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You-Young Jin,Kyung Ju Ku,Jiyong Park,Jonghyun Park,Myongsoo Chung,Kisung Kwon,Kyungsook Chung,Misun Won,Kyung Bin Song 한국식품과학회 2008 Food Science and Biotechnology Vol.17 No.4
Enterobacter sakazakii, Salmonella typhimurium, and Bacillus cereus were evaluated on inoculated infant formula by gamma irradiation treatment as a method to provide microbial safety. The infant formula inoculated with the major pathogenic bacteria was treated at irradiation dose of 0, 3, 5, and 10 kGy, respectively. After treatment, the samples were individually packaged and stored at 20℃. Microbiological data during storage represented that the populations of E. sakazakii, S. typhimurium, and B. cereus were reduced with the increase of irradiation dose by 4 to 5 log reductions. In particular, E. sakazakii, S. typhimurium, and B. cereus were eliminated at 10, 5, and 3 kGy, respectively. E. sakazakii was the most radiation-resistant, while B. cereus was the least. Our results represent that gamma irradiation below 10 kGy should eliminate the growth of the major pathogenic bacteria in infant formula during storage.
Jin, Jeong-Un,Lee, Dong-Hoon,Nam, Ki-Ho,Yu, Jaesang,Kim, Young-Kwan,Goh, Munju,Kim, Seo Gyun,Lee, Heon Sang,Ku, Bon-Cheol,You, Nam-Ho Elsevier 2019 APPLIED SURFACE SCIENCE - Vol.464 No.-
<P><B>Abstract</B></P> <P>We synthesized methylpiperidine-functionalized graphene oxide (MP-GO) by introducing 4-amino-1-methylpiperidine into reactive epoxy and/or carboxylic acid groups on pristine GO. Then, we applied the MP-GO as a curing catalyst for polyimide (PI) nanocomposites. The MP-GO was found to be an effective base-catalysts for the thermal conversion of polyamic acid (PAA) precursor to PI. Interestingly, when 3 wt% of MP-GO was added to the PI matrix, the complete imidization of nanocomposites was achieved at a temperature lower than 200 °C. In addition, the PI/MP-GO nanocomposite films exhibited reinforcement of the oxygen barrier properties which were even better than those of pristine PI, due to the excellent dispersion state of MP-GO and the favorable non-covalent interaction between MP-GO and the PI matrix. Comparison to pristine PI, the oxygen permeability of nanocomposite films that contained only 1 wt% of MP-GO loading was significantly decreased, by about 80%. Furthermore, all the PI/MP-GO nanocomposites exhibited high thermal stability.</P> <P><B>Highlights</B></P> <P> <UL> <LI> MP-GO was prepared by introducing 4-amino-1-methypiperidine. </LI> <LI> The MP-GO can act as a curing catalyst for polyimide nanocomposites. </LI> <LI> The oxygen barrier property of PI/MP-GO 1 wt% was 80% better than pristine PI. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Effect of Electron Beam Irradiation on Microbial Growth and Qualities in Astragalus membranaceus
Jin, You-Young,Shin, Hee-Young,Ku, Kyoing-Ju,Song, Kyung-Bin The Korean Society for Applied Biological Chemistr 2006 Journal of Applied Biological Chemistry (J. Appl. Vol.49 No.4
Electron beam irradiation was applied to examine the microbial growth and qualities of vacuum-packaged Astragalus membranaceus, a Korean medicinal herb. Samples were irradiated at dose of 2, 4, 8, 12, and 16 kGy, respectively. Microbiological data on A. membranaceus showed that populations of total bacteria, yeast and mold, total coliforms were significantly reduced with increase of irradiation dose. Populations of microorganisms in A. membranaceus were decreased by 2-3 log cycles at 8 kGy irradiation. Color measurements showed that electron beam treatment caused negligible changes in Hunter color L, a, and b values of A. membranaceus. Sensory evaluations showed that there were no significant changes among the samples. These results suggest that electron beam irradiated A. membranaceus have better microbial safety and qualities, compared with the non-irradiated control.
Amperometric Tyrosinase Biosensor Based on Carbon Nanotube–Titania–Nafion Composite Film
Lee, You-Jin,Lyu, Young-Ku,Choi, Han ,Nim,Lee, Won-Yong WILEY-VCH Verlag 2007 Electroanalysis Vol.19 No.10
<P>A highly sensitive and stable amperometric tyrosinase biosensor has been developed based on multiwalled carbon nanotube (MWCNT) dispersed in mesoporous composite films of sol–gel-derived titania and perfluorosulfonated ionomer (Nafion). Tyrosinase was immobilized within a thin film of MWCNT–titania–Nafion composite film coated on a glassy carbon electrode. Phenolic compounds were determined by the direct reduction of biocatalytically-liberated quinone species at −100 mV versus Ag/AgCl (3 M NaCl) without a mediator. The present tyrosinase biosensor showed good analytical performances in terms of response time, sensitivity, and stability compared to those obtained with other biosensors based on different sol–gel matrices. Due to the large pore size of the MWCNT–titania–Nafion composite, the present biosensor showed remarkably fast response time with less than 3 s. The present biosensor responds linearly to phenol from 1.0×10<SUP>−7</SUP> M to 5.0×10<SUP>−5</SUP> M with an excellent sensitivity of 417 mA/M and a detection limit of 9.5×10<SUP>−8</SUP> M (S/N=3). The enzyme electrode retained 89% of its initial activity after 2 weeks of storage in 50 mM phosphate buffer at pH 7.0.</P>