http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Sang-Yoon Ha (검색결과 2,396 건)
- 무료
- 기관 내 무료
- 유료
-
Chun, Yoon-Keun,Ha, Joo-hun,Hong-Jung-Woo,Oh, Soo-Myung,Kim, Sung-Soo 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1
Yoon-Keun Chun¹,Joohun Ha□Hong-Jung Woo□, Soo Myung Oh□,Sung Soo Kim□ ¹Department of Molecular Biology, College of Medicine,²Department of Surgery, college of Medicine,³Department of Internal Medicine, College of Oriental Medicine,and ⁴East-Weat Medical Reserch Institute,Kyung Hee University, Seoul, Korea. The HBV DNA Amounts in Serum Have No relationship with ALT level and Hetergeneous Population Coexits in Chronic Hepatitis B Virus Infection. Proceedings of International Symposium on East-West Medicine, Seoul. 212-230, 1999. -Hepatitis B is caused by hepadnavirus. Hepatitis B virus replicates through 3.5kb pregenomic RNA intermediate which is regulated by core promoter. Pathogenesis of hepatitis B virus has been bilieved the result of host immune response. But recently many studies have reported that high level of viral replication caused by mutation in core promoter might result in severs hepatitis. But these studies were performed in vitro, not in vivo. So there is yet debate about which factor, viral of host factor, is more important in pathogenesis of hepatitis B virus. So we measured real viral replication level in 204 chronic hepatitis B patients by quantifying HBV DNA from sera by our novel PCR-based more sensitive method, and compared these results with ALT level measured from same sera, which indicates liver cell damage. Surprisingly there are no significant correlation between HBV DNA quantity and ALT level. Then we cloned core promoter region. In SSCP, we found that many viral mutants coexist in one patient. Base on SSCP result, we chose main viral core promoter type in each patients, which is thought to determine overall viral replication level in this patient. Main type of core promoter region of each 41 patients were directly sequenced. And with these we measured promoter activity by luciferase assay system and compared promoter activity with on another. We found tha there were some differences in promoter activity according to core promoter sequences. And we constructed replication-competent viral constructs with core promoter from 41 patients and Transfected these into HepG2 cell and measured HBV DNA by southern blot. There were also differences in HBV DNA quantity according to core promoter sequences. On these all results we investigated correlation between the effect of HBV core promoter on viral replication in vitro and HBN DNA quantity, ALT level from sera of each patients. We found there is no significant correlation among them. As a result, we concluded that in determining severity chronic hepatitis B patients, host factors of each patient is more important rather than replicative activity of virus itself.
-
논문 : 청원-충주지역 수막재배용 지하수 사용량 및 배출량 분석
문상호 ( Sang Ho Moon ),하규철 ( Kyoo Chul Ha ),김용철 ( Yong Cheol Kim ),윤필선 ( Pil Sun Yoon ) 대한지질공학회 2012 지질공학 Vol.22 No.4
국내 수막재배지는 시설 증대로 인한 지하수 취수량 감소의 어려움을 겪고 있다. 이 연구는 이들 지역 중 청원-충주지역을 대상으로 지하수 이용량 및 배출량을 측정하고, 이를 토대로 국내 수막재배지에서의 지하수 이용량을 추정하였다. 대표 관정들의 모터사양과 1일 지하수 토출 능력과의 관계, 1일 최저 기온0℃ 이하의 기록 일수, 최저 기온 변화에 따른 모터 가동 상황 등을 토대로 하여, 청원지역에서 2011~2012년 겨울철 수막재배에 이용된 지하수 이용량을 추정하면 1ha 당 53,138m3이 된다. 이에 의한 면적 대비 국내 수막재배지 (10,746ha)의 지하수 이용 총량은 약 5.7억 m3이며, 이는 국내 농업용수 지하수 이용량 16.9억 m3 33.7%에 해당된다. 2012년 2월 9일부터 22일까지 면적 4ha의 청원지역 배수로에서 측정한 1일 지하수 배출량은 2,079~2,628 m3범위(평균 2,341 m3)로서, 수막재배 면적 1ha 당 1일 평균 지하수 배출량이 585m3인 것으로 나타났다. 수막재배 일수 94일을 적용하면, 청원지역에서 2011년~2012년 겨울철에 수막재배에 이용된 지하수 이용량은 54,990m3/ha이다. 이에 의한 면적 대비 국내 수막재배지의 지하수 이용 총량은 약 5.9억 m3이며, 이는 전체 농업용수 지하수 이용량의 약 34.9%에 해당된다. 충주지역에서는 수막재배지 1ha 당 1일 지하수 배출량이 805 m3 미만일 것으로 추정되었다. 이 지역에서의 2011년~2012년 겨울철 수막재배 일수 108일을 적용하면, 면적 대비 국내 수막재배지의 지하수 이용량은 전체 농업용수 지하수 이용량의 55% 미만일 것으로 추정된다. Korean agricultural areas that employ water curtain cultivation (WCC) have recently suffered extensive groundwater short-ages due to an increase in the number of facilities. The primary focus of this study is to measure the daily groundwater use and discharge rates in the Cheongweon and Chungju pilot areas, while the second focus is to estimate the total amount of groundwater used in WCC areas nationwide in Korea. Taking inwo consideration several factors, including motor type, out-flow abilities of wells, records of daily minimum temperatures below 0℃, and the number of running wells accrding to weather variations, we estimated that 53,138m3/ha of groundwater had been used in the 4-hectare Cheongweon pilt area during the winter period of late 2011 through early 2012. On a prorated areal basis, we can calculate that the total ground-water used nationwide was 0.57 billion m3 in WCC areas of 10,746m2. This value is equivalent to 33.7% of the total agri-cultural groundwater use (1.69 billion m3) in Korea. During 9-22 February 2012, the daily water discharge rate in the 4-ha Cheongweon pilot area raged from 2,079 to 2,628m3, averaging 2,341m3. Combining this value with meteorological records for 94 days with a daily minimum temperature below 0℃ results in an estimated groundwater volume of 54,990m3/ha for the pilot area during the 2011-2012 winter period. The total amount of groundwater used nationwide in WCC areas would then be 0.59 billion m3, equivalent to 34.9% of the total agricultural groundwater use in Korea. In the Chungju area, the groundwater discharge rate was estimated to be less than 805m3/ha. This value, combined with weather data for 108 days with a daily minimum temperature below 0℃ in this area, can be applied to infer that the total groundwater vol-ume used in WCC areas nationwide is no more than 55% of the total agricultural groundwater use in Korea.
-
수용액중의 수은전극에서 바나듐-디에틸렌트리아민 펜타아세트산염의 환원 및 평형연구
정기석,손세철,하영경,엄태윤,윤석승,Ki-Suk Jung,Se Chul Sohn,Young Kyung Ha,Tae Yoon Eom,Sock Sung Yun 대한화학회 1989 대한화학회지 Vol.33 No.1
0.5M $NaClO_4$ 수용액중의 수은전극에서 바나듐-디에틸렌트리아민펜타아세트산(DTPA)염의 전기화학적 환원 및 평형을 온도 25$^{\circ}C$와 3.2 < pH < 10.5 에 걸쳐서 연구하였다. 바나듐(III)-DTPA착물은 모든 pH에 걸쳐서 V${\cdot}A^{3-}$-(A=DTPA)로서 존재하고 EDTA같은 다른 디아민카르복시산과의 착물들과 달리 수소첨가가 일어나지 않고 $OH^-$도 배위되지 않으며 가역적으로 바나듐(II)-DTPA착물로 환원된다. 3.2 < pH < 5.9에서는 전극반응이 $V{\cdot}A^{2-}+H^++e^-=V{\cdot}HA^{2-}$와 같이 진행하며 $V{\cdot}HA^{3-}$ 의 안정도상수는 $3.09{\times}10^{14}$과 같이 구하여졌다. $VO^{2+}$이온의 경우 pH적정결과 착화반응은 $VO^{2+}+H_2A^{3-}=VO{\cdot}HA^{2+}H^{+}$ 및 $VO{\cdot}HA^{2-}=VO{\cdot}A^{3+}+H$ 와 같이 2단계에 걸쳐서 진행되며, $VO{\cdot}HA{2-}$의 산해리상수는 pKa=7.15이다. $VO{\cdot}HA^{2-}$와 $VO{\cdot}A^{3-}$ 의 안정도상수는 각각 $1.41{\times}10^{14}$ 및 $3.80{\times}10^{17}$과 같이 구하여졌다. 바나듐(IV)-DTPA착물은 비가역적으로 바나듐(III)-DTPA착물로 환원되며 이때 전이상수 ${\alpha}$=0.43이다. 더 큰 음의 과전압에서는 2단계에 걸쳐서 환원된다. 이 때 첫째 단계의 환원은 3.2 < pH < 10.5에서 $VO{\cdot}A^{3-}+e{\to}VO{\cdot}A^4$인 것으로 판단되었다. 두번째 단계의 환원은 V(III)의 환원과 같다. $VO{\cdot}HA^{2-}$ 와 $VO{\cdot}A^{3-}$의 확산계수로서 각각 $(9.0{\pm}0.4){\times}10^{-6}cm^2/s$ 및 $(5.9{\pm}0.4){\times}10^{-6}cm^2/s$ 을 구하였다. Reduction and equilibrium of vanadium-DTPA (DTPA = diethylenetriaminepentaacetic acid, $H_5A$) complexes at mercury electrodes are studied in 0.5M $NaClO_4$ aqueous solution at 3.2 < pH < 10.5 and 25$^{\circ}$C. At 3.2 < pH < 5.9, the reduction reaction is $V{\cdot}A^{2-}+H^-+e^-=V{\cdot}HA^{2-}$, while at 5.9 < pH < 10.5 it is $V{\cdot}A^{2-}+H^-+e^-=V{\cdot}A^{3-}$. The stability constants of $V{\cdot}HA^{2-}$ and $V{\cdot}A^{3-}$ are found to be $6.46{\times}10^{9}$ and $3.09{\times}10^{14}$, respectively. V(IV)-DTPA undergoes stepwise complexation as $VO^{2+}+H_2A^{3-}=VO{\cdot}HA^{2+}H^{+}$ and $VO{\cdot}HA^{2-}=VO{\cdot}A^{3+}+H$, where acidity constant of $VO{\cdot}HA^{2-}$- is pKa = 7.15. Stability constants of $VO{\cdot}HA^{2-}$ and $VO{\cdot}A^{3-}$ are found to be $1.41{\times}10^{14}$ and $3.80{\times}10^{17}$, respectively. It is detected that $VO^{2+}-DATA$ is reduced irreversibly to $VO^{2-}$ with the transfer coefficient of $\alpha$ = 0.43. At more cathodic overpotential, the reduction is stepwise as V(IV)${\to}$V(III)${\to}$V(II). The first one corresponds to $VO{\cdot}HA^{2-}+e^{-}{\to}VO{\cdot}HA{3+}$ at 3.2 < pH < 7.2 and $VO{\cdot}A^{3-}+e^{-}{\to}VO{\cdot}A^{4-}$ at 7.2 < pH < 10.5. The second is identical to that of V(III). Diffusion coefficients of $VO{\cdot}HA^{2-}$ and $VO{\cdot}A^{3-}$ are found to be $(9.0{\pm}0.3){\times}10^{-6}cm^2/s$ and $(5.9{\pm}0.4){\times}10^{-6}cm^2/ses$, respectively.
-
-
4.2 PW, 20 fs Ti:sapphire laser at 0.1 Hz
Sung, Jae Hee,Lee, Hwang Woon,Yoo, Je Yoon,Yoon, Jin Woo,Lee, Chang Won,Yang, Jeong Moon,Son, Yeon Joo,Jang, Yong Ha,Lee, Seong Ku,Nam, Chang Hee OPTICAL SOCIETY OF AMERICA 2017 Optics letters Vol.42 No.11
<P>We demonstrated the generation of 4.2 PW laser pulses at 0.1 Hz from a chirped-pulse amplification Ti:sapphire laser. The cross-polarized wave generation and the optical parametric chirped-pulse amplification stages were installed for the prevention of the gain narrowing and for the compensation of the spectral narrowing in the amplifiers, obtaining the spectral width of amplified laser pulses of 84 nm (FWHM), and enhancing the temporal contrast. The amplified laser pulses of 112 J after the final booster amplifier were compressed to the pulses with 83 J at 19.4 fs with a shot-to-shot energy stability of 1.5% (RMS). This 4.2 PW laser will be a workhorse for exploring high field science. (C) 2017 Optical Society of America</P>
-
Effects of Silicon on Ethylene and Salicylic Acid Contents of Rice after Wounding Stress
Yoon-Ha Kim,Muhammad Hamayun,Abdul Latif Khan,Sang-Mo Kang,Joon-Hee Lee,In-Jung Lee 한국작물학회 2010 한국작물학회 학술발표대회 논문집 Vol.2010 No.04
We carried out the effect of Si levels (0.5, 1.0 and 2.0 mM) on ethylene and salicylic acid (SA) of hydroponically grown rice plants, after 6h, 12h and 24h of Si application. All samples were analyzed after applying wound stress for 30 minutes. In 6 h treatments, 0.5 and 1.0 mM of Si decreased ethylene production by 7% and 9.9% respectively, while elevated level of Si (2 mM) insignificantly affected ethylene production under same conditions. However 0.5 and 1 mM of Si enhanced endogenous SA contents by 2.6% and 17.3% respectively, while 2 mM of Si decreased SA contents by 3% as compared to control. Samples analyzed after 12h of 0.5, 1 mM and 2 mM Si application showed reduction of 11%, 4% and 1.7% respectively, while SA contents increased by 4% and 9.5% in 0.5 and 1 mM Si treatments. Samples analyzed after 24 h of Si treatment showed that 0.5 and 1.0 mM of Si inhibited ethylene production by 12.6% and 18.2% respectively, while 2 mM Si increased ethylene production by 11.9% as compared to control. It was observed that the SA content of rice was almost similar to control in 0.5 mM Si applied treatments, while 1 mM and 2 mM of Si increased SA contents by 6.5% and 6.9% as compared to control. It was concluded that Si alleviates the effect of wounding stress in rice by regulating ethylene and SA production.
-
Yoon, Joo Seok,Park, Sang Kyu,Kim, Young Baek,Maeng, Hack-Young,Rhee, Young Ha 충남대학교 생물공학연구소 1997 생물공학연구지 Vol.5 No.-
The weight average molecular weight of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) synthesized by Alcaligenes sp. SH-69 was altered between 3.2 × 10 exp (5) and 1.1 × 10 exp (6) depending upon various culture conditions. It appeared that culture conditions favorable for the efficient production of copolyesters promoted the formation of higher molecular weight copolyesters. Polydispersity indices of isolated copolyesters were in the range of 1.5 to 2.5.
-
Ha, Ji-Hyang,Shin, Jae-Sun,Yoon, Mi-Kyung,Lee, Min-Sung,He, Fahu,Bae, Kwang-Hee,Yoon, Ho Sup,Lee, Chong-Kil,Park, Sung Goo,Muto, Yutaka,Chi, Seung-Wook American Society for Biochemistry and Molecular Bi 2013 The Journal of biological chemistry Vol.288 No.10
<P>Molecular interactions between the tumor suppressor p53 and the anti-apoptotic Bcl-2 family proteins play an important role in the transcription-independent apoptosis of p53. The p53 transactivation domain (p53TAD) contains two conserved Φ<I>XX</I>ΦΦ motifs (Φ indicates a bulky hydrophobic residue and <I>X</I> is any other residue) referred to as p53TAD1 (residues 15–29) and p53TAD2 (residues 39–57). We previously showed that p53TAD1 can act as a binding motif for anti-apoptotic Bcl-2 family proteins. In this study, we have identified p53TAD2 as a binding motif for anti-apoptotic Bcl-2 family proteins by using NMR spectroscopy, and we calculated the structures of Bcl-X<SUB>L</SUB>/Bcl-2 in complex with the p53TAD2 peptide. NMR chemical shift perturbation data showed that p53TAD2 peptide binds to diverse members of the anti-apoptotic Bcl-2 family independently of p53TAD1, and the binding between p53TAD2 and p53TAD1 to Bcl-X<SUB>L</SUB> is competitive. Refined structural models of the Bcl-X<SUB>L</SUB>·p53TAD2 and Bcl-2·p53TAD2 complexes showed that the binding sites occupied by p53TAD2 in Bcl-X<SUB>L</SUB> and Bcl-2 overlap well with those occupied by pro-apoptotic BH3 peptides. Taken together with the mutagenesis, isothermal titration calorimetry, and paramagnetic relaxation enhancement data, our structural comparisons provided the structural basis of p53TAD2-mediated interaction with the anti-apoptotic proteins, revealing that Bcl-X<SUB>L</SUB>/Bcl-2, MDM2, and cAMP-response element-binding protein-binding protein/p300 share highly similar modes of binding to the dual p53TAD motifs, p53TAD1 and p53TAD2. In conclusion, our results suggest that the dual-site interaction of p53TAD is a highly conserved mechanism underlying target protein binding in the transcription-dependent and transcription-independent apoptotic pathways of p53.</P>
-
-
KISS연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
