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막힘온도 지시계를 이용한 액체 나트륨중 불순물 농도 측정
권상운,정경채,김병호,김광락,황성태,최윤동,정지영 한국공업화학회 1998 응용화학 Vol.2 No.2
The monitoring of impurity in sodium is one of the most important R&D issues to develop a liquid metal reactor. Impurity measuring experiments were carried out by a Plugging Temperature Indicator, which is an on-line impurity monitoring instrument. Plugging temperature was successfully measured at various oxygen contents with two operation modes- bare orifice mode and partially plugging mode. The relation between plugging temperature and oxygen content was calibrated from the experimental data. Further study is needed to develop a more reliable instrument.
Kwon, Seok J.,Lee, Geun T.,Lee, Jae-Ho,Kim, Wun J.,Kim, Isaac Y. Blackwell Publishing Ltd 2009 Immunology Vol.128 No.1
<P>Summary</P><P>Bone morphogenetic proteins (BMPs) are members of the transforming growth factor-&bgr; (TGF-&bgr;) superfamily. In the present study, we investigated the effect of BMPs on the production of inducible nitric oxide synthase (iNOS) in the murine macrophage cell line, RAW 264.7, and in mouse peritoneal macrophages. Among the BMPs, only BMP-6 induced iNOS expression in a time-dependent and dose-dependent manner in both cell types. Induction of iNOS was inhibited by both cycloheximide and actinomycin D, indicating that the induction of iNOS expression by BMP-6 requires new protein synthesis. Mechanistic studies revealed that the BMP-6-induced iNOS expression requires both Smads and nuclear factor-kappa B (NF-&kgr;B) signalling pathways. Furthermore, induction of interleukin-1&bgr; (IL-1&bgr;) was necessary for iNOS induction by BMP-6. These observations suggest that BMP-6 stimulates macrophages to produce iNOS through IL-1&bgr; via Smad and NF-&kgr;B signalling pathways and that BMP-6 may be an important regulator of macrophages.</P>
Deok Ho Kwon,Kyong Sup Yoon,J. Marshall Clark,Si Hyeock Lee 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.05
The molecular mechanisms and genetics of abamectin resistance mediated by target site insensitivity in the two-spotted spider mite, Tetranychus urticae, were investigated by comparing two isogenic AbaS and AbaR strains. Cloning and sequencing of full-length cDNA fragments of GABA-gated chloride channel genes revealed no polymorphisms between the two strains. However, sequence comparison of the full-length cDNA fragment of a T. urticae glutamate-gated chloride channel gene (TuGluCl) identified a G323D point mutation as being tentatively related with abamectin resistance. In individual F2 progenies obtained by backcrossing, the G323D genotype was confirmed to correlate with abamectin resistance. Bioassays using progeny from reciprocal crossings revealed that the abamectin resistance trait due to TuGluCl insensitivity is incompletely recessive.
Deok Ho Kwon,Keon mook Seong,Kyong Sup Yoon,J. Marshall Clark,Won Ja Lee,Yong Joon Ahn,Si Hyeock Lee 한국응용곤충학회 2008 한국응용곤충학회 학술대회논문집 Vol.2008 No.05
A quantitative sequencing (QS) protocol that detects the frequencies of sodium channel mutations (M815I, T917I and L920F) responsible for knockdown resistance in permethrin-resistant head lice was tested as a population genotyping method. Genomic DNA fragments of the sodium channel α-subunit gene that encompass the three mutation sites were PCR-amplified from individual head lice with either resistant or susceptible genotypes, and combined together in various ratios to generate standard DNA template mixtures for QS. Following sequencing, the signal ratios between resistant and susceptible nucleotides were calculated and plotted against the corresponding resistance allele frequencies. Quadratic regression coefficients of the plots were close to 1, demonstrating that QS is highly reliable for the prediction of resistance allele frequencies. Prediction of resistance allele frequencies by QS in several globally collected lice samples including 12 Korean lice populations suggested that permethrin resistance varied substantially amongst different geographical regions. Three local populations of Korean lice were determined to have 9.8-36.7% resistance allele frequencies, indicating that an urgent resistance management is needed. QS should serve as a preliminary resistance monitoring tool for proper management strategies by allowing early resistance detection.