방아의 각종 용매 추출물의 항산화 효과

원선임,지옥화,양차범 漢陽大學校 韓國生活科學硏究所 1995 韓國 生活 科學 硏究 Vol.- No.13

Bangah(Agastache rugosa O. Kuntze), one of the herbs grown in Korea, was investigated for its antioxidant activity. Several antioxidative fractions was obtained from Bangah powder by extraction with variours single and mixed solvents. The free, esterified and insoluble-bound phenolic acids were fractioned with using methanol - acetone solvents and added into the soybean oil and lard at the level of 200ppm. The antioxidative activity was measured by peroxide value, carbonyl value and induction time during storage at 60℃. The results were as follows : 1.The extract yield obtained from Bangah powder was increased as the polarity of the solvents increased. 2.The antioxidative effect of the Bangah extracts obtained with single solvent extraction was found in lard in the order of dioxane>methanol>ethyl ether, while those extracts showed little effects on soybean oil. Extraction with using mixed solvents showed no significant improvement in the antioxidative effects. 3.A significant antioxidative effect of phenolic acid fraction was observed on lard, while the effecet was little on soybean oil. The insoluble phenolic arid fraction among the three phenolic acid fractions showed the highest antioxidative effect. When the phenolic acid fractions were added in the range of 200∼1000ppm, the antioxidant effect was observed at 1000ppm for soybean oil and 400ppm for lard.

Resistance of uterine radial artery blood flow is positively correlated with peripheral blood NK cell fraction in patients with unexplained recurrent spontaneous abortion

( Hwa Seon Koo ),( Hyun Kyung Ahn ),( Chan Woo Park ),( Ji Hee Yoo ),( Sun Hwa Cha ),( Inn Soo Kang ),( Kwang Moon Yang ) 대한산부인과학회 2011 대한산부인과학회 학술대회 Vol.97 No.-

The likelihood of achieving pregnancy through timed coitus in young infertile women with decreased ovarian reserve

Koo, Hwa Seon,Song, In Ok,Cha, Sun Hwa,Park, Chan Woo,Kim, Hye Ok The Korean Society for Reproductive Medicine 2018 Clinical and Experimental Reproductive Medicine Vol.45 No.1

Objective: To evaluate the pregnancy rate and time to pregnancy after timed coitus with or without superovulation in infertile young women younger than 35 years old with low serum $anti-M{\ddot{u}}llerian$ hormone (AMH) levels ( < 25th percentile). Methods: A total of 202 patients younger than 35 years old were recruited retrospectively between 2010 and 2012. Ninety-eight women had normal serum AMH levels (25-75th percentile), 75 women had low serum AMH levels (5th ${\leq}$ & < 25th percentile) and 29 women had very low serum AMH levels ( < 5th percentile), according to reference values for their age group. Results: The clinical pregnancy rate was positively associated with AMH levels, but this trend did not reach statistical significance (43.9% vs. 41.3% vs. 27.6% in the normal, low, and very low AMH groups, respectively). The time to pregnancy was longer in the very low AMH group than in the normal AMH group ($13.1{\pm}10.9months$ vs. $6.9{\pm}6.1months$, p= 0.030). The cumulative live birth rate over 18 months was lower in the very low AMH group than in the normal AMH group, with marginal significance (20.0% vs. 55.9%, p= 0.051). The duration of infertility was negatively correlated with achieving pregnancy (odds ratio, 0.953; 95% confidence interval, 0.914-0.994; p= 0.026). Conclusion: Conservative management, such as timed coitus with or without superovulation, should be considered in young patients who have low ovarian reserve without any infertility factors. However, for women with a long duration of infertility or very low serum AMH levels, active infertility treatment should be considered.

Effect of Different Strengths Using Hoagland’s Nutrient Solution on The Growth and Intermal Quality Change of ‘Pakchoi’ Using MK System

차선화(Seon-Hwa Cha),정성우(Sung-Woo Jeong),허무룡(Moo-Ryong Huh),박중춘(Joong-Choon Park) 한국원예학회 2009 한국원예학회 학술발표요지 Vol.2009 No.5

Laparoscopic management of early primary peritoneal pregnancy: a case report

Koo, Hwa-Seon,Bae, Ju-Youn,Kang, Inn-Soo,Koong, Mi-Kyoung,Kim, Hye-Ok,Cha, Sun-Hwa,Choi, Min-Hye,Kim, Ji-Young,Yang, Kwang-Moon The Korean Society for Reproductive Medicine 2011 Clinical and Experimental Reproductive Medicine Vol.38 No.2

Peritoneal pregnancy is an implantation in the peritoneal cavity exclusive of tubal, ovarian, or intra-ligamentary implantations. This is a rare obstetric complication with high maternal mortality and even higher perinatal mortality, and secondary type was most common. Risk factors for peritoneal pregnancy are previous history of extrauterine pregnancy or tubal surgery pelvic post-inflammatory status or presence of an intrauterine device. As it is a life-threatening condition, expectant management carries a risk of sudden life-threatening intra-abdominal bleeding and a generally poor fetal prognosis. So, when it is recognized, immediate termination of pregnancy is usually recommended. Early diagnosis of peritoneal pregnancy is difficult, but is important by their life threatening progress course to patients. Recently, we experienced primary peritoneal pregnancy which meets both the original and modified criteria. In this paper, we reported the case of early diagnosed and successfully treated peritoneal pregnancy despite of their diagnosis was incidentally.

A high response to controlled ovarian stimulation induces premature luteinization with a negative impact on pregnancy outcomes in a gonadotropin-releasing hormone antagonist cycle

Koo, Hwa Seon,Cha, Sun Hwa,Kim, Hye Ok,Song, In Ok,Min, Eung Gi,Yang, Kwang Moon,Park, Chan Woo The Korean Society for Reproductive Medicine 2015 Clinical and Experimental Reproductive Medicine Vol.42 No.4

Objective: The goal of this study was to investigate the relationship between serum progesterone (P4) levels on the day of human chorionic gonadotropin (hCG) administration and the pregnancy rate among women undergoing controlled ovarian stimulation for in vitro fertilization (IVF) or intracytoplasmic sperm injection-embryo transfer (ICSI-ET) using a flexible antagonist protocol. Methods: This prospective study included 200 IVF and ICSI-ET cycles in which a flexible antagonist protocol was used. The patients were divided into five distinct groups according to their serum P4 levels at the time of hCG administration (0.80, 0.85, 0.90, 0.95, and 1.00 ng/mL). The clinical pregnancy rate (CPR) was calculated for each P4 interval. Statistically significant differences were observed at a serum P4 level of 0.9 ng/mL. These data suggest that a serum P4 concentration of 0.9 ng/mL may represent the optimal threshold level for defining premature luteinization (PL) based on the presence of a significant negative impact on the CPR. Results: The CPR for each round of ET was significantly lower in the PL group defined using this threshold (25.8% vs. 41.8%; p=0.019), and the number of oocytes retrieved was significantly higher than in the non-PL group ($17.3{\pm}7.2$ vs. $11.0{\pm}7.2$; p=0.001). Elevated serum P4 levels on the day of hCG administration were associated with a reduced CPR, despite the retrieval of many oocytes. Conclusion: Measuring serum P4 values at the time of hCG administration is necessary in order to determine the optimal strategy for embryo transfer.

Comparison of Volatile Flavor Compounds in Commercial Black Onion Extracts

Seon-Young Jeon(전선영),Eun-Jeong Jeong(정은정),Jeong-Hwa Baek(백정화),Yong-Jun Cha(차용준) 한국생명과학회 2011 생명과학회지 Vol.21 No.12

현재 시중에 판매되고 있는 흑양파추출액 3종류(창녕, 무안, 증평 지역산 제품)를 구입하여 관능적 요소 중 가장 중요한 휘발성 향기성분을 분석하여 제품의 품질에 대한 기초자료로 활용하고자 하였다. 시판 흑양파추출액의 휘발성 향기성분을 분석한 결과, 총 51종으로 카르보닐화합물류 15종, 함황화합물류 8종, 방향족화합물류 6종, 퓨란류 6종, 질소화합물류 3종, 알콜류 2종, 산류 2종 및 기타 10종이 검출되었다. 카르보닐화합물 중 2-methylbutanal, 3-methylbutanal, nonanal 및 benzaldehyde가 모든 시료에서 검출되었다. 함황화합물류에서는 dimethyl disulfide와 dimethyl trisulfide가 가장 높은 함량으로 검출되었으며 흑양파추출액의 주요 향기성분에 관여할 것으로 사료되었다. 항산화성을 가지는 thiophene 유도체와 항암효과를 지닌 methylpropyl disulfide, methyl-(Z)-propenyl disulfide 및 methyl-(E)-propenyl disulfide 등은 2 제품(A, B)에서만 검출되었다. 비효소적갈변유도물질인 furfural이 모든 시료에서 높은 함량으로 나타났으며 pyrazine류 및 acetic acid 도 많은 함량으로 검출되어 이들 향기성분들이 시판 흑양파추출액의 휘발성 성분에 특징적인 냄새성분으로 기여할 것으로 사료되었다. Volatile flavor compounds of 3 commercial products of black onion extract (produced in Changnyeong, Muan and Jeungpyeong) purchased in the online/offline markets were analyzed. A total of 51 compounds were detected in samples by solid phase microextraction (SPME)/GC/MSD, consisting mainly of carbonyl compounds (15), sulfur-containing compounds (8), aromatic compounds (6), furans (6), nitrogen-containing compounds (3), alcohols (2), acids (2) and miscellaneous compounds (10). Among carbonyls, 4 compounds including 2-methylbutanal, 3-methylbutanal, nonanal and benzaldehyde were detected in all samples, while two sulfur containing compounds, dimethyl disulfide and dimethyl trisulfide, were detected in high amounts and considered to be key flavors in black onion extracts. Particularly, thiophenes and 3 sulfur containing compounds (methylpropyl disulfide, methyl-(Z)-propenyl disulfide and methyl-(E)-propenyl disulfide) were detected only in two products. With acetic acid, furfural and pyrazines that formed through Maillard reaction during black onion aging were detected in high amounts in all samples. This also contributed to characteristic flavors such as roasted, sweet, and sour, in the flavor of black onion extracts.

Potential of Onion Peel Extract as a Functional Ingredient for Functional Foods

Seon-Young Jeon(전선영),Jeong-Hwa Baek(백정화),Eun-Jeong Jeong(정은정),Yong-Jun Cha(차용준) 한국생명과학회 2012 생명과학회지 Vol.22 No.9

본 연구에서는 양파가공부산물인 양파껍질을 이용하여 기능성 식품소재로 개발하고자 양파껍질추출물을 제조하였으며 양파껍질추출물에 대한 생리적 기능성과 같은 품질 특성을 조사하였다. 양파껍질추출물에서는 K가 13,767.56~15,506.78 ppm으로 가장 많은 함량을 차지하였고 Na이 8,602.44~9,796.00 ppm, Ca은 4,255.78~4,903.33 ppm으로 세 번째로 많은 함량을 차지하였다. 그리고 Mg, P, Fe은 각각 1,433.11~1,561.22 ppm, 1,212.44~1,428.89 ppm, 760.67~858.89 ppm의 함량을 차지하였고 Zn은 34.11~66.89 ppm으로 미량 검출되었다. Total phenol 함량은 598.57~626.73 mg/g, total flavonoid 함량은 211.73~238.52 mg/g 범위로 quercetin 함량은 93.67~107.29 mg/g의 범위로 나타났다. 양파껍질추출물의 DPPH radical 소거활성은 100 ppm에서 16.45~17.92%, 200 ppm은 29.96~35.41%, 1,000 ppm은 81.05~84.60%의 소거활성을 보였고, SOD 유사활성은 10,000 ppm에서 31.92~39.29%, 20,000 ppm에서 85.85~91.58%의 활성을 나타내었다. 혈전용해능은 20,000 ppm 농도에서는 plasmin에 비해 약 3배 높은 활성을 가졌다. 따라서 양파껍질 추출물은 항산화력 및 항혈전효과능을 가진 기능성소재로서의 활용 가능성을 보였다. Onion peels is a natural source of high-value functional ingredients produced in the onion industry without suitable processing. The objective of this study was to evaluate characteristics of onion peel extract (OPE), including its biological activities, obtained from solvent extraction in 3 times pilot scales (Lot A, B, and C). Mineral analysis showed that K was present in the largest amount (13,767.56-15,506.78 ppm), followed by Na and Ca at 8,602.44-9,796.00 ppm and 4,255.78-4,903.33 ppm, respectively. The amounts of total phenol, total flavonoid, and quercetin in the OPE were in the ranges of 598.57~626.73, 211.73~233.64, and 93.78~107.29 mg/g, respectively. The biological activities such as antioxidant and effects of fibrinolysis increased in parallel with the concentration of OPE. The IC50 value of DPPH radical scavenging activity was in the range of 517.58~557.32 ppm in the OPE. The IC50 value for superoxide dismutase (SOD)-like activity was in the range of 11,900.91~12,690.35 ppm. A clear zone of OPE (20,000 ppm) in fibrinolysis test was three times higher than the plasmin as a reference. In conclusion, OPE could be used as a good source of antioxidants and fibrinolytic activities.

Role of Soluble VEGF Receptor (sfltl) in Diabetic Nephropathy

차대룡 ( Cha Dae Lyong ),지이화 ( Ji I Hwa ),홍동기 ( Hong Dong Gi ),성수아 ( Seong Su A ),한금현 ( Han Geum Hyeon ),강영선 ( Kang Yeong Seon ),한상엽 ( Han Sang Yeob ),조상경 ( Jo Sang Gyeong ),조원용 ( Jo Won Yong ),김형규 ( Kim 대한신장학회 2003 춘계학술대회 초록집 Vol.22 No.1

건강기능식품 기능성원료로서 창녕양파추출액의 지표성분 Quercetin 분석법

전선영(Seon-Young Jeon),정은정(Eun-Jeong Jeong),백정화(Jeong-Hwa Baek),차용준(Yong-Jun Cha) 한국식품영양과학회 2011 한국식품영양과학회지 Vol.40 No.4

개별인정형 건강기능식품 기능성 원료로 개발한 창녕양파추출액의 표준화를 위해 지표성분으로 quercetin을 설정하였으며, HPLC를 이용하여 지표성분 quercetin의 분석법을 확립하며 그에 따른 유효성 검정을 실시하고자 하였다. 유효성 검정 결과, 본 시험법에서 표준용액의 피크유지시간과 창녕양파추출액의 피크유지시간이 일치하였으며 동일한 spectrum을 나타내어 특이성을 확인하였다. 검량선의 상관계수(R2)는 0.9986로 높은 직선성을 보여 분석에 적합함을 알 수 있었으며, 검출한계는 0.2 mg/L, 정량한계는 0.5 mg/L로 설정되었다. Quercetin의 회수율은 0.05 mg/mL에서는 82.36~95.26%, 0.075 mg/mL는 82.70~98.24%, 0.1 mg/mL은 87.91~95.11%의 범위의 회수율을 보였으며, intra-day에서의 정밀도(RSD)는 0.10~3.28%, inter-day에서는 0.96~5.79%의 정밀도를 나타내어 창녕양파추출액의 지표성분인 quercetin의 분석법은 적합한 시험법임이 검증되었다. Validation of quercetin as a marker compound in the standardization of Changnyeong onion extract developed for functional health food was attempted by analytical method. The specificity was satisfied with retention time and photo diode array (PDA) spectrum by analysis of quercetin using HPLC and comparison with standard compound. It showed a high linearity in the calibration curve as coefficient of correlation (R²) of 0.9986, and the limit of detection (LOD) and limit of quantitation (LOQ) were 0.2 mg/L and 0.5 mg/L, respectively. Recovery rate test with quercetin concentration of 0.05, 0.075 and 0.1 mg/mL was revealed in the high range of 82.36~95.26%, 82.70~98.24% and 87.91~95.11%, respectively. The intra-day and inter-day precision in quercetin for Changnyeong onion extracts was 0.10~3.28% and 0.96~5.79%, respectively. Therefore, application of quercetin was validated in analytical method as a marker compound in Changnyeong onion extracts.