Effect on the Antioxidant Capacity of Polypropylene by Vanillin: A Natural Antioxidant Extracted from Cotton Stalk Lignin

Mingyu He,Han Dong,Mamatjan Yimit 한국고분자학회 2021 폴리머 Vol.45 No.5

Vanillin (VA) was extracted from cotton stalk lignin by UV photocatalysis method using Co<SUP>2+</SUP>/Ni<SUP>2+</SUP> as a catalyst, the yield of which was quantitative analyzed through gas chromatography-mass spectrometer and high performance liquid chromatography. The structure and thermal properties were compared between extracted and standard VA, which showed basically consistent. PP/VA materials characterized by fourier transform infrared spectroscopy were prepared by melt intercalation and subjected to 20 days thermal oxygen aging. Thermal gravimetric analysis and oxidation induction time results showed VA (0.9 wt%) could improve the thermal stability of polypropylene (PP) by 33.4 ℃ and 49.03%. Differential scanning calorimetry confirmed that VA increased the crystallinity of PP reaching to 15.70%. After 20 days aging, mechanical test showed the impact strength and breaking elongation retention of PV-3 (0.9 wt%) were 10.0% and 14.8% higher than those of PP; surface scanning electron microscopy showed that PV-3 had the most smooth surface.

Carbon Source Affects Synthesis, Structures, and Activities of Mycelial Polysaccharides from Medicinal Fungus Inonotus obliquus

( Huihui He ),( Yingying Li ),( Mingyue Fang ),( Tiantian Li ),( Yunxiang Liang ),( Yuxia Mei ) 한국미생물생명공학회(구 한국산업미생물학회) 2021 Journal of microbiology and biotechnology Vol.31 No.6

The effects of various carbon sources on mycelial growth and polysaccharide synthesis of the medicinal fungus Inonotus obliquus in liquid fermentation were investigated. After 12-d fermentation, mycelial biomass, polysaccharide yield, and polysaccharide content were significantly higher in Glc+Lac group (glucose and lactose used as combined carbon source) than in other groups. Crude polysaccharides (CIOPs) and the derivative neutral polysaccharides (NIOPs) were obtained from mycelia fermented using Glc, fructose (Fru), Lac, or Glc+Lac as carbon source. Molecular weights of four NIOPs (termed as NIOPG, NIOPF, NIOPL, and NIOPGL) were respectively 780.90, 1105.00, 25.32, and 10.28 kDa. Monosaccharide composition analyses revealed that NIOPs were composed of Glc, Man, and Gal at different molar ratios. The NIOPs were classified as α-type heteropolysaccharides with 1→2, 1→3, 1→4, 1→6 linkages in differing proportions. In in vitro cell proliferation assays, viability of RAW264.7 macrophages was more strongly enhanced by NIOPL or NIOPGL than by NIOPG or NIOPF, and proliferation of HeLa or S180 tumor cells was more strongly inhibited by NIOPG or NIOPGL than by NIOPF or NIOPL, indicating that immune-enhancing and antitumor activities of NIOPs were substantially affected by carbon source. qRT-PCR analysis revealed that expression levels of phosphoglucose isomerase (PGI) and UDP-Glc 4-epimerase (UGE), two key genes involved in polysaccharide synthesis, varied depending on carbon source. Our findings, taken together, clearly demonstrate that carbon source plays an essential role in determining structure and activities of I. obliquus polysaccharides by regulating expression of key genes in polysaccharide biosynthetic pathway.

Efficiency of gerAa, tupA and ca transformation in Bacillus subtilis for self-healing of concrete cracks

Hanxing He,Gefei Li,Jiantao Zhang,Jinlong Zhang,Mingyue Luo,Wenkai Hu,Yamin Lin,Ziyu Deng,Zhicheng Liu,Weizhao Chen,Xu Deng 한양대학교 세라믹연구소 2019 Journal of Ceramic Processing Research Vol.20 No.5

A genetic approach was proposed to modify the characteristics of Bacillus subtilis strain WB800 (B. subtilis WB800) for selfhealing of concrete cracks. Three genes, namely gerAa which encodes germination receptors activated by L-alanine, tupA which is responsible for the synthesis of teichuronopeptide, and ca which encodes carbonic anhydrase (CA) catalyzing the synthesis of carbonate ion, were separately transformed into WB800. To protect bacterial cells from being squeezed, microspheres were produced with microcrystal cellulose (MCC) before the introduction of bacteria into the specimens. The results showed that the modified B. subtilis expressing GerA achieved 39.9% of germination ratio compared to 17% by the original host cells. With the transformation of tupA, the modified strain demonstrated higher resistance to alkaline environments, tolerating pH as high as 11, while the original strain only tolerated pH 9. The modified strain expressing CA induced more calcium carbonate than the original cells. Energy dispersive spectroscopy (EDS) identified the produced precipitate to be calcite (CaCO3). Moreover, a mathematical model was developed to optimize the influential factors of calcium precipitation process. Finally, based on the above results, an effective self-healing of concrete crack was achieved. This study may provide a promising strategy to improve the efficiency of bacterial self-healing of concrete cracks.

Locating and identifying model-free structural nonlinearities and systems using incomplete measured structural responses

Lijun Liu,Ying Lei,Mingyu He 국제구조공학회 2015 Smart Structures and Systems, An International Jou Vol.15 No.2

Structural nonlinearity is a common phenomenon encountered in engineering structures under severe dynamic loading. It is necessary to localize and identify structural nonlinearities using structural dynamic measurements for damage detection and performance evaluation of structures. However, identification of nonlinear structural systems is a difficult task, especially when proper mathematical models for structural nonlinear behaviors are not available. In prior studies on nonparametric identification of nonlinear structures, the locations of structural nonlinearities are usually assumed known and all structural responses are measured. In this paper, an identification algorithm is proposed for locating and identifying model-free structural nonlinearities and systems using incomplete measurements of structural responses. First, equivalent linear structural systems are established and identified by the extended Kalman filter (EKF). The locations of structural nonlinearities are identified. Then, the model-free structural nonlinear restoring forces are approximated by power series polynomial models. The unscented Kalman filter (UKF) is utilized to identify structural nonlinear restoring forces and structural systems. Both numerical simulation examples and experimental test of a multi-story shear building with a MR damper are used to validate the proposed algorithm.

Prevalence of Allergic Rhinitis Among Adults in Urban and Rural Areas of China: A Population-Based Cross-Sectional Survey

Ming Zheng,Xiangdong Wang,Mingyu Bo,Kuiji Wang,Yan Zhao,Fei He,Feifei Cao,Luo Zhang,Claus Bachert 대한천식알레르기학회 2015 Allergy, Asthma & Immunology Research Vol.7 No.2

Purpose: The aim of the present study was to compare the prevalence of self-reported and confirmable allergic rhinitis (AR) with positive skin pricktest (SPT) results among adults living in urban and rural areas of China. Methods: Adults from a community in Beijing and a village in Baoding wereselected as representative urban and rural dwellers, respectively. All eligible residents were enrolled from the population register and received aface-to-face interview using modified validated questionnaires. Equal sets of randomly selected self-reporting AR-positive and AR-negative participantswho responded to the questionnaires were also investigated using skin prick tests. Results: A total of 803 participants in the rural area and atotal of 1,499 participants in the urban area completed the questionnaires, with response rates being 75.9% and 81.5% respectively. The prevalenceof self-reported AR of the rural area (19.1%) was significantly higher than that of the urban area (13.5%). The elementary school of educational levelincreased the risk of having AR (adjusted OR=2.198, 95% CI=1.072-2.236) .The positive SPT rates among subjects with self-reported AR in therural and urban areas were 32.5% and 53.3%, respectively; the confirmable AR prevalence of 6.2% and 7.2% among the rural and urban adults, respectively. Conclusions: The prevalence of confirmable AR is similar between rural and urban areas in China, although there is a higher prevalenceof self-reported AR in the former. Purpose: The aim of the present study was to compare the prevalence of self-reported and confirmable allergic rhinitis (AR) with positive skin prick test (SPT) results among adults living in urban and rural areas of China. Methods: Adults from a community in Beijing and a village in Baoding were selected as representative urban and rural dwellers, respectively. All eligible residents were enrolled from the population register and received a face-to-face interview using modified validated questionnaires. Equal sets of randomly selected self-reporting AR-positive and AR-negative participants who responded to the questionnaires were also investigated using skin prick tests. Results: A total of 803 participants in the rural area and a total of 1,499 participants in the urban area completed the questionnaires, with response rates being 75.9% and 81.5% respectively. The prevalence of self-reported AR of the rural area (19.1%) was significantly higher than that of the urban area (13.5%). The elementary school of educational level increased the risk of having AR (adjusted OR=2.198, 95% CI=1.072-2.236) .The positive SPT rates among subjects with self-reported AR in the rural and urban areas were 32.5% and 53.3%, respectively; the confirmable AR prevalence of 6.2% and 7.2% among the rural and urban adults, respectively. Conclusions: The prevalence of confirmable AR is similar between rural and urban areas in China, although there is a higher prevalence of self-reported AR in the former.

miR-148b-3p, miR-190b, and miR-429 Regulate Cell Progression and Act as Potential Biomarkers for Breast Cancer

Wenzhu Dai,Jixiang He,Ling Zheng,Mingyu Bi,Fei Hu,Minju Chen,Heng Niu,Jingyu Yang,Ying Luo,Wenru Tang,Miaomiao Sheng 한국유방암학회 2019 Journal of breast cancer Vol.22 No.2

Purpose: Breast cancer is the most frequently diagnosed malignancy in women worldwide. MicroRNAs (miRNAs) are thought to serve as potential biomarkers in various cancers, including breast cancer. Methods: We evaluated the miRNA expression profiles in 1,083 breast cancer samples and 104 normal breast tissues from The Cancer Genome Atlas database. We used the edgeR package of R software to analyze the differentially expressed miRNAs in normal and cancer tissues, and screened survival-related miRNAs by Kaplan-Meier analysis. A receiver operating characteristic curve was generated to evaluate the accuracy of these miRNAs as molecular markers for breast cancer diagnosis. Furthermore, the functional role of these miRNAs was verified using cell experiments. Targets of candidate miRNAs were predicted using 9 online databases, and Gene Ontology (GO) functional annotation and pathway analyses were conducted using Database for Annotation, Visualization and Integrated Discovery online tool. Results: A total of 68 miRNAs showed significantly different expression patterns between the groups (p < 0.001), and 13 of these miRNAs were significantly associated with poor survival (p < 0.05). Three miRNAs with high specificity and sensitivity, namely, miR-148b-3p, miR-190b, and miR-429, were selected. In vitro experiments showed that the overexpression of these 3 miRNAs significantly promoted the proliferation and migration of MDA-MB-468 and T47D cells and reduced the apoptosis of T47D cells. GO and pathway enrichment analyses revealed that the targets of these dysregulated miRNAs were involved in many critical cancer-related biological processes and pathways. Conclusion: The miR-148b-3p, miR-190b, and miR-429 may serve as potential diagnostic and prognostic markers for breast cancer. This study demonstrated the roles of these 3 miRNAs in the initiation and progression of breast cancer.

Locating and identifying model-free structural nonlinearities and systems using incomplete measured structural responses

Liu, Lijun,Lei, Ying,He, Mingyu Techno-Press 2015 Smart Structures and Systems, An International Jou Vol.15 No.2

Structural nonlinearity is a common phenomenon encountered in engineering structures under severe dynamic loading. It is necessary to localize and identify structural nonlinearities using structural dynamic measurements for damage detection and performance evaluation of structures. However, identification of nonlinear structural systems is a difficult task, especially when proper mathematical models for structural nonlinear behaviors are not available. In prior studies on nonparametric identification of nonlinear structures, the locations of structural nonlinearities are usually assumed known and all structural responses are measured. In this paper, an identification algorithm is proposed for locating and identifying model-free structural nonlinearities and systems using incomplete measurements of structural responses. First, equivalent linear structural systems are established and identified by the extended Kalman filter (EKF). The locations of structural nonlinearities are identified. Then, the model-free structural nonlinear restoring forces are approximated by power series polynomial models. The unscented Kalman filter (UKF) is utilized to identify structural nonlinear restoring forces and structural systems. Both numerical simulation examples and experimental test of a multi-story shear building with a MR damper are used to validate the proposed algorithm.

Surface Molecularly Imprinted Polymers Based on NH2-MIL-53 for Selective Extraction Ochratoxin A in Real Sample

Xing Zhang,Juan He,Huige Wang,Pengfei Xu,Mingyu Wang,Yuanyuan Li,Jing Chen,Lijun He 한국고분자학회 2022 Macromolecular Research Vol.30 No.10

In this study, surface molecularly imprinted polymers were synthesized with the metal-organic framework NH2-MIL-53 as carrier and naringenin as alternative template of Ochratoxin A (OTA). The amino groups on the surface of NH2-MIL- 53 reacted with the epoxy groups in glycidyl methacrylate (GMA). Subsequently, the double bonds of the GMA grafting on the surface of the material were used for polymerization to prepare the surface molecularly imprinted polymers. The obtained polymers were characterized by scanning electron microscope, transmission electron microscope, energy spectrometer, Fourier infrared spectrometer, X-ray diffraction, and laser particle size analyzer. Synthetic polymer was combined with solid phase extraction for separation and enrichment of trace OTA in grains. The spiked recoveries were found to be 78.95-106.45%, with relative standard deviations ranging from 5.16% to 6.31%. The detection limit and the quantification limit of the method were 0.04 μg·kg-1 and 0.12 μg·kg-1, respectively. Comparing the pretreatment effects of the self-made cartridge and the immunoaffinity column on the OTA in the sample, it showed that the self-made column had excellent performance and provided a new pretreatment method for the treatment of OTA in grains.

Detection and parametric identification of structural nonlinear restoring forces from partial measurements of structural responses

Lei, Ying,Hua, Wei,Luo, Sujuan,He, Mingyu Techno-Press 2015 Structural Engineering and Mechanics, An Int'l Jou Vol.54 No.2

Compared with the identification of linear structures, it is more challenging to conduct identification of nonlinear structure systems, especially when the locations of structural nonlinearities are not clear in structural systems. Moreover, it is highly desirable to develop methods of parametric identification using partial measurements of structural responses for practical application. To cope with these issues, an identification method is proposed in this paper for the detection and parametric identification of structural nonlinear restoring forces using only partial measurements of structural responses. First, an equivalent linear structural system is proposed for a nonlinear structure and the locations of structural nonlinearities are detected. Then, the parameters of structural nonlinear restoring forces at the locations of identified structural nonlinearities together with the linear part structural parameters are identified by the extended Kalman filter. The proposed method simplifies the identification of nonlinear structures. Numerical examples of the identification of two nonlinear multi-story shear frames and a planar nonlinear truss with different nonlinear models and locations are used to validate the proposed method.

State of Charge Estimation for Li-Ion Batteries Based on an Unscented H-Infi nity Filter

Yuanyuan Liu,Tiantian Cai,Jingbiao Liu,Mingyu Gao,Zhiwei He 대한전기학회 2020 Journal of Electrical Engineering & Technology Vol.15 No.6

The state of charge (SOC) of lithium-ion batteries refl ects their remaining capacity. Accurate estimation of SOC helps battery safety and is benefi cial to the effi cient management of batteries. The charging and discharging processes of lithium-Ion batteries are very complicated, and it is diffi cult to obtain accurate SOC estimation results. Therefore, it is important to study improved algorithms for SOC estimation for this nonlinear non-Gaussian battery system. In this paper, we propose an unscented H-infi nity fi lter (UHF) based SOC estimation method, which combines the advantages of both the unscented Kalman fi lter (UKF) and the H-infi nity fi lter (HF). The UKF propagates the sigma points through the nonlinear system and does not need the fi rst-order linear approximation of the system equation, while the HF can suppress the non-Gaussian noise in the system to the greatest extent. The proposed UHF based SOC estimation algorithm is verifi ed and evaluated in the battery management system, and further optimized in practical problems. Experimental results show that the proposed UHF based algorithm can perform accurate SOC estimation for lithium-ion batteries, and is superior to the UKF based SOC estimation.