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한국인 정신분열병 환자의 지연성 운동장애와 $CYP2D6^*4$ 및 $CYP2D6^*10$ 다형성들의 연합에 대한 고찰
우성일,강동우,서한길,김봉조,이인상,정근화,박소영,정치영,이환철,정경천,손진욱,Woo, Sung-Il,Kang, Dong-Woo,Seo, Han-Gil,Kim, Bong-Jo,Lee, In-Sang,Jeong, Geun-Hoa,Park, So-Young,Jung, Chi-Yeong,Lee, Hwan-Cheol,Jeong, Kyeong-Cheon,Sohn, 대한생물정신의학회 2000 생물정신의학 Vol.7 No.2
P450 CYP2D6 enzyme(=debrisoquine hydroxylase) is known to metabolize many neuroleptics and some genetic polymorphisms in the CYP2D6 gene were reported to be associated with tardive dyskinesia(TD). We investigeted the association of two genetic polymorphisms in the CYP2D6 gene, $CYP2D6^*4$ and $CYP2D6^*10$, with TD in Korean schizophrenic subjects. Subjects consisted of 71 Korean schizophrenics and TD was evaluated using the Abnormal Involuntary Movement Scale (AIMS). There were no statistically significant differences in the demographic variables of age, male to female percentage and the current antipsychotic(CPZ equivalent) dose between the group with TD and the group without TD. But the duration of antipsychotic drug exposure was significantly higher in the group without TD(p=0.000, by independent t-test). The mean AIMS score in the group with TD was $11.2{\pm}6.6$(S.D.). Genotypings for the presence of $CYP2D6^*4$ and $CYP2D6^*10$ were done using PCR amplifications and endonuclease digestions. There were no statistically significant genotypic and alleleic associations between TD and $CYP2D6^*4$(by chisquare tests), and between TD and $CYP2D6^*10$(by chi-square tests). These results indicate that the $CYP2D6^*4$ and $CYP2D6^*10$ polymorphisms have no significant roles in the causation of TD.
Park, Chul-Hong,Son, Hyeong-U,Yoo, Chi-Yeol,Lee, Sang-Han TaylorFrancis 2017 Pharmaceutical biology Vol.55 No.1
<P><B>Abstract</B></P><P><B>Context:</B><I>Aloe</I> has been used for the prevention and cure of various diseases and symptoms including burns, injuries, oedema and pain.</P><P><B>Objective:</B> This study determines the specific inhibitory activity of matrix metalloproteinase (MMP)-9 induced by the low molecular-weight gel fraction of <I>Aloe vera</I> (L.) Burm.f. (lgfAv) on alcohol-induced acute gastric lesions.</P><P><B>Materials and methods:</B> We examined the protective effects of oral (p.o.) administration of lgfAv (molecular weight cutoff <50.0 kDa, 150.0 mg/kg body weight) in a Balb/c mouse model of alcohol-induced acute gastritis for 1 h exposure. By measuring ulcer index, we compared the antiulcerative activity of the fraction. mRNA expression and immunohistochemical analysis of various biomarkers were performed.</P><P><B>Results:</B> The lgfAv-treated mice exhibited drastically fewer ulcer lesions than the untreated control mice did. It featured that lgfAv lessened the ulcer lesions than their relevant controls. Moreover, the transcriptional level of MMP-9 was completely alleviated by lgfAv treatment in alcohol-treated gastritis-induced mice.</P><P><B>Discussion:</B> The transcriptional level of MMP-9 was significantly alleviated by lgfAv treatment of the model. However, reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry experiments revealed that lgfAv treatment in mucosal tissues had the potential to inhibit the mRNA and protein expression levels of MMP-9, respectively. The protein expression of MMP-9 was closely associated with lgfAv-induced gastroprotection against alcohol-induced gastric lesions.</P><P><B>Conclusions:</B> The present findings suggest that lgfAv has the potential to alleviate alcohol-induced acute gastric lesions, which is mediated in part, mainly by the suppression of the mRNA expression of MMP-9.</P>
Clinical proteomic analysis of scrub typhus infection
Park, Edmond Changkyun,Lee, Sang-Yeop,Yun, Sung Ho,Choi, Chi-Won,Lee, Hayoung,Song, Hyun Seok,Jun, Sangmi,Kim, Gun-Hwa,Lee, Chang-Seop,Kim, Seung Il BioMed Central 2018 Clinical proteomics Vol.15 No.1
<P><B>Background</B></P><P> Scrub typhus is an acute and febrile infectious disease caused by the Gram-negative α-proteobacterium <I>Orientia tsutsugamushi</I> from the family Rickettsiaceae that is widely distributed in Northern, Southern and Eastern Asia. In the present study, we analysed the serum proteome of scrub typhus patients to investigate specific clinical protein patterns in an attempt to explain pathophysiology and discover potential biomarkers of infection.</P><P><B>Methods</B></P><P>Serum samples were collected from three patients (before and after treatment with antibiotics) and three healthy subjects. One-dimensional sodium dodecyl sulphate–polyacrylamide gel electrophoresis followed by liquid chromatography-tandem mass spectrometry was performed to identify differentially abundant proteins using quantitative proteomic approaches. Bioinformatic analysis was then performed using Ingenuity Pathway Analysis.</P><P><B>Results</B></P><P>Proteomic analysis identified 236 serum proteins, of which 32 were differentially expressed in normal subjects, naive scrub typhus patients and patients treated with antibiotics. Comparative bioinformatic analysis of the identified proteins revealed up-regulation of proteins involved in immune responses, especially complement system, following infection with <I>O. tsutsugamushi</I>, and normal expression was largely rescued by antibiotic treatment.</P><P><B>Conclusions</B></P><P>This is the first proteomic study of clinical serum samples from scrub typhus patients. Proteomic analysis identified changes in protein expression upon infection with <I>O. tsutsugamushi</I> and following antibiotic treatment. Our results provide valuable information for further investigation of scrub typhus therapy and diagnosis.</P><P><B>Electronic supplementary material</B></P><P>The online version of this article (10.1186/s12014-018-9181-5) contains supplementary material, which is available to authorized users.</P>
PARK, JAE-HONG,OH, EUN-JIN,CHOI, YUNG HYUN,KANG, CHI-DUG,KANG, HO SUNG,KIM, DONG-KYOO,KANG, KWANG IL,YOO, MI-AE 부산대학교 유전공학연구소 2001 분자생물학 연구보 Vol.17 No.-
Previous studies have shown that dexamethasone, a synthetic glucocorticoid, can induce a G1 arrest, however, genistein, a natural isoflavonoid phytoestrogen, induces a G2/M arrest in the cell cycle progression in various cancer cell lines. A block of cell cycle checkpoint by dexamethasone and genistein correlates with a selective induction of cyclin-dependent kinase (Cdk) inhibitor p21^WAFI/CIPI in a tumor suppressor p53-independent manner and abolishment of Cdk2 phosphorylation. In the present study, the effects of dexamethasone and genistein (both singly and combined) on the expression of p21 in human hepatocellular Hep G2 and colorectal Colo320 HSR carcinoma cells were evaluated. Whereas dexamethasone mildly induced the level of p21 protein, genistein strongly increased the expression of p21 protein in our experimental condition. Both compounds also activated p21 promoter reporter constructs. The combined effects of dexamethasone and genistein on the induction of p21 protein and activation of p21 promoter were synergistic in both cell lines. These findings indicate that dexamethasone and genistein act in a synergistic fashion and have potential for combination chemotherapy for the treatment of liver and colon cancer.
Chi Hwan Choi,Won Dong Kim,Sang Jeon Lee,Woo-Yoon Park 대한방사선종양학회 2012 Radiation Oncology Journal Vol.30 No.3
Purpose: The aim of this study was to identify clinical predictive factors for tumor response after preoperative chemoradiotherapy (CRT) in rectal cancer. Materials and Methods: The study involved 51 patients who underwent preoperative CRT followed by surgery between January 2005 and February 2012. Radiotherapy was delivered to the whole pelvis at a dose of 45 Gy in 25 fractions, followed by a boost of 5.4 Gy in 3 fractions to the primary tumor with 5 fractions per week. Three different chemotherapy regimens were used (5-fl uorouracil and leucovorin, capecitabine, or tegafur/uracil). Tumor responses to preoperative CRT were assessed in terms of tumor downstaging and pathologic complete response (ypCR). Statistical analyses were performed to identify clinical factors associated with pathologic tumor response. Results: Tumor downstaging was observed in 28 patients (54.9%), whereas ypCR was observed in 6 patients (11.8%). Multivariate analysis found that predictors of downstaging was pretreatment relative lymphocyte count (p = 0.023) and that none of clinical factors was significantly associated with ypCR. Conclusion: Pretreatment relative lymphocyte count (%) has a significant impact on the pathologic tumor response (tumor downstaging) after preoperative CRT for locally advanced rectal cancer. Enhancement of lymphocyte-mediated immune reactions may improve the effect of preoperative CRT for rectal cance.
Enhancement of mass transport in fuel cells using three-dimensional graphene foam as flow field
Park, Ji Eun,Lim, Jongkoo,Kim, Sungjun,Choi, Insoo,Ahn, Chi-Yeong,Hwang, Wonchan,Lim, Myung Su,Cho, Yong-Hun,Sung, Yung-Eun Elsevier 2018 ELECTROCHIMICA ACTA Vol.265 No.-
<P><B>Abstract</B></P> <P>Graphene foam is a three-dimensional graphene-based material with interconnected macropores and it combines the advantage of graphene and structural characteristics of metal foam. Various kinds of metal foam have been developed as flow fields because their high porosity distributes reactants in an entire area and removes generated water. However, metal foam is highly susceptible to corrosion under the operating conditions of polymer-electrolyte-membrane fuel cells. In this work, we proposed using graphene foam as a flow field to investigate its effect on enhancing mass transport of reactants and products. Single-cell tests of the graphene-foam flow field showed the enhancement of mass transport, which led to increased performance at high current densities. Measurements of the oxygen gain (i.e., the difference in voltage under O<SUB>2</SUB> and air atmospheres), electrochemical impedance spectra, and simulation results also revealed that the graphene-foam membrane-electrode assembly (MEA) exhibited lower mass-transport resistance than a conventional MEA because graphene foam is advantageous for the mass transport of reactants and water.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A graphene foam MEA using graphene foam as flow field is proposed. </LI> <LI> The graphene foam MEA exhibits high cell performance, especially at high current densities. </LI> <LI> The graphene foam as flow field can decrease mass transport resistance. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Chi, Cheng,Giri, Sib Sankar,Jun, Jin Woo,Kim, Hyoun Joong,Kim, Sang Wha,Yun, Saekil,Park, Se Chang Elsevier 2017 FISH AND SHELLFISH IMMUNOLOGY Vol.65 No.-
<P><B>Abstract</B></P> <P>Okadaic acid (OA) is produced by dinoflagellates during harmful algal blooms and is a diarrhetic shellfish-poisoning (DSP) toxin. This toxin is particularly problematic for bivalves that are cultured for human consumption. This study aimed to reveal the effects of exposure to OA on the non-specific immune responses of bay scallop, <I>Argopecten irradians</I>. Various immunological parameters (superoxide dismutase (SOD), acid phosphatase (ACP), alkaline phosphatase (ALP), lysozyme activities, and total protein level) were assessed in the hemolymph of bay scallops at 3, 6, 12, 24, and 48 h post-exposure (hpe) to different concentrations (50, 100, and 500 nM) of OA. Moreover, the expression of immune system-related genes (<I>MnSOD</I>, <I>PrxV</I>, <I>PGRP</I>, and <I>BD</I>) was also measured. Results showed that SOD and ACP activities were decreased between 12 and 48 hpe. The ALP, lysozyme activities, and total protein levels were also modulated after exposure to different concentrations of OA. The expression of immune-system-related genes was also assessed at different time points during the exposure period. Overall, our results suggest that the exposure to OA had negative effects on the antioxidant and non-specific immune responses, and even disrupted the metabolism of bay scallops, making them more vulnerable to environmental stress-inducing agents; they provide a better understanding of the response status of bivalves against DSP toxins.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Effect of okadaic acid (OA) on the bay scallop was investigated. </LI> <LI> Cytokine responses of bay scallop were altered by OA. </LI> <LI> Immune and antioxidant responses to OA were time and concentration-dependent. </LI> </UL> </P>