Array-based Nano-amplification Technique Was Applied in Detection of Hepatitis E Virus

( Hui Hui Liu ),( Xuan Cao ),( Yong Yang ),( Ming Gui Liu ),( Ye Fu Wang ) 생화학분자생물학회 2006 BMB Reports Vol.39 No.3

Array-based Nano-amplification Technique Was Applied in Detection of Hepatitis E Virus

Liu, Hui-Hui,Cao, Xuan,Yang, Yong,Liu, Ming-Gui,Wang, Ye-Fu Korean Society for Biochemistry and Molecular Biol 2006 Journal of biochemistry and molecular biology Vol.39 No.3

A rapid method for the detection of Hepatitis E Virus (HEV) was developed by utilizing nano-gold labeled oligonucleotide probes, silver stain enhancement and the microarray technique. The 5'-end -$NH_2$ modified oligonucleotide probes were immobilized on the surface of the chip base as the capture probe. The detection probe was made of the 3'-end -SH modified oligonucleotide probe and nano-gold colloid. The optimal concentrations of these two probes were determined. To test the detection sensitivity and specificity of this technique, a conservative fragment of the virus RNA was amplified by the RT-PCR/PCR one step amplification. The cDNA was hybridized with the capture probes and the detection probes on microarray. The detection signal was amplified by silver stain enhancement and could be identified by naked eyes. 100 fM of amplicon could be detected out on the microarray. As the results, preparation of nano-gold was improved and faster. Development time also was shortened to 2 min. Thus, considering high efficiency, low cost, good specificity and high sensitivity, this technique is alternative for the detection of HEV.

Hydrogen sulfide inhibits the growth of Escherichia coli through oxidative damage

Liu-Hui Fu,Zeng-Zheng Wei,Kang-Di Hu,Lan-Ying Hu,Yan-Hong Li,Xiao-Yan Chen,Zhuo Han,Gai-Fang Yao,Hua Zhang 한국미생물학회 2018 The journal of microbiology Vol.56 No.4

Many studies have shown that hydrogen sulfide (H2S) is both detrimental and beneficial to animals and plants, whereas its effect on bacteria is not fully understood. Here, we report that H2S, released by sodium hydrosulfide (NaHS), significantly inhibits the growth of Escherichia coli in a dose-dependent manner. Further studies have shown that H2S treatment stimulates the production of reactive oxygen species (ROS) and decreases glutathione (GSH) levels in E. coli, resulting in lipid peroxidation and DNA damage. H2S also inhibits the antioxidative enzyme activities of superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) and induces the response of the SoxRS and OxyR regulons in E. coli. Moreover, pretreatment with the antioxidant ascorbic acid (AsA) could effectively prevent H2S-induced toxicity in E. coli. Taken together, our results indicate that H2S exhibits an antibacterial effect on E. coli through oxidative damage and suggest a possible application for H2S in water and food processing.

Isolation and molecular characterization of two novel HMW-GS genes from Chinese wheat (Triticum aestivum L.) landrace Banjiemang

Hui Shao,Tian-hong Liu,Cong-Fu Ran,Li-Qun Li,Jing Yu,Xin Gao,Xue-Jun Li 한국유전학회 2015 Genes & Genomics Vol.37 No.1

High molecular weight glutenin subunits(HMW-GS) play a significant role in determining breadmakingqualities of common wheat flour. The study presentedin the paper identified two novel HMW-GS (designatedas 1Bx14* and 1By15*) at the Glu-B1 locus inChinese wheat (Triticum aestivum L.) landrace Banjiemangby SDS-PAGE. The open reading frames (ORFs) of thegenes were amplified and cloned with designed specificprimers. Both of them had sequences highly similar tothose of other HMW-GS genes, but displayed minormodification. The ORF of 1Bx14* was 2367 bp long andencoded 789 aa with 4 cysteines, which included 25nanopeptides, 65 hexapeptides and 7 tripeptides motifs. The ORF of 1By15* was 2106 bp long and encoded 702 aawith 7 cysteines, which included 23 nanopeptides and 53hexapeptides motifs. The predicted molecular weights ofthe mature proteins encoded by 1Bx14* and 1By15* geneswere 83.1 and 73.2 k Da, respectively. The presence andauthenticity of the two genes in Banjiemang were confirmedby bacterial expression and liquid chromatographyelectrosprayionization mass spectrometry (LC–ESI–MS). By phylogenetic analysis, the 1Bx14* was clustered withx-type subunits but had a high divergence with other 1Bxsubunits, whereas the 1By15* was clustered with y-typesubunits and closely related to subunit 1By15. The studyconcluded that 1Bx14* and 1By15* from Banjiemang werenovel allelic variations of HMW-GS at Glu-B1 locus,which were probably exploitable as new resources forwheat quality improvement.

Exogenous p53 Upregulated Modulator of Apoptosis (PUMA) Decreases Growth of Lung Cancer A549 Cells

Liu, Chun-Ju,Zhang, Xia-Li,Luo, Da-Ya,Zhu, Wei-Feng,Wan, Hui-Fang,Yang, Jun-Ping,Yang, Xiao-Jun,Wan, Fu-Sheng Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.2

Purpose: To investigate the influence of exogenous p53 upregulated modulator of apoptosis (PUMA) expression on cell proliferation and apoptosis in human non-small cell lung cancer A549 cells and transplanted tumor cell growth in nude mice. Materials and Methods: A549 cells were divided into the following groups: control, non-carrier (NC), PUMA (transfected with pCEP4-(HA) 2-PUMA plasmid), DDP ($10{\mu}g/mL$ cisplatin treatment) and PUMA+DDP (transfected with pCEP4-(HA)2-PUMA plasmid and $10{\mu}g/mL$ cisplatin treatment). The MTT method was used to detect the cell survival rate. Cell apoptosis rates were measured by flow cytometry, and PUMA, Bax and Bcl-2 protein expression levels were measured by Western blotting. Results: Compared to the control group, the PUMA, DDP and PUMA+DDP groups all had significantly decreased A549 cell proliferation (p<0.01), with the largest reduction in the PUMA+DDP group. Conversely, the apoptosis rates of the three groups were significantly increased (P<0.01), and the PUMA and DDP treatments were synergistic. Moreover, Bax protein levels significantly increased (p<0.01), while Bcl-2 protein levels significantly decreased (p<0.01). Finally, both the volume and the weights of transplanted tumors were significantly reduced (p<0.01), and the inhibition ratio of the PUMA+DDP group was significantly higher than in the single DDP or PUMA groups. Conclusions: Exogenous PUMA effectively inhibited lung cancer A549 cell proliferation and transplanted tumor growth by increasing Bax protein levels and reducing Bcl-2 protein levels.

Lipopolysaccharide-induced Autophagy Increases SOX2-positive Astrocytes While Decreasing Neuronal Differentiation in the Adult Hippocampus

Liu Wen-Chung,Wu Chih-Wei,Fu Mu-Hui,Tain You-Lin,Liang Chih-Kuang,Chen I-Chun,Hung Chun-Ying,Lee Yu-Chi,Wu Kay L.H. 한국뇌신경과학회 2022 Experimental Neurobiology Vol.31 No.5

Inflammation alters the neural stem cell (NSC) lineage from neuronal to astrogliogenesis. However, the underlying mechanism is elusive. Autophagy contributes to the decline in adult hippocampal neurogenesis under E. coli lipopolysaccharide (LPS) stimulation. SRY-box transcription Factor 2 (SOX2) is critical for NSC self-renewal and proliferation. In this study, we investigated the role of SOX2 in induced autophagy and hippocampal adult neurogenesis under LPS stimulation. LPS (5 ng•100 g-1•hour-1 for 7 days) was intraperitoneally infused into male Sprague–Dawley rats (8 weeks old) to induce mild systemic inflammation. Beclin 1 and autophagy protein 12 (Atg12) were significantly upregulated concurrent with decreased numbers of Ki67- and doublecortin (DCX)-positive cells in the dentate gyrus. Synchronically, the levels of phospho(p)-mTOR, the p-mTOR/mTOR ratio, p-P85s6k, and the p-P85s6k/P85s6k ratio were suppressed. In contrast, SOX2 expression was increased. The fluorescence micrographs indicated that the colocalization of Beclin 1 and SOX2 was increased in the subgranular zone (SGZ) of the dentate gyrus. Moreover, increased S100β-positive astrocytes were colocalized with SOX2 in the SGZ. Intracerebroventricular infusion of 3-methyladenine (an autophagy inhibitor) effectively prevented the increases in Beclin 1, Atg12, and SOX2. The SOX2+-Beclin 1+ and SOX2+-S100β+ cells were reduced. The levels of p-mTOR and p-P85s6k were enhanced. Most importantly, the number of DCX-positive cells was preserved. Altogether, these data suggest that LPS induced autophagy to inactivate the mTOR/P85s6k pathway, resulting in a decline in neural differentiation. SOX2 was upregulated to facilitate the NSC lineage, while the autophagy milieu could switch the SOX2-induced NSC lineage from neurogenesis to astrogliogenesis.

Improving thermal shock and ablation resistance of high thermal conductivity carbon/carbon composites by introducing carbon nanotubes

Liu Xue-Song,Fu Qian-Gang,Wang Han-Hui,Tong Ming-De,Zhang Jia-Ping,Song Qiang 한국탄소학회 2020 Carbon Letters Vol.30 No.6

In order to improve the thermal shock and ablation resistance of high thermal conductivity carbon/carbon composites, carbon nanotubes (CNTs) were introduced by electrophoretic deposition. After modifcation, the fexural strength of the composites increases by 53.0% due to the greatly strengthened interfaces. During thermal shock between 1100 °C and room temperature for 30 times, the strength continues to increase, attributed to the weakened interfaces in favor of fber and CNT pull-out. By introducing CNTs at interfaces, thermal conductivity of the composites along the fber axial direction decreases and that along the fber radial direction increases. As the thermal shock process prolongs, since the carbon structure integrity of CNT and matrix in the modifed composites is improved, the conductivity increases whatever the orientation is, until the thermal stress causes too many defects. As for the anti-ablation performance, the mass ablation rates of the CNT-modifed composites with fbers parallel to and vertical to the fame decrease by 69.6% and 43.9% respectively, and the diference in the mass ablation rate related with fber orientations becomes much less. Such performance improvement could be ascribed to the reduced oxidative damage and the enhanced interfaces.

Microstructural Evolution and Dynamic Recrystallization Behavior of the Homogenized 2195 Al–Li Alloy During Hot Deformation

Rong Fu,Yuanchun Huang,Yu Liu,Hui Li 대한금속·재료학회 2023 METALS AND MATERIALS International Vol.29 No.9

The thermal deformation behavior in homogenized 2195 Al–Li alloy was studied at different deformation temperatures(370–520 °C and strain rates (0.001–1 s−1). The flow stress decreases with decreasing strain rate or increasing temperature. The significant decrease in flow stress from 370 to 420 °C is related to the fact that movements of dislocations and (sub)grain boundaries at low temperatures are hindered by the dynamic precipitation of numerous fine secondary phases (mainlyAl2CuLi(T1) phases). Meanwhile, it was found that flow localization regions appear in the microstructure at 370 °C/1 s−1. Besides, DRX and DRV increase with decreasing lnZ (Zener–Hollomon parameter) values, particularly for lnZ values lessthan 26.32 (520 °C/0.1 s−1). The DRX mechanism was discussed for different lnZ based on the variation of misorientationangle and the characteristics of different DRXs. The DDRX is dominant when lnZ ≥ 30.70; CDRX and DDRX dominate at30.70 > lnZ > 26.32; CDRX mainly occurs at lnZ ≤ 26.32; when lnZ value decreases to 21.72, DRX grains with the characteristicsof CDRX and GDRX appear.

The Effect of Herb-Partition Moxibustion on Toll-like Receptor 4 in Rabbit Aorta during Atherosclerosis

Zeng-Hui Yue,Xin-Qun He,Xiao-Rong Chang,Jian-Ling Yuan,Bao-Sheng Yu,Mi Liu,Ling Fu,Liang Zhang,Li-Chao Shang 사단법인약침학회 2012 Journal of Acupuncture & Meridian Studies Vol.5 No.2

Objective: To explore the mechanism of Toll-like receptor (TLR4) inhibition in the delay of formation of atherosclerosis by herb-partition moxibustion. Method: Seventy-five rabbits were randomly assigned to one of five groups: blank, atherosclerosis (AS) model, direct moxibustion, herb-partition moxibustion, and drug treatment. With the exception of the blank group, all rabbits were given a high-fat diet in addition to immunologic injury to create the AS model. The experiments were carried out for 16 weeks, at which time the aorta was removed from each rabbit. Immunohistochemical methods were used to detect the gray level of the aortic TLR4 to observe the immunologic competence of its antigens. Fluorescent quantitative polymerase chain reaction was used to detect the expression of TLR4 messenger RNA (mRNA) in the aorta. Results: The gray-scale value of TLR4 and the TLR4 mRNA expression significantly decreased (p < 0.05) in the direct moxibustion, herb-partition moxibustion, and drug treatment groups. Furthermore, the effects of the herb-partition moxibustion and drug treatment were superior to those of the direct moxibustion. Conclusion: Herb-partition moxibustion inhibits aortic TLR4 activity and mRNA expression,showing that herb-partition moxibustion delays the formation of atherosclerosis through the inhibition of TLR4 expression.

Hydroxydibenzoylmethane induces apoptosis through repressing ornithine decarboxylase in human promyelocytic leukemia HL-60 cells

Ming-Fu Wang,Guang-Yaw Liu,Ya-Fan Liao,Ying-Cheng Hung,Chih-Li Lin,Tzyh-Chyuan Hour,Ko-Huang Lue,Hui-Chih Hung 생화학분자생물학회 2011 Experimental and molecular medicine Vol.43 No.4

Ornithine decarboxylase (ODC) is the rate-limiting enzyme in polyamine biosynthesis and a target for chemoprevention. Hydroxydibenzoylmethane (HDB),a derivative of dibenzoylmethane of licorice, is a promising chemopreventive agent. In this paper, we investigated whether HDB would inhibit the ODC pathway to enhance apoptosis in human promyelocytic leukemia HL-60 cells. We found ODC enzyme activity was reduced during HDB treatment. Overexpression of ODC in HL-60 parental cells could reduce HDB-induced apoptosis, which leads to loss of mitochondrial membrane potential (Δψ m), through lessening intracellular ROS. Furthermore, ODC overexpression protected cytochrome c release and the activation of caspase-3 following HDB treatment. The results demonstrated HDB-induced apoptosis was through a mechanism of down-regulation of ODC and occurred along a ROS-dependent mitochondria-mediated pathway.