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Qian Wu,Hui Wang,Juanjuan Wu,Dagang Wang,Yongli Wang,Lei Zhang,Zhiping Huang,Deyue Yu 한국식물학회 2015 Journal of Plant Biology Vol.58 No.4
The evaluation and use of endogenous soybean genes is an effective strategy to minimize the yield losses caused by insects. Allene oxide cyclase (AOC) catalyzes the most important step in the biosynthesis of jasmonate (JA), which plays a crucial role in plant defense against insects. In this study, the role of GmAOC3 in plant insect resistance was evaluated. Real-time PCR results indicate that GmAOC3 was uniquely and rapidly activated and attained peak expression in leaves after attack by the common cutworm (CCW). In insect bioassays, transgenic lines overexpressing GmAOC3 were significantly less damaged than wild-type plants, and the relative growth rate of CCW fed with leaves from transgenic lines was significantly lower than that of CCW fed with leaves from wild-type plants. Electron microscopy revealed that the density of leaf trichomes in transgenic lines overexpressing GmAOC3 was greater than that in wild-type tobacco. Several physiological and morphological indicators, including JA, phenolic content and the relative expression levels of the putrescine N-methyltransferase (PMT) and proteinase inhibitor (PI) genes, phenylalanine ammonia lyase (PAL) activity and volatile substances, increased in the transgenic plants overexpressing GmAOC3. Our findings indicate that GmAOC3 plays an important role in soybean resistance to CCW and can be used as a resource for plant breeding.
Lewen Zhang,Deyu Guo,Jing Wu 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.8
The stability of the rocky slope is a systematic problem under the combined action of many complex and changeable factors. In this paper, a comprehensive evaluation model based on VIKOR and binary semantics is proposed, and the stability of the rocky slope is predicted and evaluated. Fristly, according to the damage mechanism and the failure process of the slope rock mass, eight influence factors, such as rock cohesion, internal friction angle, and seismic intensity, are selected as the evaluation indexes for the rocky slope stability forecast. The rocky slope stability evaluation index system has been established. Secondly, a stability forecast model of the rocky slope based on VIKOR and the binary semantics method is proposed. The visualization window and the software system of the prediction model are realized by the Python 3.5 software. Finally, the software is applied to the stability evaluation of the rocky slopes, such as Jinping I Hydropower Station and Longtan Hydropower Station. The result shows that: 1) The visual window and the software operating system of the rocky slope stability forecast model, as well as the evaluation index system replacement button are realized, which improve the calculation efficiency of the sample data and the practicability and applicability of the software system. 2) The calculation results of the rocky slope stability prediction model based on VIKOR and the binary semantics method proposed in this paper are more consistent with the actual engineering situation, and more accurate than the prediction results obtained by other evaluation methods. 3) The proposed software system of the rocky slope stability is also suitable for other geotechnical engineering such as tunnels and coal mines, and which has a good prospect of the engineering application.
[가솔린엔진] 밸브개폐시기가변에 따른 엔진 특성의 예측에 관한 연구
황재원(J.W. Hwang),김만호(M.H. Kim),박재근(J.K. Park),Wu deyu,Liu Shenghua,채재우(J.O. Chae) 한국자동차공학회 1999 한국자동차공학회 춘 추계 학술대회 논문집 Vol.- No.-
In this paper, a zero-dimensional two zone model is developed to investigate the effects of variable valve timing on combustion process in SI engine. The simulation results show that the predicted data has good agreement with experimental ones. The useful information of combustion process such like residual gas fraction, cylinder pressure, cylinder temperature and NO concentration can be obtained and the effects of engine variables on combustion processes and performances can be evaluated
Songhua Xiao,Jianwen Xu;Jun Zhao;Jianguang Liu,Jianwen Xu;Jun Zhao;Jianguang Liu,Qiaojuan Wu,Jingzhong Yu,Deyue Yu 한국식물학회 2017 Journal of Plant Biology Vol.60 No.1
In this study, we comparatively analyzed the 115 Hsp70 genes identified in Gossypium raimondii, Gossypium hirsutum and Gossypium arboreum genomes. Those Hsp70 genes unequally distributed among chromosomes in A and D genome of cotton (Gossypium spp.), and were classified into 29 groups according to the homology of them. Based on the localization information of the orthologs in Arabidopsis, the Hsp70 proteins were predicted to locate in cytosol, endoplasmic reticulum, mitochondrion or chloroplast. Homologous analysis indicated the evolutionary conservation of Hsp70 in cotton. In addition, those Hsp70 genes were differently expressed in Suyuan-045, Hai-7124 and TM-1, which were highly resistant, resistant, and sensitive to Verticillium dahliae respectively. The expressions of 26 Hsp70 genes were induced by Verticillium dahliae except for Hsp70-07/16/25/26, and the result suggested the potential involvement of them in responding to Verticillium wilt. Hsp70-08/30/31 was highly expressed in both Suyuan-045 and Hai-7124, and it was hypothesized that they might be involved in the resistance to the invasion of Verticillium dahliae. 144h after inoculation with Verticillium dahliae, the expression of Hsp70-13/14/15 was only up-regulated in Suyuan-045, and it was assumed that they might be involved in resistance to the extension of Verticillium dahliae. Further study on those Hsp70 genes would be valuable to reveal the role of them in Verticillium wilt resistance.