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Kang, Ji Min,Murnandari, Arti,Youn, Min Hye,Lee, Wonhee,Park, Ki Tae,Kim, Young Eun,Kim, Hak Joo,Kang, Seong-Pil,Lee, Jung-Hyun,Jeong, Soon Kwan Elsevier 2018 Chemical engineering journal Vol.335 No.-
<P><B>Abstract</B></P> <P>To avoid the main disadvantage of the carbon dioxide (CO<SUB>2</SUB>) capture process, namely the large amount of energy consumed to regenerate the amine absorbent using current thermal methods, chemical regeneration has been introduced as a novel method to regenerate the amine. Chemical regeneration deploys a swing in the pH of the amine absorbent rather than the swing in temperature of typical thermal regeneration procedures, and hence reduces the regeneration energy. Here we tested calcium chloride (CaCl<SUB>2</SUB>) and calcium hydroxide (Ca(OH)<SUB>2</SUB>) as a calcium source for CO<SUB>2</SUB> desorption and a pH swing agent for amine regeneration. After desorbing from the amine, CO<SUB>2</SUB> in our procedures reacted with Ca<SUP>2+</SUP> to form calcium carbonate (CaCO<SUB>3</SUB>). Forming precipitated CaCO<SUB>3</SUB> is a permanent way to sequester CO<SUB>2</SUB>. Since carbonates have a low energy level compared to CO<SUB>2</SUB>, we expect the developed method to be an economical and energy-efficient process.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A novel process which is chemical regeneration through a pH swing is proposed. </LI> <LI> The chemical regeneration shows higher efficiency of CO<SUB>2</SUB> desorption than the thermal regeneration at even room temperature. </LI> <LI> After the chemical regeneration, a value-added CaCO<SUB>3</SUB> is produced. </LI> <LI> Ca(OH)<SUB>2</SUB> in the presence of Cl<SUP>−</SUP> plays a key role in desorbing CO<SUB>2</SUB> from amine and converting it into CaCO<SUB>3</SUB>. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Expression of eIF(iso)4E having mutations in cap binding pocket give broad TuMV resistance
Jinhee Kim,Wonhee Kang,Hee-Bum Yang,Dosun Kim,Byoung-Cheorl Kang 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07
eIF4E family is well known for recessive resistance gene of potyvirus in many crops. And Turnip mosaic virus (TuMV) is one of the major viruses in Brassicaceae crops which belong to the genus Potyvirus. To elucidate the key amino acids in the interaction between TuMV VPg and Brassica eIF(iso)4E, amino acids of eIF(iso)4E were mutated. Seven amino acids in cap binding pocket were chose for the candidate amino acid that may play a role in the interaction of TuMV VPg. We demonstrated that a single amino acid mutation in cap binding pocket of Brassica eIF(iso)4E can abolish the interaction with TuMV VPg. eIF(iso)4E which has a mutation at each W49, W95 and K150 positions impaired in its interaction with VPg prominently according to the yeast two hybrid analysis. Complementation of an eIF4E knockout yeast strain by mutated eIF(iso)4E proteins showed that all eIF(iso)4E mutants were able to complement eIF4E of yeast. To find out if these mutations affect the susceptibility of Chinese cabbage, transformant analysis was performed. eIF(iso)4E W95L, W95L/K150E and susceptible wild type were over-expressed in susceptible Chinese cabbage. According to the TuMV screening result of T1 and T2 transformants, over-expression of the eIF(iso)4E mutants showed resistance to four TuMV strains (CHN2, 3, 4 and 5). Our results support that the mutations in eIF(iso)4E may control the broad spectrum TuMV resistance.
Lee, Wonhee,Kim, Ki-Sub,Kang, Seong-Pil,Kim, Jong-Nam American Chemical Society 2018 Energy & fuels Vol.32 No.4
<P>In our previous studies, it was proven that ionic liquids can be a good synergist with poly(<I>N</I>-vinylcaprolactam) [PVCap, a polymeric kinetic hydrate inhibitor (KHI)] against methane or natural gas hydrate formation. In particular, 1-hexyl-1-methylpyrrolidinium tetrafluoroborate (HMP-BF<SUB>4</SUB>) showed the best performance for natural gas hydrate inhibition among the ionic liquids tested. In this study, we report the result of performance tests on kinetic inhibition of natural gas hydrate formation with a mixture of PVCap and HMP-BF<SUB>4</SUB> in the presence of an additional oil phase. At various water cuts (20, 50, and 80%) with 3.5 wt % aqueous NaCl solution and mineral oil, the KHI performance of PVCap alone and a mixture of 0.5 wt % PVCap and 0.5 wt % HMP-BF<SUB>4</SUB> was evaluated. This was performed by measuring the induction time for the synthetic natural gas hydrate formation, in addition to the pressure, temperature, and torque of the system investigated. Calculations were carried out to determine the amount of water converted to hydrate and the volume fraction of hydrate in the liquid phase. The results showed that the induction time became shorter when no KHIs were tried as the water cut increased and that the trend was identical with a further diminished induction time with PVCap alone. On the other hand, the mixture of PVCap and HMP-BF<SUB>4</SUB> showed an effective synergetic performance on the kinetic inhibition of natural gas hydrate formation (increased induction time). Moreover, the KHI mixture showed a longer induction time as the water cut increased. This opposite tendency in the induction time according to the water cuts and KHI applied was explained by the hydrate nucleation rate per unit volume of the aqueous phase.</P> [FIG OMISSION]</BR>
공중보건증진, 개인의 선택권, 환경보호 가치의 충돌로 인한 정책옹호연합의 형성과 변화
임원희,김희정,강민아 이화여자대학교 생명의료법연구소 2013 생명윤리정책연구 Vol.7 No.2
In this paper, we applied the advocacy coalition framework (ACF) to analyze the policy changes surrounding the fluoridation program in Korea. While fluoridation programs were actively pursed by the Ministry of Health and Welfare in Korea to promote preventive public health efforts, opponents raised questions on the safety of the fluoride and argued that implementation of the program violates individuals’ right to refuse fluoride treatment. Opponents, especially environmentalists, also raised issues with potential harms to environment. As commonly found in the ACF analysis, a diverse array of coalitions were formed, taking positions on the fluoridation program, based on their core values surrounding the issue, such as public responsibility to protect health and prevent diseases of vulnerable groups versus protection of individual rights and environment from potential harms. To note, we were able to find coalitions’ persistent and active efforts to influence decision-making process with new knowledge and information regarding the effectiveness, safety, and efficiencies of the fluoridation program. The prominent role of knowledge transfer and validation should be evaluated further in future research to understand the impact of such strategies.