Comparison of trophic factors changes in the hippocampal CA1 region between the young and adult gerbil induced by transient cerebral ischemia.

Yan, Bing Chun,Park, Joon Ha,Kim, Sung Koo,Choi, Jung Hoon,Lee, Choong Hyun,Yoo, Ki-Yeon,Kwon, Young-Geun,Kim, Young-Myeong,Kim, Jong-Dai,Won, Moo-Ho Kluwer Academic/Plenum Publishers 2012 Cellular and molecular neurobiology Vol.32 No.8

<P>In the present study, we investigated neuronal death/damage in the gerbil hippocampal CA1 region (CA1) and compared changes in some trophic factors, such as brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF) and vascular endothelial growth factor (VEGF), in the CA1 between the adult and young gerbils after 5 min of transient cerebral ischemia. Most of pyramidal neurons (89%) were damaged 4 days after ischemia-reperfusion (I-R) in the adult; however, in the young, about 59% of pyramidal neurons were damaged 7 days after I-R. The immunoreactivity and levels of BDNF and VEGF, not GDNF, in the CA1 of the normal young were lower than those in the normal adult. Four days after I-R in the adult group, the immunoreactivity and levels of BDNF and VEGF were distinctively decreased, and the immunoreactivity and level of GDNF were increased. However, in the young group, all of their immunoreactivities and levels were much higher than those in the normal young group. From 7 days after I-R, all the immunoreactivities and levels were apparently decreased compared to those of the normal adult and young. In brief, we confirmed our recent finding: more delayed and less neuronal death occurred in the young following I-R, and we newly found that the immunoreactivities of trophic factors, such as BDNF, GDNF, and VEGF, in the stratum pyramidale of the CA1 in the young gerbil were much higher than those in the adult gerbil 4 days after transient cerebral ischemia.</P>

유통업의 지식경영 도입 방안에 관한 연구

Yoo,Sang-jin,Cha,Young-han 한국산업경영학회 2002 한국산업경영학회 발표논문집 Vol.2002 No.2

In this study, an effective methodology to introduce knowledge management system into the retail business is suggested by overall analysis of examples those were carried out in business area. Different from existing studies, which focus on theoretical aspect only, this study focuses its attention on theoretical & practical aspect simultaneously and tries to find a long-term & integrated knowledge management strategy. With examining the examples of knowledge management, this study deals with the fundamental conception, the procedure and the organization of knowledge management. Moreover, this study practically analyzes the knowledge management system of the company named "H", one of the retail business companies. By clarifying the problems, which is involved in the knowledge management process of the above company, this study suggests an improvement methodology to introduce and settle down knowledge management system into retail business company for the future. This study has good results in the viewpoint that it gives a definite direction of knowledge management introduction. However, there is some limitation such as the insufficiency of examples and the omission of documented analysis. Therefore, this study leaves some task to be achieved in the future.

Haemophilus influenzae Type b 에 대한 급성 후두염의 치료 경험 2 례

이수영(Soo Young Lee),유상호(Sang Ho Yoo),이기선(Ki Sun Lee),홍미애(Mi Ae Hong),김순남(Soon Nam Kim) 대한소아알레르기호흡기학회 1998 소아알레르기 및 호흡기학회지 Vol.8 No.1

Epiglottitis is an uncommon but potentially life threatening infectious disease in young children. And it is rapidly progressing cellulitis of the epiglottis and adjacent structures that has the potential for causing abrupt, complete airway obstruction. The most common cause of acute epiglottitis is Haemophilius influenzae type b (Hib), therefore in USA, the acute epiglottitis is seen less commonly since the wide spread use of immunization against Hib. In Korea, there has been no report of acute epiglottitis caused by known bacterial organism, and a little investigation of the effects of the immunization against Hib, or Hib related respiratory diseases. In this report, we describe two cases of acute epiglottitis caused by Hib, occurred in non-immunized young-male children admitted to Ajou University Hospital. They had visited to the emergency center of Ajou University hospital with the complaints of acute fever, drooling, dysphagia and severe respiratory difficulties. In both cases, we observed the cherry-red colored, severely swollen epiglottis by the direct laryngoscopic examination. Hib was cultured in blood samples from both two cases, and the patients treated successfully by the 3 days of endotracheal intubation and proper antibiotics therapy without any complications such as pneumonia, meningitis, osteomyelitis, or pericarditis.

약년자 폐암의 임상적 관찰

유빈(Bin Yoo),정희순(Hee Soon Chung),한성구(Sung Koo Han),심영수(Young Soo Shim),김건열(Keun Youl Kim),한용철(Yong Chol Han) 대한내과학회 1987 대한내과학회지 Vol.32 No.5

N/A Carcinoma of the lung is generally considered as a disease of middle age and elderly. But lung cancer is now so prevalent that patients below the age of 40 years must be increaseing. We reviewed the records of 483 lung cancer patients who had been diagnosed in the department of internal medicine of Seoul National University Hospital from March 1984 to February 1986, and the clinical features of the young patients under 40 years were compared with those of the control group patients over 40 years. The results are summarized as follows; 1) Seven percent of 34 cases of 483 patients were belong to the young age group patients (less than 40 years). The youngest patient was 22 years old. 2) Male to female ratios of young group and the control group were 3.25:1, 5.9:1 respectively (p>0.05). 3) The mean duration of symptoms from the onset to the definite diagnosis was 2.7 months in young group and 2.3 months in the control group (p>0.3). 4) The most common initial symptoms in the young group were dyspnea (26.5%), hemoptysis (20.5%), cough or sputum (20.5/), chest pain (8.8%) which were also the most common initial symptoms in the control group. (p>0.25) The incidence of superior vena cava syndrome was significantly higher in the young age group (23.5%) than in the control group (7.1%) (p<0.05). 5) In the young group, the proportion of adenocarcinoma (35%) was significantly higher than that of the control group (18%) (p<0.05). 6) The proportion of smoker was significantly lower in the young age group (13 cases, 38.2%) than in the control group (77.7%). The histological type of 13 smokers of the young group was squamous cell carcinoma in 12 cases and adenocarcinoma in 1 case. 7) All non-small cell lung cancer patients in young group were belong to stage III. 8) Distant metastasis rate of the young group (74%) was significantly higher than that of the control group (54.8%) (p<0.05).

Specific proteolysis of the A-kinase-anchoring protein 149 at the Asp582 residue by caspases during apoptosis.

Yoo, Hoesook,Cha, Hwa Jun,Lee, Joonyoung,Yu, Eun-Ok,Bae, Seunghee,Jung, Jin Hyuk,Sohn, Insook,Lee, Su-Jae,Yang, Kwang-Hee,Woo, Sang-Hyeok,Seo, Sung-Keum,Park, In-Chul,Kim, Chong Soon,Jin, Young-Woo,Ah National Hellenic Research Foundation 2008 Oncology reports Vol.19 No.6

<P>A-kinase-anchoring protein 149 (AKAP149) is a member of a structurally diverse, though functionally similar anchoring protein family and is localized to the outer membrane of mitochondria and in the endoplasmic reticulum-nuclear envelope network. AKAP149 plays an important role in controlling the subcellular localization and temporal specificity of protein phosphorylation and mRNA metabolism by tethering kinases and phosphatases, such as protein kinase A and type I protein phosphatase, through its N-terminal protein-binding motifs and mRNAs via its C-terminal RNA-binding motifs. It is well recognized that caspases play a central role in transducing and amplifying the intracellular death signal and that apoptosis is executed as a consequence of caspase-mediated cleavage of multiple cellular substrates. The identification of novel death substrates and elucidation of the consequences of their proteolytic cleavages by caspases are therefore crucial for our understanding of cell death and other biological processes. Herein, we demonstrated that AKAP149 is a direct substrate of active caspase-3, -8 -and -10 in vitro and in vivo. 35S-labeled full-length AKAP149 was completely cleaved in vitro by active caspase-3, -8 and -10 into two fragments of approximately 105 and 45 kDa, while caspase-2 cleaved it partially and caspase-1 did not cleave it at all. AKAP149 was also cleaved by caspases during Fas- and staurosporine-induced apoptosis in Jurkat T and HeLa cells, which were blocked by specific inhibitors of caspase-3 and -8. The specific cleavage site for these caspases was mapped in vitro and in vivo to Asp582 at AKAP149, which is located between the protein kinase A regulatory subunit anchoring and KH RNA-binding domains. In addition, HeLa cells transiently overexpressing AKAP149 D582E mutant were resistant to staurosporine-induced HeLa cell apoptosis. Taken together, these data suggest that AKAP149 activity may be deregulated by caspase-dependent proteolysis during apoptotic cell death and may provide useful information for elucidating the apoptosis signaling pathways in detail.</P>

E-cadherin as a predictive marker of brain metastasis in non-small-cell lung cancer, and its regulation by pioglitazone in a preclinical model.

Yoo, Jin Young,Yang, Seung-Ho,Lee, Jung Eun,Cho, Deog Gon,Kim, Hoon Kyo,Kim, Sung Hwan,Kim, Il Sup,Hong, Jae Taek,Sung, Jae Hoon,Son, Byung Chul,Lee, Sang Won M. Nijhoff ; Kluwer Academic Publishers 2012 Journal of neuro-oncology Vol.109 No.2

<P>It remains unclear whether patients with non-small-cell lung cancer (NSCLC) develop brain metastasis during or after standard therapy. We attempted to identify biological markers that predict brain metastasis, and investigated how to modulate expression of such markers. A case-control study of patients who were newly diagnosed with NSCLC and who had developed brain metastasis during follow-up was conducted between 2004 and 2009. These patients were compared with a control group of patients who had NSCLC but no evidence of brain metastasis. Immunohistochemical analysis of expression of Ki-67, p53, Bcl-2, Bax, vascular endothelial growth factor, epidermal growth factor receptor, caspase-3, and E-cadherin was conducted. The methylation status of the genes for O(6)-methylguanine-DNA-methyltransferase, tissue inhibitor of matrix metalloproteinase (TIMP)-2, TIMP-3, and death-associated protein-kinase was also determined, by use of a methylation-specific polymerase chain reaction. A significantly increased risk of developing brain metastasis was associated with the presence of primary tumors with low E-cadherin expression in patients with NSCLC. We also investigated the effects of pioglitazone, a peroxisome proliferator-activated receptor γ-activating drug, in tumor-bearing mouse models. We found that E-cadherin expression was proportional to pioglitazone exposure time. Interestingly, pioglitazone pretreatment before cancer cell inoculation prevented loss of E-cadherin expression and reduced expression of MMP9 and fibronectin, compared with the control group. E-cadherin expression could be a predictor of brain metastasis in patients with NSCLC. Preventive treatment with pioglitazone may be useful for modulating E-cadherin expression.</P>

A sampling and estimation method for monitoring poultry red mite (Dermanyssus gallinae) infestation on caged-layer poultry farms

Sang-Ik Oh,Ki-Tae Park,Young-Hun Jung,Yoon Jung Do,Chang Yong Choe,Ara Cho,Suhee Kim,Jae-Gyu Yoo 대한수의학회 2020 Journal of Veterinary Science Vol.21 No.3

Background: The poultry red mite, Dermanyssus gallinae, is a serious problem in the laying hen industry worldwide. Currently, the foremost control method for D. gallinae is the implementation of integrated pest management, the effective application of which necessitates a precise monitoring method. Objectives: The aim of the study was to propose an accurate monitoring method with a reliable protocol for caged-layer poultry farms, and to suggest an objective classification for assessing D. gallinae infestation on caged-layer poultry farms according to the number of mites collected using the developed monitoring method. Methods: We compared the numbers of mites collected from corrugated cardboard traps, regarding with length of sampling periods, sampling sites on cage, and sampling positions in farm buildings. The study also compared the mean numbers of mites collected by the developed method with the infestation levels using by the conventional monitoring methods in 37 caged-layer farm buildings. Results: The statistical validation provided the suitable monitoring method that the traps were installed for 2 days on feed boxes at 27 sampling points which included three vertical levels across nine equally divided zones of farms. Using this monitoring method, the D. gallinae infestation level can be assessed objectively on caged-layer poultry farms. Moreover, the method is more sensitive than the conventional method in detecting very small populations of mites. Conclusions: This method can be used to identify the initial stages of D. gallinae infestation in the caged-layer poultry farms, and therefore, will contribute to establishment of effective control strategies for this mite.

ORiginal Article : A Multicenter Phase II Trial of Gemcitabine Plus Oxaliplatin in Unresectable Gallbladder Cancer

( Sang Myung Woo ),( Sang Hyub Lee ),( Ji Won Yoo ),( Ki Young Yang ),( Jung Gyun Seo ),( Joo Kyung Park ),( Jin Hyeok Hwang ),( Woo Jin Lee ),( Ji Kon Ryu ),( Yong Tae Kim ),( Yong Bum Yoon ) The Editorial Office of Gut and Liver 2013 Gut and Liver Vol.7 No.5

Background/Aims: No standard chemotherapy has been established for advanced gallbladder cancer. The authors studied the activity and tolerability of a gemcitabine and ox-aliplatin (GEMOX) combination in unresectable gallbladder cancer (GBC). Methods: Adult patients with pathologically confirmed unresectable GBC were prospectively recruited at three centers. No patient had received prior chemotherapy or radiotherapy. Patients received cycles of gemcitabine at 1,000 mg/m2 on day 1, followed by oxaliplatin at 100 mg/m2 on day 2, every 2 weeks. The primary study endpoint was time to progression. Results: Forty patients with unresect-able GBC were enrolled. The median age was 60 years (range, 38 to 79 years). All patients showed good performance status. Of the 33 analyzable patients, 12 achieved partial re-sponse (36%), 17 stable disease (52%), and four progressive disease (12%). No patient achieved a complete response. The tumor control rate was 88%. At a median follow-up of 6.8 months, the median time to progression was 5.3 months (95% confidence interval [CI], 3.7 to 6.9), and median overall survival was 6.8 months (95% CI, 6.1 to 7.5). Nine of the 40 patients (23%) experienced at least a grade-3 adverse event, but no patient experienced a grade-4 adverse event. Conclu-sions: GEMOX combination therapy is a feasible option and is well tolerated in unresectable GBC. (Gut Liver 2013;7:594-598)

Research Articles : Agricultural Process and Food Engineering ; A Melon Fruit Grading Machine Using a Miniature VIS/NIR Spectrometer: 2. Design Factors for Optimal Interactance Measurement Setup

( Sang Ryong Suh ),( Kyeong Hwan Lee ),( Seung Hwa Yu ),( Hwa Sun Shin ),( Soo Nam Yoo ),( Young Soo Choi ) 한국농업기계학회 2012 바이오시스템공학 Vol.37 No.3

Purpose: In near infrared spectroscopy, interactance configuration of a light source and a spectrometer probe can provide more information regarding fruit internal attributes, compared to reflectance and transmittance configuration. However, there is no through study on the parameters of interactance measurement setup. The objective of this study was to investigate the effect of the parameters on the estimation of soluble solids content (SSC) and firmness of muskmelons. Methods: Melon samples were taken from greenhouses at three different harvesting seasons. The prediction models were developed at three distances of 2, 5, and 8 cm between the light source and the spectrometer probe, three measurement points of 2, 3, and 6 evenly distributed on each sample, and different number of fruit samples for calibration models. The performance of the models was compared. Results: In the test at the three distances, the best results were found at a 5 cm distance. The coefficient of determination (Rcv 2) values of the cross-validation were 0.717 (standard error of prediction, SEP=1.16 °Brix) and 0.504 (SEP=4.31 N) for the estimation of SSC and firmness, respectively. The minimum measurement point required to fully represent the spectral characteristics of each fruit sample was 3. The highest Rcv 2 values were 0.736 (SEP=0.87 °Brix) and 0.644 (SEP=4.16 N) for the estimation of SSC and firmness, respectively. The performance of the models began to be saturated when 60 fruit samples were used for developing calibration models. The highest Rcv 2 of 0.713 (SEP=0.88 °Brix) and 0.750 (SEP=3.30 N) for the estimation of SSC and firmness, respectively, were achieved. Conclusions: The performance of the prediction models was quite different according to the condition of interactance measurement setup. In designing a fruit grading machine with interactance configuration, the parameters for interactance measurement setup should be chosen carefully.

Development of an activated carbon filter to remove NO₂ and HONO in indoor air

Yoo, Jun Young,Park, Chan Jung,Kim, Ki Yeong,Son, Youn-Suk,Kang, Choong-Min,Wolfson, Jack M.,Jung, In-Ha,Lee, Sung-Joo,Koutrakis, Petros Elsevier 2015 Journal of hazardous materials Vol.289 No.-

<P><B>Abstract</B></P> <P>To obtain the optimum removal efficiency of NO<SUB>2</SUB> and HONO by coated activated carbon (ACs), the influencing factors, including the loading rate, metal and non-metal precursors, and mixture ratios, were investigated. The NO<I> <SUB>x</SUB> </I> removal efficiency (RE) for K, with the same loading (1.0wt.%), was generally higher than for those loaded with Cu or Mn. The RE of NO<SUB>2</SUB> was also higher when KOH was used as the K precursor, compared to other K precursors (KI, KNO<SUB>3</SUB>, and KMnO<SUB>4</SUB>). In addition, the REs by the ACs loaded with K were approximately 38–55% higher than those by uncoated ACs. Overall, the REs (above 95%) of HONO and NO<I> <SUB>x</SUB> </I> with 3% KOH were the highest of the coated AC filters that were tested. Additionally, the REs of NO<I> <SUB>x</SUB> </I> and HONO using a mixing ratio of 6 (2.5% PABA (<I>p</I>-aminobenzoic acid)+6% H<SUB>3</SUB>PO<SUB>4</SUB>):4 (3% KOH) were the highest of all the coatings tested (both metal and non-metal). The results of this study show that AC loaded with various coatings has the potential to effectively reduce NO<SUB>2</SUB> and HONO levels in indoor air.</P> <P><B>Highlights</B></P> <P> <UL> <LI> This study developed an activated carbon to simultaneously remove both NO<SUB>2</SUB> and HONO. </LI> <LI> We investigated various factors (loading rate, metal precursors, and mixture ratios). </LI> <LI> NO<SUB>2</SUB> and HONO could be efficiently removed by an improved activated carbon filter that was impregnated with two types of compounds (2.5% PABA+6% H<SUB>3</SUB>PO<SUB>4</SUB> and 3% KOH). </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>