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Jin‑Sung Park,Joo‑Il Kim,Hyun‑Jin Lim,Soo‑Kyung Ryu,Euna Kwon,Kang‑Min Han,Ki‑Taek Nam,Han‑Woong Lee,Byeong‑Cheol Kang 한국유전학회 2020 Genes & Genomics Vol.42 No.9
Background p19arf, primarily known as a tumor suppressor, has also been reported to play an essential role in normal development of mouse eyes. Consistently, lack of p19arf has been associated with ocular defects, but the mixed background of the knockout (KO) mouse strain used raised a concern on the accuracy of the phenotypes observed in association with the targeted gene due to genetic heterogeneity. Object We carried out a study to investigate into the efect of genetic background on the manifestation of p19arf KO associated phenotypes. Methods We characterized the phenotypes of novel p19arf KO mouse lines generated in FVB/N and C57BL/6J using a transcription activator-like efector nuclease (TALEN) system in comparison to the reported phenotypes of three other p19arf-defcient mouse lines generated using homologous recombination. Results Ninety-fve percent of FVB/N-p19arf KO mice showed ocular opacity from week 4 after birth which worsened rapidly until week 6, while such abnormality was absent in C57BL/6J-p19arf KO mice up to the age of 26 weeks. Histopathological analysis revealed retrolental masses and dysplasia in the retinal layer in FVB/N-p19arf KO mice from week 4. Besides these, both strains developed normally from birth to week 26 without increased tumorigenesis except for a subcutaneous tumor found in a C57BL/6J-p19arf KO mouse. Conclusion Our fndings demonstrated surprisingly variable manifestation of p19arf-linked phenotypes between FVB/N and C57BL/6J mice, and furthermore between our mouse lines and the established lines, indicating a critical impact of genetic background on functional study of genes using gene targeting strategies in mice.
Improved Micropropagation of Root Chicory, Cichorium intybus L. var. sativus.
Lim,Jung-Dae,Yang,Deok-Chun,Lee,Hyeon-Yong,Kim,Jong-Dai,Lee,Jin-Ha,Sung,Eun-Soo,Yu Chang-Yeon 한국자원식물학회 2004 Plant Resources Vol.7 No.1
The establishment of an efficient protocol for plant regeneration and micropropagation from leaf explant cultures of Chicory, Cichorium intybus L. var. sativus. is reported. Callus formation rate appeared 100% from explant in all growth regulators, but calli formed in the prensence of naphthaleneacetic acid (NAA) were appeared very compact and non-embryogenic state. The regenerated shoots were obtained from leaf explant cultures on solid MS medium containing different concentrations of cytokinins and auxin. The highest number of shoots (5.7) per explant and shoot growth (2.8cm) was obtained on MS medium containing 0.1 mg BAP L^-1 and 0.1 mg NAA L^-1. Indole acetic acid was the most suitable auxin for root formation among three auxins tested. 2,4-D had no effect on shoot and root formation.
Dae-Weon Lee,Il Sung Moon,강재순,Hyun Jung Choe,최재영,제연호,이시혁,Hye Rim Han,Kook-Jin Lim6,Young Ho Koh 한국응용곤충학회 2007 Journal of Asia-Pacific Entomology Vol.10 No.2
Bursaphelenchus xylophilus has been recognized as a causal pathogen of pine wilt disease (PWD). In order to identify relevant molecular biomarkers, we selected a variable region of endo-β-1,4-glucanase (β14-gcn) which was cloned from the cDNA of B. xylophilus and B. mucronatus. We then identified three novel β14-gcn isoforms: Bm-β14-gcn and Bm-β14-gcni from B. mucronatus, and Bx-β14-gcn from B. xylophilus. Bm-β14-gcn and Bx-β14-gcn were found to be identical, whereas Bm-β14-gcni was unique to B. mucronatus. These isoforms are Bursaphelenchus-specific and may be employed as molecular markers for the diagnosis of PWD. Our phylogenetic analysis showed that these β14-gcns from Bursaphelenchus species were associated most closely with fungal β14-gcns.
Park, Jinbong,Jeon, Yong-Deok,Kim, Hye-Lin,Lim, Hara,Jung, Yunu,Youn, Dong-Hyun,Jeong, Mi-Young,Kim, Hyun-Ju,Kim, Sung-Hoon,Kim, Su-Jin,Hong, Seung-Heon,Um, Jae-Young Hindawi Publishing Corporation 2013 Evidence-based Complementary and Alternative Medic Vol.2013 No.-
<P>Obesity has become a major health threat in developed countries. However, current medications for obesity are limited because of their adverse effects. Interest in natural products for the treatment of obesity is thus rapidly growing. Korean Medicine (KM) is characterized by the wide use of herbal formulas. However, the combination rule of herbal formulas in KM lacks experimental evidence. According to <I>Shennong's Classic of Materia Medica</I>, the earliest book of herbal medicine, <I>Veratrum nigrum</I> (VN) has antagonistic features against <I>Panax ginseng</I> (PG), and the PG-VN pair is strictly forbidden. In this study, we have shown the effects of PG, VN, and their combination on obesity in high-fat (HF) diet-induced obese mice and in 3T3-L1 cells. PG, VN, and PG-VN combination significantly reduced weight gain and the fat pad weight in HF diet-induced obese mice. They also significantly decreased lipid accumulation and the expressions of two major adipogenesis factors, PPAR<I><I>γ</I></I> and C/EBP<I><I>α</I></I>, in 3T3-L1 cells. In addition, the PG-VN combination had synergistic effects compared with the mixture of extracts of PG and VN on inhibition of PPAR<I><I>γ</I></I> and C/EBP<I><I>α</I></I> expressions at lower doses. These results indicate a new potential anti-obese pharmacotherapy and also provide scientific evidence supporting the usage of herbal combinations instead of mixtures in KM.</P>
( Jin Chang Moon ),( In Hee Kim ),( Seung Young Seo ),( Chang Hun Lee ),( Min Ha Kook ),( Kyung Bo Yoo ),( In Jin Cho ),( Jung Ho Lim ),( Seong Hun Kim ),( Sang Wook Kim ),( Seung Ok Lee ),( Soo Teik 대한간학회 2013 춘·추계 학술대회 (KASL) Vol.2013 No.1
Background: Oral nucleos(t)ide analogues (NA) therapy have been successfully used for suppressing circulating serum viral loads. In this study, we investigated long-term effect of oral NA therapy on the disease progression and development of hepatocellular carcinoma (HCC) in chronic hepatitis B (CHB) patients. Methods: This retrospective study included 524 naive CHB patients who had received oral NA therapy for more than 48 weeks between January 2003 and December 2012 at Chonbuk National University Hospital. Primary outcome was 5-year cumulative probability of disease progression, defined as any of cirrhosis development, decompensation with cirrhotic complications, HCC, and/or liver-related mortality. Results: Of total 524 patients, the mean age was 46.2 years, 340 (64.9%) were male, 360 (68.7%) were HBeAg positive, 188 (35.4%) had cirrhosis, 452 (86.3%)/62 (11.8%)/10 (1.9%) were Child-Pugh class A/B/C, and median follow up duration was 60 months (range 12-60). Cumulative probabilities of disease progression at 1, 2, 3, 4, 5 years were 0.2%, 1.2%, 2.3%, 7.1%, 19.3%, respectively. Cumulative probabilities of HCC at 1, 2, 3, 4, 5 years were 0.2%, 0.6%, 1.3%, 3.4%, 11.1%, respectively. In subgroup analysis among patients with cirrhosis at baseline, cumulative probabilities of disease progression at 1, 2, 3, 4, 5 years were 0.5%, 2.2%, 6.6%, 15.3%, 38.2%, and cumulative probabilities of HCC at 1, 2, 3, 4, 5 years were 0.5%, 1.6%, 3.5%, 8.0%, 26.2%, respectively. Multivariate analysis revealed that age (HR 1.03, P=0.031), body mass index (HR 1.03, P=0.032), cirrhosis (HR 2.23, P=0.035) were significant risk factors for disease progression and age (HR 1.05, P=0.022), male (HR 3.06, P=0.019), cirrhosis (HR 5.25, P=0.003) were significant risk factors for HCC. Conclusions: In the oral NA therapy era, the risks of disease progression or HCC in CHB patients were lowered than previous reports, however, it could increase according to longer duration of follow up, especially for patients with cirrhosis.
( Sung-jong Jeon ),( Su-jin Lim ) 한국미생물생명공학회(구 한국산업미생물학회) 2017 Journal of microbiology and biotechnology Vol.27 No.6
Marasmius scorodonius secretes an extracellular laccase in potato dextrose broth, and this enzyme was purified up to 206-fold using (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub> precipitation and a Hi-trap Q Sepharose column. The molecular mass of the purified laccase was estimated to be ~67 kDa by SDSPAGE. The UV/vis spectrum of the enzyme was nontypical for laccases, and metal content analysis revealed that the enzyme contains 1 mole of Fe and Zn and 2 moles of Cu per mole of protein. The optimal pH for the enzymatic activity was 3.4, 4.0, and 4.6 with 2,2`-azino-bis(3- ethylbenzothazoline-6-sulfonate) (ABTS), guaiacol, and 2,6-dimethoxy phenol as the substrate, respectively. The optimal temperature of the enzyme was 75°C with ABTS as the substrate. The enzyme was stable in the presence of some metal ions such as Ca<sup>2+</sup>, Cu<sup>2+</sup>, Ni<sup>2+</sup>, Mg<sup>2+</sup>, Mn<sup>2+</sup>, Ba<sup>2+</sup>, Co<sup>2+</sup>, and Zn<sup>2+</sup> at a low concentration (1 mM), whereas Fe<sup>2+</sup> completely inhibited the enzymatic activity. The enzymatic reaction was strongly inhibited by metal chelators and thiol compounds except for EDTA. This enzyme directly decolorized Congo red, Malachite green, Crystal violet, and Methylene green dyes at various decolorization rates of 63-90%. In the presence of 1-hydroxybenzotriazole as a redox mediator, the decolorization of Reactive orange 16 and Remazol brilliant blue R was also achieved.
Sang Bum Kim,Jin Woo Lim,Jeong Gook Seo,Jeong Ku Ha 대한정형외과학회 2016 Clinics in Orthopedic Surgery Vol.8 No.1
Many complications have been reported during or after anterior cruciate ligament (ACL) reconstruction, including infection, bleeding, tibial tunnel widening, arthrofibrosis, and graft failure. However, arterial injury has been rarely reported. This paper reports a case of an anterior tibial arterial injury during bicortical tibial drilling in arthroscopic ACL reconstruction, associated with an asymptomatic occlusion of the popliteal artery. The patient had a vague pain which led to delayed diagnosis of compartment syndrome and delayed treatment with fasciotomy. All surgeons should be aware of these rare but critical complications because the results may be disastrous like muscle necrosis as in this case.