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Keewoo Lee,Sungwhan Cho,이혁수,Jae Young Choi,Yeon Ho Je,Yonggyun Kim 한국응용곤충학회 2005 Journal of Asia-Pacific Entomology Vol.8 No.3
A genome project has been launched andaims to sequence total genome of Cotesia plutellae bracovirus (CpBV). This on-going research has iden-tified several open reading frames (ORFs) including an analyze gene expression of CpBV-ELP1 in the para-sitized diamondback moth, Plutella xylostela. CpBV- ELP genomic DNA contains one intron (78 bp long). Its ORF consists of 726 bp encoding 241 amino acidresidues. The hypothetical CpBV-ELP1 protein ispredicted as 27,787.83 Da and poseses N-terminal signal peptide plus three potential N-glycosylation sites. Its amino acid sequence exhibits high homology with EP1 genes from C. congregata or C. karyai bA reverse transcriptase-polymerase chain reaction(RT-PCR) indicated that CpBV-ELP1 was expressedonly in P. xylostella parasitized by C. plutellae. The expression levels were measured by real time quan-titative RT-PCRs during entire parasitization period at 25℃ culturing temperature. The expression began at the first day of parasitization and increased with the parasitization period. The ORF PCR product wascloned, over-expressed, and molecular weight of thepurified protein was about 30 kDa.
Storage Proteins of Diamondback Moth, Plutella xylostella:Purification and Expression Profile
Ahmed M. A. Ibrahim,Keewoo Lee,이혁수,Yonggyun Kim 한국응용곤충학회 2006 Journal of Asia-Pacific Entomology Vol.9 No.2
Storage proteins (SPs) were significantly detected in the hemolymph during the late instar larvae of diamondback moth, Plutella xylostella. These SPs were resolved into three proteins (SP1, SP2, and SP3) at 7% SDS-PAGE. Their apparent molecular sizes were around 80 kDa. SP1 was synthesized later than SP2 and SP3 during the development of the last instar. Total soluble proteins of last instar larvae were extracted and fractionated sequentially with ammonium sulfate, size-exclusion chromatography, and ion-exchange chromatography. The SPs were purified and their developmental expression was discussed.
Cheon, Jung Hee,Kim, Dongwoo,Kim, Duhyeong,Lee, Keewoo Korean Mathematical Society 2022 대한수학회지 Vol.59 No.3
The scaled inverse of a nonzero element a(x) ∈ ℤ[x]/f(x), where f(x) is an irreducible polynomial over ℤ, is the element b(x) ∈ ℤ[x]/f(x) such that a(x)b(x) = c (mod f(x)) for the smallest possible positive integer scale c. In this paper, we investigate the scaled inverse of (x<sup>i</sup> - x<sup>j</sup>) modulo cyclotomic polynomial of the form Φ<sub>p<sup>s</sup></sub> (x) or Φ<sub>p<sup>s</sup>q<sup>t</sup></sub> (x), where p, q are primes with p < q and s, t are positive integers. Our main results are that the coefficient size of the scaled inverse of (x<sup>i</sup> - x<sup>j</sup>) is bounded by p - 1 with the scale p modulo Φ<sub>p<sup>s</sup></sub> (x), and is bounded by q - 1 with the scale not greater than q modulo Φ<sub>p<sup>s</sup>q<sup>t</sup></sub> (x). Previously, the analogous result on cyclotomic polynomials of the form Φ<sub>2<sup>n</sup></sub> (x) gave rise to many lattice-based cryptosystems, especially, zero-knowledge proofs. Our result provides more flexible choice of cyclotomic polynomials in such cryptosystems. Along the way of proving the theorems, we also prove several properties of {x<sup>k</sup>}<sub>k∈ℤ</sub> in ℤ[x]/Φ<sub>pq</sub>(x) which might be of independent interest.