흰쥐에서 염화비닐 폭로수준에 따른 소변내 thiodiglycolic acid 농도의 변화

왕승준,차봉석,노재훈,신동천,김명수,전근재 大韓産業醫學會 1999 대한직업환경의학회지 Vol.11 No.4

Objectives : It is the objective of this research to identify the variation of thiodiglycolic acid(TdGA) in urine with vinyl chloride monamer(VCM) exposure levels through methylation. Methods : After rats were exposed to vinyl chloride monomer of 4 levels, 0㎎/㎥, 50㎎/㎥, 150㎎/㎥, 500㎎/㎥, respectively, of which urine was sampled in each sampling time of 0 hour, 2 hours, 4 hours, 8 hours, 16 hours, 36 hours, 48 hours. After urine had been methylated with diazomethane in the preliminary experiment and the peak of 146 m/z had been verified, the main experiment was done. Results : In the variation of TdGA with sampling times, concentration of TdGA increased rapidly in 4 hours and then decreased after 8 hours. When the variation of urinary TdGA concentration in urine according to exposure level of VGM was verified through Kruskal-Wallis statistical method at each sampling time, the significant increment with the exposure levels at 2 hours, 4 hours, 8 hours after exposure was clarified. Conclusion : TdGA concentration in urine with increment of VCM exposure level increased, especially significantly at 2 hours, 4 hours, 8 hours of sampling time.

乳齒列期 齒列穹 形態에 關한 硏究

金旺根,金南洪 大韓小兒齒科學會 1985 大韓小兒齒科學會誌 Vol.12 No.1

This study was to investigate the fitness of the primary dental arch to the parabola. The samples of 91 plaster casts were obtained which were taken from children with normal occlusion, aging from 3 to 5 years. Landmarks on the stone casts were marked on measuring glass which was superimposed on the stone casts, and transfered and measured to the tracing paper. The points on the tracing papers were digitized, and scattergrams and statistics were performed by the computer; PRIME 750, IBM Co., U.S.A.. The results were as follows: 1. The fitness of the primary ental arch to the parabola was above 92% in the upper dental arch, and was above 91% in the lower dental arch. 2. Constant value A of upper dental arch line passing the midpoints of primary dentition was -0.54 and that of lower ental arch was -0.59, and the upper dental arch showed more expended parabola than the lower dental arch. 3. The dental arch line passing the buccal cusp tips of the primary posteriors and the midpoints of he primary incisors were more fit to the parabola than the dental arch line passing the midpoints of the primary teeth. 4. The ratio of oblique arch length to intermilar width was divided into 4 groups by the quaters, and the mean parabolic equations and curves were calculated and drawn.

신뢰성 제약조건을 가진 평면 트러스의 최적 설계

변근주,김왕구,한상훈 연세대학교 산업기술연구소 1981 논문집 Vol.13 No.1

The aim in this presentation is to explore the relationship between optimum design of structures and reliability of structures. In this paper, the concept of probability of failure is explained for a general structural system. The concept emphasized in the paper is the employment of probability of failure constraint in the mathematical programming problem to determine the set of design parameters which minimizes the weight of the truss structures. Specific reliability based optima for multi-member systems are shown for weakest-link structures which fail if any element fails. Several examples of optimization with failure probability constraints are presented, and then the sensitivity of optimum design to reliability level as well as parameters in the reliability analysis is discussed. The results presented indicate: 1) The results indicate the feasibility of using probability of failure constraints in solving for optimum m lti-member structural designs. 2) An important factor influencing the magnitude of the optimum design as well as its member sizes will be the choice of load and strength frequency distributions and their parameters particulary the coefficients of variation. 3) The optimum value increases with both allowable failure probability and increase in coefficients of variation. 4) The classical approach to optimum design using a safety factor to represent conservation often results in unnecessary weight and cost.

방사선상 enhancement 정도에 따른 측모두부방사선규격사진 계측점 설정의 재현도

유황석,황현식,김왕식,김경근 전남대학교 치의학연구소 2001 구강과학 Vol.13 No.4

The purpose of this study was to evaluate the reproducibility of lateral cephalometric landmarks according to radiographic image enhancement, and to contribute to the identification of cephalometric landmarks. Lateral cephalograms of ten individuals were taken and stored into computer. The images were then enhanced up to four grades by Quick Ceph Image Pro^TM on condition that the gray-scale equalization number was 50 and the detail enhancement number was 50. After thirty two landmarks were identified on monitor images by five observers, the deviations from the mean, the distances estimated between identified points and the mean point of five identified points, were evaluated for each landmark at each enhancement grade. Through the statistical analysis, following results were obtained. 1. In case of unenhanced radiographic images, the inter-observer reproducibility of the landmarks showed a large variation. 2. The comparison of deviation from the mean according to the degree of radiographic image enhancement for each landmark showed that the inter-observer reproducibility was significantly different at 5 landmarks. 3. The landmark of pterygomaxillary fissure showed higher reproducibility at enhancement grade 1 and 2 images that at unenhanced images. So did the landmark of posterior nasal spine at enhancement grade 1 images, and the landmark of menton at enhancement grade 2, 3 and 4 images respectively. The above results suggest that the reproducibility of some landmarks can be increased by radiographic image enhancement during the identification of the lateral cephalometric landmarks on the monitor.

췌담관 합류이상이 있는 환자에서 담도계 손상 기전에 대한 연구

정인호,정용식,김지훈,최수윤,김정운,김욱환,홍정,왕희정,유병무,김진홍,한재호,김영배,김재근,김명욱 한국간담췌외과학회 2003 한국간담췌외과학회지 Vol.7 No.2

Physiological and Proteomics Analysis to Potassium Starvation in Rice

Kim, Sang-Gon,Wang, Yiming,Lee, Chang-Hoon,Chi, Yong-Hun,Kim, Keun-Ki,Choi, In-Soo,Kim, Yong-Chul,Kang, Kyu-Young,Kim, Sun-Tae The Korean Society of Environmental Agriculture 2011 한국환경농학회지 Vol.30 No.4

BACKGROUND: Potassium (K) is one of the macronutrients which are essential for plant growth and development. Its deficiency in paddy soils is becoming one of the limiting factors for increasing rice yield in Asia. METHODS AND RESULTS: To investigate physiological symptoms under K-starvation (NP) compared with complete media (NPK) condition, we measured shoot/root length, weight, nutrients, and patterns of protein expression. The shoot growth was significantly reduced, but root growth was not affected by K-starvation. However, biomasses were decreased in both shoot and root. Uptake of K was reduced up to 85%, while total concentrations of P, Ca, Mg, Na were increased in root and shoot. To better understand the starved K mechanism of rice, comparative proteome analysis for proteins isolated from rice leaves was conducted using 2-DGE. Five spots of differentially expressed proteins were analyzed by MALDI-TOF MS. Analysis of these K-starvation response proteins suggested that they were involved in metabolism and defense. CONCLUSION(s): Physiological and 2-DGE based proteomics approach used in our study results in observation of morphology or nutrients change and identification of K-starvation responsive proteins in rice root. These results have important roles in maintaining nutrient homeostasis and would also be useful for further characterization of protein function in plant K nutrition.

Mitochondria-Targeted Vitamin E Protects Skin from UVB-Irradiation

Kim, Won-Serk,Kim, Ikyon,Kim, Wang-Kyun,Choi, Ju-Yeon,Kim, Doo Yeong,Moon, Sung-Guk,Min, Hyung-Keun,Song, Min-Kyu,Sung, Jong-Hyuk The Korean Society of Applied Pharmacology 2016 Biomolecules & Therapeutics(구 응용약물학회지) Vol.24 No.3

Mitochondria-targeted vitamin E (MVE) is designed to accumulate within mitochondria and is applied to decrease mitochondrial oxidative damage. However, the protective effects of MVE in skin cells have not been identified. We investigated the protective effect of MVE against UVB in dermal fibroblasts and immortalized human keratinocyte cell line (HaCaT). In addition, we studied the wound-healing effect of MVE in animal models. We found that MVE increased the proliferation and survival of fibroblasts at low concentration (i.e., nM ranges). In addition, MVE increased collagen production and downregulated matrix metalloproteinase1. MVE also increased the proliferation and survival of HaCaT cells. UVB increased reactive oxygen species (ROS) production in fibroblasts and HaCaT cells, while MVE decreased ROS production at low concentration. In an animal experiment, MVE accelerated wound healing from laser-induced skin damage. These results collectively suggest that low dose MVE protects skin from UVB irradiation. Therefore, MVE can be developed as a cosmetic raw material.

Effects of Urea, Ammonium Nitrate and Calcium Silicate on the Absorption, Translocation of Potassium and Yield of Chinese Cabbage(Brassica Perkinnensis, Var. Samjin)

Wang-Keun Oh(吳旺根),Jae-Young Kim(金載英),Sung-Bae Kim(金聲培) 한국토양비료학회 1987 한국토양비료학회지 Vol.20 No.4

Gender-dependent hepatic alterations in H-<i>ras</i>12V transgenic mice

Wang, Ai-Guo,Moon, Hyung-Bae,Lee, Mi-Ran,Hwang, Chae Young,Kwon, Ki-Sun,Yu, Seong-Lan,Kim, Yong-Sung,Kim, Mirang,Kim, Jin-Man,Kim, Sang-Keun,Lee, Tae-Hoon,Moon, Eun-Yi,Lee, Dong-Seok,Yu, Dae-Yeul Elsevier 2005 Journal of hepatology Vol.43 No.5

<P><B>Background/Aims</B></P><P>Although it has been proposed that Ras and related signal pathways play important roles in hepatocarcinogenesis, appropriate in vivo models are lacking.</P><P><B>Methods</B></P><P>Two hepatocellular carcinoma lines were established using pronuclear microinjection techniques to create an insertion of the H-<I>ras</I>12V transgene under the control of the albumin enhancer/promoter. The resulting phenotypes and related molecular events were then examined.</P><P><B>Results</B></P><P>Male (but not female) transgenic mice older than 2 months showed hepatic alterations with a high degree of reproducibility, as compared to the wild-type mice. The liver/body-weight ratios were lower for the females than for the males. The transgene-carrying line 28 was investigated extensively with respect to molecular differences between the genders. Male hepatocytes showed higher Ras activity and higher reactive oxygen species (ROS) levels than female hepatocytes. The female hepatocytes showed higher expression levels of p53 and p21<SUP>Waf1/Cip1</SUP>, enhanced cytochrome <I>c</I> release, which correlated with cell cycle arrest, and higher levels of hypodiploid cell formation, as compared to the male hepatocytes.</P><P><B>Conclusions</B></P><P>The gender-related differences in molecular responses to activated Ras may have implications for the prevalence of hepatic alterations in males. Our transgenic mice represent a potentially valuable animal model for future investigations.</P>

Effect of Phytohormones and Chemical Inhibitors on Pathogenesis-related Genes Identified by Differential Hybridization in Rice Suspension Culture Cells

Kim, Sang-Gon,Wu, Jing-Ni,Wang, Yiming,White, Ethan E.,Choi, Young-Whan,Kim, Keun-Ki,Choi, In-Soo,Kim, Yong-Cheol,Kim, Sun-Hyung,Kang, Kyu-Young,Kim, Sun-Tae The Korean Society of Plant Pathology 2010 Plant Pathology Journal Vol.26 No.4

In order to study disease resistance mechanisms in rice against the rice blast fungus Magnaporthe grisea, we screened fungal elicitor-responsive genes from rice suspension-cultured cells treated with fungal elicitors employing differential hybridization (DH). By DH screening, 31 distinct rice clones were isolated and a majority of them were full-length cDNAs encoding pathogenesisrelated (PR) genes. Sixteen of the 31 genes were upregulated at 4, 8, and 12 h following fungal elicitor treatment. To elucidate the effect of signal molecules and biotic elicitors on the regulation of rice defense genes, we further characterized the transcriptional expression patterns of representative isolated PR genes; OsGlu1, OsGlu2, OsTLP, OsRLK, and OsPR-10, following treatment with fungal elicitor, phytohormones, cycloheximide, and inhibitors of protein phosphorylation. Jasmonic acid (JA) induced transcriptional expression of OsGlu1, OsTLP, and OsRLK, but not of OsGlu2 and OsPR-10 at any of the tested time points. Salicylic acid (SA) and abscisic acid weakly induced the expression of OsTLP and OsRLK. SA showed an antagonistic effect with fungal elicitor and JA. Cycloheximide suppressed all these genes upon elicitor treatment, except for OsGlu2. Staurosporine only induced the expression of OsRLK. Application of calyculin A strongly induced OsRLK expression, but suppressed the expression of OsGlu2. Our study yielded a number of PR genes that play a role in defense mechanisms against the rice blast fungus, as well as contribute towards the elucidation of crosstalk between phytohormones and other modifications during defense signaling.