호밀 (Secale cereale L.)유식물의 질산환원효소의 활량과 단백질함량에 관한 Cycloheximide의 영향

권오용,이영하,오희목,박흥순,이진철 충남대학교 자연과학연구소 1988 忠南科學硏究誌 Vol.15 No.2

This work was accomplished with 6 day-old rye (Secale cereale L.) seedlings to determine the effect of the antibiotics by cycloheximide on in vivo nitrate reductase activity, protein and RNA content. The nitrate reductase activity by treating 0.1μM and 0.1 mM cycloheximide was lower than that of the control group. The optimum pH for the nitrate reductase of the control group was 7.6 in the range of pH 5.6-8.0, but, in the case of 0.1mM cycloheximide treatment group, the optimum pH was decreased to 6.8 within the weak the acid region. The protein content of shoot and root in the 0.1μM and 0.1 mM cycloheximide treatment groups were respectively found at the 96%, 82% level of that in the control group. In the higher cycloheximide treatment (0.1 mM CH), not only nitrate reductase activity and protein content but also RNA content was greatly decreased. In this fact, it was suggested that the 0.1 mM cycloheximide had affected on the RNA polymerase. On the basis of there results, it was concluded that the decreased nitrate reductase activity by treating cycloheximide occurred primarily according to the decreased nitrate uptake, and the decreased protein and RNA content depended upon the results of the direct and indirect effect of cycloheximide on the protein synthesis.

금강유역 수질오염 실태조사

최신석,오희목,강명규,조통래,이규승,유종국,이영하 忠南大學校 環境問題硏究所 1988 環境硏究 Vol.6 No.1

조사 구역 상류 지점의 평균 수질은 pH가 7.1, DO가 8.3mg/ℓ, BOD가 1.7mg/ℓ, COD가 1.7mg/ℓ, SS가 15.4mg/ℓ, 분변성 대장균이 1.1x10³ MPN/100ml고 상수원 수 1급 내지 2급의 양호한 상태이고 하류지점의 수질은 pH가 6.8, DO가 7.7mg/ℓ, BOD가 2.2mg/ℓ, COD가 2.4mg/ℓ, 분변성 대장균이 1.7x10³MPN/100ml로 상수원 수 2급 내지 3급의 상태를 나타내 하류로 내려가면서 오염도가 증가하는 것으로 나타났다. 청주시의 영향을 크게 받는 미호천 하류의 평균수질은 pH가 7.2, DO가 6.3mg/ℓ, BOD가 16.2mg/ℓ, COD가 15.1mg/ℓ, 분변성 대장균이 1.6x10??MPN/100ml로 심각한 오염 상태를 보여 청주시와 대전시가 금강 본류의 주 수질 오염원으로 나타났다. 영양 염류 (NH₃-N, NO₂-N, NO₃-N, PO₄-P)와 무기성 유해 성분 (Cl-, CN-, F-)의 함량은 지천의 일부 지점을 제외하고는 극히 낮은 수준으로 검출되었으며, 유기인, 페놀류, PCB 성분은 조사기간 중 전혀 검출되지 않았다. 또한 Fe, Zn, Cu, Mn, Cr, Pb, Cd, Hg등의 중금속은 검출되지 않거나 하천 수질 기준 또는 식용수 수질 기준의 허용치를 초과하지 않는 양호한 수준으로 나타났다.

가와사끼병 환아의 혈청 프로테옴 지도 비교

백승연,이영목,이광훈,김동수 대한소아과학회 2004 小兒科 Vol.47 No.1

Myocardial atrophy in children with mitochondrial disease and Duchenne muscular dystrophy

Lee, Tae Ho,Eun, Lucy Youngmin,Choi, Jae Young,Kwon, Hye Eun,Lee, Young-Mock,Kim, Heung Dong,Kang, Seong-Woong The Korean Pediatric Society 2014 Clinical and Experimental Pediatrics (CEP) Vol.57 No.5

Purpose: Mitochondrial disease (MD) and Duchenne muscular dystrophy (DMD) are often associated with cardiomyopathy, but the myocardial variability has not been isolated to a specific characteristic. We evaluated the left ventricular (LV) mass by echocardiography to identify the general distribution and functional changes of the myocardium in patients with MD or DMD. Methods: We retrospectively evaluated the echocardiographic data of 90 children with MD and 42 with DMD. Using two-dimensional echocardiography, including time-motion (M) mode and Doppler measurements, we estimated the LV mass, ratio of early to late mitral filling velocities (E/A), ratio of early mitral filling velocity to early diastolic mitral annular velocity (E/Ea), stroke volume, and cardiac output. A "z score" was generated using the lambda-mu-sigma method to standardize the LV mass with respect to body size. Results: The LV mass-for-height z scores were significantly below normal in children with MD ($-1.02{\pm}1.52$, P<0.001) or DMD ($-0.82{\pm}1.61$, P =0.002), as were the LV mass-for-lean body-mass z scores. The body mass index (BMI)-for-age z scores were far below normal and were directly proportional to the LV mass-for-height z scores in both patients with MD (R =0.377, P<0.001) and those with DMD (R =0.330, P=0.033). The LV mass-for-height z score correlated positively with the stroke volume index (R =0.462, P<0.001) and cardiac index (R =0.358, P<0.001). Conclusion: LV myocardial atrophy is present in patients with MD and those with DMD and may be closely associated with low BMI. The insufficient LV mass for body size might indicate deterioration of systolic function in these patients.

Dihydroceramide is a key metabolite that regulates autophagy and promotes fibrosis in hepatic steatosis model

Lee, Ah Young,Lee, Jae Won,Kim, Ji-Eun,Mock, Hyuck Jun,Park, Sungjin,Kim, Sanghwa,Hong, Seong-Ho,Kim, Ji-Young,Park, Eun-Jung,Kang, Kyung-Sun,Kim, Kwang Pyo,Cho, Myung-Haing Academic Press 2017 Biochemical and biophysical research communication Vol. No.

<P><B>Abstract</B></P> <P>Non-alcoholic fatty liver disease (NAFLD) is an increasingly common chronic liver disease worldwide. Sphingolipids are a family of lipids that play essential roles as critical regulators in metabolic disorders. Some sphingolipids are known key factors in metabolic dysfunction. However, the precise effect of dihydroceramide on NAFLD remains unknown. Here, we report how dihydroceramide in autophagosome accumulation activates fibrogenesis in human liver Chang cells treated with free fatty acids (FFA). According to LC/MS lipid profiling, FFA increased the levels of sphingolipids and triacylglycerol (TG). To demonstrate the potential role of dihydroceramide metabolism in autophagy, several sphingolipid synthesis inhibitors were used. Increased dihydroceramide led to impairment of autophagic flux, resulting in increased TG storage in lipid droplets (LD) and upregulated expression of fibrosis markers. Hepatic stellate cells (HSCs, LX-2 cells) were co-cultured with Chang cells to assess the potential fibrogenic response to dihydroceramide, Treatment with rapamycin recovered autophagic flux in Chang cells and fibrogenesis in the co-culture system. Our results identified a critical function of dihydroceramide metabolism in autophagy. It could play an important role in the progression of NAFLD associated with lipid over-accumulation. Therefore, preventing autophagic flux by regulating dihydroceramide could be a potential strategic approach for providing therapy for NAFLD.</P> <P><B>Highlights</B></P> <P> <UL> <LI> FFA uptake increases sphingolipids in liver cells. </LI> <LI> Dihydroceramide product correlates with autophagosome accumulation. </LI> <LI> Dihydroceramide impairs autophagic flux and increases lipid droplets. </LI> <LI> Rapamycin recuperates fibrosis by recovering autophagic flux by increased dihydroceramide. </LI> </UL> </P>

소설, 공간, 정치 -모하메드 딥의 "알제리 3부작"의 공간 묘사-

( Young Mock Lee ) 서울대학교 인문학연구원 2013 人文論叢 Vol.69 No.-

정체성, 기억, 역사: 아르키와 그 자녀들의 경우

이영목 ( Young Mock Lee ) 한국불어불문학회 2006 불어불문학연구 Vol.0 No.66

암세포 Bcl-2 family 유전자 군의 DNA 메틸화 연구

강영섭 ( Young Suep Kang ),이선영 ( Sun Young Lee ),정상근 ( Sang Gun Jung ),한지유 ( Ji You Han ),고정재 ( Jeong Jae Ko ),배지현 ( Jee Hyeon Bae ),나영정 ( Young Junh Na ),이찬 ( Chan Lee ),목정은 ( Jung Un Mock ),김승조 ( Sung 대한산부인과학회 2007 Obstetrics & Gynecology Science Vol.50 No.7

목적: 본 연구는 암세포주와 난소암 조직에서 세포예정사의 핵심 조절 단백질인 Bcl-2 family 유전자의 DNA 메틸화 여부를 암과의 관련성을 밝히는데 그 목적이 있다. 연구 방법: 자궁경부암 세포주인 HeLa, CaSki 그리고 만성골수성 혈액암 세포주인 K562 세포에서 genomic DNA를 추출하여 sodium bisulfite를 통한 cytosine 염기를 uracil로 치환하였다. 치환된 염기서열은 methylation과 unmethylation을 특이적으로 확인할 수 있는 primer를 이용하여 MSP (Methylation Specific PCR)을 수행한 후 암세포에서 Bcl-2 family 유전자의 DNA 메틸화 여부를 판별하였다. 결과: 각각의 세포주에서 antiapoptotic Bcl-2 family 유전자 군인 Mcl-1과 Bcl-2 유전자는 DNA 메틸화가 이루어지지 않은 것으로 관찰되었으며, proapoptotic Bcl-2 family 구성원인 Harakiri 유전자는 DNA 메틸화가 이루어진 것을 확인하였다. 반면, 다른 proapoptotic Bcl-2 family 유전자인 Noxa 유전자는 DNA 메틸화가 이루어지지 않은 것으로 관찰되었다. 또한 난소암 조직에서의 DNA 메틸화 여부를 살펴본 결과 Mcl-1과 Noxa는 세포주에서와 같은 결과를 보였고, Harakiri 유전자의 경우 hypomethylation으로 관찰되었다. 결론: 본 연구는 암세포주와 난소암 조직에서 proapoptotic Bcl-2 family 유전자인 Harakiri와 Noxa의 경우, 유전자 특이적인 DNA의 메틸화가 나타났으며 antiapoptotic 유전자는 DNA의 메틸화가 나타나지 않았다. 이로써 Bcl-2 유전자군과 암세포의 세포사멸을 억제하는 기작을 DNA의 메틸화를 통한 특이 유전자 발현 억제에 의해서 이루어질 수 있다고 사료된다. Objective: Promoter methylation of Bcl-2 family genes in cancer cells were studied to verify possible correlation between DNA methylation pattern of Bcl-2 family members and cancer. Methods: The genomic DNAs were extracted from different cancer cell lines, HeLa, CaSki and K562, and ovarian cancer tissue from patients. The cytosine residues were converted to uracil by sodium bisulfite treatment. MSP (methylation specific PCR) was performed to determine the methylation status of Bcl-2, Mcl-1, Noxa, and Harakiri promoters. Using primers that distinguish methylated DNA from unmethylated DNA after bisulfite modification of DNA, MSP was conducted to observe the methylation pattern of Bcl-2 family genes in different cancer cells. Results: The promoter regions of Bcl-2 family genes including Mcl-1, Bcl-2, and Noxa were not methylated in cancer cells, whereas the proapoptotic Bcl-2 family gene Harakiri was detected as methylated in the cancer cell lines and hypomethylated in the ovarian cancer tissue. Conclusion: The present study demonstrated the differential methylation profiles of Bcl-2 family genes in cancerous cells, which suggests a possible connection between the methylation pattern of some of Bcl-2 family genes and ovarian cancer.

정치적 정체성과 상상력 -알베르 메미와 아쉴 음벰베를 중심으로-

이영목 ( Young Mock Lee ) 서울대학교 인문학연구원 2011 人文論叢 Vol.65 No.-

Le propos de cette etude consistait a etablir des rapports entre l`identite politique et l`imagination politique dans les textes principaux des deux representants respectifs de la pensee coloniale et de la pensee postcoloniale: Albert Memmi et Achille Mbembe. Nous avons suppose que les differences de deux pensees - role determinant des facteurs socio-economiques dans le systeme colonial dans la pensee coloniale et mise en valeur du culturel dans la pensee postcoloniale - produiraient les specificites des deux discours. Mais une fois constatee la primaute du culturel communement admise dans ces deux auteurs, nous sommes amene plutot a nous interesser a la facture meme de leurs discours qu`aux contenus conceptuels et ideels de ceux-ci. Nous avons degage chez Memmi une ?logique implacable de la necessite coloniale? et chez Mbembe une ?pensee de l`enchevetrement et de la concatenation? qui consiste a l`approchement graduel au fait a travers un mouvement continu de mises au point successives. Ces demarches de la pensee representent leurs efforts intellectuels qui visent a une elaboration d`un savoir inedit et original a l`ecart de, et par rapport a, l`universalisme rationnel de l`Occident remis en cause.

18 세기 프랑스 유물론의 인간관과 정치 사상 - 자연법 개념을 중심으로

이영목(Young Mock Lee) 한국불어불문학회 2002 불어불문학연구 Vol.50 No.-

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