and transcript levels of Tmpal1 and Tmpal2 were examined in the mycelia of T. matsutake supplemented with l-Phe. The mycelia were cultured in 20 mL of a liquid medium (2% glucose, 0.15% yeast extract, and 0.15% Bacto Soytone) at 20 °C for 45 d, supplemented with 0.5-6 mM l-Phe, and then grown for a further 15 d. Mycelia cultured without l-Phe supplementation for 60 d in the medium were used as a control. Crude extracts were prepared from the mycelia harvested for enzymatic, protein, and methyl cinnamate assays. Methyl cinnamate was measured using gas chromatography. PAL activity was assayed by measuring the rate of trans-cinnamic acid formation as the absorbance at 290 nm (ɛ290 = 10,000 M−1 cm−1). The transcript levels of Tmpal1 and Tmpal2 were examined by performing real-time reverse transcriptase-quantitative PCR on the total RNA. Methyl cinnamate was detected in very low levels in cultures without l-Phe supplementation, but its content per mg of protein increased markedly with increasing concentrations of l-Phe, especially at 4-6 mM. When 6 mM l-Phe was added to the culture medium, the methyl cinnamate content was approximately 55-fold higher than that of the control sample. The specific activity of PAL also increased in cultures supplemented with l-Phe, especially at 4-6 mM. When l-Phe was added to the culture medium, the methyl cinnamate content in the mycelia was relatively well correlated with PAL activity. These results indicated that supplementation with l-Phe, a precursor of methyl cinnamate, increases the specific activity of PAL, leading to an increase in methyl cinnamate production in the mycelia of T. matsutake. The transcript level of Tmpal1 did not change markedly with l-Phe supplementation. In contrast, the transcript level of Tmpal2 increased greatly in cultures supplemented with 4-6 mM l-Phe. These results suggested that the expression of Tmpal1 and Tmpal2 was controlled by different regulatory mechanisms and that they may have different biological functions in T. matsutake. In addition, the pattern of PAL activity in the presence of l-Phe was similar to that of the transcript level of Tmpal2, but not Tmpal1, suggesting that the increase in PAL activity was dependent on the increased transcription of Tmpal2.

Heterogeneous interaction network of yeast prions and remodeling factors detected in live cells

( Chan-gi Pack ),( Yuji Inoue ),( Takashi Higurashi ),( Shigeko Kawai-noma ),( Daigo Hayashi ),( Elizabeth Craig ),( Hideki Taguchi ) 생화학분자생물학회(구 한국생화학분자생물학회) 2017 BMB Reports Vol.50 No.9

Budding yeast has dozens of prions, which are mutually dependent on each other for the de novo prion formation. In addition to the interactions among prions, transmissions of prions are strictly dependent on two chaperone systems: the Hsp104 and the Hsp70/Hsp40 (J-protein) systems, both of which cooperatively remodel the prion aggregates to ensure the multiplication of prion entities. Since it has been postulated that prions and the remodeling factors constitute complex networks in cells, a quantitative approach to describe the interactions in live cells would be required. Here, the researchers applied dual-color fluorescence cross-correlation spectroscopy to investigate the molecular network of interaction in single live cells. The findings demonstrate that yeast prions and remodeling factors constitute a network through heterogeneous protein-protein interactions. [BMB Reports 2017; 50(9): 478-483]

흉부(胸部) 경선질촬영(硬線質撮影)에 있어서 피폭선양(被曝線量)의 저감(低減)에 관(關)한 연구(硏究)

임태랑,석전유치,전전미향,앵정달야,김정웅,황종선,Hayashi, Taro,Ishida, Yuji,Maeda, Mika,Sakurai, Tatsuya,Kim, Chung-Woon,Hwang, Jong-Sun 대한방사선과학회 1991 방사선기술과학 Vol.14 No.2

Experiment was conducted on the image quality and exposure dose following replacement of $CaWO_4$ system screen BH-III and BX-III which have so far been used for high-voltage hard-radiation quality radiography, with rare earth system screen KO750, combined with high contrast film SRH, while additional filter was altered, Cu 0.8 mm+Al 1.4 mm(HVL : Al 8.8 mm), Cu 1.3 mm+Al 1.0 mm(HVL : Al 10.6 mm) and Cu 1.8 mm+Al 1.5 mm(HVL : Al 11.4 mm). AS a result, visual evaluation did not detect extreme changes in image quality under the respective condition(HVL : $Al\;8.8\;mm{\sim}Al\;11.4\;mm$). It was noted, however, that surface exposure dose declined with an increase in the thickness of the additional filter, as it was $18.9\;{\mu}Gy$ at HVL Al 8.8 mm, $17.5\;{\mu}Gy$ at Al 10.6 mm and $15.7\;{\mu}Gy$ at Al 11.4 mm. Considering the limited rating of X-ray equipment and wear of machinery, however, the range of $Cu\;1.3\;mm{\sim}1.8\;mm+Al\;1.0\;mm{\sim}1.5\;mm(1/16\;VL{\sim}1/32\;VL)$ seemed to be a limit.

Unilateral rostral mandibulectomy for gingival vascular hamartoma in two calves

Takeshi Tsuka,Yoshiharu Okamoto,Naoki Yamamoto,Keiji Hayashi,Takehito Morita,Yuji Sunden,Yusuke Murahata,Kazuo Azuma,Tomohiro Osaki,Norihiko Ito,Tomohiro Imagawa 대한수의학회 2018 Journal of Veterinary Science Vol.19 No.4

A 2-month-old female Holstein calf and a 5-month-old female Japanese black calf presented with gingival vascular hamartoma located in the interdental space between the second and third mandibular incisors in the right and left mandibles, respectively. On radiographic or computed tomographic images, osteolytic changes appeared within the mandibular bones adjacent to the masses. The masses were removed along with affected mandibular bone by using unilateral rostral mandibulectomy. After surgery, both cases exhibited a normal appetite and grew normally, with no cosmetic changes or recurrences. Unilateral rostral mandibulectomy can be applied for invasive gingival vascular hamartomas associated with osteolytic changes.