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신현진,김철중,신광순 충남대학교 수의과대학 동물의과학연구소 1995 動物醫科學硏究誌 Vol.3 No.-
C형 간염 바이러스(HCV)의 구조 유존자중 core 유전자를 fusion protein vector인 pGEX-2T vector에 cloning하여 core단백질을 발현시켜서 항원성을 증가시키고자 하였다. HCV의 core gene을 PCR (polymerase chain reaction) 기법을 이용하여 증폭한 후, 약 0.57kb 정도의 증폭된 core gene을 TA cloning vector에 cloning 한 후, restriction exzyme BamH I으로 잘라, gel elution method로 elution하여 pGEX-2T vector의 BamH I site에 cloning 시켰다. Cloning된 plasmid를 E coli cell인 JM105에 transformation시킨 후, IPTG에의해 protein induction 하였다. pGEX-2T에서 발현된 HCV core 단백질은 SDSPAGE및 Western-blotting 분석 결과, 양성 C형 간염 환자의 serum및 monoclonal antibody와 특이적으로 반응하였다.
辛炯泰,韓泳根,李光淳 成均館大學校 1982 論文集 Vol.31 No.-
A factorial experiment (6x5) involving 6 kinds of crop residues (barley straw, corn stover, rice straw, rye straw, soybean straw, wheat straw), 5 levels of sodium hydroxide (0,3,6,9,12%) was conducted with four Korean native male goats using a practical dairy cattle ration. Crop residues digestion was measured in situ with nylon bags containing test material in the rumen of the fistulated goats. Bags of 9×15㎠ made of 100-mesh nylon cloth were filled with one of ground crop residues to give 3-5g dry matter. These bags were put into the ventral sac of the rumen and taken out at the end of 72 hours. They were washed in cold water, dried at 105℃ for 8 hours and weighed. The straw dry matter remaining was calculated by difference and analyzed for organic matter. The results which were obtained with this experiment were as follows: 1. The nylon bag dry matter digestibility (NBDMD, %) and nylon bag organic matter digestibility (MBOMD, %) of NaOH-treated crop residues were highly significant different (P<0.01) in different levels of NaOH and kinds of crop residues. 2. The relationship between cell-wall constituents (Y, %) of crop residues and level of NaOH (X, %) was: Y=-1.63X+77.06 (r=0.99^**) 3. The optimum level of NaOH for improving NBDMD (%) from crop residues, on the basis of Shin et al (1981), was 0% NaOH for soybean straw, 3% NaOH for barley straw and wheat straw, 6% NaOH for corn stover and rice straw, and 12% NaOH for rye straw.
조태순,이선미,김낙두,허인회,안형수,권광일,박석기,심상호,신대희,박대규 성균관대학교 약학연구소 1999 成均藥硏論文集 Vol.11 No.-
Abstract-In order to investigate the phamacological properties of New Wonbang Woohwangchungsimwon Pill (NSCH), effects of Wonbang Woohwangchungsimwon Pill (SCH) and NSCH were compared using various experimental models. In rat aorta, NSCH and SCH made the relaxation of blood vessels in maximum contractile response to phenylephrine (10^-6 M) regardless to endothelium containing or denuded rings of the rat aorta. Furthermore, the presence of the inhibitors of NO synthase and guanylate cyclase did not aftect significantly the relaxing effects of NSCH and SCH, NSCH and SCH inhibited the vascular contractions induced by acetylcholine, prostaglandin endoperoxide or peroxide in a dose-dependent manner. In conscious spontaneously hypertensive rats (SHRs). NSCH and SCH decreased significantly heart rate. These, at high doses, had a negative inotropic effect that was a decrease of left ventricular developed pressure and (-dp/dt)/(+dp/dt) in the isolated perfused rat hearts, and also decreased the contractile force and heart rate in the isolated rat right atria. In guinea-pig papillary muscle, these had no effects on parameters of action potential such as action potential amplitude (APA). V_max and resting membrane potential (RMP) at low doses, whereas inhibited the cardiac contractility at high doses. Furthermore, these had a significant inhibitory effects on palpitation of the heart in normotensive rats and SHRs. These results suggest that NSCH and SCH have weak cardiovascular effects, and that there is no significant differences between cardiovascular effects of two preparations.
조태순,이선미,김낙두,허인회,안형수,권광일,박석기,심상호,신대희,박대규 충남대학교 약학대학 의약품개발연구소 1999 藥學論文集 Vol.15 No.-
In order to investigate the pharmacological properties of New Wonbang Woohwangchungsimwon Liquid (NSCL), effects of Wonbang Woohwangchungsimwon Liquid (SCL) and NSCL were compared. In isolated rat aorta, NSCL and SCL showed the relaxation of blood vessels in maximum contractile response to phenylephrine (10^-6M) regardless to intact endothelium or denuded rings of the rat aorta. Furthermore, the presences of the inhibitor of NO synthase and guanylate cyclase did not affect the relaxing effect of NSCL and SCL. NSCL and SCL inhibited the vascular contractions induced by acetylcholine, prostaglandin endoperoxide or peroxide in a dose-dependent manner. In conscious spontaneously hypertensive rats (SHRs), NSCL and SCL significantly decreased heart rate. NSCL and SCL, at high doses, had a negative inotropic effect that was a decrease of left ventricular developed pressure and (-dp/dt)/(+dp/dt) in the isolated perfused rat hearts, and also decreased the contractile force and heart rate in the isolated rat right atria. In excised guinea-pig papillary muscle, NSCL and SCL had no effects on parameters of action potential such as resting membrane potential, action potential amplitude, APD_90 and V_max at low doses, whereas inhibited the cardiac contractility at high doses. These results suggested that NSCL and SCL have weak cardiovascular effects with relaxation of blood vessels and decrease of heart rate, and that this effect is no significant differences between cardiovascular effects of two preparations.
조태순,이선미,김낙두,허인회,안형수,권광일,박석기,심상호,신대희,박대규 충남대학교 약학대학 의약품개발연구소 1997 藥學論文集 Vol.13 No.-
In order to investigate the pharmacological properties of New Woohwangchungsimwon Liquid (NCL), efects of Woohwangchungsimwon Liquid (CL) and NCL were compared. In isolated rat aorta, NCL and CL showed the relaxation of blood vessels in maximum contractile response to phenylephrine (10^6M) without regard to intact endothelium or denuded rings of the rat aorta. Furthemore, the presences of the inhibitor of NO synthase and guanylate cyclase did not affect the relaxation of NCL and CL. NCL and CL inhibited the vascular contractions induced by acetylcholine, prostaglandin endoperoxide or peroxide in a dosedependent manner. In conscious spontaneously hypertensive rats (SHRs), NCL and CL significantly decreased heart rate. NCL and CL, at high doses, had a negative inotropic effect that was a decrease of LVDP and (-dp/dt)/(+dp/dt) in the isolated perfused rat hearts, and also decreased the contractile force and heart rate in the isolated rat right atria. In excised guinea-pig papillary muscle, NCL and CL had no efects on parameters of action potential at low doses, whereas inhibited the cardiac contractility at high doses. These results suggested that NCL and CL have weak cardiovascular effects with relaxation of blood vessels and decrease of heart rate, and that this effect is no significant differences between two preparations.