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Keewoo Lee,Sungwhan Cho,이혁수,Jae Young Choi,Yeon Ho Je,Yonggyun Kim 한국응용곤충학회 2005 Journal of Asia-Pacific Entomology Vol.8 No.3
A genome project has been launched andaims to sequence total genome of Cotesia plutellae bracovirus (CpBV). This on-going research has iden-tified several open reading frames (ORFs) including an analyze gene expression of CpBV-ELP1 in the para-sitized diamondback moth, Plutella xylostela. CpBV- ELP genomic DNA contains one intron (78 bp long). Its ORF consists of 726 bp encoding 241 amino acidresidues. The hypothetical CpBV-ELP1 protein ispredicted as 27,787.83 Da and poseses N-terminal signal peptide plus three potential N-glycosylation sites. Its amino acid sequence exhibits high homology with EP1 genes from C. congregata or C. karyai bA reverse transcriptase-polymerase chain reaction(RT-PCR) indicated that CpBV-ELP1 was expressedonly in P. xylostella parasitized by C. plutellae. The expression levels were measured by real time quan-titative RT-PCRs during entire parasitization period at 25℃ culturing temperature. The expression began at the first day of parasitization and increased with the parasitization period. The ORF PCR product wascloned, over-expressed, and molecular weight of thepurified protein was about 30 kDa.
이형돈(Hyungdon Lee),윤혁진(Hyunkjin Yoon),조성환(Sungwhan Cho) 한국신재생에너지학회 2021 한국신재생에너지학회 학술대회논문집 Vol.2021 No.7
식물성 및 동물성 기름과 같은 바이오연료는 지속적 생산이 가능한 장점이 있으나, 산업용으로 활용하기 위해서는 기름특성(높은 점도, 인화점 등)으로 인해, 기존 연소방식으로 상용화하는 것이 매우 힘들다. 본 연구에서는 직업 연소가 어려운 폐 바이오연료를 사용해 가정용 또는 산업용으로 사용가능한 난방장치를 개발하고자 하였다. 특히, 핵심 연소기에 대한 3차원 모델링 검토와 열효율을 높이기 위한 구조 설계기법을 활용해, 제품의 활용도를 높이고 연소최적화 설계를 진행하였다. 또한 일반 난방장치와 튜브형 히터장치 2종의 모듈화 설계를 통해 2만kcal이상의 열량을 확보하고자 최적화 연구를 진행중이며, 자동점화장치를 통해 점화안정화 및 내구성을 증가시킬 수 있는 구조화 설계를 수행하고 있다.
Choi, Ji-Hyuk,Lee, Chongmin,Cho, Sungwhan,Moon, Geon Dae,kim, Byung-su,Chang, Hankwon,Jang, Hee Dong Elsevier 2018 Carbon Vol.132 No.-
<P><B>Abstract</B></P> <P>Reduced graphene oxide (RGO) is employed as a conductive binder for the unprecedentedly high performance of the used coffee grounds-derived AC (cAC) supercapacitor. The 2 wt% of RGO sheets in the cAC based electrode (cAC-2) successfully preserves high specific surface area of cAC and supports conductive networks decorated with oxygen groups, which synergistically improve charge storage capability. Furthermore, the wettability of the cAC-2 electrode enhanced by hydrophilic oxygen groups of RGO allows the easy access of the electrolyte to the pores of the electrode. The symmetric two electrode system exhibits outstanding charge storage capacity, reaching a high specific capacitance of 512 F g<SUP>−1</SUP> in KOH electrolyte at 0.5 A g<SUP>−1</SUP> which is nearly four times higher than that of cAC electrode with PVDF binders. Remarkable electrochemical performance is further demonstrated in EMIMTFSI electrolyte, including high specific capacitance of 440 F g<SUP>−1</SUP> at 0.5 A g<SUP>-1</SUP>, with an excellent rate performance of 81.4% from 0.5 to 10 A g<SUP>-1</SUP>. The record high energy density of 187.3 Wh kg<SUP>−1</SUP> is achieved at a power density of 438 W kg<SUP>−1</SUP> and maintains an energy density of 153.8 Wh kg<SUP>−1</SUP> at 8750 W kg<SUP>−1</SUP> in EMIMTFSI electrolyte.</P> <P><B>Graphical abstract</B></P> <P>Novel-green electrode composed of biomass-derived activated carbons and RGO sheets as a binder was fabricated for ultrahigh performance supercapacitors.</P> <P>[DISPLAY OMISSION]</P>
Analysis of Syndecan-2 Methylation in Bowel Lavage Fluid for the Detection of Colorectal Neoplasm
( Young Sook Park ),( Dong Shin Kim ),( Sang Woo Cho ),( Jong Won Park ),( Sang Jin Jeon ),( Tae Ju Moon ),( Seong Hwan Kim ),( Byoung Kwan Son ),( Tae Jeong Oh ),( Sungwhan An ),( Jeong Hwan Kim ),( 대한간학회 2018 Gut and Liver Vol.12 No.5
Background/Aims: Syndecan-2 (SDC2 ) methylation was previously reported as a sensitive serologic biomarker for the early detection of colorectal cancer (CRC). The purpose of this study was to investigate whether SDC2 methylation is detectable in precancerous lesions and to determine the feasibility of using SDC2 methylation for the detection of CRC and precancerous lesions in bowel lavage fluid (BLF). Methods: A total of 190 BLF samples were collected from the rectum at the beginning of colonoscopy from patients with colorectal neoplasm and healthy normal individuals. Fourteen polypectomy specimens were obtained during colonoscopy. A bisulfite pyrosequencing assay and quantitative methylation-specific polymerase chain reaction were conducted to measure SDC2 methylation in tissues and BLF DNA. Results: SDC2 methylation was positive in 100% of villous adenoma (VA) and high-grade dysplasia, and hyperplastic polyp samples; 88.9% of tubular adenoma samples; and 0% of normal mucosa samples. In the BLF DNA test for SDC2 methylation, the sensitivity for detecting CRC and VA was 80.0% and 64.7%, respectively, at a specificity of 88.9%. The BLF of patients with multiple tubular adenomas, single tubular adenoma and hyperplastic polyps showed 62.8%, 26.7% and 28.6% rates of methylation-positive SDC2, respectively. Conclusions: Our results demonstrated that SDC2 methylation was a frequent event in precancerous lesions and showed high potential in BLF for detecting patients with colorectal neoplasm. (Gut Liver 2018;12:508-515)