Comparison of physical properties of Steamed Rice Cake (Jeung-pyun) using Dry-milled Rice Flour, a variety of yeast and homogenizer

Young-Lan Son,Sa-Ra Park,Hee-Sun Kim,Gwi-Jung Han 한국식생활문화학회 2017 한국식생활문화학회학술대회논문집 Vol.2017 No.05

원외처방전 검토시 발생한 문의처방 분석

허란희,이주연,임영근,조윤숙,이지영,이영희,정성훈,박종윤,소원희,진보영,한현주,이병구,손인자 한국병원약사회 2002 병원약사회지 Vol.19 No.1

The purpose of this study is to evaluate hospital pharmacist's double-check task of outpatient prescription since separation of dispensary from medical practice carried out, which is being shifted to pharmacists out of hospital gradually. We analyzed 2,613 conference call on prescription out of one-year 488,481 outpatient prescription made in SNUH(Seoul National University Hospital). And it is classified and analyzed by month, by department and by conference call item. This study can provide useful information for doctors and pharmacists. Consequently, the doctors can avoid prescription errors. And the pharmacists can promote the capacity of double-checking prescriptions.

담금 방법을 달리한 전통 삼해주의 발효 중 이화학적 특성 변화

임채란(Chae-Lan Lim),손희진(Hee-Jin Son),홍은정(Eun Jeung Hong),한기영(Kee Young Han),최진영(Jin Young Choi),조인영(In-Young Cho),김계원(Gye-Won Kim),노봉수(Bong-Soo Noh) 한국식품과학회 2009 한국식품과학회지 Vol.41 No.2

민속주의 우수성을 과학적으로 입증함과 함께 전통 반가주의 복원 및 공장 생산의 가능성을 열기 위하여 발효 온도를 상승시킴으로써 전통적인 발효 방법을 변형해 발효 시간을 단축하여 2가지 방법으로 삼해주를 제조하였다. 3번의 담금을 기준으로 삼해주의 발효 기간에 따라 이화학적 성질을 분석하였는데, 2단과 3단 담금 직후 pH 값은 상승 하였으며, 총산 함량은 감소하였다. 아미노태 질소 함량은 발효 초 1.31 mg%로 낮았으나 발효가 진행됨에 따라 증가함을 보였고, 덧술이 첨가된 직후에는 낮은 함량을 나타내었다. 이는 덧술 첨가에 의한 시료의 희석 효과로 여겨진다. 삼해주의 대표적인 유기산으로는 lactic acid, succinic acid로 나타났고, citric acid, acetic acid, propionic acid도 일부 검출되었다. 삼해주의 대표적인 유리당으로는 glucose, maltose, lactose로 확인되었고, fructose, sucrose도 소량 검출 되었다. 색도의 경우 덧술 첨가 직후 명도를 나타내는 L 값이 감소하였으며, 발효가 완료되었을 때 a값이 가장 낮고, b 값은 가장 높았다. 전자코로 휘발성분을 분석한 결과 삼해주의 향에 가장 큰 영향을 주는 것은 알코올로 나타났으며, 발효가 진행됨에 따라 제 1주성분 값이 음(?)에서 양(+)의 값으로 이동을 하였고, 제 1주성분이 9.00의 값을 나타내었을 경우 삼해주의 품질을 유지하는 것으로 나타났다. Samhaeju is a traditional Korean noble rice wine, in which its processing is performed at low temperatures for an extended fermentation time and with three brewing steps. In this study, Samhaeju was prepared by different brewing methods that were modified from the method in the literature. Chemical composition of samples were determined to evaluate the quality of the Samhaeju. The Samhaeju was analyzed for pH, total acids, amino nitrogen, Hunter color values, free sugars, organic acids, and volatile components. Before the addition of the second and third mashing, pH values had decreased slightly and total acids had rapidly increased. Free sugar and amino nitrogen contents were high in final product. After addition of the third mashing as the advanced step, a dilution effect was shown. Glucose (A: 0.77-7.0%, B: 0.77-3.81%) was a major free sugar, and lactic acid (A: 0-2,840mg%, B: 0-3,375mg%) was a major organic acid during the entire period of fermentation. Based on principal component analysis of electronic nose data for the components, the stages of Samhaeju fermentation were primarily separated along the first principal component (PC, proportion : 98.67%). The first PC component (PC1) was moved from negative value(-6.16) to positive value(9.00) with increasing fermentation time. The change patterns for pH and total acid during the fermentation period were similar to those of PC1 from the data obtained by electronic nose based on mass spectrometry.

삼해주와 시판 곡주의 생리 기능성 및 세포 독성 효과

임채란(Chae-Lan Lim),손희진(Hee-Jin Son),조인영(In-Young Cho),김계원(Gye-Won Kim),최수진(Soo-Jin Choi),김인선(In-Sun Kim),한기영(Kee Young Han),최진영(Jin Young Choi),노봉수(Bong Soo Noh) 한국식품과학회 2009 한국식품과학회지 Vol.41 No.6

잊혀져가고 있는 전통주의 우수함을 알리고자 한국의 전통 반가주인 삼해주를 제조하고 시판되고 있는 곡주와 비교 실험하며, 생리활성 및 세포독성에 관한 연구를 진행하였다. 기존의 문헌에 소개된 방법을 변형하여 3가지 종류의 삼해주를 제조하였으며, 시중에서 곡주 5종을 구입하여 실험하였다. DPPH 라디칼 제거능과 아질산염 소거능은 곡주 G가 가장 뛰어났으며, ABTS 라디칼 제거능은 삼해주가 가장 우수하였다. 삼해주 중에서는 C 시료가 DPPH 라디칼 제거능, 아질산염 소거능, ABTS 라디칼 소거능이 가장 뛰어났다. 혈전용해능의 경우 삼해주 A-C가 13-17U로 다른 곡주에 비해 그 활성이 높게 나타났다. 각 시료에 대하여 10-160배까지 단계적으로 희석하여 HeLa, A549, L-132 세포에 처리하였을 때, 인체유래 자궁암 세포주인 HeLa 세포의 경우 10배 희석액에서 삼해주가 강한 세포 독성을 보였고, A549 세포의 경우 10배 희석액에서 삼해주의 세포독성 효과가 크나 시료에 따라 그 편차가 심하였다. L-132의 경우 10배 희석액에서 삼해주의 세포독성 효과가 다른 시료에 비해 강하였으나, 다른 암세포에 비해 약한 것을 알 수 있다. 본 연구의 결과에 의하면 삼해주에 존재하는 미지의 약리 성분이 생리 활성 및 암세포의 성장에 영향을 미치는 것으로 판단되었다. 이는 삼해주 제조 시 사용된 누룩과 오랜 발효 기간에 의한 영향으로 생각되며, 누룩에 대한 연구가 더 진행되어야 할 것으로 여겨진다. This study was conducted to investigate the antioxidant activity, fibrinolytic activity and cytotoxic effect of Korean traditional noble rice wine made using different methods (A-C) and commercial rice wine (D-H) on various tumor cell lines. The antioxidant activity of rice wine was measured by DPPH (2,2-dipicryl-1-picrylhydrazyl), ABTS [2,2'-azinobis(3-ethylbenzthiazoline-6-sulphonic acid)] and NO (nitric oxide) radical scavenging assay. In this study, Samhaeju showed the greatest fibrinolytic activity of 13-17U and exhibited the highest antioxidant activity. Among the different Samhaeju, the sample prepared using method C had the highest antioxidant activity. The cytotoxic effect of rice wine were also examined against the human cancer cell line (A549 cells and HeLa cells) based on the results of a WST-1 assay and morphological changes. Rice wine induced the inhibition of cell proliferation and morphological changes in tumor cell lines in a concentration-dependent manner, with Samhaeju diluted 10 fold having the strongest effect on these factors. These findings suggest that Korean rice wine has antioxidant activity and cytotoxic effect, and that these factors are influenced by the method of preparation.

Myeloid cell leukemia-1 regulates the cell growth and predicts prognosis in gastric cancer

LEE, WAN-SIK,PARK, YOUNG-LAN,KIM, NURI,OH, HYUNG-HOON,SON, DONG-JUN,KIM, MI-YOUNG,OAK, CHAN-YOUNG,CHUNG, CHO-YUN,PARK, HYUNG-CHUL,KIM, JONG-SUN,MYUNG, DAE-SEONG,CHO, SUNG-BUM,JOO, YOUNG-EUN Spandidos Publications 2015 International journal of oncology Vol.46 No.5

<P>The expression of myeloid cell leukemia-1 (Mcl?1), a member of the anti-apoptotic Bcl-2 protein family, has been associated with tumor progression and adverse patient outcome. The aims of current study were to evaluate whether Mcl-1 affects the survival or death of gastric cancer cells, and to investigate the prognostic value of its expression in gastric cancer. PcDNA3.1-Mcl-1 expression and Mcl-1 siRNA vectors were used to overexpress and silence Mcl-1 expression in gastric cancer cell lines including SNU638 and TMK1, respectively. Immunohistochemistry was used to determine the expression of Mcl-1 in gastric cancer tissues. Apoptosis was determined by the TUNEL assay, and cell proliferation was determined by immunostaining with a Ki-67 antibody. Mcl-1 knockdown induced apoptosis through the upregulation of caspase-3, and -7, and PARP activity, and the release of Smac/DIABLO and Omi/HtrA2 into the cytoplasm. Additionally, cell cycle arrest occurred due to decrease of cyclin D1, cell division cycle gene 2 (cdc2), and cyclin-dependent kinase 4 and 6. In contrast, overexpression of Mcl-1 inhibited apoptosis and cell cycle arrest. Mcl-1 knockdown did not suppress tumor cell proliferation in gastric cancer cells, whereas overexpression of Mcl-1 enhanced tumor cell proliferation. The JAK2 and STAT3 signaling cascades were significantly blocked by Mcl-1 knockdown. The mean Ki-67 labeling index (KI) value of Mcl-1 positive tumors was significantly lower than that of Mcl-1 negative tumors. However, there was no significant difference between Mcl-1 expression and the apoptotic index (AI). Mcl-1 expression was significantly increased in gastric cancer tissues compared to normal gastric mucosa tissues, and was associated with age, tumor size, stage, depth of invasion, lymph node metastasis and poor survival. Our study showed that Mcl-1 regulates the cell growth and might be a potential prognostic marker for gastric cancer.</P>

차나무의 품종, 부위, 채취시기에 따른 향기생성 효소의 활성과 향기배당체의 함량

정재천(Jae-Cheon Jeong),최정연(Jeong-Youn Choi),최문희(Moon-Hui Choi),손영란(Young-Lan Son),조정용(Jeong-Yong Cho),김선재(Seon-Jae Kim),문제학(Jae-Hak Moon),박근형(Keun-Hyung Park),마승진(Seung-Jin Ma) 한국차학회 2012 한국차학회지 Vol.18 No.4

Enzymatic activities and amounts of aromatic precursors, which are related with aroma formation in fermanted tea, were indirectly measured in vanious cultivars (Korean native cultivar, Taicha No.12, Yabukita), harvest time (May, July, September), and parts (aged leaves, young leaves, stem) by means of crude enzyme assay. Enzyme activities related to aroma formation in stems and young leaves were higher than those in aged leaves. Activities in parts harvested in September and July were higher than in those harvested in May. Total amounts of aromatic precursors were higher in young leaves than those in aged leaves from each cultivar. Amounts of precursors of geraniol and methyl salicylate were much higher in each part of Taicha No.12 than those of Yabukita, although much greater amounts of benzyl alcohol and 2-phenylethanol were liberated from Yabukita.

The dual roles of c-Jun NH2-terminal kinase signaling in Cr(VI)-induced apoptosis in JB6 cells.

Son, Young-Ok,Hitron, John Andrew,Cheng, Senping,Budhraja, Amit,Zhang, Zhuo,Lan Guo, Nancy,Lee, Jeong-Chae,Shi, Xianglin Academic Press 2011 TOXICOLOGICAL SCIENCES Vol.119 No.2

<P>Occupational exposure to chromium (Cr) compounds has been shown to cause serious toxic and carcinogenic effects. The skin is an important target for the compounds in industrially exposed Cr workers. c-Jun NH(2)-terminal kinase (JNK) regulates cell proliferation, apoptosis, and differentiation. This protein's effects on cellular response depend upon the cell type and stimuli. The mechanisms by which hexavalent chromium (Cr(VI)) leads to apoptosis in the skin are unclear at present. The aim of this study is to examine whether JNK regulates apoptosis in Cr(VI)-exposed mouse JB6 epidermal cells. The present study showed that Cr(VI) induced apoptotic cell death through JNK activation. The blockage of JNK by small interference RNA (si-RNA) transfection suppressed Cr(VI)-induced apoptotic cell death with the concomitant downregulation of antiapoptotic Bcl-2 family proteins, mitochondrial membrane depolarization (δψm), caspase activation, and poly (ADP-ribose) polymerase cleavage. However, inhibition of c-Jun expression by si-RNA transfection enhanced cytotoxicity, which corresponded to increasing apoptosis and δψm. This phenomenon is associated with p53 activation caused by increasing reactive oxygen species (ROS) levels because of the downregulation of superoxide dismutase expression in si-c-Jun-transfected cells. Taken together, Cr(VI) induces apoptosis via JNK-mediated signaling, whereas c-Jun activation acts as an inhibitor of apoptotic signaling. Additionally, ROS generated by Cr(VI) is a pivotal regulator of JNK.</P>

협동학습 전략이 중학교 생물학습에서 학생들의 학업성취도와 과학에 대한 태도에 미치는 영향

정영란,손대희 한국과학교육학회 2000 한국과학교육학회지 Vol.20 No.4

과학교육에 있어서 전통적 수업은 다양한 학생들의 특성과 요구를 수용하지 못하여 학생들의 과학에 대한 흥미를 저하시켰다. 평균적인 다수의 학생들을 대상으로 한 수업에서 결국 많은 학생들이 수업에 흥미를 잃게 되고 학생들은 타율적인 대상으로만 머물러 자신의 능력을 발휘할 기회를 얻지 못하고 있다. 이러한 문제를 극복하고 학생들의 활동을 격려하며 구성원들의 합의를 통한 문제해결을 이끌어내기 위한 수업 방식으로서 협동학습이 제안되었다. 본 연구에서는 생물교과에 협동학습을 적용하여 그 효과를 분석하고 소집단 학습과 협동학습의 차이를 알아보았다. 중학교 2학년 「동물의 구조와 기능」단편에서 협동학습, 소집단 학습, 전통적학습을 실시하여 학생들의 과학 학업 성취도와 과학학습 태도에 대한 효과를 분석하였다. 연구대상은 서울시 소재 남녀공학 중학교 2학년 188명이며 10주간 10차시 수업처치를 하였고 사전-사후 통제집단 설계를 사용하였다. 연구 결과는 SPSS통계 프로그램을 이용하여 공변량분석으로 분석하였다. 연구 결과 협동학습은 소집단학습이나 전통적 수업에 비해 학생들의 학업 성취도와 과학학습 태도에서 유의미한 효과가 있는 것으로 나타났다(p<.05). 그리고 협동학습은 학업 성취도면에서 성취수준이 상위인 학생들에게 효과가 없었고 중위와 하위의 학생들에게는 유의미한 효과가 있었다 (p<.05). 또한 협동학습은 남학생과 여학생 모두에게 효과적이었다(p<.05). 협동학습은 과학학습 태도의 세 영역인 감정적 영역, 행동 의도적 영역, 인지적 영역에서 모두 유의미한 효과를 보였다(p<.01). The cooperative learning movement began as parts of the desegregation process in America, aiming at increasing academic achievement and social skills among diverse students. Cooperative learning may be defined as a classroom learning environment in which students work together in small heterogeneous groups. Although many studies have shown the effectiveness of cooperative learning in a variety of subjects, relatively few have focused on biology. In this study, we investigated the effects of cooperative learning on students' achievement and attitude of middle school biology students. For this purpose this study compared three sections. In one section, a cooperative learning strategy was used. Second section was taught in small groups and the third section was instructed in the traditional method. The unit 'Structures and functions of animals' was used. A total of 188 students were included in this study. These classes were treated for 10hours during 10weeks from September 1 to November 28, 1999. The pretests-posttests control group design was applyed. An analysis of covariance(ANCOVA) was used as the data analysis procedure. Significant differences were found in the achievement and the attitude of students using cooperative learning strategy(p<.05) when compared to traditional classroom structure and small group learning. Cooperative learning was more effective in the low-ability and average-ability students than the high-ability students in the science achievement. Cooperative learning is effective in both male and female students. And students in the cooperative group achieved better than those in other groups in affective, behavioral, and intention-cognitive domain of science attitude.

경량성형재를 이용한 중공슬래브의 구조성능

손민서(Son, Min-Seo),김병전(Kim, Byoung-Jeon),윤성호(Youn, Sung-Ho),노영곤(Ro, Young-Gon),이상현(Lee, Sang-Hyun),정란(Chung, Lan) 대한건축학회 2010 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.30 No.1(구조계)

뇌졸중 환자의 회복에 미치는 원예치료의 효과

손기철(Son Ki-Cheol),김은경(Kim Eun-Kyoung),나영무(Na Young-Moo),이중헌(Lee Joong-Hun),하영란(Ha Young-Lan),이선실(Lee Sun-Sil) 한국원예학회 1999 원예과학기술지 Vol.17 No.5