Effect of 7, 8-dihydroxyflavone on the up-regulation of Nrf2-mediated heme oxygenase-1 expression in hamster lung fibroblasts.

Ryu, Min Ju,Kang, Kyoung Ah,Piao, Mei Jing,Kim, Ki Cheon,Zheng, Jian,Yao, Cheng Wen,Cha, Ji Won,Hyun, Chang Lim,Chung, Ha Sook,Park, Jong Cook,Cho, Suk Ju,Hyun, Jin Won Springer 2014 In vitro cellular & developmental biology Animal Vol.50 No.6

<P>The cytoprotective mechanism of 7, 8-dihydroxyflavone (DHF) against oxidative stress-induced cell damage with respect to its stimulatory effect on the expression of heme oxygenase-1 (HO-1), a potent antioxidant enzyme, was investigated in the present study. Up-regulation of HO-1 expression by DHF was both dose and time dependent in lung fibroblast V79-4 cells. DHF also increased the protein expression level of the transcription factor nuclear factor erythroid-2-related factor 2 (Nrf2), and induced the translocation of Nrf2 from the cytosol into the nucleus, leading to elevated HO-1 expression. The siNrf2 RNA-transfection attenuated HO-1 expression induced by DHF treatment. In addition, DHF induced the activation of extracellular signal-regulated kinase (ERK), while U0126 (a specific pharmacological inhibitor of ERK kinase) abrogated DHF-activated Nrf2 and HO-1 expression. This suggests that DHF increased the levels of Nrf2 and HO-1 via ERK-dependent pathways. Furthermore, DHF significantly prevented the reduction of cell viability in response to oxidative stress; however, U0126 attenuated the protective effect of DHF. Taken together, these results demonstrate that DHF protected cells from oxidative stress via the activation of an ERK/Nrf2/HO-1 signaling pathway.</P>

중국유학생들의 학습동기 및 사회적 지지가 대학생활적응에 미치는 영향 - 부산지역을 중심으로 -

( Yao Lan ),주동범 ( Ju Dong-beom ) 한국다문화 디아스포라학회 2018 다문화와 디아스포라연구 Vol.12 No.-

본 연구는 부산지역 소재 대학에 재학하고 있는 중국유학생들을 대상으로 학습동기 및 사회적지지가 대학생활적응에 미치는 영향을 분석하였다. 이를 위해 210명의 표본을 추출하여 중국유학생들의 일반적 특성, 학습동기, 사회적 지지, 대학생활적응을 측정하였으며, 최종 208명이 최종분석에 사용되었다. 주요 결과는 다음과 같다. 첫째, 대학생활적응의 경우 중국유학생들의 일반적 특성을 통제한 후 학습동기 요인에서 자기조절학습전략이 통계적으로 유의한 부적 영향을 미치는 것으로 나타났으며, 사회적지지 요인에서 중국인친구 지지가 통계적으로 유의한 정적 영향을 미치는 것으로 나타났다. 둘째, 학업적응의 경우 중국유학생들의 일반적 특성을 통제한 후 학습동기 요인에서 자기효능감이 통계적으로 유의한 정적 영향을 미치는 것으로 나타났다. 셋째, 사회적응의 경우 중국유학생들의 일반적 특성을 통제한 후 사회적지지 요인에서 중국인친구 지지가 사회적응에 통계적으로 유의한 정적 영향을 미치는 것으로 나타났다. 넷째, 개인정서적응의 경우 중국유학생들의 일반적 특성을 통제한 후 학습동기 요인에서 자기조절학습전략이 통계적으로 유의한 부적 영향을 미치는 것으로 나타났다. 다섯째, 대학환경적응의 경우 중국유학생들의 일반적 특성을 통제한 후 학습동기 요인에서 통제소재가 통계적으로 유의한 정적 영향을 미치는 것으로 나타났으며, 사회적지지 요인에서 중국인친구 지지가 통계적으로 유의한 정적 영향을 미치는 것으로 나타났다. 이러한 주요 결과에 대한 시사점 및 제언이 논의되었다. This study analyzed the impact of learning motivation and social support on college life adjustment using Chinese international students who were attending at university located in Busan. For this, the Chinese international students’ general characteristics, learning motivation, social support, and college life adjustment were measured for a sample of 210 students and 208 students were used for final analysis. Main results were as the followings. First, for college life adjustment, after controlling the Chinese international students’ general characteristics, self-regulation learning strategy in learning motivation factor had statistically significant negative influence and Chinese friend support in social support factor had statistically significant positive influence. Second, for academic adjustment, after controlling the Chinese international students’ general characteristics, self-efficacy in learning motivation factor had statistically significant positive influence. Third, for social adjustment, after controlling the Chinese international students’ general characteristics, Chinese friend support in social support factor had statistically significant positive influence. Fourth, for personal emotion adjustment, after controlling the Chinese international students’ general characteristics, self-regulation learning strategy in learning motivation factor had statistically significant negative influence. Fifth, for college environment adjustment, after controlling the Chinese international students’ general characteristics, locus of control in learning motivation factor had statistically significant positive influence and Chinese friend support in social support factor had statistically significant positive influence. Some implications and suggestions on these main results were discussed.

Live and Dead GFP-Tagged Bacteria Showed Indistinguishable Fluorescence in Caenorhabditis elegans Gut

Ju-Ya Hsiao,Chun-Yao Chen,Mei-Jun Yang,Han-Chen Ho 한국미생물학회 2013 The journal of microbiology Vol.51 No.3

Caenorhabditis elegans has been used for studying hostpathogen interactions since long, and many virulence genes of pathogens have been successfully identified. In several studies, fluorescent pathogens were fed to C. elegans and fluorescence observed in the gut was considered an indicator for bacterial colonization. However, the grinder in the pharynx of these nematodes supposedly crushes the bacterial cells, and the ground material is delivered to the intestine for nutrient absorption. Therefore, it remains unclear whether intact bacteria pass through the grinder and colonize in the intestine. Here we investigated whether the appearance of fluorescence is indicative of intact bacteria in the gut using both fluorescence microscopy and transmission electron microscopy. In wild-type N2 C. elegans, Escherichia coli DH5α, and Vibrio vulnificus 93U204, both of which express the green fluorescence protein, were found intact only proximal to the grinder, while crushed bacterial debris was found in the post-pharyngeal lumen. Nevertheless, the fluorescence was evident throughout the lumen of worm intestines irrespective of whether the bacteria were intact or not. We further investigated the interaction of the bacteria with C. elegans phm-2 mutant, which has a dysfunctional grinder. Both strains of bacteria were found to be intact and accumulated in the pharynx and intestine owing to the defective grinder. The fluorescence intensity of intact bacteria in phm-2 worms was indistinguishable from that of crushed bacterial debris in N2 worms. Therefore, appearance of fluorescence in the C. elegans intestine should not be directly interpreted as successful bacterial colonization in the intestine.

Bulge formation of the liquid film at the trailing edge of the inclined surface and its resulting particle accumulation: A numerical study

Te-Yao Chiu,Yi-Ju Chou 한국전산유체공학회 2022 한국전산유체공학회 학술대회논문집 Vol.2022 No.10

Evaluation of anisotropic deformation behaviors in H-charged Zircaloy-4 tube

Ding, Yao,Kim, Ju-Seong,Kim, Hoa,Won, Chanhee,Choi, Seungho,Park, Sung Hyuk,Yoon, Jonghun Elsevier 2018 JOURNAL OF NUCLEAR MATERIALS Vol.508 No.-

<P><B>Abstract</B></P> <P>In this paper, we focus mainly on evaluating the anisotropy evolution in Zircaloy-4 tubes with respect to hydrogen pickup which tends to affect the deformation behavior and fracture elongation due to embrittlement phenomenon induced by Zr hydrides. To capture the complex material behaviors of the H-charged (166 ppm) Zircaloy-4 tubes, we have applied the Hill anisotropic yield criterion by carrying out several material tests, such as the axial tensile, ring tensile, and axial crushing tests, for calibrating the anisotropic coefficients based on the directional strength and strain ratios. It makes possible to simulate the directional flow curves of the as-received and the H-charged Zircaloy-4 tubes based on the directional strength ratios along the axial and hoop directions, respectively, as well as a variation in deformation modes in the axial crushing test with substantial accuracy.</P>

Cooperative Learning in Small EFL Early Reading Groups

( Yu Ju Lan ),( Kuo En Chang ),( Yao Ting Sung ) 범태평양응용언어학회 2005 범태평양응용언어학회지 Vol.9 No.2

The purpose of this study is to explore the interactive behaviors and intra-group discourse in a cooperative reading group when students were asked to do meaning-based reading tasks with their group mates cooperatively. The subjects were 52 third graders from an elementary school of Taipei, Taiwan. For ten weeks, both video and audio data were collected. Based on the finding of qualitative data analysis, we found four main types of group interactive behavior which we called Ardent, Be-forced, Supervisory, and Wait-on. According to the intra-group discourse, we also found that peer-assisted learning existed in cooperative reading groups and most of the peer discourse provided essential scaffolding, modeling, and direct instruction dialogs that help children with lower reading abilities do meaning-based reading.

Comedications and potential drug-drug interactions with direct-acting antivirals in hepatitis C patients on hemodialysis

( Po-yao Hsu ),( Yu-ju Wei ),( Jia-jung Lee ),( Sheng-wen Niu ),( Jiun-chi Huang ),( Cheng-ting Hsu ),( Tyng-yuan Jang ),( Ming-lun Yeh ),( Ching-i Huang ),( Po-cheng Liang ),( Yi-hung Lin ),( Ming-ye 대한간학회 2021 Clinical and Molecular Hepatology(대한간학회지) Vol.27 No.1

Background/Aims: Direct-acting antivirals (DAAs) have been approved for hepatitis C virus (HCV) treatment in patients with end-stage renal disease (ESRD) on hemodialysis. Nevertheless, the complicated comedications and their potential drug-drug interactions (DDIs) with DAAs might limit clinical practice in this special population. Methods: The number, class, and characteristics of comedications and their potential DDIs with five DAA regimens were analyzed among HCV-viremic patients from 23 hemodialysis centers in Taiwan. Results: Of 2,015 hemodialysis patients screened in 2019, 169 patients seropositive for HCV RNA were enrolled (mean age, 65.6 years; median duration of hemodialysis, 5.8 years). All patients received at least one comedication (median number, 6; mean class number, 3.4). The most common comedication classes were ESRD-associated medications (94.1%), cardiovascular drugs (69.8%) and antidiabetic drugs (43.2%). ESRD-associated medications were excluded from DDI analysis. Sofosbuvir/velpatasvir/voxilaprevir had the highest frequency of potential contraindicated DDIs (red, 5.6%), followed by glecaprevir/pibrentasvir (4.0%), sofosbuvir/ledipasvir (1.3%), sofosbuvir/velpatasvir (1.3%), and elbasvir/grazoprevir (0.3%). For potentially significant DDIs (orange, requiring close monitoring or dose adjustments), sofosbuvir/velpatasvir/voxilaprevir had the highest frequency (19.9%), followed by sofosbuvir/ledipasvir (18.2%), glecaprevir/pibrentasvir (12.6%), sofosbuvir/velpatasvir (12.6%), and elbasvir/grazoprevir (7.3%). Overall, lipid-lowering agents were the most common comedication class with red-category DDIs to all DAA regimens (n=62), followed by cardiovascular agents (n=15), and central nervous system agents (n=10). Conclusions: HCV-viremic patients on hemodialysis had a very high prevalence of comedications with a broad spectrum, which had varied DDIs with currently available DAA regimens. Elbasvir/grazoprevir had the fewest potential DDIs, and sofosbuvir/velpatasvir/voxilaprevir had the most potential DDIs. (Clin Mol Hepatol 2021;27:186-196)

Nonlinear dynamics analysis of multi-cutting wire electrode in WEDM-HS subjected to working fluid considering the effect of debris

Sheng-Yao Fan,Qiu-Ju Zhang,Hai-Wei Chen,Wen-xuan Zeng 대한기계학회 2013 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.27 No.12

A reasonable dielectric fluid velocity and better exclusion of debris are very important to obtain a stable machining performance in multi-cutting of WEDM-HS. We investigated the dynamic characteristics of multi-cutting wire electrode in WEDM-HS subjected to working fluid considering the effect of debris. First, a fluid-solid coupling nonlinear vibration model is derived by analyzing the forces acting on the multi-cutting wire which locate in the dielectric fluid. The additional axial force arising from the transverse vibration of the multi-cutting wire is considered as a nonlinear term in the model. Considering that the debris accumulation in discharge gap severely influences the stability of multi-cutting wire electrode, the relationship between the debris volume concentration and debris-working fluid two-phase flow drag coefficient is obtained using the Einstein two-phase flow viscosity theory. Finally, the influences of several parameters on the multi-cutting wire displacement are discussed in detail.

Involvement of MicroRNA-198 Overexpression in the Poor Prognosis of Esophageal Cancer

Qi, Bo,Yao, Wen-Jian,Zhao, Bao-Sheng,Qin, Xiu-Guang,Wang, Yi,Wang, Wen-Ju,Wang, Tian-Yun,Liu, Shang-Guo,Li, Han-Chen Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.9

Objective: This study aimed to investigate whether the miR-198 expression level is related to clinicopathological factors and prognosis of esophageal cancer. Methods: MicroRNA was extracted from esophageal cancer patients who underwent surgery for assessment using the Taqman@ MicroRNA assay. The correlation between miR-198 expression and clinicopathological features was analyzed, and the significance of miR-198 as a prognostic factor and its relationship with survival was determined. Results: MicroRNA-198 (miR-198) expression was higher in patients with poor prognosis than those with good prognosis (P<0.05). Kaplan-Meier analysis results showed that the miR-198 expression level had a significant correlation with survival time (P=0.030) and that patients with a higher expression of miR-198 had a shorter survival time. Cox multi-factor model analysis showed that patient prognosis (P=0.014), tumor length (P=0.040) and expression (P=0.012), and survival time had a significant correlation; the corresponding risks were 7.268, 1.246, and 3.524, respectively. Conclusion: miR-198 overexpression is involved in the poor prognosis of esophageal cancer and can be used as a biomarker for selection of cases requiring especial attention.

Тhe use of aluminium and magnesium alloys in automotive lightweight technologies

Zhiyuan Hao,Yao Ju,Lingxiang Chen 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.9

This paper presents comparative characteristics of the aluminum and magnesium alloys in the context of their utilization in the metallurgical and automotive industry. The studies carried out in our work help to solve the problem of reducing the weight of automotive devices to reduce fuel consumption through the use of aluminum-magnesium alloys. The originality of our article lies in a comprehensive study of the stated problem: development of a research methodology, testing of the alloys under study for uniaxial tension, quantitative analysis of the Portevin-Le Chatelier effect, development of a mathematical model for optimizing parts, computer simulation of the viscoplastic properties of a model sample. Analysis of the obtained strength parameters demonstrates that the flow characteristics of the two-phase alloy of aluminum and magnesium exceed the ultimate tensile strength by 50 %. Rate of extension was inversely proportional to the intensity of the Portevin-Le Chatelier effect.