대나무 분말의 항산화력과 돼지의 면역 활성에 미치는 영향

송윤오 ( Yun O Song ),추교문 ( Gyo Moon Chu ),장선희 ( Sun Hee Jang ),구애진 ( Ae Jin Goo ),고응규 ( Yeoung Gyu Ko ),하지희 ( Ji Hee Ha ),이재형 ( Jae Young Lee ),강석남 ( Suk Nam Kang ),송영민 ( Young Min Song ),조재현 ( Jae Hyeo 한국동물위생학회 2014 韓國家畜衛生學會誌 Vol.37 No.2

본 연구는 대나무 분말의 항산화 활성이 비육돈에서 면역조절 효과에 미치는 영향을 구명하기 위해 실시하였으며, 이를 위해 대나무 분말 추출물의 항산화능을 검정하기 위하여, 총페놀 함량, 플라보노이드함량 및 DHHP, ABST, hydroxyl radical 등의 소거활성능을 알아보았다. 대나무 추출물의 항산화능은 높게 나타났으며, DPPH와 ABTS, HRSA 라디칼 소거능은 12∼21%였으며, 추출물의 유효성분으로 총페놀 함량은 171 mg/g이었으며, 플라보노이드 함량은 127.mg/g으로 나타났다. 다음으로 대나무 분말이 함유된 배합사료 급여시 돼지의 면역력에 미치는 영향을 알아보았다. 처리구에는 일반사료를 급여하였고, 시험구는 일반사료에 대나무분말을 1.0%, 2.0% 및 3.0%을 첨가하여 급여하였다. 대나무분말 첨가수준에 따라 혈액의 혈구계수 중 백혈구, 적혈구, 헤마토크리트 함량에서 유의적인 수준의 차이를 나타내지 않았다. 하지만 헤모글로빈 함량이 무처리 대조구에 비교하여 증가하였으며 특히 2∼3%의 처리구에서 헤모글로빈 함량은 높게 나타났다. 혈액 내 총단백질, 혈중알부민의 농도를 분석한 결과 처리구와 무처리구간에 유의적인 차이를 나타내지 않았다. AST, ALT 함량은 대나무 분말 처리군에서 무처리군보다 약간 높게 나타나지만 정상적인 범위내에서의 변화를 보였다. immunoglobulin에서 IgA와 IgG 농도는 무처리구에 비교하여 대나무 분말 처리구에서 높게 나타나고, IgA는 처리구 간의 차이가 나타나지 않았지만 IgG 농도에서는 대나무 추출물 2%, 3% 처리구가 1% 처리구에 비해 높은 수준을 나타냈다. TNF-α의 경우 무첨가구와 첨가구 사이의 유의적인 차이가 없었지만, 대나무 분말 첨가구에서 interferon-γ의 함량은 모든 첨가구에서 무처리 군보다 유의적으로 높게 나타났다. 특히 2%, 3% 처리구가 1%의 처리구 보다도 높은 interferon-γ의 함량을 나타내었다. 이는 대나무의 페놀, 또는 플라보노이드 성분에 기초한 높은 항산화력에 의하거나, 또는 대나무에 포함된 돼지 초기 면역반응을 활성화시킬 수 있는 물질에 의한 면역활성 증강에 따른 것으로 사료된다. 결론적으로 대나무 분말의 높은 항산화력은 돼지 면역력 향상에 기여하였으며, 특히 2∼3% 대나무 분말 첨가 사료는 돼지 생리활성을 크게 증강시키는 것으로 사료된다. The present study was designed to explore the antioxidant effect of Bamboo powder and its immunoreactivity in pigs. We investigated the functional properties of Bamboo extracts by means of measuring the contents of total polyphenols and flavonoid as well as determining ABST, DPPH radical scavenging activity, and hydroxyl radical scavenging activity and anticancer activity. The total phenolic compound and flavonoids contents of Bamboo extracts were 171.25 mg/g and 127.5 mg/g, respectively. The DPPH radical, hydroxyl radical, ABST radical scavenging activity of Bamboo extracts were 17.3%, 12.5% and 21.5%, respectively. Evidenced by MTT and cell cycle assay, Bamboo dose-dependently inhibited the cell proliferation and induced G0/G1-phase arrest in CHO cells at concentrations of 100, 250, and 500 μg/ml Bamboo extracts. More than 80% of apoptotic cells were observed by staining with annexin V in 500 μg/ml Bamboo-treated CHO cells, indicating that Bamboo had potent anticancer activities. Next, to investigate the effect of Bamboo on cytokine, immunoglobulin concentration, and blood compositions, flatting pigs were fed with Bamboo powder for 38 days. Flatting pigs were divided into 4 groups; basal diet (control), basal diet supplemented with 1% Bamboo powder (T1), 2% Bamboo powder (T2), and 3% Bamboo powder (T3). The level of hemoglobin increased in the all Bamboo-fed groups compared with the normal control group. In particular, platelet levels in the all Bamboo-treated groups increased by approximately 90% compared with the levels from pig on a normal control. Serum levels of immunoglobulins (IgG, IgA) in the pigs fed Bamboo powder were modestly increased, and the interferon-γ level also was strongly increased in 2% or 3% Bamboo-fed groups compared with the levels in control groups. Together, these results demonstrated that Bamboo extracts had an effective capacity of scavenging for ABTS, DPPH, and hydroxyl radicals and showed correlation with potent phenol and flavonoid contents, thus suggesting its antioxidant potential. Moreover, administration of Bamboo in 2∼3% improved blood parameters and platelets, and especially immunity-related ones such as IgG, IgA, and interferon- γ, leading to be potential feed additives in flatting pigs.

In vitro inhibitory effect of piperlonguminine isolated from Piper longum on human cytochrome P450 1A2

Song, Min,Hwang, Jae Yun,Lee, Min Young,Jee, Jun-Goo,Lee, You Mie,Bae, Jong-Sup,Kim, Jeong Ah,Lee, Seung Ho,Lee, Sangkyu 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.8

Piperlonguminine (PL), a major alkaloid isolated from Piper longum fruits, shows several biological activities including anti-tumor, anti-hyperlipidemic and anti-inflammatory effects. Although there have been studies of the biological effects of PL, the potential drug-interaction effect of PL following evaluation of inhibitory effects of cytochrome P450 (CYP) activities was not investigated. Here, to investigate the inhibitory effects of PL on the activities of CYP isoforms, CYP inhibition assays were conducted using a cocktail of probe substrates in pooled human liver microsome (HLMs) and human recombinant cDNA-expressed CYP. PL strongly inhibited CYP1A2-mediated phenacetin O-deethylation with an $IC_{50}$ value of $8.8{\mu}M$, as NADPH-independent inhibition, while other CYPs were not significantly inhibited. A Lineweaver-Burk plot resulted in the inhibition mechanism of PL being divided into two different modes, reversible competitive inhibition in a low concentration range of $0-16{\mu}M$ with a $K_i$ value of $1.39{\mu}M$ and uncompetitive inhibitory behavior at a high concentration range of $16-40{\mu}M$. In addition, PL only decreased CYP 1A2-catalyzed phenacetin O-deethylase activity with $IC_{50}$ values of $10.0{\mu}M$ in human recombinant cDNA-expressed 1A2, not 1A1. Overall, this is the first investigation of potential herb-drug interactions associated with PL conducted by identifying the competitive inhibitory effects of PL on CYP1A2 in HLMs.

Hydride bed control: Understanding of hydride bed using tank efflux

Yun, Sei-Hun,Chang, Min Ho,Kang, Hyun-Goo,Chung, Dongyou,Lee, Hyeon Gon,Jung, Ki Jung,Chung, Hongsuk,Song, Kyu-Min,Lee, Euy Soo,Lee, In-Beum,Park, Byeong Eon Elsevier 2017 International journal of hydrogen energy Vol.42 No.12

<P><B>Abstract</B></P> <P>Control of dehydriding (desorption of hydrogen or hydrogen isotope) rate from a hydride bed in fusion fuel cycle is one important design point to estimate a real supplying amount of hydrogen from the hydride bed. In a real system tens of batch-type hydride beds are to be utilized for supplying a certain amount of hydrogen isotope at the same time. A study on efflux time from a hydraulic water tank was applied as a fundamental similarity test of the gas fueling system. As a result, liquid efflux from a tank shows a similar behavior with the desorption pattern of the depleted uranium hydride bed system. As much important as keeping a hydride buffer vessel pressure in a hydride bed system, similar tendency was studied in the tank efflux system; i.e., to keep the secondary vessel height there needs a certain amount of liquid flow from the upper tank and the tank height difference. From one tank with connected another tank flow with understanding of tank efflux model a complicated multi-tanks behavior could be understood by simulating its complex efflux characteristics, and it is likely to be applied to the multi-hydride beds system.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Understanding of dehydriding from a hydride bed using tank efflux. </LI> <LI> Similar pattern shows between depleted uranium dehydriding and liquid efflux. </LI> <LI> Supplying a stable fueling rate is of importance to keep the secondary buffer level. </LI> <LI> Dehydriding behavior in multi-hydride bed was studied using multi-tank efflux. </LI> <LI> Multi-efflux seems to be substituted for control study on multi-dehydriding system. </LI> </UL> </P>

Risk factors and outcomes for persistent candidaemia (초)

Yun Tae Chae,Sang Hoon Han,Bum Sik Chin,So Youn Shin,Chang Oh Kim,Hee Kyung Choi,Ji Hyeon Baek,Jun Yong Choi,Sung Joon Jin,Young Goo Song,June Myung Kim 대한내과학회 2009 대한내과학회지 Vol.77 No.4S

Expressional Variation of Endoplasmic Reticulum Stress Signaling Factors in Mouse Tissues by Aging

Song-Yi Han,Cho-Yi Jin,Kisang Kwon,Eun Young Yun,Tae Won Goo,Seungwhan Kim,O-Yu Kwon(권오유) 대한의생명과학회 2010 Biomedical Science Letters Vol.16 No.1

The endoplasmic reticulum (ER) is a multifunctional intercellular organelle in which several posttranslational modification steps occurred such as protein folding, lipid biosynthesis, calcium storage and release. Perturbations that disrupt ER homeostasis lead to the misfolding of proteins in the ER lumen and up-regulation of ER signaling pathway called the unfolded protein response (UPR). Here, we have demonstrated that ageing changes the expression of ER chaperone and associated ER membrane kinases of IRE1, ATF6 and PERK.

Risk factors and outcomes for persistent candidaemia

Yun Tae Chae,Sang Hoon Han,Bum Sik Chin,So Youn Shin,Chang Oh Kim,Hee Kyung Choi,Ji Hyeon Baek,Jun Yong Choi,Sung Joon Jin,Young Goo Song,June Myung Kim 대한내과학회 2009 대한내과학회 추계학술대회 Vol.2009 No.-

Production of Recombinant Human Keratinocyte Growth Factor from Bombyx mori (Lepidopera: Bombycidae) Bm5 Cells

Song-Yi Han(한송이),Cho-Yi Jin(진초이),Kisang Kwon(권기상),Eun Young Yun(윤은영),Tae Won Goo(구태원),Seung-Whan Kim(김승환),Jong-Soon Choi(최종순),Kweon Yu(유권),O-Yu Kwon(권오유) 한국생명과학회 2011 생명과학회지 Vol.21 No.6

인간형 재조합단백질 각질세포 성장인자를 안정적으로 생산하는 누에 배양 세포(Bm5-hKGF cell)을 만들었다, 이 세포에서 분비되어 배지에 포함된 양은 15-20 ng/㎖ 정도였다. Bm5-hKGF cell에 누에의 PDI를 함께 발현시키면 세포외 분비량이 2배 증가하였다. Wound healing migration assay 결과 누에세포에서 생산된 인간형 재조합단백질 각질세포 성장인자는 세포생장을 촉진하는 활성을 가지고 있었다. 본 실험의 결과는 누에배양세포를 사용하여 저비용으로 양질의 인간형 재조합단백질을 대량생산 할 수 있는 것을 기대한다. Using silkworm Bombyx mori Bm5 cells, we established a stable cell line expressing the human keratinocyte growth factor (hKGF), named by the Bm5-hKGF cell, in which the protein hKGF is synthesized in the cell and secreted in the cell culture supernatant (CCS) at approximately 15-20 ng/㎖. When the Bm5-hKGF cell was co-expressed with B. mori protein disulfide isomerase (bPDI) cDNA, its secretion increased by about two times the original amount. Through wound healing migration assay, it was demonstrated that the secreted hKGF included in the CCS has a very powerful biological activity of keratinocyte proliferation. We expect to produce useful human recombinant proteins from silkworm cultured cells in large quantities at low prices.

일라이트 폴리타입: 그 특성과 단층 활동연대 결정에의 활용

송윤구,Song, Yun-Goo 대한자원환경지질학회 2012 자원환경지질 Vol.45 No.2

일라이트에서 적층방식을 달리하여 나타나는 폴리타입은 대부분 1M과 $2M_1$이며, 적층 Disordering에 따른 $1M_d$ 또한 흔히 관찰된다. 본 해설에서는 일라이트의 폴리타입을 구분하고 정량적으로 해석할 수 있는 광물학적 이론적 근거와 방법을 소개하고, 소규모 열수환경에 준하는 단층대 내 생성시기 및 조건을 달리하는 일라이트 폴리타입 혼합물에의 적용을 통한 단층 활동 및 재활동연대 결정에의 활용 가능성을 검토하였다. 단층연대해석의 정확도와 신빙도를 높이기 위해서는 단층암 대상시료 내 일라이트 폴리타입의 생성환경에 대한 정보, 동정 및 광물학적 특성에 대한 규명이 이루어져야 한다. 또한 WILDFIRE(C)시뮬레이션을 이용한 정량분석에서, $1M_d$ 폴리타입의 Disordering 정도 및 I/S 광물의 팽창도에 기인한 (hkl) 회절선들의 Broadening 효과 등 일라이트 폴리타입 정량분석법의 오차요인을 최소화시키는 하기 방법을 고려해야 할 것이다. The 1M and $2M_1$ stacking sequences are the most frequently encountered in the illite species among the possible 6 polytypes. The $1M_d$, derived from the 1M polytype which exhibits a variable degree of disorder in the stacking sequence, is also observed in illite samples. In this paper, the author introduces and reviews the theoretical background of the quantitative analysis method of illite polytypes, and considers the possibility to determine the fault age and its reactivation age using K/Ar age-dating based on the quantification of illite polytypes in the fault system. For the increase of the accuracy and precision of the illite age analysis method, the occurrence, identification, and mineralogical characterization of illite polytypes should be defined in detail. The broadening effect of (hkl) reflections, due to disordering of 1M polytype and the presence of I/S minerals with expandability, are also considered as the main parameters controlling the quantification of illite polytypes using the WILDFIRE(C)simulation.

A Multi-Layer ATM Restroration Architecture and Algorithms

kim,Yun-Bae,Lee,Heesang,Kim,Sang-Baeg,Song,Hae-Goo,Chung,Jibok,Chung,Sung-Jin 성균관대학교 1998 학술회의지원논문목록집 Vol.1998 No.-

In this study, we propose a multi-layer restoration architecture for ATM networks. It consists of three restoration layers. The first restoration layer is based on a preplanned VP restoration scheme. In this scheme, the routes of alternate VPs are prepared by an optimization model that considers network-wide resource utilization and rerouting costs. The second restoration layer is based on a preplanned VC rerouting optimization. In this study, we suggest a "preplanned" VC rerouting optimization to avoid contention between VP restoration and VC restoration on the same network resources. The third restoration layer is based on a new dynamic VP restoration procedure. Some computational experiments have shown the suggested multi-layer restoration architecture and the related algorithms are feasible to implement and effective for the survivable ATM network planning.

미세변화신증에서 우측 대퇴 정맥 삽관에 의한 총장골정맥협착증과 동반된 심부정맥혈전증 및 폐색전증 1예

최윤아 ( Yun Ah Choi ),이승우 ( Seoung Woo Lee ),김정구 ( Jeong Goo Kim ),이승원 ( Seung Won Lee ),박근호 ( Geun Ho Park ),송준호 ( Joon Ho Song ),김문재 ( Moon Jae Kim ) 대한내과학회 2006 대한내과학회지 Vol.70 No.2S