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Baek, In-Jeoung,Jung, Ki Youn,Yon, Jung-Min,Lee, Se-Ra,Lee, Beom Jun,Yun, Young Won,Nam, Sang-Yoon Springer 2011 In vitro cellular & developmental biology. Animal Vol.47 No.8
<P>Although it has been suggested that the transcription of phospholipid hydroperoxide glutathione peroxidase (PHGPx), an essential antioxidant selenoenzyme, may be affected by the estrogen state in mammals, the direct mechanism underlying the regulation of the PHGPx gene by estrogens in mammalian tissues remains to be clearly elucidated. In this study, we evaluated the expression of the PHGPx mRNA in cultured mouse fetuses (embryonic days 8.5-10.5) exposed to 17관-estradiol (E(2); 0.1, 1, 10, 100, and 1,000 ng/ml); estrogen receptor (ER) agonists [propyl pyrazole triol (PPT, an ER관-selective ligand, 1 관l/ml) and diarylpropionitrile (DPN, an ER관-selective ligand, 1 관l/ml)]; and/or ER antagonist [ICI 182,780 (ICI, 1 관l/ml)] using a whole embryo culture system. E(2)-alone treatment significantly stimulated the expressions of both ER관 and ER관 mRNAs in all the cultured fetuses (p < 0.05), although the ER관 mRNA levels were higher than ER관 mRNA. PHGPx mRNA expression was significantly increased in all the fetuses treated with E(2) (1-1,000 ng/ml), PPT, and DPN (p < 0.05). Furthermore, pretreatment with ICI completely blocked the E(2)-induced PHGPx mRNA expression in the fetuses. In addition, the mRNA levels of cytosolic GPx, the other intracellular antioxidant selenoenzyme, did not differ significantly from the controls by an exposure to those agents. These results suggest that the PHGPx gene is regulated via an estrogen and ER signal pathway in the cultured mouse fetus.</P>
Baek, Jong Yun,Yu, Jeong Il,Park, Hee Chul,Choi, Doo Ho,Yoo, Gyu Sang,Cho, Won Kyung,Lee, Woo-Yong,Yun, Seong Hyeon,Cho, Yong Beom,Park, Yoon Ah,Kim, Hee Cheol The Korean Society for Radiation Oncology 2019 Radiation Oncology Journal Vol.37 No.2
Purpose: This study aimed to identify prognostic factors for locoregional recurrence (LRR) in pT3N0 rectal cancer patients who were treated with surgery alone and had negative resection margin including circumferential resection margin (CRM) for optimal indication of adjuvant radiotherapy. Materials and Methods: We reviewed patients with pT3N0 rectal cancer who were treated via upfront surgery and had no other adjuvant treatment from January 2003 to December 2012. In total, 122 patients who had negative resection margin including negative CRM were included in the analysis. Results: The median follow-up period after surgery was 60 months (range, 3 to 161 months). During this time, 6 patients (4.9%) experienced LRR at the anastomotic site (4 patients), and regional lymphatic area (2 patients). The estimated 5-year rates of overall survival, recurrence-free survival, and LRR-free survival were 96.7%, 84.6%, and 94.0%, respectively. Multivariate analysis showed that level of tumor ≤5 cm was a significant prognostic factor for LRR-free survival (LRRFS) (p = 0.04; hazard ratio = 7.08; 95% confidence interval, 1.06-47.30). Patients with level of tumor ≤5 cm had an estimated 5-year LRRFS of 66.8%, which was much higher than 2.3% in patients with level of tumor >5 cm. There was no significant factor for recurrence-free survival or overall survival. Conclusion: In T3N0 rectal cancer, adjuvant chemoradiotherapy should be recommended in patients with level of tumor ≤5 cm for better local control. However, in patients with pT3N0 disease, negative resection margin, and level of tumor >5 cm, adjuvant chemoradiotherapy should be carefully suggested.
Sang Gon Kim,Seonghyu Shin,Hwan Hee Bae,Jin-Seok Lee,Jung-Tae Kim,Min Jung Seo,Beom-Young Son,Jeom Ho Lee,Seong-Bum Baek 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
Understanding the response of a crop to water deficiency is the first step towards breeding drought-tolerant varieties. In this study, inbred maize (Zea mays L.) lines KS140 and KS141 were subjected to drought stress by withholding water for 10 days at the V5 or V6 leaf stage. Water-deficient plants experienced a decrease in relative leaf water content, stomatal conductance, net CO2 assimilation rate, and water use efficiency compared to well-watered plants. This was accompanied by a decrease in the relative leaf water content that resulted in severe growth retardation in KS140 and KS141. However, leaf chlorophyll content in KS140 was unchanged. To understand the proteome dynamics during the 10-day drought stress in maize leaves, comparative proteome analysis was carried out between the well-watered and water-withheld leaves. Differential expression was observed for 29 protein spots from KS140 and 14 protein spots from KS141, and these were identified using MALDI-TOF mass spectrometry. Among identified proteins, metabolism and stress related proteins were highly were increased by drought stress. This study provides a protein profile of a Korean maize inbred line during drought stress, which will be valuable for future studies of the molecular mechanisms underlying drought resistance and for development of selective breeding markers for drought tolerance in maize.
Developing PCR-Based SNP Markers for Distinguishing Korean Waxy Corn F<sub>1</sub> Hybrids
( Sang Gon Kim ),( Jin-seok Lee ),( Seonghyu Shin ),( Hwan Hee Bae ),( Jung-tae Kim ),( Beom-young Son ),( Seong-bum Baek ) 한국육종학회 2016 Plant Breeding and Biotechnology Vol.4 No.3
Single nucleotide polymorphisms (SNPs) are abundantly and evenly distributed throughout the genomes of most plant species. These markers have become popular for use in genetic research in many crops. SNP markers can be used to screen maize cultivars rapidly during the early growth stages. In this study, to develop additional SNP markers for maize, we chose 20 SNP sites per chromosome from the maizeGDB website (www.maizegdb.org) and designed primers with two base pair mismatches using Primer Designer 4 based on putative SNP sites of the B73 genome sequence. The polymerase chain reaction (PCR) products ranged from 200 to 500 bp in size, whereas no PCR product was detected when the SNP site was present in Korean waxy corn. Using nine Korean commercial F<sub>1</sub> hybrids of waxy corn, including Chalok 1, Chalok 4, Ilmichal, Eolrukchal 1, Heukjinjuchal, Hayanchal 95, Mibaekchal, Mibaek 2, and Miheukchal, we selected 16 primer sets showing clear bands or no bands. Based on cluster analysis, we confirmed that the nine Korean waxy corn hybrids could clearly be distinguished. The SNP marker sets are easy to utilize through simple PCR and agarose gel electrophoresis. These results suggest that analysis using the SNP marker set designed in this study would be faster, cheaper, and more reproducible than that using other genotyping tools, such as cleaved amplified polymorphic sequence markers, which require the use of restriction enzymes.
Baek Soo-Hyun,Yang Seung-Chul,Park Chan-Yong,Park Chul-Woo,Cho Seok-Beom,Ryu Sang-Wan 한국물리학회 2021 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.78 No.7
InGaAs/InP single-photon avalanche diode (SPAD) with small active diameter and backside microlens was fabricated and its dark count and after-pulse noises were characterized. In addition, by optimizing multiplication layer and reducing the active diameter, high photon detection efficiency (PDE) was achieved as well as suppressed dark count probability (DCP) and after-pulse probability (APP) near room temperature. The gated Geiger-mode characteristics of SPAD were investigated with the gate amplitude of 6.6 V and repetition frequency of 10 MHz. Superior single-photon detection characteristics as PDE of 20.9%, DCP of 1.02 × 10– 5 and APP of 0.8% were observed at the operation temperature of + 20 °C. It is a promising result that allows compact and low-cost detector module for quantum key distribution system with simple cooling apparatus.
Han, Sang Beom,Baek, Seung-Hee,Park, Jeong-Sook,Yang, Hee Kyung,Kim, Ji-Young,Kim, Chong-Kook,Hwang, Jeong-Min Lippincott Williams Wilkins, Inc. 2011 Cornea Vol.30 No.12
PURPOSE:: To evaluate the effect of subconjunctivally injected liposome-encapsulated tissue plasminogen activator (tPA) on the absorption rate of subconjunctival hemorrhages (SHs). METHODS:: SHs were induced in 1 eye each of 36 rabbits by subconjunctival injection of 0.05 mL of autologous blood. After 8 hours, randomized subconjunctival injections were performed: 26,000 IU/mL liposome-encapsulated tPA (0.05 mL) in 9 eyes (group A), free-form tPA (26,000 IU/mL; 0.05 mL) in 9 eyes (group B), only liposomes (0.05 mL) in 9 eyes (group C), or no injection in 9 eyes (group D). The sizes of the SHs at 8, 24, 48, 72, 96, and 120 hours after induction were measured using an image analyzer and were compared among the 4 groups. RESULTS:: Group A showed significantly more rapid absorption rates than all the other groups at 24, 48, and 72 hours and had the significantly shortest mean elapsed time for the complete resorption of SHs. The tPA activity in ocular tissue except conjunctiva and plasma were negligible beyond 24 hours after SH induction with both forms of tPA. In the conjunctiva, the tPA activity was significantly prolonged in the liposome-encapsulated tPA group than in the free tPA group. CONCLUSIONS:: Subconjunctival injection of liposome-encapsulated tPA seems to enhance SH absorption in rabbits, especially during the early stages with minimal systemic and ocular absorption.