Detection of Genotoxic, Cytotoxic, and Protective Activities of Eugenia dysenterica DC. (Myrtaceae) in Mice

Pabline Marinho Vieira,Eduardo Veronezi,Carolina R. Silva,Lee Chen-Chen 한국식품영양과학회 2012 Journal of medicinal food Vol.15 No.6

Eugenia dysenterica DC. (Myrtaceae), popularly known in Brazil as cagaiteira, is a widespread plant species in the Brazilian Cerrado. In folk medicine, the leaves of this plant are used to treat diarrhea and dysentery. The fruits are used for fresh consumption and industrial purposes. Because of the use of this plant as a therapeutic resource and food, the present study evaluated the genotoxic, cytotoxic, antigenotoxic, and anticytotoxic effects of the lyophilized ethanolic leaf extract of E. dysenterica using the mouse bone marrow micronucleus test. The genotoxicity and antigenotoxicity of this extract were evaluated using the frequency of micronucleated polychromatic erythrocytes, and the cytotoxicity and anticytotoxicity were assessed by the polychromatic and normochromatic erythrocyte ratio. According to our results, the lyophilized ethanolic leaf extract of E. dysenterica exhibited genotoxic and cytotoxic effects at the higher doses and protection against cyclophosphamideinduced genotoxic and cytotoxic actions at all doses tested.

Assessment of the Genotoxic, Antigenotoxic, and Cytotoxic Activities of the Ethanolic Fruit Extract of Solanum lycocarpum A. St. Hill. (Solanaceae) by Micronucleus Test in Mice

Pabline Marinho Vieira,Paula Moiana da Costa,Lee Chen-Chen 한국식품영양과학회 2010 Journal of medicinal food Vol.13 No.6

Solanum lycocarpum A. St. Hill. (Family Solanaceae), popularly known in Brazil as lobeira, is a common weed in the Brazilian Cerrado vegetation. The fruits of this species have been used in Brazil for culinary purposes and in folk medicine as a sedative, diuretic, antiepileptic, antispasmodic, hypoglycemic, and hypocholesterolemic agent as well as in the control of obesity. Due to the spreading use of this plant as a therapeutic resource and food, the present study aimed to evaluate the genotoxic, antigenotoxic, and cytotoxic effects of S. lycocarpum ethanolic fruit extract using the mouse bone marrow micronucleus test. Both genotoxicity and antigenotoxicity of this ethanolic fruit extract were evaluated by using the frequency of micronucleated polychromatic erythrocytes, whereas cytotoxicity was assessed by the polychromatic and normochromatic erythrocytes ratio. Our results indicated that although S. lycocarpum ethanolic fruit extract did not exhibit genotoxic effect in mice bone marrow, both cytotoxic and antigenotoxic actions were evidenced at all tested doses.

Solanum paniculatum L. Leaf and Fruit Extracts: Assessment of Modulation of Cytotoxicity and Genotoxicity by Micronucleus Test in Mice

Pabline Marinho Vieira,Jose Realino Paula,Lee Chen-Chen 한국식품영양과학회 2010 Journal of medicinal food Vol.13 No.6

Solanum paniculatum L. is a plant species widespread throughout tropical America, especially in the Brazilian Savanna region. It is used in Brazil for culinary purposes and in folk medicine to treat liver and gastric dysfunctions, as well as hangovers. Previous studies with S. paniculatum ethanolic leaf extract (ELE) or ethanolic fruit extract (EFE) demonstrated that they have no genotoxic activity meant either in the micronucleus test in mice or in the phage induction SOS Inductest in bacterial strains; however, cytotoxicity was demonstrated in both tests. Because of the spread use of this plant as a therapeutic resource and food, the present study aimed at evaluating the modulator effects of S. paniculatum ELE or EFE against mitomycin C (MMC) using the mouse bone marrow micronucleus test. This short-term test was used to detect the acute effects of responsive erythropoiesis after 24- and 48-hour exposure periods. Swiss-Webster mice were orally treated with three different concentrations (100, 200, or 300mg/kg) of ELE or EFE simultaneously with a single dose of MMC (4mg/kg i.p.). Antigenotoxicity was evaluated using the frequency of micronucleated polychromatic erythrocytes (MNPCEs), whereas anticytotoxicity was assessed by the polychromatic/normochromatic erythrocyte ratio. Our results demonstrated that neither the ELE nor EFE of S. paniculatum protected cells against the cytotoxic action of MMC. Nevertheless, the present study showed the antimutagenic effect of ELE after a 24-hour treatment (reduction in the frequencies of MNPCEs after a 48-hour treatment with ELE can be due to toxicity) and no antimutagenic action of the EFE treatment against the aneugenic and/or clastogenic activities of MMC.

Assessment of the mechanical properties of the muscle-tendon unit by supersonic shear wave imaging elastography: a review

Kelly Mônica Marinho e Lima,José Francisco Silva Costa Júnior,Wagner Coelho de Albuquerque Pereira,Liliam Fernandes de Oliveira 대한초음파의학회 2018 ULTRASONOGRAPHY Vol.37 No.1

This review aimed to describe the state of the art in muscle-tendon unit (MTU) assessment by supersonic shear wave imaging (SSI) elastography in states of muscle contraction and stretching, during aging, and in response to injury and therapeutic interventions. A consensus exists that MTU elasticity increases during passive stretching or contraction, and decreases after static stretching, electrostimulation, massage, and dry needling. There is currently no agreement regarding changes in the MTU due to aging and injury. Currently, the application of SSI for the purpose of diagnosis, rehabilitation, and physical training remains limited by a number of issues, including the lack of normative value ranges, the lack of consensus regarding the appropriate terminology, and an inadequate understanding of the main technical limitations of this novel technology

A dual-functional gadolinium-conjugated hydroxyapatite nanoparticle-containing curcumin as a potential nanoplatform for bioapplications

Jéssica Pauline Nunes Marinho,Marcelo Fernandes Cipreste,Alisson Krohling,Edésia Martins Barros Sousa 한양대학교 청정에너지연구소 2023 Journal of Ceramic Processing Research Vol.24 No.6

Nanomaterials such as hydroxyapatite have been extensively researched, thanks to their unsual properties, which are similarin chemical composition to bone tissue. In addition, hydroxyapatite nanoparticles offer a wide range of possibilities forfunctionalization which make it an interesting material for target drug delivery applications, showing strong potential forcoupling natural substances such as curcumin to improve the antitumor effect of this substance in the fight against cancer. Curcumin has been researched over the years, thanks to its anticancer properties. Hydroxyapatite also allows the incorporationof paramagnetic elements into their structure, such as gadolinium (Gd), allowing the production of a probe for diagnosticimaging using nuclear magnetic resonance imaging systems. In this work, hydroxyapatite nanorods (HA) doped withGd were synthesized to produce folic acid (FA) functionalized nanostructures for targeted delivery of curcumin (CM). Thesamples were characterized using various techniques, including Fourier transform infrared spectroscopy (FTIR), UV-visiblespectroscopy (UV-Vis), X-ray diffraction (XRD), thermogravimetric analysis (TGA), zeta potential analysis (ζ), transmissionelectron microscopy (TEM), and vibrating samples magnetometry (VSM). The results indicated a successful obtaining ofparamagnetic HA-Gd nanostructures functionalized with FA and CM, exhibiting characteristics that credit this system forfuture biological assays in order to evaluate their efficiency in the treatment and diagnosis of osteosarcoma

Anchor Bolts – Influence of Supplementary Reinforcement

L. M. Trautwein,A. M. Marinho,R. B. Gomes 대한토목학회 2018 KSCE JOURNAL OF CIVIL ENGINEERING Vol.22 No.2

An anchoring system can be loaded by tensile or shear stresses or a combination of these two; this study was limited to anchors subjected to tensile stresses. In this study, 33 anchor bolts, which were subjected to tensile stresses and divided into four series, were tested. The following are the primary variables tested: diameter, number of hooks, and number of supplementary reinforcement layers. The results obtained from the anchoring tests are shown, such as the failure mode and load, vertical displacements, and strains in the reinforcement bars. The results show that the anchoring strength increases with the presence of supplementaryreinforcement.

Effectiveness of Acupuncture in Dental Surgery: A Randomized, Crossover, Controlled Trial

Maria LB. Gil,Luide M.R.F. Marinho,Marcio de Moraes,Ronaldo S. Wada,Francisco C. Groppo,Jorge E. Sato,Maria L.R. de Sousa 사단법인약침학회 2020 Journal of Acupuncture & Meridian Studies Vol.13 No.3

Objectives: The objective of this crossover clinical study was to evaluate the effectiveness of Energy Regulation with Acupuncture in clinical occurrences in impacted lower third molar surgeries. Methods: The sample consisted of 22 patients with two impacted third molars, in symmetrical position; divided into two groups: Test Group (TG) with Real Energy Regulation Group and Sham Group (SG) with Acupuncture without Energy Regulation function. The extraction was performed 30 days apart. Energy flow (Ryodoraku Method) and energy regulation performed before extraction were measured. Heart Rate (HR) and Blood Pressure (BP) were evaluated before and after energy regulation and after surgery, residual edema was measured by facial measurements (angle of the mandible to tragus (A-T); angle of the mandible to labial commissure (A-LC); angle of the mandible to the wing of the nose (A-WN); angle of the mandible to the corner of the eye (A-CE); angle of the mandible to the chin (A-C); and mouth opening by the interincisal distance, before and after seven days of surgery. To quantify intraoperative bleeding (ml), blood was aspirated along with the saline solution using a portable vacuum pump adaptor. The amount of saline solution used was subtracted from the final amount of aspirated fluid. Results: Mean of bleeding was lower in TG (p = 0.0392). There were significant differences between groups in facial distances: A-LC (p = 0.010), A-WN (p = 0.030) and A-C (p = 0.008). Conclusion: Energy regulation with real acupuncture was effective in reducing postoperative residual edema and intraoperative bleeding.

Poster Session : PS 0012 ; Immunology : Retroperitoneal Fibrosis Related to Periaortitis - When to Think of It?

( Helena Lobo Martns ),( Nadejda Potlog ),( Antonio Marinho ),( Isabel Almeida ),( Carlos Vasconcelos ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Retroperitoneal fi brosis (RF) is a rare condition characterized by the presence of infl ammatory and fi brous retroperitoneal tissue that may be idiopathic or secondary to other causes. Idiopathic RF is part of the spectrum of chronic periaortitis, a condition characterized by infl ammation and fi brosis surrounding the aorta and iliac arteries. The pathogenesis of idiopathic retroperitoneal fi brosis is unclear. The authors report the case of a 66 year-old woman with RF diagnosis related to infrarenal infl ammatory aortitis in 2009, following the study of a hypochromic microcytic anemia and increased infl ammatory parameters, confi rmed by imaging. She has a history of stenosing ileal Crohn`s disease, underwent surgery in 2006, with recurrence of ileal disease two years ago, treated and currently stable. As other relevant pathological antecedents, she contacted patients with pulmonary tuberculosis. She starting anti-tuberculosis therapeutic in September 2009 for nine months, because she has positive Mantoux test. The patient began high-dose corticosteroids with clinical benefi t, however with reduction in dose to about 20mg/day clinical worsening was observed with marked increase in infl ammatory parameters. Azathioprine was added during a short period due to myelosuppression. Subsequently started methotrexate with good response at an early stage but with need to change to cyclosporine due to worsening of infl ammatory parameters. Although there was an initial response to this drug, a sustained analytical and clinical response could only be achieved through the introduction of anti-TNF-alpha (Infl iximab). It was systematically excluded neoplastic, infectious, autoimmune and other infl ammatory diseases. The authors argue the importance of knowing and diagnosing rare diseases such as retroperitoneal fi brosis. It presents insidiously, often making the diagnosis diffi cult, thus you need to think about these diseases for successful diagnose. We point out the importance of knowing the biological drugs as potential therapeutic weapons in these cases.

Mannosylerythritol lipids ameliorate ultraviolet A-induced aquaporin-3 downregulation by suppressing c-Jun N-terminal kinase phosphorylation in cultured human keratinocytes

배일홍,이성훈,오수정,최형원,Paulo A. Marinho,유재원,고재영,이은수,이태룡,이창석,김대용 대한약리학회 2019 The Korean Journal of Physiology & Pharmacology Vol.23 No.2

Mannosylerythritol lipids (MELs) are glycolipids and have several pharmacological efficacies. MELs also show skin-moisturizing efficacy through a yetunknown underlying mechanism. Aquaporin-3 (AQP3) is a membrane protein that contributes to the water homeostasis of the epidermis, and decreased AQP3 expression following ultraviolet (UV)-irradiation of the skin is associated with reduced skin moisture. No previous study has examined whether the skin-moisturizing effect of MELs might act through the modulation of AQP3 expression. Here, we report for the first time that MELs ameliorate the UVA-induced downregulation of AQP3 in cultured human epidermal keratinocytes (HaCaT keratinocytes). Our results revealed that UVA irradiation decreases AQP3 expression at the protein and messenger RNA (mRNA) levels, but that MEL treatment significantly ameliorated these effects. Our mitogenactivated protein kinase inhibitor analysis revealed that phosphorylation of c-Jun N-terminal kinase (JNK), but not extracellular signal-regulated kinase or p38, mediates UVA-induced AQP3 downregulation, and that MEL treatment significantly suppressed the UVA-induced phosphorylation of JNK. To explore a possible mechanism, we tested whether MELs could regulate the expression of peroxidase proliferatoractivated receptor gamma (PPAR-), which acts as a potent transcription factor for AQP3 expression. Interestingly, UVA irradiation significantly inhibited the mRNA expression of PPAR- in HaCaT keratinocytes, whereas a JNK inhibitor and MELs significantly rescued this effect. Taken together, these findings suggest that MELs ameliorate UVA-induced AQP3 downregulation in HaCaT keratinocytes by suppressing JNK activation to block the decrease of PPAR-. Collectively, our findings suggest that MELs can be used as a potential ingredient that modulates AQP3 expression to improve skin moisturization following UVA irradiation-induced damage.

Effects of the Use of Curcumin on Ulcerative Colitis and Crohn's Disease: A Systematic Review

Ricardo de Alvares Goulart,Sandra M. Barbalho,Vinícius Marinho Lima,Gabriela Achete de Souza,Julia Novaes Matias,Adriano Cressoni Araújo,Cláudio José Rubira,Rogério Leone Buchaim,Daniela Vieira Buchai 한국식품영양과학회 2021 Journal of medicinal food Vol.24 No.7

Inflammatory bowel disease (IBD) is an umbrella term used to describe chronic inflammatory disorders related to a substantial reduction in the quality of life of patients. Some patients with Crohn's disease (CD) and ulcerative colitis (UC) are refractory to conventional therapies, and Curcuma longa derivatives have been considered as adjuvants. Owing to the anti-inflammatory and antioxidant effects, some clinical trials used this plant in the therapeutic approach of IBD, and some meta-analyses evaluated the outcomes found in these studies. Owing to controversial findings, our systematic review aimed to evaluate these studies to show whether C. longa compounds can still be considered in the therapeutic approach of patients with CD and UC. MEDLINE-PubMed, EMBASE, and Cochrane were searched, and Preferred Reporting Items for a Systematic Review and Meta-Analysis guidelines were followed. The results of the randomized clinical trials (RCTs) showed promising results with the use of curcumin in the therapeutic approach of both UC and CD patients. Some meta-analyses show controversial results, possibly due to the presence of bias in the included studies. The actions of curcumin are achieved by several mechanisms, such as reducing the expression of interleukin (IL)-1, IL-6, IL-12, and tumor necrosis factor-α. Moreover, it reduces the levels of reactive oxygen species, such as superoxide anions and malondialdehyde. The results of using curcumin in CD and UC patients are challenging to be evaluated because RCTs are variable in the dose and the formulations of curcumin, in the time of treatment, and the route of administration. The number of patients in the samples is also usually small.