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Jang-Eun Cho,Sang-Nae Cho,Sungae Cho 대한의생명과학회 2014 Biomedical Science Letters Vol.20 No.4
Mycobacterium tuberculosis (MTB) resides and replicates inside macrophages. In our previous report, we reported that CD8+ T cell-mediated immune responses specific for the peptide derived from MTB RNA polymerase beta-subunit (RpoB127-135) could be induced in TB patients expressing HLA-A*0201 subtype. In order to examine whether RpoB127-135 specific CD8+ T cells can recognize MTB infected macrophages in vitro, CD8+ T cell lines specific for RpoB127-135 peptide were generated from peripheral blood mononuclear cells (PBMCs) of healthy HLA-A*0201 subjects by in vitro immunization technique. In this study, we observed RpoB127-135 specific CD8+ T cells could recognize and destroy macrophages infected with MTB for 2 to 4 days. RpoB127-135 specific CD8+ T cell immune response was inducible from PBMC of healthy subjects expressing HLA-A*0206 subtype, one of HLA-A2 supertype members. Next, we investigated the HLA-I processing mechanism of RpoB127-135 peptide in MTB infected macrophages. As a result, the presentation of the MTB derived epitope peptide, RpoB127-135, to CD8+ T cells was not inhibited by the treatment with brefeldin-A (ER-Golgi transport inhibitor) or lactacystin (proteasome inhibitor), which blocks the classical HLA-I processing pathway. However, RpoB127-135 specific CD8+ T cell activity was blocked either by the blocking agent for the endocytosis (cytochalasin D) or by the blocking antibody (W6/32) for HLA-I molecules. Therefore, the RpoB127-135 peptide may be processed by accessing the alternate HLA-I processing pathway. Understanding the processing and presentation mechanisms of the MTB derived proteins will help to improve the efficacy of vaccines and the efficiency of therapeutic agents for TB.
Jang-Eun Cho,Young Sam Kim,Moo Suk Park,Kyung Wha Lee,Eunhee Lee,Sang-Nae Cho,Sungae Cho 대한의생명과학회 2007 Biomedical Science Letters Vol.13 No.4
Cell-mediated immune response (CMI) is a major immune protective mechanism against tuberculosis (TB) infection. Among several components involved in CMI, recent studies suggest that CD8+ T cells are important in controlling TB infection. In our previous report, we defined four Mycobacterium tuberculosis (MTB) derived epiotpe peptides specific for HLA-A*0201-restricted CD8+ T cells. These four peptides are PstA175-83, ThyA30-38, RpoB127-135 and 85B15-23. In this study, these epitope peptides specific CD8+ T cell responses in tuberculous pleurisy were investigated using ex vivo IFN-γ elispot assay and intracellular IFN-γ staining method. As a result, we observed these epitope peptide specific CD8+ T cell responses are induced in all three patients with tuberculous pleurisy suggesting that CD8+ T cells are involved in protective immune mechanism against MTB infection in tuberculous pleurisy. However, the CMI to mitogens and MTB antigens from pleural fluids of patients with tuberculous pleurisy does not seem to correlate with that from peripheral blood, although the sample size is too small to make any conclusion. In sum, the MHC I restricted CD8+ T cell responses seem to be induced efficiently in the pleural fluids, at the site of TB infection, in which the CMI is actively induced. In addition, these experiments suggest that MHC I restricted CD8+ T cell mediated immune responses are also involved in protective mechanism against MTB infection in extra-pulmonary TB.
조장은,이경화,박승규,천선희,조상래,조성애,Cho, Jang-Eun,Lee, Kyung Wha,Park, Seung Kyu,Cheon, Seon-Hee,Cho, Sang-Nae,Cho, Sungae 대한면역학회 2003 Immune Network Vol.3 No.3
Background: The protective immunity against tuberculosis (TB) involves both CD4+ T cells and CD8+ T cells. In our previous study, we defined four Mycobacterium tuberculosis derived peptide epitopes specific for HLA-$A^*0201$ restricted CD8+ T cells ($ThyA_{30-38}$, $RpoB_{127-135}$, $85B_{15-23}$, $PstA1_{75-83}$). In this study, we investigated the immune responses induced by these peptide specific CD8+ T cells in latently and chronically infected people with TB. Methods: We characterized these peptide specific CD8+ T cell population present in PBMC of both TB patients and PPD+healthy people using IFN-${\gamma}$elispot assay, intracellular staining and HLA-A2 dimer staining. Results: The frequency of peptide specific CD8+ T cell was in the range of 1 to 25 in $1.7{\times}10^5$ PBMC based on ex vivo IFN-${\gamma}$ elispot assay, demonstrating that these peptide specific CD8+ T cell responses are induced in both TB patients and PPD+ people. Short term cell lines (STCL) specific for these peptides proliferated in vitro and secreted IFN-${\gamma}$ upon antigenic stimulation in PPD+ donors. Lastly, HLA-$A^*0201$ dimer assays indicated that $PstA1_{75-83}$ specific CD8+ T cell population in PPD+ healthy donors is heterogeneous since approximately 25~33% of $PstA1_{75-83}$ specific CD8+ T cell population in PPD+ healthy donors produced IFN-${\gamma}$ upon peptide stimulation. Conclusion: Our results suggest that MHC class I restricted CD8+ T cell mediated immune responses to M. tuberculosis infection are induced in both TB patients and PPD + people; however, the CD8+ T cell population is functionally heterogeneous.
최대 지연시간을 고려한 ET 모델에서의 단일 기계 일정계획
조성아,조충호,이동훈,김채복 한국경영과학회 1998 韓國經營科學會誌 Vol.23 No.1
This paper addresses the problem of scheduling a set of jobs a common due date on a single machine. The objective is to minimize the sum of the earliness and tardiness of jobs subject to T_mas≤△ for△≥0. Properties for the MAD/T_max problem are found and the problem is shown to be NP-complete in the ordinary sense. According to the range of △. the problem can be solved in polynomial time. Also, some special cases where an optimal schedule is found in polynomial time are discussed.
미끄럼 마찰시 생성되는 고탄소강의 열변형층이 트라이볼로지 특성에 미치는 영향
이성애(Sungae Lee),조대현(Daehyun Cho),이영제(Youngze Lee) 한국트라이볼로지학회 2009 한국트라이볼로지학회 학술대회 Vol.2009 No.6
There have been many studies about white layers generated in various manufacturing processes such as hard turning, grinding, reaming. There are two different opinions on its wear characteristics. It is considered to be tribological adventage in some instance, but should decrease wear resistance because of forming micro crack and void. In this study, Ball-on-Disk type tester was used for sliding test with SUJ2 specimen due to understand the effect of white layer on friction and wear property. Surface profiler was used for investigating change of surface profiles, and transformation of micro structure was observed by FE-SEM on cross section of specimen after chemical etching.
Eun Jung Cho,Myoung Soo Park,Sahng Seop Kim,Gun Kang,Sunga Choi,이유란,장석종,Kwon Ho Lee,이상도,박진봉,전병화 대한약리학회 2011 The Korean Journal of Physiology & Pharmacology Vol.15 No.6
Ulmus davidiana var. japonica Rehder (Urticales: Ulmaceae) (UD) is a tree widespread in northeast Asia. It is traditionally used for anticancer and anti-inflammatory therapy. The present study investigated the effect of an ethanol extract of UD on vascular tension and its underlying mechanism in rats. The dried root bark of UD was ground and extracted with 80% ethanol. The prepared UD extract was used in further analysis. The effect of UD on the cell viability, vasoreactivity and hemodynamics were investigated using propidium iodide staining in cultured cells, isometric tension recording and blood pressure analysis, respectively. Low dose of UD (10∼100μg/ml) did not affect endothelial cell viability, but high dose of UD reduced cell viability. UD induced vasorelaxation in the range of 0.1∼10μg/ml with an ED50 value of 2μg/ml. UD-induced vasorelaxation was completely abolished by removal of the endothelium or by pre-treatment with L-NAME, an inhibitor of nitric oxide synthase. UD inhibited calcium influx induced by phenylephrine and high K+ and also completely abolished the effect of L-NAME. Intravenous injection of UD extracts (10∼100 mg/kg) decreased arterial and ventricular pressure in a dose-dependent manner. Moreover, UD extracts reduced the ventricular contractility (+dP/dt) in anesthetized rats. However, UD-induced hypotensive actions were minimized in L-NAME-treated rats. Taken together, out results showed that UD induced vasorelaxation and has antihypertensive properties, which may be due the activation of nitric oxide synthase in endothelium.
그라파이트 박막과 DLC 박막을 증착한 디젤 엔진용 피스톤 스커트와 실린더 블록의 마찰 특성
조대현(DaeHyun Cho),이성애(SungAe Lee),이영제(YoungZe Lee) 한국트라이볼로지학회 2008 한국트라이볼로지학회 학술대회 Vol.2008 No.6
In this study friction and wear characteristics between piston skirts and cylinder bores were investigated using the reciprocating wear tester in SAE5W40 oil condition. The relative motion between a piston skirt and a cylinder liner in a real engine is considered to reciprocation wear tester. The cylinder blocks were used as reciprocation specimens, and the piston skirt samples with graphite and DLC coatings were used as fixed pin.