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Porphyromonas endodontalis의 침투에 따른 혈관 내피세포의 유전자 발현
공희정,최경규,박상혁,이진용,최기운 대한치과보존학회 2009 Restorative Dentistry & Endodontics Vol.34 No.6
During the last two decades, there has been an increasing interest in the impact of oral health on atherosclerosis and subsequent cardiovascular disease (CVD). To date, some periodontal pathogens including Porphyromonas gingivalis (P. gingivalis) have been reported to be relevant to CVD. Porphyromonas endodontalis (P. endodontalis), which shares approximately 87% sequence homology with P. gingivalis, is mostly found within infected root canals. However, recent studies reveal that this pathogen also resides in the dental plaque or periodontal pocket in patients with periodontitis. It has been shown that P. endodontalis invades human coronary artery endothelial cells (HCAEC) and coronary artery smooth muscle cells (CASMC). To evaluate whether P. endodontalis can participate in the progression of atherosclerosis and CVD, we examined the changes in transcriptional gene expression profiles of HCAEC responding to invaion by P. endodontalis in this study. The following results were obtained. 1. Porphyromonas endodontalis was invasive of HCAEC. 2. According to the microarray analysis, there were 625 genes upregulated more than two-folds, while there were 154 genes downregulated by half. 3. Upregulated genes were relevant to inflammatory cytokines, apoptosis, coagulation and immune response. Enhanced expression of MMP-1 was also noticeable. 4. The transcription profiles of the 10 selected genes examined by real-time PCR agreed well with those observed in the microarray analysis. Thus, these results show that P. endodontalis presents the potential to trigger and augment atherosclerosis leading to CVD. 본 연구에서는 Porphyromonas endodontalis와 죽상경화증 및 심혈관질환과의 관계를 알아보기 위해, P. endodontalis가 사람의 관상동맥 내피세포에 침투했을 때 나타나는 유전자 발현의 변화를 microarray와 real-time PCR로 측정하였고, 발현이 증가된 유전자 중에서 죽상경화증과 연관된 유전자들이 관련 KEGG pathway 상에서 유의성 있는 영향을 미치는지를 분석하여 다음과 같은 결과를 얻었다. 1. Porphyromonas endodontalis는 사람의 관상동맥 내피세포에 침투하였다. 2. Microarray 분석결과, 대조군보다 발현이 2배 이상 증가된 유전자는 625개였고, 1/2이하로 감소된 유전자는 154개였다. 3. 발현이 2배 이상 증가된 유전자 중에는 염증성 cytokine 및 chemokine, 세포자멸사, 혈액응고와 면역반응 관련 유전자들이 포함되었다. 4. Microarray 분석결과를 확인하기 위해 발현이 2배 이상 증가된 유전자 중에서 10개의 유전자를 선택하여 real-time PCR을 시행하였고, 그 결과 microarray에서와 마찬가지로 발현 정도가 대조군보다 모두 높게 나타났다. 따라서 P. endodontalis가 사람의 관상동맥 내피세포에 만성적으로 작용했을 때, 심혈관질환에서 중요한 부분을 차지하는 죽상경화증을 유발하는 위험 요소 중의 하나로 작용할 수 있을 것으로 판단된다. 이와 관련된 자세한 기전을 이해하기 위해서는 앞으로 더 많은 연구가 필요할 것으로 보인다.
Thioacetamide에 의한 BALB/c 마우스 간의 시간별 약물대사효소 억제 양상 : A Time-Course Study
이정운,고우석,김갑호,배연경,하현정,한상섭,천영진,정태천 영남대학교 약품개발연구소 2001 영남대학교 약품개발연구소 연구업적집 Vol.11 No.-
Thioacetamide is a potent hepatotoxicant which requires metabolic activation by cytochrome P450s (P450s) for toxicity. In the present study, the elevation kinetic of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities by thioacetamide treatment was investigated in male BALB/c mice. Inaddition, the inhibitory effects of thioacetamide on liver microsomal P450 enzymes were further investigated. Thioacetamide at 100 mg/kg/ was treated intraperitoneally for 6, 12, 24, 36, 48 and 72 hr. The blood was collected at the designated time for assaying the serum enzyme activities. To determine the P450 isozyme-specific activities. ethoxyresorufin O-deethylase (EROD), methoxyresorufin O-demethylase (MROD), and benzyloxyresorufin O-debenzylase (BROD) activities were determined for P450 1A1, 1A2 and 2B1, respectively, in liver microsomal fractions. The activities of ALT and AST were started to be elevated 6 hr after thioacetamide treatment andreached the maximun at 36 hr after the treatment. The elevated activities were dramatically recovered at 72 hr. The microscopic exmination of the liver specimen also showed a similar profile of hepatotoxicity. All P450-associated enzyme activities were time-dependently inhibited by the treatiment with thioacetamide. The maximum inhibition of P450 enzymes was observed 36 hr after the treatment. Because the inhibition of P450 enzymes by thioacetamide was time-dependent, our present results suggest that thioacetamide might inhibit P450 enzymes in mechanism-based inactivation.
Kyoung-Woon Joung,Jin-Ho Rhim,Ji-Hyun Chin,Wook Jong Kim,Dae Kee Choi,Eun Ho Lee,Kyung-Don Hahm,Ji Yeon Sim,In-Cheol Choi 대한마취통증의학회 2013 Korean Journal of Anesthesiology Vol.65 No.5
Background: Several studies have shown in animal models that remote ischemic preconditioning (rIPC) has a neuroprotective effect. However, a randomized controlled trial in human subjects to investigate the neuroprotective effect of rIPC after cardiac surgery has not yet been reported. Therefore, we performed this pilot study to determine whether rIPC reduced the occurrence of postoperative cognitive dysfunction in patients who underwent off-pump coronary artery bypass graft (OPCAB) surgery. Methods: Seventy patients who underwent OPCAB surgery were assigned to either the control or the rIPC group using a computer-generated randomization table. The application of rIPC consisted of four cycles of 5 min ischemia and 5 min reperfusion on an upper limb using a blood pressure cuff inflating 200 mmHg before coronary artery anastomosis. The cognitive function tests were performed one day before surgery and again on postoperative day 7. We defined postoperative cognitive dysfunction as decreased postoperative test values more than 20% of the baseline values in more than two of the six cognitive function tests that were performed. Results: In the cognitive function tests, there were no significant differences in the results obtained during the preoperative and postoperative periods for all tests and there were no mean differences observed in the preoperative and postoperative scores. The incidences of postoperative cognitive dysfunction in the control and rIPC groups were 28.6% (10 patients) and 31.4% (11 patients), respectively. Conclusions: rIPC did not reduce the incidence of postoperative cognitive dysfunction after OPCAB surgery during the immediate postoperative period.
독소루비신 전달을 위한 메톡시 폴리에틸렌글라이콜이 그라프트된 카르복실메틸 키토산의 제조 및 효능
이경재 ( Kyoung Jae Lee ),남정표 ( Joung Pyo Nam ),박성철 ( Seong Cheol Park ),박영훈 ( Yung Hoon Park ),나재운 ( Jae Woon Nah ),장미경 ( Mi Kyeong Jang ) 한국키틴키토산학회 2012 한국키틴키토산학회지 Vol.17 No.3
A drug delivery system based on micelles or nanoparticles has attended due to the potential for long-circulation times, prevention of undesirable side effects, sustained release, and enhancing solubility of drug, Although doxorubicin (DOX), used in this study, is an anticancer drug in treatment of human malignancies, it has substantial risk of cardiomyopathy and lifethreatening heart failure. Therefore, we prepared methoxy poly (ethylene glycol) (PEG) and DOX-grafted O-carboxymethyl chitosan (OCMCh) used to reduce cytotoxicity and enhance treatment efficacy due to the enhanced permeability and retention effect. This self-aggregated nanoparticle was characterized by fourier transform infrared (FT-IR) and proton nuclear magnetic resonance (H NMR), dynamic light scattering (DSC), and transmission electron microscope (TEM), The results showed that the nanoparticles were spherical and their size were less than 400 nm. Its anti-tumor activity and cytotoxicity of DOX were investigated agaisnt MCF-7 and HEK293 cells in vitro, respectively, resulted in a significant increasing of anti-tumor activity upon Dox-grafting amounts and a reduced cytotoxicity compared to free Dox. It can be concluded that the OCMPEGDOX was a potential drug carrier.
茶種子 發芽에 關한 硏究 : 採種時期와 溫度 處理가 茶種子의 發芽에 미치는 影響
趙慶淑(Kyoung Sook Cho),房極必(Guk Pil Bang),金正云(Joung Woon Kim),金冑禧(Ju Hee Kim),崔炯局(Hyoung Koog Choi) 한국차학회 1995 한국차학회지 Vol.1 No.1
This experiment was conducted to find out tea seed germination by six tea seed picking periods from September 5 to November 25 at intervals of twenty days. Low temperature treatment did cold moist prechilling storage for 30 days at 0, 5, 10˚C and then germinated at 20˚C. The normal temperature germinated respectively at 15, 20, 25 and 30˚C. Tea seed optimum germination temperature was between 20˚C~25˚C. Cold moist prechilling effect(Storage 30 days at 5, 10˚C) was not only shorter germination period but germination percentage was increased too. In case of doing cold moist prechilling, during the period of September 25 to November 5 between ±20 days from October 15 of optimum picking period could expect high rate of the germination more than 90%.
배출허용기준 강화에 따른 차속별 경유 중형트럭의 유해대기오염물질 배출특성
홍희경 ( Hee Kyoung Hong ),정성운 ( Sung Woon Jung ),손지환 ( Ji Hwan Son ),문태영 ( Tae Young Moon ),이상은 ( Sang Eun Lee ),문선희 ( Sun Hee Moon ),윤현진 ( Hyun Jin Yoon ),김정수 ( Jeong Soo Kim ),김정화 ( Joung Hwa Kim ) 한국분무공학회 2015 한국액체미립화학회지 Vol.20 No.2
This study was designed to investigate the emission characteristics of unregulated pollutants (Aldehyde, VOCs, PAHs) as well as regulated pollutants (CO, HC, NOx and PM) from diesel medium-duty trucks. The emission characteristics of unreg- ulated and regulated pollutants were assessed based on regulation standards (EURO 4 and EURO 5) and intake weight (2.5 ton and 5 ton). The results show that unregulated and regulated pollutants remained almost unchanged at higher speeds but decreased at below 23.5 km/h. Reduction in unregulated and regulated pollutants was noticeable in vehicles of recent regu- lation standards and light intake weight. The analysis of aldehyde using UPLC showed that formaldehyde and acetaldehyde of aldehyde were most dominant. The GC/MS analysis showed that benzene, toluene, ethylbenzene and xylene of VOCs was over 80% followed by toluene, xylene, ethylbenzene and benzene. In addition, the analysis of PAHs using GC/TOF-MS indi- cated that bi- and tricyclic aromatic ring of aromatic compounds was 73% and 53% at 2.5 ton and 5 ton vehicles, respec- tively. The results of this study will be contributed to establish HAPs inventory.
연료첨가제 주입에 따른 승용차의 규제물질 배출특성 분석
정성운 ( Sung Woon Jung ),손지환 ( Ji Hwan Son ),홍희경 ( Hee Kyoung Hong ),성기재 ( Ki Jae Sung ),김정수 ( Jeong Soo Kim ),김정화 ( Joung Hwa Kim ) 한국분무공학회 2015 한국액체미립화학회지 Vol.20 No.4
This paper was designed to investigate emission characteristics of regulated pollutants (CO, HC, NOx and PM) from 134 diesel and gasoline passenger cars based on emission standards according to fuel additives. The experiments using chassis dynamometer were conducted under NEDC and CVS-75 modes. Comparison for fuel additive management and test between Korea, USA, EU and Japan, Korea was more strict than others. The fuel additives of this study was satisfied within fuel manufacturing standards. For with/without fuel additives according to diesel emission standards, NOx of EURO 4 and EURO 5 showed a relatively similar tendency. In the case of PM reduction rate, EURO 5 was over 20% increased than EURO 4.In the case of standard deviation/average ratio for gasoline vehicles, variation interval was big for LEV 23.3~58% and ULEV 31.6~56.4%. Following the imposition of stricter regulations (EURO 5 and ULEV), difference rate for standard deviation was big. Especially, in the case of diesel vehicles, difference rate for NOx 68% and PM 48% was most big. The results of present study will be of assistance in completing the legislative process and will provide basic data to set up emission standards for fuel additives in Korea.