요부수핵탈출증에서 Thermography의 진단적 가치

신현택,신승우,송재철,정석희,이종수,김성수,신현대 경희대학교 동서의학연구소 2001 東西醫學硏究所 論文集 Vol.2000 No.-

Objectives : Lumbar radiculopathy is a common disease in oriental rehabilitation medicine. It can be diagnosed by physical examination, plain X-ray, Myelography, CT, MRI, EMG etc. But those methods are not useful in observing the clinical procedure. Objectvie evaluating the clinical procedure is very important in treatment of lumbar radiculopathy. Mehthods : We studied the clinical efficacy of thermography in lumbar radiculopathy during conservative managements. We studied 36 patients with lumbar radiculopathy convinced by MRI. The had low back pain and radiating pain on one side. Thermography had taken before an after 4 weeks treatments, and compared with clinical procedure Results and conclusions : Thermography is not pertinent as a primary diagnostic method in lumbar radiculopathy, but useful in observing the clinical procedure. And it can be recommended as a objective evaluation for lumbar radiculopathy.

비만증 환자에서 한약제에 의한 체중 감소효과

Oh, Seung-Joon,Jeong, In-Kyung,Kim, Young-Seol,Choi, Young-Kil,Paeng, Jeong-Ryung,Bae, Jung-Hwan,Shin, Hyun-Dae 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Seung-Joon Oh, In-Kyung Jeong, Young-Seol Kim, Young-Kil Choi, Jeong-Ryung Paeng¹, Jung-Hwan Bae and Hyun-Dae Shin²Department of internal Medicine, College Medicine, Endocrine Research Institute¹. Department of Rehabilitation, College of Oriental Medicine², Seoul, korea. Bady Fat Reduction Effects of Red Ginseng Compound Preparation on the Patients with Obesity. Proceedings of International Symposium on East-West Medicine, Seoul. 244-254, 1999.-Obesity can be defined as a metabolic disease due to an increased state of fat tissues caused by an imbalance of calorie intake and use. Recently, in Korea by improvement and westernization of food intake, along with decrease in exercise activities, the prevalence of obesity has increased greatly. Our objectives were to study stability and effects of decrease in body fat by administering red ginseng compound preparation (known to have body fat decreasing effects in laboratory animals) to obesity patients on low calorie diets. Changes in weight and body fat were measured while carrying out calorie-restricted diets on patients for 4 weeks, then administering red ginseng compound preparation for another 4 weeks. The patients were 20 people whose BMI were 25kg/㎡ or over and whose percent body fat was also 30% or over when tested by bioelectrical conductivity. 1. Changes in weight were from 70.04kg(base line) to 67.43kg(after taking red ginseng compound preparation). 2. In similar sense, BMI decreased from 27.12kg/㎡(base line) to 26.56kg/㎡(after dieting), and further to 26.01kg/㎡ (after taking red ginseng compound preparation). The BMI seemed to decrease significantly compared to the baseline after the use of red ginseng compound. 3. Waist hip ratio was changed from 0.8858(base line) to 0.8728, but it was statistically insignificant. 4. The percent body fat was 35,16%(base line), 33.87%(after dieting), and 31.68%(after taking red ginseng compound preparation). 5. Complete blood cell count and blood chemisrty remained unaffected by the administration. 6. In concern to endocrinologic studies, T3 decreased from 118.7 to 98.2ng/dL, and T4 increased from 8.8 to 9.2㎕/ dL. Epinephrine showed a tendency to decreased from 0.27 to 0.25 ng/mL, and norepinephrine increased from 0.39 to 0.44ng/mL. 7. Leptin was not changed. 8. Some patients complained adverse effects; constipation(5 patients, may be due to diet therapy), fatigue (2 patients), pruritus(2 patients), flushing(s patients), dizziness(3 patients) and epigastric discomfort(2 patients). However their symptoms were mild, so medication did not stopped. In conclusion, loss of weight without significant side effects was observed during low calorie diet and red ginseng compound preparation administration. This is thought to be in relation to sympathetic nerve system rather than adrenal gland. Also, further long0term studies should be required, since the observed results were based on short-term changes in weight.

운동실조를 동반하지 않은 급성외안근마비 1례

신대수,이승회,이한샘,박성욱,박현영,장혁,김요식,조광호 圓光大學校 醫科學硏究所 2004 圓光醫科學 Vol.19 No.2

Quinacrine 형광을 이용한 토마토 뿌리조직 마이크로솜의 수소이온이동 활성측정

신대섭,조광현,김영기 충북대학교 한국과학재단 지정 첨단원예기술개발 연구센터 2002 연구보고서 Vol.6 No.-

Quinacrine은 수소이온 농도변화에 민감한 형광 probe로서 양성자와 결합하지 않은 형광형이나, 양성자와 결합한 비형광형으로 존재한다. 따라서, quinacrine은 H+-ATPase에 의한 수소이온이동 활성측정에 이용된다. 본 연구에서는 토마토 뿌리조직에서 분리한 마이크로솜에서 quinacrine의 형광성을 이용한 H+-ATPase 활성측정의 최적 조건을 조사하였다. Quinacrine의 형광변화는 반응용액 중의 단백질 함량이 0.43㎍/㎕에서 25-26% 감소하여 10%의 quinacrine 형광을 감소시키는 데 약 100 nmol/min의 H+-ATPase 활성이 필요함을 알 수 있었다. Quinacrine의 최대 형광변화는 pH 7.0-7.2 범위와 2 mM Mg2+ 조건에서 일어났다. 이것은 기존에 보고한 H+-ATPase의 특성과 잘 일치하여, quinacrine의 형광변화가 H+-ATPase의 활성을 잘 반영하고 있음을 보인다. 원형질막 및 액포막 H+-ATPase들의 선택적 저해제인 vanadate와 NO3-는 각각의 효소에 의한 수소이온이동 활성을 저해하는데 성공적임을 확인하였다. 이상의 결과로 quinacrine이 토마토 뿌리조직에서 분리한 마이크로솜의 수소이온이동 활성측정에 유용하게 이용될 수 있음을 확인하였다. Quinacrine, a pH-sensitive fluorescence probe, which exists either as an unprotonated fluorescence form or protonated nonfluorescence form, can be used to measure the proton transport activity of H+-ATPase. Quinacrine was used to determine the optimal conditions for measuring the activity of microsomal H+-ATPases prepared from the roots of tomato plants. The amount of quinacrine fluorescence quenching obtained at 0.43 ㎍/㎕ of microsomal protein concentration was 25-26%, which shows that the enzyme activity of 100 nmol/min decreases 10% of quinacrine fluorescence. Maximal fluorescence quenching was obtained at pH 7.0-7.2 and 2 mM Mg2+. Because the activity of microsomal H+-ATPase is also maximal at these conditions, the quinacrine fluorescence well represents the activity of H+-ATPase. Vanadate and NO3-, specific inhibitors of plasma and vacuolar H+-ATPase, respectively, were successfully applied to inhibit the quinacrine fluorescence quenching mediated by the corresponding H+-ATPase. These results imply that quinacrine is a useful tool for measuring the proton transport activities of microsomes obtained from the root tissued of tomato plants.

敗醬草가 癌細胞柱에 미치는 影響

申大澈,權貞南,金瑩均,韓宗鉉,김재석,김재섭 동의대학교 한의학연구소 1997 동의한의연구 Vol.1 No.-

The purpose of this study was to investigate effect of water extract of Patriniae Herba on the poliferation of human cancer cell-lines. The effects of Patriniae Herba on the poliferation of A43l, HeLa, MOLT-4, K562 cells, Balb/c 3T3 cells, mouse thymocytes, splenocytes and human lymphocytes were estimated by MTT colorimetric assay, The results were as follows; 1. Patriniae Herbs did not effect A43l, HeLa, MOLT-4, K562 cells. 2. The cytotoxicity of mitomycin C on MOLT-4 cells was increased by the combination of Patriniae Herba. 3. Patriniae Herba inhibited the proliferation of Balb/c 3T3 cells. 4. Patriniae Herba stimulated the proliferation of thymocytes. 5. Patriniae Herba stimulated the proliferation of splenocytes. 6. Patriniae Herba stimulated the proliferation of human lymphocytes.

축산페수처리를 위한 탈수기와 침전조 개발에 관한 연구

신대윤,최현규 조선대학교 환경공해연구소 1998 環境公害硏究 Vol.14 No.-

Centrifugal filter and filtration type sedimentation tank are built to investigate their capacity and the optimal condition to treat swine wastewater physical-mechamically. 1. As the result of the separation and the experiment of sludge which is over 0.2??of the diameter of the particles, in 180rpm of the rotation speed and 50 ??min of filtration velocity of centrifugal filter, 1.3-4.7kg of sludge, 60-70% of hydration rot per 1m^(3) of filtrates, was removed. 2. Removal rate of SS of filtrates through filtration type sedimentation tank was more than 90%, and BOD and COD more than 50% when filtrate had been kept for 6 months in air-tight container, it generated a little gas and little precipitation.

음이온에 의한 토마토 뿌리조직 마이크로솜 H^+-ATPase 활성 저해

신대섭,조광현,김영기 충북대학교 첨단원예기술개발연구센터 2000 연구보고서 Vol.5 No.-

식물 뿌리세포의원형질막 및 액포막에 위치하는 H+-ATPase들은 세포의 여러 가지 생리활성에 중요한 역할을 수행한다. H+-ATPase의 생리활성 특성을 조사하기 위하여 토마토 뿌리조직으로부터 마이크로솜을 분리하고, H+-ATPase 활성에 미치는 음이온의 효과를 조사하였다. 다양한 종류의 음이온들이 H+-ATPase의 활성을 저해함을 확인하였으며, 이들 중 특히 효소의 저해정도가 다른 citrate와 인산을 선택하여 작용특성을 조사하였다. Citrate에 의한 ATPase 활성저해는 3mM 이상에서 나타났고, 20 mM citrate는 활성을 50-60% 저해하였다. 그러나, citrate,의 저해효과는 MG2+의 농도를 증가시킬수록 감소하여, citrate에 의해 저해된 ATPase 활성은 Mg2+에 의해 회복되는 것으로 나타났다. 즉, 7 mM Mg2+을 첨가하였을 때, citrate에 의한 활성저해는 관측되지 않았고 ATPase 활성은 대조활성과 비슷한 수준으로 회복하였다. 이러한 결과는 citrate가 Mg2+을 chelation함으로써 H+-ATPase의 활성을 저해하기 때문이다. 한편, 인산에 의한 ATPase 활성저해는 3mM 이상의 농도에서 나타났고, 30 mM 인산은 ATPase의 활성을 50% 저해하였다. 인산에 의해서 저해된 ATPase의 활성은 MG2+의 농도증가에 의해 회복되지 않아, 인산에 의한 저해효과는 Mg2+과 무관하였다. H+-ATPases located on plasma and vacuolar membranes play major roles in various cellular physiological processes. In order to investigate the physiological roles of H+-ATPases, microsomes were prepared from tomato roots and the effects of various anions were measured on the activities of H+-ATPases. H+-ATPase was inhibited by various anions. Citrate and phosphate were chosen to investigate detailed inhibitory mechanisms on ATPases since they showed different levels of inhibition. Inhibitory effect of citrate was observed at the concentrations above 3 mM. when 20 mM citrate was added, the H+-ATPase activity was decreased by 50-60%. However, the inhibitory effect of citrate was decreased by increasing the concentration of Mg2+. The citrate-induced inhibited activity was recovered by the addition of Mg2+. Addition of 7 mM Mg2+ completely removed the inhibitory effect of citrate and the activity recovered to the level of the control experiment. These results imply that citrate chelates Mg2+ and thus inhibits H+-ATPases. Meanwhile, the inhibitory effect of phosphate was observed at the concentration above 3 mM and the activity was decreased by 50% in the presence of 30 mM phosphate. Further addition of Mg2+ showed no recovery on the activity. These results imply that the inhibitory effect of phosphate is not dependent upon the concentration of Mg2+. Key words: anion, citrate, phosphate, H+-ATPase, tomato roots Abbreviation: PK, pyruvate kinase; LDH, lactate dehydrogenase; HEPES, N-(2-hydroxyethyl)-piperazine-N'-(2-ethanesulfonic acid); NADH, nicotinamide adenine dinucleotide

일과성 전 기억상실증으로 나타난 크로이츠펠트 : 야콥병 1례

신대수,이승희,이한샘,박성욱,박현영,장혁,장성조,김요식,조광호 圓光大學校 醫科學硏究所 2004 圓光醫科學 Vol.19 No.1

加味薏苡仁湯의 消炎效果 및 成分에 關한 硏究

申鉉大,金性洙 慶熙大學校 1985 論文集 Vol.14 No.-

Kamieuiyeeintang is a combined herb medicine which is used to treat inflammatory illness in oriental medicine field. In order to make clear the anti-inflammatory effects on rats and to find the effective constituents of this herb medicine, author extracted the herbs with water and methanol.. The water extracted portion was administered through mouth to rats and the methanol extraction were used as the samples in HPLC injection to find out the anti-inflammatory constituent by comparing the standard oleanolic acid. The following results were obtained. 1. The extract of Kamieuiyeeintang (100 ㎎/㎏) showed high inhibitory percent of 35.40-47.12 on rat. The aspirin (400 ㎎/㎏) which was used as a control showed 10.42-16.68 of the inhibitory percent. 2. Among the nine herbs of this Kamieuiyeeintang Rheum coreanum Nakai showed the highest swelling percent of 80.60 on the rat hind paw and Taraxacum officinale Wigg was the lowest value of 21.85%. 3. On the HPLC chromatogram Rheum Coreanum Nakai has largest number of peaks and Trichosanthes kirilowii Regel had only 1 peak. 4. In comparing with the standard oleanoloic acid, Paterinia scabiosifolia Fischer showed same peak pattern and retention time with the oleanolic acid. 5. It did not make any relationship between of peak on the chromatogram and anti-inflammatory effect. According to the above results, it is concluded that Kamieuiyeeintang is recognized as a therapeutics for inflammatory diseases and contains the same constituent as oleanolic acid which is a standard therapeutics of inflammation.

筋骨格系 領域에서 Thermography의 臨床的 活用에 對한 考察

신현택,정석희,이종수,김성수,신현대 경희대학교 동서의학연구소 2001 東西醫學硏究所 論文集 Vol.2000 No.-

근골격계 질환은 임상에서 가장 흔하게 접할 수 있는 대상의 하나로 그 진단과 평가가 매우 중요하며 여러 가지 진단기기와 평가방법이 이용되고 있다. Thermography는 기존의 진단기기와 달리 질병의 기능적인 측면을 평가할 수 있다는 점과 통증과 같은 주관적인 표현을 어느정도 객관화할 수 있다는 점에서 임상적 가치가 고려되고 있다. 본 연구는 최근 국내외의 임상 논문을 대상으로 근골격계 영역에서 Thermography의 활용 동향을 고찰하여 진단적 가치를 확인하고자 하였다. Thermography는 척추신경병증, 근막동통증후군, 말초신경병증, 반사성 교감신경 이영양증, 레이노증후군, 악관절 장애 및 일부 기타 질환에서 적용되었으며, 대부분의 질환에서 보조적인 진단기기로 활용가치가 있었다. 특히, 질병의 경과 관찰과 약물 및 기타 처치에 대한 효과 판정에 유용하였으며, 레이노 증후군 및 반사성 교감신경 이영양증과 같은 질병에서는 일차적인 진단기기로도 활용이 가능하였다. 향후 근골격계 영역에서 Thermography의 보다 폭넓은 적용을 위해서는 질환에 따른 적절한 촬영방법의 개발 및 결과 판독에 대한 객관성 확보가 있어야 할 것이다. The purpose of this study is to assess the usefulness of thermography in patients with musculoskeletal disease. Thermography is noninvasive, easy to reading, objective and physiologic instrument by measuring and imaging infrared energy emitted from skin surface. Thermography can show the skin temperature changes in various conditions of the body with musculoskeletal disease. This literature review was done for the usefulness of thermography in diagnosing musculoskeletal disease. In conclusion, thermography was adapted in radiculopathy, MPS, peripheral neuropathy, RSD, Raynaud's phenomen, TMJ dysfunction, etc. It was useful as a secondary diagnostic method in those diseases, also possible as a primary diagnostic method in RSD, Raynaud's phenomen. And, it might be reliable tool for estimating disease procedure and consequence after treatment. But, the objectivity of the reading and the development of the operating method are required for further adaptation in musculoskeletal disease.