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Natiele Carla da Silva Ferreira,Roˆmulo Jose´ Soares-Bezerra,Rebeca Ferreira Couto da Silveira,Clayton Menezes da Silva,Carla Santos de Oliveira,Andrea Surrage Calheiros,Taˆnia Maria Alves,Carlos Leom 한국식품영양과학회 2019 Journal of medicinal food Vol.22 No.2
P2Y2 and P2Y4 receptors are physiologically activated by uridine 5′-triphosphate (UTP) and are widely expressed in many cell types in humans. P2Y2 plays an important role in inflammation and proliferation of tumor cells, which could be attenuated with the use of antagonists. However, little is known about the physiological functions related to P2Y4, due to the lack of selective ligands for these receptors. This can be solved through the search for novel compounds with antagonistic activity. The aim of this study was to discover new potential antagonist candidates for P2Y2 and P2Y4 receptors from natural products. We applied a calcium measurement methodology to identify new antagonist candidates for these receptors. First, we established optimal conditions for the calcium assay using J774.G8, a murine macrophage cell line, which expresses functional P2Y2 and P2Y4 receptors and then, we performed the screening of plant extracts at a cutoff concentration of 50 μg/mL. ATP and ionomycin, known intracellular calcium inductors, were used to stimulate cells. The calculated EC50 were 11 μM and 103 nM, respectively. These cells also responded to the UTP stimulation with an EC50 of 1.021 μM. Screening assays were performed and a total of 100 extracts from Brazilian plants were tested. Joannesia princeps Vell. (stem) and Peixotoa A. Juss (flower and leaf) extracts stood out due to their ability to inhibit UTP-induced responses without causing cytotoxicity, and presented an IC50 of 32.32, 14.99, and 12.98 μg/mL, respectively. Collectively, our results point to the discovery of potential antagonist candidates from Brazilian flora for UTP-activated receptors.
Leite Luciano Bernardes,Soares Leôncio Lopes,Portes Alexandre Martins Oliveira,Soares Thayana Inácia,da Silva Bruna Aparecida Fonseca,Dias Taís Rodrigues,Costa Sebastião Felipe Ferreira,Guimarães-Ervi 대한고혈압학회 2024 Clinical Hypertension Vol.30 No.-
Background Under the adverse remodeling of the right ventricle and interventricular septum in pulmonary arterial hypertension (PAH) the left ventricle (LV) dynamics is impaired. Despite the benefts of combined aerobic and resist‑ ance physical trainings to individuals with PAH, its impact on the LV is not fully understood. Objective To test whether moderate-intensity combined physical training performed during the development of PAH induced by MCT in rats is benefcial to the LV’s structure and function. Methods Male Wistar rats were divided into two groups: Sedentary Hypertensive Survival (SHS, n=7); and Exercise Hypertensive Survival (EHS, n=7) to test survival. To investigate the efects of combined physical training, another group of rats were divided into three groups: Sedentary Control (SC, n=7); Sedentary Hypertensive (SH, n=7); and Exercise Hypertensive (EH, n=7). PAH was induced through an intraperitoneal injection of MCT (60 mg/kg). Echocardiographic evaluations were conducted on the 22nd day after MCT administration. Animals in the EHS and EH groups participated in a combined physical training program, alternating aerobic (treadmill running: 50 min, 60% maximum running speed) and resistance (ladder climbing: 15 climbs with 1 min interval, 60% maximum carrying load) exercises, one session/day, 5 days/week for approximately 4 weeks. Results The physical training increased survival and tolerance to aerobic (i.e., maximum running speed) and resist‑ ance (i.e., maximum carrying load) exertions and prevented reductions in ejection fraction and fractional shortening. In addition, the physical training mitigated oxidative stress (i.e., CAT, SOD and MDA) and inhibited adverse LV remod‑ eling (i.e., Collagen, extracellular matrix, and cell dimensions). Moreover, the physical training preserved the ampli‑ tude and velocity of contraction and hindered the reductions in the amplitude and velocity of the intracellular Ca2+ transient in LV single myocytes. Conclusion Moderate-intensity combined physical training performed during the development of MCT-induced PAH in rats protects their LV from damages to its structure and function and hence increases their tolerance to physi‑ cal exertion and prolongs their survival.
( Li-da Chen ),( Ta-te Lin ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1
Milk fat is one of the most important animal oils in the food industry due to its high nutritional value and market cost. However, when affected by adulteration, it leads to economic loss for food manufacturers, and moreover, causing harm to human health. This research demonstrates the application of hyperspectral and fluorescence imaging in assessing the concentration of animal and plant oil mixture. Milk fat samples were adulterated with different plant oil samples at different concentrations. Spectrum analysis was performed using hyperspectral imaging and fluorescence imaging with light source excitation obtained from an Excitation Emission Matrix (EEM). A model was developed using Artificial Neural Networks (ANN) to determine what kind of plant oil is being adulterated, while Support Vector Regression (SVR) was used to determine the actual concentration of the oil mixture. This technique can serve as a rapid detection tool to help speed up the traditional inspection process.
CONSUMER DISPOSITIONS IN MULTICULTURAL CONTEXTS: A FRAMEWORK PROPOSITION
Mariana Bussab Porto-da-Rocha,Sergio Garrido Moraes,Miriam Ta?s Salom?o,Vivian Iara Strehlau 글로벌지식마케팅경영학회 2018 Global Marketing Conference Vol.2018 No.07
Globalization has coined cultural flows across national borders, resulting in different behaviors towards foreign products. Different approaches explain consumption in multicultural contexts such as consumer disposition. Based on literature review, this study organizes different dispositions constructs in relation to one’s own country and to foreign countries proposing a conceptual framework.
Antinociceptive profile of the ethanol extract of andrographis paniculata in mice
MR Sulaiman,,S Sainan,,ZA Zakaria,,MN Somchit,,DA Israf,,S Moin,TA Tengku Mohamad 경희대학교 융합한의과학연구소 2007 Oriental Pharmacy and Experimental Medicine Vol.7 No.4
The present study was conducted to evaluate the analgesic activity of ethanol extract of Andrographis paniculata (AP) in mice. The analgesic investigations were carried out using the acetic acid-induced abdominal writhing and the hot-plate tests. It was demonstrated that intraperitoneal (i.p.) administration of the extract at a dose of 30, 100, 300, 500 mg/kg, produced significant inhibition of abdominal constriction induced with 0.6% (v/v) acetic acid in dosedependent manner. It also demonstrated that the extract produced significant dose-dependent increase in the time of latency to a discomfort reaction in the hot-plate model. In addition, the analgesic effect of the ethanolic extract of AP was significantly reversed by a non-specific opioid receptor antagonist, naloxone. These results indicate that AP has an analgesic effect that was mediated through opioid receptors.
Antinociceptive profile of the ethanolic extract of andrographis paniculata in mice
Sulaiman, MR,Sainan, S,Zakaria, ZA,Somchit, MN,Israf, DA,Moin, S,Mohamad, TA Tengky Kyung Hee Oriental Medicine Research Center 2007 Oriental pharmacy and experimental medicine Vol.7 No.4
The present study was conducted to evaluate the analgesic activity of ethanolic extract of Andrographis paniculata (AP) in mice. The analgesic investigations were carried out using the acetic acid-induced abdominal writhing and the hot-plate tests. It was demonstrated that intraperitoneal (i.p.) administration of the extract at a dose of 30, 100, 300, 500 mg/kg, produced significant inhibition of abdominal constriction induced with 0.6% (v/v) acetic acid in dosedependent manner. It also demonstrated that the extract produced significant dose-dependent increase in the time of latency to a discomfort reaction in the hot-plate model. In addition, the analgesic effect of the ethanolic extract of AP was significantly reversed by a non-specific opioid receptor antagonist, naloxone. These results indicate that AP has an analgesic effect that was mediated through opioid receptors.