신부전증과 폐에 Pseudolyrmphorma를 동만한 원발성 Sjogren 증후군 : Cyclophosphamide와 Prednisolone에 의한 성공적인 치료

강민규,구영선,황평주,김종학,나기량,서광선,이강욱,신영태 대한신장학회 2000 대한신장학회잡지 Vol.19 No.4

전신성 홍반성 낭창의 임상적 고찰

신영태,김종학,장윤경,양종오,구영선,강민규,황평주,나기량,이강욱,서광선 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

The epidemiology, diagnostic criteria, clinical features, symptoms and signs laboratory findings, kidney pathology, and clinicopathologic correlation of systemic lupus erythematosus(SLE) were analyzed. The 63 patients studied were managed at the Department of Internal medicine, CNUH, from January 1983 to December 1997. Kidney biopsy was performed in 53 patients out of 63 patients with SLE. The results were as follows: 1. The ratio of male to female was 1:26. They were 12 to 71 years old and mean age was 32.1 years old. The peak age incidence was 4th decades(30%). 2. The most frequent chief complaint on admission was generalized edema. Most patients complain two or more symptoms. 3. Immunologic and renal disorders were the most frequently observed in the ARA criteria of SLE. And the positive ANA, hematologic disorder, malar rash, and arthritis, were observed in order of frequency. 4. Among the 53 patients with renal biopsy, 30 patients revealed class Ⅳ lupus nephritis(56%), class II in 12 patients(23%), class V in 8 patients(15%) and class III in 2 patients(6%). 5. The cases of lupus nephritis represented as nephrotic syndrome were high in the class IV with 68% and class V with 86%. 6. Of 30 patients who can be followed up, 5 patients resulted in death(17 % of mortality). They "were 14 to 57 years old and mean age was 28 years old. Follow up duration were from 1 month to 6 years and 3 months, and mean duration was 1 year and 1 month, but 3 cases 7. The causes of death were as follows: Two cases were due to seizure and coma resulted from CNS involvement. One was sepsis due to miliary the & pneumonia. One was dilated cardiomyopathy and heart failure due to cardiac ac involvement. And the other one was sepsis and acute renal failure resulted from cellulitis. 8. Prednisolone was used in all patients basically, and methyl-prednisolone pulse therapy, antimalarials and alkylating agents were used in some cases. In the cases of combined therapy, the activity of SLE was well controlled.

혈청 HBsAg 양성인 사구체신염의 임상상 및 신조직 소견

신영태,구영선,강민규,황평주,김종학,이강욱,서광선 충남대학교 의과대학 지역사회의학연구소 1999 충남의대잡지 Vol.26 No.1

Hepatitis-B-associated glomerulonephritis is one of the immunologically mediated disorders associated with chronic Hepatitis B virus(HBV) infection. The frequency of HBsAg infection has been high in East Asia and it is one of the most important etiologic factors of the secondary glomerulonephritis. We reviewed the clinical and pathologic features of 29 patients with glomerulonephritis and HBs antigenemia, who were admitted to Chungnam National University Hospital from January 1988 to Febuary 1999. The results were as follows : 1) The average age of the patients was 33.8±10.4(mean±SD) and male to female ratio was 3.8. Proteinuria was present in 27 patients(93%), hematuria in 20 patients(69%) and hypertension in 17 patients(59%). Two patients(7%) manifested with renal insufficiency and 8 patients(28%) with nephrotic syndrome. 2) Renal pathology revealed membranous nephropathy(41%), membranoproliferative glomerulonephritis(21%), mesangial proliferative glomeulonephritis(21%), minimal change nephritis (14%) and focal segmental glomerulonephritis(3%). 3) The pathologic findings of liver were chronic active hepatitis(68%), chronic persistent hepatitis(38%) and liver cirrhosis(8%). 4) During the follow-up period(average:49.4 months), 7 patients(68%) presented persistent proteinuria, 5 patients(21%) progressed to renal failure and 2 patients(8%) resolved clinically. 5) The treatment was done with prednisolone to 3 patients, and with alpha-interferon to 3 patients. One patient treated with prednisolone resolved clinically, but this resolution did not coincide with disappearance of the hepatitis B antigens.

뇌동맥류 환자의 임상분석

강삼석,박중욱,류재광,신동철,김봉환 全南大醫大 1983 전남의대학술지 Vol.20 No.1

Chitinase를 생산하는 Pseudomonas sp. YS-542의 분리 및 특성

강신욱,이준우,방광웅,최희상 慶北專門大學(영주경상전문대학) 2002 慶北專門大學 論文集 Vol.21 No.-

A chitinase-producing bacterium was isolated from soil by selective enrichmentment culture medium. The bacterium was identified as Pseudomonas sp. based on the data obtained from its morphological, cultural and biochemical characteristics. The isolated strain was named Pseudomonas sp. YS-542. The medium for the production of chitinase was consisted of colloidal chitin 1.0%, tryptone 1.0%, MgS04·7H2O 0.2%, K2HP04 0.1%. The optimum cultural temperature, initial pH and time for the best production of chitinase were 25℃, 48hrs and pH 8.0, respectively.

Optode형 glucose 센서의 개발

강신원,고광락,이수미,김수진 경북대학교 센서기술연구소 1997 연차보고서 Vol.1997 No.-

인간의 미각은 단맛 (甘味, sweet taste), 신맛 (酸味, sour taste), 짠맛(鹽味, saline taste), 쓴맛 (苦味, bitter taste)의 4가지 기본요소와 우마미 (Umami)^1)로 이루어져 있다. 본 연구에서는 그 중 글루코오스를 감지할 수 있는 당도 (糖度)센서인 초보적인 미각센서의 개발을 위하여 광섬유를 이용한 optode형의 센서소자를 제작하여 glucose의 농도 1×10 exp (-7) ∼ 1×10 exp (-2) M의 범위에 대해 측정하였다. 본 센서의 측정원리는 광섬유를 통한 광의 전달시 감지막의 글루코오스에 대한 반응에 따른 흡광 특성에 의해 일어나는 출력광의 감소 즉, 소산파의 흡수를 측정하는 원리를 이용하였다. The taste is composed of five fundamental tastes, sweet, sour, saline, bitter taste and umami. This research purposed to develope a glucose sensor, sweet taste sensor. The glucose sensor device used five kinds of dye/PMMA polymer sensing membranes containing five dyes. All experiment processor is based on the pH 7.4, 0.05 M tris-HCl buffer solution and glucose concentration from 1×10 exp (-7) to 1×10 exp (-2) M range. The fiber optode of the sensor device is made of skipped cladding in the fiber's middle site and then coated by dye/PMMA membrane. The principle of the sensor is based on the absorbance change of the evanescent wave of the sensing membrane according to glucose concentration change. In this experiments, we made a response pattern of the glucose sensor system using the evanescent wave.

포장재질을 따른 김치의 저장성 Ⅱ

강미정,윤광섭,신승렬,김광수 대구효성가톨릭대학교 식품과학연구소 1997 식품과학지 Vol.9 No.-

수출용 김치에 주로 사용되고 있는 유리병을 대상으로 유통과정 중 상품성 유지와 더불어 저장성 향상을 도모하고자 포장방법에 따른 저장성 효과를 살펴보고자 하였다. 실험에 사용한 김치는 양념버무리기를 한후 저온에서 24시간 숙성시켜 어느 정도의 탄산가스를 제거한 다음 무포장, 밀폐 포장, 투과성 포장을 하였다. 각각의 포장방법은 0.01, 0.06, 0.08, 0.1㎜ PE film으로 내부포장한 뒤 10℃에서 28일간 저장하면서 pH 및 산도, 색도의 변화와 관능적 품질변화를 측정하였다. pH의 변화를 측정한 결과, 밀폐 포장과 투과성 포장에 대해 전구간이 저장 7일째까지 급격히 감소하던 pH는 이후 완만한 감소를 나타내었으며, 0.06㎜ 처리구의 경우 pH 감소가 낮고, 상미한계점이 타처리구보다 연장되는 결과를 나타내었다. 산도의 경우 0.01, 0.06㎜로 밀폐포장처리구에서 저장 28일동안 산도의 증가가 낮게 나타났고, 투과성 포장시에는 0.06, 0.08㎜처리구에서 산도 증가가 완만하였으며, 상미한계점이 연장되는 양상을 나타내었다. 또한 포장방법에 상관없이 숙성시료를 사용함에 따라 탄산가스에 의한 내부 필림의 팽창과 김치국물의 누출 현상 역시 적은 것이 과찰되었다. 색도 측정시 L, a, b값이 저장기간이 경과됨에 따라 모두 증가하는 추세로 이런 경향은 밀폐 포장과 투과성 포장간에 큰 차이가 없는 것으로 나타났으며 0.06㎜ 처리시 색도 증가가 타처리구에 비해 완만하였다. 관능검사 결과, 저장기간이 길어질수록 타처리구에 비해 0.06㎜ 처리구에서 시원한 맛(탄산미), 신맛, 조직감, 종합적인 기호도가 모두 높은 것으로 나타났으며, 포장방법간에 큰 차이는 없었으나 저장 21일까지는 밀폐포장보다 투과성 포장시 기호적 품질이 높은 것으로 보인다. 이상의 결과로 밀폐 포장하거나 투과성 포장하는 것은 일반 유리병포장보다 저장성 효과가 크며 밀폐 포장의 경우 film 두께가 다소 얇은 0.01, 0.06㎜에서 그 효과가 현저히 높았고 투과성 포장의 경우 film 두께가 다소 두터운 0.06, 0.08㎜에서 저장성 연장을 기대할 수 있다 특히 0.06㎜ film은 밀폐 포장, 투과성 포장 모두에서 높은 저장성 연장효과와 더불어 탄산가스에 의한 팽창방지를 완화시킬 것으로 사료된다. The effects of various packaging methods in bottle on the quality and shelf-life of kimchi were investigated. Kimchi was packaged in different methods such as air-tight and permeable packaging used PE film with various thickness(0.01, 0.06, 0.08 and 0.1㎜). Also, kimchi was measured the changes in pH, titratable acidity, color, taste and texture by sensory and instrumental evaluation during fermentation at 10℃. The sharp decrease in pH of both air-tight and permeable packaging showed up to 7 days of storage. Especially, the decrease in pH by 0.06㎜ of PE film was lower than the others and the increases in acidity by air-tight samples with 0.01 and 0.06㎜ PE film were also lower. Color index(L, b/a) of crushed kimchi juice increased during storage regardless of packaging methods such as air-tight and permeable packaging. In sensory test, the scores of fresh taste, sour taste, texture and preference were higher in sample with 0.06㎜ of PE film than in the others. The quality of kimchi was not significantly affacted by the packaging methods up to 21 days of storage. These results indicate that air-tight and permeable packaging with 0.06㎜ of PE film were affected the quality and shelf-life of kimchi.

자발적 초점성분절성사구체경화증 Mouse 모델에서 TGF-β및 MCP-1 유전자발현에 미치는 Renin Angiotensin System 의 영향에 관한 연구

신영태,서광선,이강욱 충남대학교 의과대학 지역사회의학연구소 1999 충남의대잡지 Vol.26 No.1

The role of angiotensin Ⅱ in the pathogenesis of the progressive renal injury process has been studied in many human renal diseases and animal renal injury models. The inhibition of angiotensin Ⅱ also has been reported to be effective to attenuate renal injury process in many human renal diseases and animal models. The activation of TGF-β and MCP-1 gene is thought to be related with renal injury process. A mutant strain FGS/Nga/Kist mouse is reported to develop focal and segmental glomerulosis, spontaneously. In order to evaluate renal pathology, MCP-1 and TGF-β gene expressions and the effect of renin-angiotensin system inhibition, 8 FGS/Nga/Kist mice were treated by angiotensin converting enzyme inhibitor, enalapril (150 mg/L in drinking water) for 12 weeks. Another non-treated, agematched 8 FGS/Nga/Kist mice and 6 nomal CBA/Nga mice were also included in this study. The urinary protein excrtion, magnitude of glomerular sclerosis, and renal express of MCP-1 and TGF-β were evaluated by uristick, light microscopic examination and competitive RT-PCR, respectively. CBA/Nga mice did not show significant proteinuria. However, urinary protein scores of enalapril-treated FgS/Nga/Kist mice was significantly lower than that of non-treated FGS/Nga/Kist mice (2.0±0.3, 4.1±0.5, respectively, p <0.05). Focal and segmental glomerular scierosis index of enalapril treated FGS/Nga/Kist mice was significantly lower than that of non-treated FGS/Nga/Kist mice (120±13, 189±15, respectively, p < 0.05). The level of renal TGF-β gene expression of enalapril treated FGS/Nga/Kist was significantly higher than that of CBA/Nga mice (4.7±1.3, 1.0±0.3, respentively, p< 0.05). But, enalapril treated FGs/Nga/Kist mice showed significantly lower level of TGF-β expression than that of non-treated FGS/Nga/Kist mice(p<0.05). MCP-1 gene expression of untreated FGS/Nga/Kist mice was significantly lower than that of CBA/Nga mice. However, enalapril treated FGS/Nga/Kist mice showed markedly increased level of MCP-1 gene expression compared to the untreated FGS/Nga/Kist mice(9.8±1.8, 3.6±1.2, respectively, p<0.05). With the above result, we speculate that angiotensin converting enzyme ingibition attenuates the renal injury process in this model and the regulation pathway of MCP-1 by renin-angiotensin system may be different from other animal renal injury models.

자연 교과 지도에 대한 초등 교사의 인식

김광명,신순임,강태완,이대형,장병기 춘천교육대학교 과학교육연구소 1995 과학교육연구 Vol.18 No.-

우라늄 이온(UO_2^2+) - 초분자 화학센서

고광락,McStay, D.,엄년식,강신원 경북대학교 센서기술연구소 1997 연차보고서 Vol.1997 No.-

본 연구는 표면 플라즈몬 공명 현상을 이용한 우라늄 (UO_2^2+) - 초분자 화학센서시스템의 기초적 개발에 대한 것으로, 크게 표면 플라즈몬 공명형 센서 시스템의 간단한 구축과 구성된 센서 시스템의 기초 특성 및 우라늄 이온 감지 특성 평가에 대한내용으로 수행되었다. 특히 우라닐 이온 감지 특성에서, 우라닐 이온의 선택적 감지물질인 수용성 calix[6] arene이 함유된 감지막을 사용하여 수용액 상태에서 우라닐 이온의 감지정도를 측정해 본 결과 calix[6] arene이 함유되지 않은 감지막에 비해 플라즈몬 공명각의 변화량이 약 5.5 배 커짐을 볼 수 있었고, 10^-7 ∼ 10^-11 M의 저농도 우라닐 이온을 함유한 수용액을 농도별로 제조하여 공명특성을 측정한 결과 decade 농도당 공명각 변화량이 0.145°로 감도가 비교적 높았으며 빠른 응답특성도 함께 볼 수 있었다. This project was basic studies for the constructing the uranium ion - supramolecular chemosensor system using SPR (surface plasmon resonance) phenomenon. We constructed a simple SPR sensor system and measured the characterization of the sensing chip and uranyl ion detecting with this system. On uranyl ion sensing In solution state, especially, the use of sensing matrix interface with very well-known receptor for uranyl ion (calix[6]arene) and polymer composite matrix amplified resonance angle shift of the constructed SPR system. This amplified signal is about 5.5 times greater than the case of using simple polymeric matrix without calix[6]arene as a sensing interface. Also the linear resonance angle shift was observed in the range of very low uranyl ion concentration from 10 to 10 M. The sensitivity (resonance angle shift per uranyl concentration decade) was relatively high as 0.145° and the response time was similar to almost measurement moment.