The Pharmacological Effects of Benachio-F^® on Rat Gastrointestinal Functions

Poudel, Bijay Kumar,Yu, Jae Young,Kwon, Yong Sam,Park, Hyoung Geun,Son, Miwon,Jun, Joon Ho,Kim, Jeong Ah,Kim, Jong Oh The Korean Society of Applied Pharmacology 2015 Biomolecules & Therapeutics(구 응용약물학회지) Vol.23 No.4

Functional dyspepsia (FD) is a prevalent idiopathic upper gastrointestinal (GI) disorder characterized by diverse symptomatology including epigastric pain or discomfort, postprandial fullness, and early satiety. Although its pathophysiological mechanisms have not yet been fully established, the available studies suggest that the etiology of FD is invariably multifactorial. Benachio-F$^{(R)}$ (BF) is a proprietary liquid formulation of 7 herbal extracts that has been proposed to address this multifactorial etiology using multi-drug phytotherapy. The pharmacological effects of BF, in comparison with those of two other herbal products (Whalmyungsu$^{(R)}$; WM and Iberogast$^{(R)}$; IB) were evaluated in rats. In a laparotomy-induced rat model of delayed GI transit, BF significantly accelerated the delayed gastric emptying caused by morphine, apomorphine, and cisplatin, and also significantly increased mean gastric transit, as compared to the control animals. BF markedly increased gastric accommodation in rats and produced higher gastric volume values than did the control treatment. The effects of BF were generally comparable or superior to those of WM and IB in these models. Furthermore, BF significantly stimulated biliary flow, as compared to the control treatment. These results indicated that BF might have great potential as an effective phytotherapeutic agent capable of reducing GI symptoms and increasing quality of life in FD patients.

PEGylated thermosensitive lipid-coated hollow gold nanoshells for effective combinational chemo-photothermal therapy of pancreatic cancer

Poudel, Bijay Kumar,Gupta, Biki,Ramasamy, Thiruganesh,Thapa, Raj Kumar,Pathak, Shiva,Oh, Kyung Taek,Jeong, Jee-Heon,Choi, Han-Gon,Yong, Chul Soon,Kim, Jong Oh Elsevier 2017 Colloids and surfaces Biointerfaces Vol.160 No.-

<P><B>Abstract</B></P> <P>Pancreatic cancer has extremely poor prognosis with an 85% mortality rate that results from aggressive and asymptomatic growth, high metastatic potential, and rapid development of resistance to already ineffective chemotherapy. In this study, plasmonic hollow gold nanoshells (GNS) coated with PEGylated thermosensitive lipids were prepared as an efficient platform to ratiometrically co-deliver two drugs, bortezomib and gemcitabine (GNS-L/GB), for combinational chemotherapy and photothermal therapy of pancreatic cancer. Bortezomib was loaded within the lipid bilayers, while gemcitabine was loaded into the hydrophilic interior of the porous GNS via an ammonium sulfate-driven pH gradient method. Physicochemical characterizations and biological studies of GNS-L/GB were performed, with the latter using cytotoxicity assays, cellular uptake and apoptosis assays, live/dead assays, and western blot analysis of pancreatic cancer cell lines (MIA PaCa-2 and PANC-1). The nanoshells showed remotely controllable drug release when exposed to near-infrared laser for site-specific delivery. GNS-L/GB showed synergistic cytotoxicity and improved internalization by cancer cells. High-powered near-infrared continuous wave laser (λ=808nm) effectively killed cancer cells via the photothermal effect of GNS-L/GB, irrespective of cell type in a power density-, time-, and GNS dose-dependent manner. These results suggest that this method can provide a novel approach to achieve synergistic combinational chemotherapy and photothermal therapy, even with resistant pancreatic cancer.</P> <P><B>Highlights</B></P> <P> <UL> <LI> PEGylated thermosensitive lipid-coated hollow gold nanoshells (GNS-L) were prepared. </LI> <LI> GNS-L were co-loaded with hydrophilic gemcitabine and hydrophobic bortezomib. </LI> <LI> NIR irradiation induced drug release for remotely controlled site-specific delivery. </LI> <LI> GNS-L increased cellular uptake and apoptosis in pancreatic cancer cells. </LI> <LI> Direct photothermal killing of cancer cells was observed on NIR laser irradiation. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Triticumoside induces apoptosis via caspase-dependent mitochondrial pathway and inhibits migration through downregulation of MMP2/9 in human lung cancer cells

Poudel, Barun,Ki, Hyeon-Hui,Luyen, Bui Thi Thuy,Lee, Young-Mi,Kim, Young-Ho,Kim, Dae-Ki Oxford University Press 2016 Acta biochimica et biophysica Sinica Vol.48 No.2

<P>Non-small cell lung cancer (NSCLC) is the major cancer-related death worldwide with only 14% five-year survival rate. Triticumoside, a phenolic compound present in Triticum aestivum sprout extract, has been recognized to have antiobesity and anti-inflammatory effects. However, the effect of triticumoside on cancer cell proliferation and migration has not been studied. In order to elucidate whether triticumoside exhibits an anticancer effect, cells were incubated with different doses of triticumoside, and apoptosis was assessed by observing cell viability, cellular morphological changes, and annexin-V-fluorescein isothiocyanate/propidium iodide staining. Cell cycle analysis, western blotting, wound healing assay, and quantitative-polymerase chain reaction were also performed. Triticumoside exhibited marked cytotoxicity in the cells in dose-and time-dependent manner. Triticumoside caused morphological changes, including cellular rounding, nuclear condensation, and shrinkage. Likewise, triticumoside enhanced the sub-G1 proportion of cells. Additionally, triticumoside regulated expression of apoptosis-associated proteins, such as B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X, and procaspase-3/9. Triticumoside also inhibited migration of the cells through downregulation of matrix metalloproteinase-2/9 (MMP2/9). Collectively, these results suggest that triticumoside induces apoptosis through caspase-dependent mitochondrial pathway and suppresses migration via inhibition of MMP2/9 in NSCLC A549 cells.</P>

폐실리콘슬러지를 이용한 실리콘 분말의 재활용 방법에 관한 연구

( Poudel Jeeban ),남석희,임대원,최경석,장제일,오세천 한국공업화학회 2011 한국공업화학회 연구논문 초록집 Vol.2011 No.-

Recombinant human IGF-1 produced by transgenic plant cell suspension culture enhances new bone formation in calvarial defects

Poudel, Sher Bahadur,Bhattarai, Govinda,Kook, Sung-Ho,Shin, Yun-Ji,Kwon, Tae-Ho,Lee, Seung-Youp,Lee, Jeong-Chae Elsevier 2017 Growth hormone & IGF research Vol.36 No.-

<P><B>Abstract</B></P> <P>Transgenic plant cell suspension culture systems have been utilized extensively as convenient and efficient expression systems for the production of recombinant human growth factors. We produced insulin-like growth factor-1 using a plant suspension culture system (p-IGF-1) and explored its effect on new bone formation in calvarial defects. We also compared the bone regenerating potential of p-IGF-1 with commercial IGF-1 derived from <I>Escherichia coli</I> (e-IGF-1). Male C57BL/6 mice underwent calvarial defect surgery, and the defects were loaded with absorbable collagen sponge (ACS) only (ACS group) or ACS impregnated with 13μg of p-IGF-1 (p-IGF-1 group) or e-IGF-1 (e-IGF-1 group). The sham group did not receive any treatment with ACS or IGFs after surgery. Live μCT and histological analyses showed critical-sized bone defects in the sham group, whereas greater bone formation was observed in the p-IGF-1 and e-IGF-1 groups than the ACS group both 5 and 10weeks after surgery. Bone mineral density, bone volume, and bone surface values were also higher in the IGF groups than in the ACS group. Local delivery of p-IGF-1 or e-IGF-1 more greatly enhanced the expression of osteoblast-specific markers, but inhibited osteoclast formation, in newly formed bone compared with ACS control group. Specifically, p-IGF-1 treatment induced higher expression of alkaline phosphatase, osteocalcin, and osteopontin in the defect site than did e-IGF-1. Furthermore, treatment with p-IGF-1, but not e-IGF-1, increased mineralization of MC3T3-E1 cells, with the attendant upregulation of osteogenic marker genes. Collectively, our findings suggest the potential of p-IGF-1 in promoting the processes required for bone regeneration.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We explored bone regenerating potential of IGF-1 produced by a plant culture system. </LI> <LI> Local delivery of IGF-1 increased bone formation in calvarial defect model of mice. </LI> <LI> IGF-1 treatment stimulated the expression of osteogenic marker genes in the defect. </LI> <LI> The IGF-1 induced new bone formation similar to that did a commercial IGF-1. </LI> <LI> These results support a clinical usefulness of the IGF-1 in repairing bone defects. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

바이오매스 반탄화물과 SRF의 연소특성 비교

Poudel Jeeban,김준석,김진우,최병수,오세천 한국열환경공학회 2015 열환경공학 Vol.12 No.1

Base-promoted ring opening of 3-chlorooxindoles for the construction of 2-aminoarylthioates and their transformation to quinazolin-4(3<i>H</i>)-ones

Poudel, Tej Narayan,Khanal, Hari Datta,Lee, Yong Rok The Royal Society of Chemistry 2018 NEW JOURNAL OF CHEMISTRY Vol.42 No.6

<P>An unprecedented methodology for the preparation of diverse <I>S</I>-alkyl 2-aminoarylthioates has been developed based on cesium carbonate-promoted direct ring opening of 3-chlorooxindoles with thiols. This novel protocol proceeds <I>via</I> cascade sulfenylation, aerial oxidation, C2-C3 bond cleavage, and decarboxylation. The resulting products from this transformation exhibit potential for application as fluorescent sensors for the detection of Fe<SUP>3+</SUP> ions. In addition, this methodology provides a concise pathway for the construction of a variety of biologically interesting quinazolin-4(3<I>H</I>)-ones in good yields.</P>

A batch-by-batch free route for the continuous production of black phosphorus nanosheets for targeted combination cancer therapy

Poudel, Bijay Kumar,Hwang, Jungho,Ku, Sae Kwang,Kim, Jong Oh,Byeon, Jeong Hoon Nature Publishing Group 2018 NPG Asia Materials Vol.10 No.-

Efficient test to evaluate the consistency of elastic and viscous moduli with Kramers–Kronig relations

Poudel Sanjeeb,Shanbhag Sachin 한국유변학회 2022 Korea-Australia rheology journal Vol.34 No.4

The principle of causality constrains the real and imaginary parts of the complex modulus G∗ = G′ + iG′′ via Kramers– Kronig relations (KKR). Thus, the consistency of observed elastic or storage (G′) and viscous or loss (G′′) moduli can be ascertained by checking whether they obey KKR. This is important when master curves of the complex modulus are constructed by transforming a number of individual datasets; for example, during time-temperature superposition. We adapt a recently developed statistical technique called the ‘Sum of Maxwell Elements using Lasso’ or SMEL test to assess the KKR compliance of linear viscoelastic data. We validate this test by successfully using it on real and synthetic datasets that follow and violate KKR. The SMEL test is found to be both accurate and efficient. As a byproduct, the parameters inferred during the SMEL test provide a noisy estimate of the discrete relaxation spectrum. Strategies to improve the quality and interpretability of the extracted discrete spectrum are explored by appealing to the principle of parsimony to first reduce the number of parameters, and then to nonlinear regression to fi ne tune the spectrum. Comparisons with spectra obtained from the open-source program pyReSpect suggest possible tradeoff s between speed and accuracy.

Flavonoids from Triticum aestivum inhibit adipogenesis in 3T3-L1 cells by upregulating the insig pathway

POUDEL, BARUN,NEPALI, SARMILA,XIN, MINGJIE,KI, HYEON-HUI,KIM, YOUNG-HO,KIM, DAE-KI,LEE, YOUNG-MI SPANDIDOS PUBLICATIONS 2015 MOLECULAR MEDICINE REPORTS Vol.12 No.2

<P>The present study aimed to compare the potential anti-adipogenic effects and underlying mechanisms of the luteolin, isoscoparin and isoorientin flavonoids, purified from Triticum aestivum sprout (TA) in 3T3-L1 cells. The cells were treated with different concentrations of flavonoids for 8 days and the lipid accumulation was assessed using Oil-Red-O staining. The expression levels of the transcription factors and the genes involved in adipogenesis in the cells were assessed by reverse transcription-quantitative polymerase chain reaction and western blotting. The results demonstrated that 10 μM luteolin, isoscoparin or isoorientin inhibited lipid deposition in the cells by 74, 63 and 65%, respectively. The flavonoids also significantly inhibited the transcriptional regulators of adipogenesis, including peroxisome proliferator-activated receptor-γ, CAAT/enhancer binding protein-α and sterol regulatory element binding protein (SREBP)-1c, compared with the control cells. Similarly, there was a significant downregulation of the adipocyte specific markers associated with lipid metabolism, including activating protein-2, fatty acid synthase, hormone-sensitive lipase and lipoprotein lipase, in the flavonoid treated cells. Notably, the cells treated with the flavonoids demonstrated increased expression levels of the insulin-induced genes, insig-1 and insig-2, which may have inhibited the activation of the adipogenic transcription factor, SREBP, eventually leading to the inhibition of adipogenesis. Taken together, these results revealed that the flavonoids from TA possessed an inhibitory effect on adipogenesis through downregulation of adipogenic transcription factors and genes associated with lipid metabolism, and the upregulation of insig 1 and 2, suggesting that the flavonoids from TA may be potential therapeutic agents for the prevention and treatment of obesity.</P>