성선자극호르몬유리호르몬 길항제인 Antide가 수컷 생쥐의 유해감각예민도에 미치는 영향

박정현,박제민,김명정,이국희,최상헌,장세헌 대한생물치료정신의학회 2001 생물치료정신의학 Vol.7 No.1

To investigate testosterone effect on nociception, tail flick latency(TFL, sec, 50±1℃) was measured before and after administration of antide(a gonadotropin releasing hormone antagonist) in male mice(Institute of Cancer Research, age of 7-8 weeks, weight 30.5-37.5gm). Testosterone replacement effect on TFL was also examined in antide pre-treated male mice. Experiment 1:30 male mice were randomized into 3 groups(N=10 each). Antide 100㎍/kg, 300㎍/kg or same volume(5.7ml/kg) of 0.2% bovine serum albumin was administered intraperitoneally. TFL was measured before and at 3,6, 9, 12 and 24 hours after injection. Experiment 2 : 40 novice male mice were randomized into 2 groups(N=20 each) and both groups were pre-treated with antide 300㎍/kg i.p.6 hours after antide injection, testosterone 1mg/kg or same volume(5.7ml/kg) of sesame oil was administered i.p. TFL was measured before and 6 hours after antide pre-treatment and 30 min after testosterone/vehicle injection. 1) TFL was significantly shortened by antide in dose dependent manner, In control group, baseline TFL(mean±SD) and those measured at 3,6,9,12 and 24 hours after injection were 4.8±0.7, 5.6±0.7, 5.3±0.6, 5.4±0.6 and 4.7±0.6 sec, respectively. In antide 100㎍/kg group, TFLs were 5.2±0.9, 5.1 ±1.1, 4.2±0.9, 4.4±0.8, 5.1±0.8, and 4.7±0.8 sec, respectively. Compared with baseline, significant hyperalgesia appeared 6 and 9 hours after treatment. TFL measured at 6 hours after treatment was significantly shorter than control. In antide 300㎍/kg group, TFLs were 5.6±0.4, 4.5±0.9, 4.3±0.6, 3.9±1.0, 4.6±0.8 and 4.7±0.9 sec. Significant hyperalgesia appeared 3 hours after treatment and continued thereafter. TFLs measured at 3, 6 and 9 hours after treatment were significantly shorter than control, and TFL at 9 hours was significantly shorter than antide 100㎍/kg group. 2) Hyperalgesia induced by antide pre-treatment was recovered by testosterone replacement. In control group. TFL was shortened by antide pre-treartment(baseline : 6.1±1.0 sec ,6 hr after antide : 4.7±1.0 sec) and there was no significant change after vehicle injection(4.6±0.8 sec). In testosterone group, shortening of TFL induced by antide pre-treatment(baseline: 6.2±0.8 sec; 6 hr after antide: 4.6±0.9 sec) disappeared after testosteronen replacement(0.6±0.8sec). From these results, it is suggested that testosterone has a role of maintaining baseline antinociception and acute decrease in testosterone level results in hyperalgesia.

The Leading CDMO for Microbial-based Biopharmaceuticals

Kook-Heon SEO 한국생물공학회 2023 한국생물공학회 학술대회 Vol.2023 No.4

Platelet-Activating Factor Induces Up-regulation of Antiapoptotic Factors in a Melanoma Cell Line through Nuclear Factor-κB Activation

Heon Seo, Kook,Ko, Hyun-Mi,Kim, Han-A,Choi, Jung-Hwa,Jun Park, Sung,Kim, Kyoung-Jin,Lee, Hern-Ku,Im, Suhn-Young American Association for Cancer Research 2006 Cancer Research Vol.66 No.9

<P>In this study, we investigated the influence of platelet-activating factor (PAF) on the induction of apoptosis-regulating factors in B16F10 melanoma cells. PAF increased the expression of mRNA and the protein synthesis of antiapoptotic factors, such as Bcl-2 and Bcl-xL, but did not increase the expression of the proapoptotic factor, Bax. A selective nuclear factor-kappaB (NF-kappaB) inhibitor, parthenolide, inhibited the effects of PAF. Furthermore, PAF inhibited etoposide-induced increases in caspase-3, caspase-8, and caspase-9 activities, as well as cell death. p50/p65 heterodimer increased the mRNA expression of Bcl-2 and Bcl-xL and decreased etoposide-induced caspase activities and cell death. In an in vivo model in which Matrigel was injected s.c., PAF augmented the growth of B16F10 cells and attenuated etoposide-induced inhibition of B16F10 cells growth. These data indicate that PAF induces up-regulation of antiapoptotic factors in a NF-kappaB-dependent manner in a melanoma cell line, therefore suggesting that PAF may diminish the cytotoxic effect of chemotherapeutic agents.</P>

Infectious in vivo Transcripts from a Full-length Clone of Soybean mosaic virus Strain G5H

Seo, Jang-Kyun,Lee, Hyeok-Geun,Choi, Hong-Soo,Lee, Su-Heon,Kim, Kook-Hyung The Korean Society of Plant Pathology 2009 Plant Pathology Journal Vol.25 No.1

An infectious full-length clone of Soybean mosaic virus (SMV) strain G5H was constructed under the control of the cauliflower mosaic virus 35S promoter. The cloned SMV G5H established infections upon simple rub-inoculation of soybean leaves with intact plasmid DNA. We demonstrated that this SMV G5H infectious DNA clone caused typical characteristic symptoms and virulence of SMV strain G5H in twelve tested soybean cultivars. Soybean cultivars Lee74, Somyungkong and Sowonkong developed systemic mosaic symptom while Kwanggyo, Taekwangkong, Hwangkeumkong and Geumjeongkong-l showed systemic necrosis. In contrast, Geumjeongkong-2, Jinpumkong-2, L29, V94-5152 and Ogden showed resistant response against SMV-G5H infection. We also determined full-length sequence of cloned SMV-G5H. The phyogenetic analyses reveal that SMV-G5H is most closely related to SMV-G5, and support that SMV-G5H might be derived from SMV-G5 by recombination rather than mutation.

응급실에서의 중증도 분류체계의 개발

서강석,이정헌,김종근,감신,박정배,윤영국 경북대학교 의학연구소 2000 경북대학교병원의학연구소논문집 Vol.4 No.1

Background: To examine whether nonemergency patients can be identified and be safely triaged out of'the emergency department. Methods: Adult patients(16 years or older) who visited to the emergence department were prospectively studied from July 1 to August 31, 1997. Authors developed a new triage composed of vital signs, mental status, chief complaints, etiology, and a mode of transfer. Chi-square test and a discriminant analysis were applied for statistical analysis. Results: In a discriminant analysis, mode of transfer, chief complaint, age, heart late, mental status, etiology, and body temperature were significant variables in orders. In a canonical discriminant value at group mean, a nonemergent valse is 0.450 and a emergent value -0.219. Conclusions: In simply applicable triage method, made of transfer and chief complaints are more important than vital sign and mental status. This method is useful in disaster situation and non-medical personnel may use this triage method easily. But some patients are not triaged by this triage method, thus more studies and discussions are necessary.

Interaction Study of Soybean mosaic virus Proteins with Soybean Proteins using the Yeast-Two Hybrid System

Seo, Jang-Kyun,Hwang, Sung-Hyun,Kang, Sung-Hwan,Choi, Hong-Soo,Lee, Su-Heon,Sohn, Seong-Han,Kim, Kook-Hyung The Korean Society of Plant Pathology 2007 Plant Pathology Journal Vol.23 No.4

Interactions between viral proteins and host proteins are essential for virus replication. Especially, translation of viral genes completely depends on the host machinery. In potyviruses, interactions of genome-linked viral protein (VPg) with host translation factors including eIF4E, eIF(iso)4E, and poly(A)-binding protein (PABP) has previously been characterized. In this study, we investigated interactions between Soybean mosaic virus (SMV) viral proteins and host translation factors by yeast two-hybrid system. SMV VPg interacted with eIF4E, eIF(iso)4E, and PABP in yeast two-hybrid system, while SMV helper component proteinase (HC-pro) interacted with neither of those proteins. The interaction between SMV NIb and PABP was also detected. These results are consistent with those reported previously in other potyviruses. Interestingly, we found reproducible and specific interactions between SMV coat protein (CP) and PABP. Deletion analysis showed that the region of CP comprising amino acids 116 to 206 and the region of PABP comprising amino acids 520 to 580 are involved in CP/PABP interactions. Soybean library screening with SMV NIb by yeast two-hybrid assay also identified several soybean proteins including chlorophyll a/b binding preprotein, photo-system I-N subunit, ribulose 1,5-biphosphate carboxylase, ST-LSI protein, translation initiation factor 1, TIR-NBS type R protein, RNA binding protein, ubiquitin, and LRR protein kinase. Altogether, these results suggest that potyviral replicase may comprise a multi-protein complex with PABP, CP, and other host factors.

응급실 내원 환자의 만족도 평가 및 관련 요인에 대한 연구

서강석,감신,박정배,이정헌,김종근,윤영국,곽경숙,이원기,우석정 大韓應急醫學會 1998 대한응급의학회지 Vol.9 No.4

Background: To examine the influencing factors on patients' satisfaction in the emergency department(ED) for quality assurance. Methods: Patients who visited to the ED were prospectively investigated from November 1 to December 31, 1997. Authors developed questionnaire to investigate influencing factors on patients' satisfaction. A Chi-square test and LISREL 7.0 were applied for statistical analysis. Results: Patients' satisfaction was significantly related to physical environment variables, accessibility variables, kindness of hospital personnel variables, and patient's trust for doctors variables. In path analysis, willingness for revisit was influenced by patients' satisfation, accessibility, physical environment, patients' trust for doctors in order, and willingness for recommendation was influenced by accessibility, patients' satisfaction, and kindness of hospital personnel in order. Conclusions: The influencing factors on patients' satisfaction are physical environment, accessibility, kindness of hospital personnel, and patient's trust for doctors. Willingness for revisit and willingness for recommendation are influenced by patients' satisfation. In spite of some limitations, the results of this study can be used as a baseline information for exploring the influencing factors on patients' satisfaction. Further comprehensive research efforts should be made on the study of patients' satisfactoin in the ED.

당뇨병성 케톤산증에 대한 임상적 고찰

서강석,이정헌,박정배,김종근,윤영국 대한응급의학회 1998 대한응급의학회지 Vol.9 No.1

Background: Diabetic ketoacidosis(DKA) is serious acute metabolic complication and the most important cause of high morbidity and mortality of diabetes. The object of this study is to examine the clinical characteristics of patients with DKA who had a prior history of diabetes or not. Method: Authers reviewed retrospectively the medical records of 49 cases adimitted to Kyungpook National University Hospital from January 1991 to June 1997 with a diagnosis of DKA and classified cases as type I, type II and newly diagnosed diabetics according to prior history of diabetes. Results: 1. Of 49 cases of DKA, 24(49%) were classified as type I, 17(35%) as type II from data available in the medical records, and 8(16%) had DKA as the initial manifestation of the diasease. 2. The male to female ratio was 0.5:1 in type I, 1.1:1 in type II and 1.7:1 in newly diagnosed diabetics, and the mean age was 24.4 in type I, 57.9 in type II and 23.9 years old in newly diagnosed diabetics. 3. The mean duration between initial diagnosis of diabetes and the occurrence of DKA was 2.6 in type I and 6.9 years in type II diabetes. The occurrence of DKA within 2 years of initial diagnosis of diabetes was 54% in type I and 18% in type II diabetes, but the occurrence of DKA after 5 years of initial diagnosis of diabetes was 17% in type I and 47% in type II diabetes. 4. The precipitating factors of DKA were identified in 88% in type I, 76% in type II and 38% in newly diagnosed diabetics, and the most common precipitating factor was omission of treatment in both type I and type II(type I: 56%, type II: 35%). 5. The altered mental status was correlated with incresed osmolality(p<0.05), but not with other laboratory values such as pH, bicabonate, glucose, anion gap and dehydration status(p<0.05). Conclusion: It is necessary to conduct early aggressive evaluation for early diagnosis and proper treatment of DKA, because DKA occurs in patients with prior history of type II diabetes and without prior diabetic history as well as patients with prior history of type I diabetes.

Molecular variability and genetic structure of the population of <i>Soybean mosaic virus</i> based on the analysis of complete genome sequences

Seo, Jang-Kyun,Ohshima, Kazusato,Lee, Hyeok-Geun,Son, Moonil,Choi, Hong-Soo,Lee, Su-Heon,Sohn, Seong-Han,Kim, Kook-Hyung Elsevier 2009 Virology Vol.393 No.1

<P><B>Abstract</B></P><P>The complete genomes of 30 <I>Soybean mosaic virus</I> (SMV) isolates and strains were sequenced in this study. Together with fourteen previously reported sequences, we analyzed the genetic structure of the SMV population. Analyses of genetic diversity showed that different genomic regions of SMV are under different evolutionary constraints and that there was no significant genetic differentiation between East Asian and North American populations of SMV. Phylogenetic analyses revealed a significant correlation between phylogeny of the cylindrical inclusion (CI) gene of SMV and SMV resistance gene 3 (<I>Rsv3</I>)-relating pathogenicity of SMV, suggesting CI might be a pathogenic determinant in <I>Rsv3</I>-mediated disease response. Interestingly, recombination analyses identified 19 ‘clear’ recombination events in the SMV population. Furthermore, as several resistance-breaking strains were identified as recombinants, it appears that recombination might contribute to overcome host resistance in SMV–soybean pathosystem. Our finding suggests that recombination as well as mutation is an important evolutionary process in the genetic diversification of SMV population.</P>

자연기흉의 임상적 고찰

이정헌,서강석,김종근,윤영국 大韓應急醫學會 1997 대한응급의학회지 Vol.8 No.1

Spontaneous pneumothorax is an accumulation of air in the pleural space with collapse of the lungs in the absence of external chest trauma. In this study, 175 cases of spontaneous pneumothorax experienced at emergency room of Kyungpook University Hospital from July 1995 to June 1996 were analyzed retrospectively. The results were summarized follows: 1. Sex ratio of male to female was 6.6:1 and the mean age was 36.7 years. 2. In clinical symptoms, dyspnea was 85 cases(48.6%), chest pain 67 cases(38.3%), chest discomfort 18 cases(10.3%), coughing 2 cases(1.1%), and others 3 cases(1.7%). 3. In etiology of spontaneous pneumothorax, primary spontaneous pneumothorax was 118 cases (67.4%) and secondary spontaneous pneumothorax 57 cases(33.6%). The underlying pathologic lesion of secondary spontaneous pneumothorax showed pulmonary tuberculosis in 50 cases(28.6%), COPD in 5 cases(2.9%), bronchial asthma in 1 case(0.6%), and pneumoconiosis in 1 case(0.6%). 4. The incidence of recurrence was as follows; no previous history in 123 cases(70.3%), second attack in 35 cases(20.0%), third attack in 15 cases(8.6%), fourth attack in 1 case(0.6%), and fifth attack in 1 case(0.6%). 5. The interval of recurrence after last attack was as follows; less than 6 months in 36 cases(69.2%), between 6 minths and 1 year in 5 cases(9.6%), between 1 and 2 years in 3 cases(5.8%), between 2 and 3 years in 2 cases(3.8%), and greater than 3 years in 6 cases(11.5%). 6. In distribution of the lesion sites, left was 93 cases(53.1%), right 80 cases(45.7%), and bilateral 2 cases(1.1%). 7. In degree of lung collapse, 17 cases(9.7%) were small(<20%), 127 cases(72.6%) moderate(20-40%), and 31 cases(17.7%) large(>40%). 8. The accompanied diseases of spontaneous pneumothorax were found in 17 cases, in which hydrothorax were 14 cases and homothorax were 3 cases. 9. The employed management were as follows; bed rest with oxygen inhalation in 6 cases(3.4%), thoracentesis in 4 cases(2.3%), closed thoracostomy in 136 cases(77.7%), and open thoracotomy in 29 cases(16.6%). 10. The reexpansion time after chest tube insertion was as follows; less than 1 day in 147 cases(89.6%), between 1 and 2 days in 12 cases(7.3%), between 2 days and 1 week in 4 cases(2.4%), and greater than 1 week in 1 case(0.6%). 11. The duration of chest tube insertion was as follows; less than 1 week in 102 cases(62.2%), between 1 and 2 weeks in 34 cases(20.7%), between 2 and 3 weeks in 14 cases(8.5%), between 3 and 4 weeks in 7 cases(4.3%), and greater than 4 weeks in 7 cases(4.3%). 12. The complications of closed thoracostomy were as follows; incomplete lung expansion in 4 cases and empyema in 1 case. 13. The indications of open thoracotomy were as follows; bleb or bullae on chest X-ray in 12 cases(14.4%), persistent air leak in 8 cases(27.6%), recurrent pneumothorax in 4 cases(13.8%), incomplete lung expansion in 3 cases(10.3%), and contralateral pneumothorax history in 2 cases(6.9%). 14. The duration of hospitalization was as follows; less than 1 week in 100 cases(57.1%), between 1 and 2 weeks in 29 cases(16.6%), between 2 and 3 weeks in 22 cases(12.6%), between 3 and 4 weeks in 9 cases(5.1%), and greater than 4 weeks in 15 cases(8.6%).