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RISS 인기검색어
- 검색키워드
- 저자 : Yihui Chen (검색결과 13 건)
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Yihui Chen,Min Zhang,Wentao Liu,Guoying Li 한국유변학회 2011 Korea-Australia rheology journal Vol.23 No.1
The effect of N-hydroxysuccinimide activated adipic acid (NHS-AA) on the properties of alkali-solubilized collagen solutions was examined. The residual amino group content in crosslinked collagen, determined by trinitrobenzensulfonic acid (TNBS) assay, was decreased with increasing NHS-AA concentration. The results from differential scanning calorimeter (DSC) indicated that the maximum denaturation temperature (Td) of crosslinked collagen solution was about 4.2oC higher than that of un-crosslinked collagen solution (36.6℃). Moreover, the values of storage modulus (G'), loss modulus (G") and complex viscosity (η^*), obtained by means of dynamic frequency sweeps, were increased as NHS-AA concentration added up to 1.5mM, and then decreased slightly when further increased NHS-AA concentration. Besides, for collagen solution crosslinked with 1.5mM NHS-AA, dynamic denaturation temperature (T_(dd)) was about 1.1℃ lower than T_d (40.8℃), and the Arrhenius-type time-temperature superposition (TTS) principle was applied to yield the activation energy to be 474.4kJmol^(-1).
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Chen, Yihui,Zhang, Min,Liu, Wentao,Li, Guoying 한국유변학회 2011 Korea-Australia rheology journal Vol.23 No.1
The effect of N-hydroxysuccinimide activated adipic acid (NHS-AA) on the properties of alkali-solubilized collagen solutions was examined. The residual amino group content in crosslinked collagen, determined by trinitrobenzensulfonic acid (TNBS) assay, was decreased with increasing NHS-AA concentration. The results from differential scanning calorimeter (DSC) indicated that the maximum denaturation temperature ($T_d$) of crosslinked collagen solution was about $4.2^{\circ}C$ higher than that of un-crosslinked collagen solution ($36.6^{\circ}C$). Moreover, the values of storage modulus (G'), loss modulus (G") and complex viscosity (${\eta}^*$), obtained by means of dynamic frequency sweeps, were increased as NHS-AA concentration added up to 1.5 mM, and then decreased slightly when further increased NHS-AA concentration. Besides, for collagen solution crosslinked with 1.5 mM NHS-AA, dynamic denaturation temperature ($T_{dd}$) was about $1.1^{\circ}C$ lower than $T_d$ ($40.8^{\circ}C$), and the Arrhenius-type time-temperature superposition (TTS) principle was applied to yield the activation energy to be $474.4kJmol^{-1}$.
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Chen, Lei,Fang, Bo,Qiao, Liman,Zheng, Yihui The Korean Society for Microbiology and Biotechnol 2022 Journal of microbiology and biotechnology Vol.32 No.6
Esophageal squamous cell carcinoma (ESCC) is the most common primary esophageal malignancy with poor prognosis. Here, due to the necessity for exploring potential therapies against ESCC, we obtained the gene expression data on ESCC from the TCGA and GEO databases. Venn diagram analysis was applied to identify common targets. The protein-protein interaction network was constructed by Cytoscape software, and the hub targets were extracted from the network via cytoHubba. The potential hub nodes as drug targets were found by pharmacophore-based virtual screening and molecular modeling, and the antitumor activity was evaluated through in vitro studies. A total of 364 differentially expressed genes (DEGs) in ESCC were identified. Pathway enrichment analyses suggested that most DEGs were mainly involved in the cell cycle. Three hub targets were retrieved, including CENPF, CCNA2 (cyclin A), and CCNB1 (cyclin B1), which were highly expressed in esophageal cancer and associated with prognosis. Moreover, amentoflavone, a promising drug candidate found by pharmacophore-based virtual screening, showed antiproliferative and proapoptotic effects and induced G1 in esophageal squamous carcinoma cells. Taken together, our findings suggested that amentoflavone could be a potential cell cycle inhibitor targeting cyclin B1, and is therefore expected to serve as a great therapeutic agent for treating esophageal squamous cell carcinoma.
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Treatment of Oily Wastewater with WPO and CWO
Han, Mei,Chen, Yihui,He, Fang,Yu, Li Korean Chemical Society 2014 대한화학회지 Vol.58 No.1
Petroleum refining unavoidably generates large volumes of oily wastewater. The environmentally acceptable disposal of oily wastewater is a current challenge to the petroleum industry. Nowadays, more attentions have been focused on the treatment techniques of oily wastewater. Oily wastewater contained highly concentrated and toxic organic compounds. Wet peroxide oxidation (WPO) and catalytic wet oxidation (CWO) were applied to eliminate pollutants to examine the feasibility of the WPO/CWO of oily wastewater. The results indicated that more than 80% chemical oxygen demand (COD) removal from oily wastewater was achieved with CWO. Homogenous catalyst, $NaHCO_3$ and $Na_2CO_3$ and NaOH showed effective removal for pollutants in oily wastewater. Greater than 90% COD removal was achieved with WPO. It was concluded that WPO was a far more effective process for oily wastewater.
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Wu Pingxian,Chen Dejuan,Wang Kai,Wang Shujie,Liu Yihui,Jiang Anan,Xiao Weihang,Jiang Yanzhi,Zhu Li,Xu Xu,Qiu Xiaotian,Li Xuewei,Tang Guoqing 아세아·태평양축산학회 2023 Animal Bioscience Vol.36 No.1
Objective: Pigs, an ideal biomedical model for human diseases, suffer from about 50% early embryonic and fetal death, a major cause of fertility loss worldwide. However, identifying the causal variant remains a huge challenge. This study aimed to detect single nucleotide polymorphisms (SNPs) and candidate genes for the number of mummified (NM) piglets using the imputed whole-genome sequence (WGS) and validate the potential candidate genes. Methods: The imputed WGS was introduced from genotyping-by-sequencing (GBS) using a multi-breed reference population. We performed genome-wide association studies (GWAS) for NM piglets at birth from a Landrace pig populatiGWAS peak located on SSC11: 0.10 to 7.11 Mbp (Top SNP, SSC11:1,889,658 bp; p = 9.98E-13) was identified in cyclin dependent kinase on. A total of 300 Landrace pigs were genotyped by GBS. The whole-genome variants were imputed, and 4,252,858 SNPs were obtained. Various molecular experiments were conducted to determine how the genes affected NM in pigs. Results: A strong GWAS peak located on SSC11: 0.10 to 7.11 Mbp (Top SNP, SSC11:1,889,658 bp; p = 9.98E-13) was identified in cyclin dependent kinase 8 (CDK8) gene, which plays a crucial role in embryonic retardation and lethality. Based on the molecular experiments, we found that Y-box binding protein 1 (YBX1) was a crucial transcription factor for CDK8, which mediated the effect of CDK8 in the proliferation of porcine ovarian granulosa cells via transforming growth factor beta/small mother against decapentaplegic signaling pathway, and, as a consequence, affected embryo quality, indicating that this pathway may be contributing to mummified fetal in pigs. Conclusion: A powerful imputation-based association study was performed to identify genes associated with NM in pigs. CDK8 was suggested as a functional gene for the proliferation of porcine ovarian granulosa cells, but further studies are required to determine causative mutations and the effect of loci on NM in pigs.
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Rheological properties of fish skin collagen solution: Effects of temperature and concentration
Min Zhang,Yihui Chen,Guoying Li,Zongliang Du 한국유변학회 2010 Korea-Australia rheology journal Vol.22 No.2
To use collagen from freshwater fish skin as an alternative source of mammalian collagens for biomedical applications, we tested the rheological and structural properties of collagen from the skin of largefin longbarbel catfish (Mystus macropterus) by dynamic viscoelasticrheologimenteolFish skin collagen solution (FSCS) exhibited a sheor-thinning fedi behaMysr. The compllastiscosity (h*), loss tangent (tan d) and relaxic visc me of 1.5% FSCS decreased wies tkin ocrease of temperature. Also, tan d decreased wehaMythe relaxic visc me ocreased wies tkin ocrease of FSCS concentration. FSCS tiscconsider coymedicr stocon rhodulus (G'), loss hodulus (G"), h* tested and relaxic visc me and arus) by tan d teste tkaischose of bovine skin collagen solution (BSCS). Hdicver, FSCS behaMed wiesout regularity above 27.5oCi behtiowas in agreiment wies tkinisc me tkat tkindynamic denaturac viscemperature of thed collagen was approx mateoym29.5oC. Thes decreasedindicated tkat temperature and concentration ), ltococenols suitaconcfor isjusting FSCS tiscosity. The Arrhenius-typoce me-temperature superposition (TTS) was appliedCS tiisdition, tkinactitetion energy of 149.6KJ hol-1cfor 1.5% FSCS was calculated accor6KJ hentthe Arrhenius equation, indicatKJ ha weakby dntangliment effect amongal amongal amongal holeculeus (aischat amongal bovine skin collagen molecules, which was in agreement with the results from AFM measurement.
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An Uncertain Graph Method Based on Node Random Response to Preserve Link Privacy of Social Networks
Jun Yan,Jiawang Chen,Yihui Zhou,Zhenqiang Wu,Laifeng Lu 한국인터넷정보학회 2024 KSII Transactions on Internet and Information Syst Vol.18 No.1
In pace with the development of network technology at lightning speed, social networks have been extensively applied in our lives. However, as social networks retain a large number of users’ sensitive information, the openness of this information makes social networks vulnerable to attacks by malicious attackers. To preserve the link privacy of individuals in social networks, an uncertain graph method based on node random response is devised, which satisfies differential privacy while maintaining expected data utility. In this method, to achieve privacy preserving, the random response is applied on nodes to achieve edge modification on an original graph and node differential privacy is introduced to inject uncertainty on the edges. Simultaneously, to keep data utility, a divide and conquer strategy is adopted to decompose the original graph into many sub-graphs and each sub-graph is dealt with separately. In particular, only some larger sub-graphs selected by the exponent mechanism are modified, which further reduces the perturbation to the original graph. The presented method is proven to satisfy differential privacy. The performances of experiments demonstrate that this uncertain graph method can effectively provide a strict privacy guarantee and maintain data utility.
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Zebra-Crossing Automatic Recognition and Early Warning for Intelligent Driving
Mingli Yang,Hua Shi,Yihui Chen,Xiangjin Ran,Chunhui Xu 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.8
Zebra-crossing Recognition is one of the essential parts of the visual based intelligent vehicle navigation or intelligent driving assistant system. In order to address the real-time and robustness, Zebra-crossing Recognition Method which is based on spatial-temporal correlation has been proposed. Firstly, calibrate a camera mounted on the vehicle by a practical method. Then, according to the prior knowledge such from GPS etc, a judgment whether it's in the Crossing area is made. Next, utilize the bipolar property of Zebra-crossing to extract features. Finally, the recognition results are obtained according to the model constraints. In this paper, proposed methods can improve real time identification of the zebra line by using spatial correlation, reduce the cost of recognition and lower errors during identification. The method overcomes some disadvantages of traditional identification approaches based upon video recognition, for instance higher cost and errors.
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