Choline dehydrogenase interacts with SQSTM1/p62 to recruit LC3 and stimulate mitophagy.

Park, Sungwoo,Choi, Seon-Guk,Yoo, Seung-Min,Son, Jin H,Jung, Yong-Keun Landes Bioscience 2014 AUTOPHAGY Vol.10 No.11

<P>CHDH (choline dehydrogenase) is an enzyme catalyzing the dehydrogenation of choline to betaine aldehyde in mitochondria. Apart from this well-known activity, we report here a pivotal role of CHDH in mitophagy. Knockdown of CHDH expression impairs CCCP-induced mitophagy and PARK2/parkin-mediated clearance of mitochondria in mammalian cells, including HeLa cells and SN4741 dopaminergic neuronal cells. Conversely, overexpression of CHDH accelerates PARK2-mediated mitophagy. CHDH is found on both the outer and inner membranes of mitochondria in resting cells. Interestingly, upon induction of mitophagy, CHDH accumulates on the outer membrane in a mitochondrial potential-dependent manner. We found that CHDH is not a substrate of PARK2 but interacts with SQSTM1 independently of PARK2 to recruit SQSTM1 into depolarized mitochondria. The FB1 domain of CHDH is exposed to the cytosol and is required for the interaction with SQSTM1, and overexpression of the FB1 domain only in cytosol reduces CCCP-induced mitochondrial degradation via competitive interaction with SQSTM1. In addition, CHDH, but not the CHDH FB1 deletion mutant, forms a ternary protein complex with SQSTM1 and MAP1LC3 (LC3), leading to loading of LC3 onto the damaged mitochondria via SQSTM1. Further, CHDH is crucial to the mitophagy induced by MPP+ in SN4741 cells. Overall, our results suggest that CHDH is required for PARK2-mediated mitophagy for the recruitment of SQSTM1 and LC3 onto the mitochondria for cargo recognition.</P>

김치유래 젖산균의 균체지방산 분석을 이용한 분류학적 연구

이정숙,정민철,김우식,이근철,김홍중,박찬선,이헌주,주윤정,이근종,안종석,박완,박용하,민태익 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

표준균주를 포함한 230여개의 김치유래 젖산균에 대한 균체지방산(FAMEs)을 분석하였다. FAMEs profiles는 Euclidian Distance 17.5에 의해 7개의 Major Cluster와 1개의 Single Cluster로 나뉘어졌다. 이중 A, B, C 및 Cluster는 Leuconostoc속으로 분석되어졌고, F는 Lactobacillus속으로 분석되어졌다. 그리고 E와 G cluster는 두개의 Genus가 혼재되어 나타났으며 보충적인 연구가 필요하다. 앞으로 김치유래 젖산균의 균체지방산 분석결과를 기반으로 한 데이타베이스에 95가지 탄소원을 이용하는 수치분류학적 접근방법 및 Pyrolysis Mass Spectrometry 등의 화학적 분석 방법과 분자친화적 연구를 통한 종합적 분류정보 체계가 갖추어지면 젖산균의 신속, 정확한 동정 및 연구에 활발히 이용되어질 것이다. Two hundreds and thirty lactic acid bacteria, mostly isolated from Kimchi, including type strains were sued for analysis of cellular fatty acids. The 230 test strains were recoverd in 7 major and 1 single clusters defined a Euclidian distance of 17.5. These aggregate taxa were equivalent to the genus Leuconostoc (aggregate group A, B, C and D), and the genera Leuconostoc and Lactobacillus (aggregate group G). It is concluded as evident that FAMEs (Fatty Acid Methyl Esters) profile of cell can be used as a criterion in classification of lactic acid bacterial from kimchi. Additional comparative taxonomic studies need to be carried out on well chosen representative strains to determine the most appropriate methods of value.

당 첨가량에 따른 송이정과의 물성 및 관능 특성

박미란 ( Mi Lan Park ),최수근 ( Soo Keun Choi ),정인창 ( In Chang Jung ),변광인 ( Gwang In Byun ) 한국식생활문화학회 2006 韓國食生活文化學會誌 Vol.21 No.6

지리산국립공원 심원계곡 생태경관 복원공사지역 식생 변화

정태준 ( Tae-jun Jung ),김연경 ( Yeon-Gyeong Kim ),김영진 ( Young-jin Kim ),정명희 ( Myung-hee Jung ),박경희 ( Kyoung-hee Park ),신창근 ( Chang-keun Shin ),박선홍 ( Seung-hong Park ),김영선 ( Young-sun Kim ) 한국환경생태학회 2021 한국환경생태학회지 Vol.35 No.3

본 연구는 지리산국립공원 심원계곡 생태경관 복원공사지역의 모니터링 결과를 분석하여 체계적인 복원관리를 도모할 수 있는 기초자료를 확보하는데 그 목적이 있다. 복원사업이 완료된 2017년도에 식생 모니터링을 위한 조사구 12개소와 대조구 4개소를 설치하였고, 2017년과 2020년의 상대우점치, 종다양도지수, 유사도 등의 변화를 분석하였다. 복원사업 시 수목이 식재되었던 조사구는 종다양도지수가 0∼1.4552로 나타났고, 대조구와의 유사도지수에서는 1개 조사구가 1.32%로 분석된 것을 제외하고 모두 0%로 나타났다. 조사구의 종다양도지수 및 유사도지수가 매우 낮은 것은 복원사업 시 식재된 수목이 고사하거나 멧돼지 피해, 유수에 의한 침식으로 유실되는 등의 영향으로 판단되었다. 반면 복원사업 시 수목이 식재되지 않은 미식재 조사구는 종다양도지수가 0.9538∼2.3222로 나타났고, 유사도지수는 최대 8.33%까지 분석되었다. 국립공원 생태경관 복원공사지역의 복원 공법 개발을 위한 장기 모니터링과, 미식재 조사구의 천이과정을 분석하는 지속적인 연구가 필요하다. This study aims to obtain basic data for systematic restoration by analyzing the monitoring results of the Shimwon Valley Ecological Landscape Restoration Project area in Jirisan National Park. In 2017, when the restoration project was completed, 12 monitoring plots and 4 control plots were installed for vegetation monitoring, and changes in the relative dominance, species diversity index and similarity between 2017 and 2020 were analyzed. The species diversity index of the surveyed areas where trees were planted during the restoration project was 0-1.4552, and the similarity index with the control group was 0% except for one survey area at 1.32%. The very low species diversity index and similarity index in the survey areas were attributed to the loss of trees planted during the restoration project due to death, damage by wild boars, or erosion by running water. On the other hand, the species diversity index was 0.9538-2.3222 in the monitoring plot where no tree was planted, and the similarity index was analyzed to be as high as 8.33%. It is necessary to continue the long-term monitoring for the development of ecological landscape restoration methods in the national park and analysis of the succession in monitoring plots where no trees were planted.

輸入開放에 對應한 中長期 全北地域 農業政策方向에 關한 硏究

朴正根,徐東均 全北大學校 1992 論文集 Vol.34 No.-

This paper attempts to analyze the direction of agricultural polices of Chonbuk region in the trade liberalization and to suggest an alternative regional policy of agricultural development in Chonbuk province. The increasing pressure of agricultural import liberalization from abroad has given great burden to Chonbuk agricultural sector which has lagged behind the average growth of national agriculture. The charactersitics of Chonbuk agriculture can be summarized as wide-spread small scale farming, greater portion of old-aged labor and rice monoculture system. However, the agricultural production sector of Chonbuk becomes more and more commercialized and the consumption patterns of agricultural products become diversified and high-quality oriented continuously. The present situation of Chonbuk agricultural is in a vulnerable state to those changes. Divergent measures in Chonbuk agriculture are urgently requested for the improvement of present production structure. Among these, the increase of Chonbuk local government investment for various in infra-structure of agricultural facilitis should be carried out in such a way that agricultural productivies can be enhansed. Furthermore, it is also important to emphasize on fostering specific activities that develops an appropriate technologies induced by changes of resource endowments.

결핵성 육아종에서 Thioredoxin peroxidase-2 의 발현

박근호,유형륜,정영진,윤기중,한원철,유대열,문형배 圓光大學校 醫科學硏究所 1999 圓光醫科學 Vol.15 No.2

Background: Thioredoxin peroxidase(TPX) is a kind of recently discovered antioxidant enzyme which react as rapid hydrogen ion donor for the removal of hydroperoxide. The action and distribution of the TPX was poorly understood in the human diseases. This experiments were designed for the study about the distribution of the TPX in the chronic granulomatous inflammation and about the correlation between the expression of TPX and the site of inflammation, histological activities of tuberculous inflammation or existence of mycobacterium in the inflammatory foci. Methods: The immunohistochemical stains were performed for the localization of the TPX-2 in the epithelioid cells, giant cells and lymphocytes in the chronic granulomatous inflammation. The tissue sections were obtained from the paraffin blocks of the 54 cases of tuberculosis (lung 21 cases, lymph node 12 cases, bone and soft tissue 12 cases, kidney 9 cases; active 33 cases, inactive 21 cases by the histologic classification; presence of mycobacterium 15 cases, no mycobacterium 39 cases by PCR reaction). Results: The expression of TPX-2 was 16.7% in the giant cells, 27.8% in the epithelioid cells and 100% in the lymphocytes of tuberculous inflammations. The expression of TPX-2 in the giant cells and epithelioid cells of the tuberculosis were 28.6% and 57.1% of the pulmonary tuberculosis; 33.3% in each cells of the renal tuberculosis; 0% in each cells of the lymph node or bone and soft tissue tuberculosis. The expression of TPX-2 in the giant cells and epithelioid cells were 9.1% in each cells of the active tuberculosis and were 28.6% and 57.1% in each cells of the inactive tuberculosis by histologic classification. The expression of TPX-2 in the giant cells and epithelioid cells was 40% in each cells of tuberculosis which mycobacteria were detected and the expression of TPX-2 was 7.7% and 23.1% in each cells which mycobacteria were not detected by PCR reaction in the paraffin embedded tissue. Conclusions: The above results were summarized that the TPX-2 in the giant cells and epithelioid cells were more frequently expressed in the inactive tuberculosis than in the active tuberculosis. These results suggest that the TPX-2 is a kind of regulating or suppressing factors in the activity of the tuberculosis.

LDI(LCD driver Ic)의 응답 시간을 향상시키기 위한 선행 강조 구동 회로 설계

박근형, 손홍희, 정인석 충북대학교 컴퓨터정보통신연구소 2008 컴퓨터정보통신연구 Vol.16 No.1

-본문참고-

광대역 이동통신 시스템에 적합한 채널추정 기법

박정근,박중후 漢陽大學校 工學技術硏究所 2002 工學技術論文集 Vol.11 No.-

파일럿 심볼을 이용하여 채널추정을 하는 통신시스템에서는 파일럿 심볼이 데이터 심볼과 시간적으로 다중화 되기 때문에 사용할 수 있는 파일럿 심볼의 수가 제한된다. 이러한 상황에서 파일럿 심볼만을 이용한 채널 추정은 일반적으로 좋은 성능을 보장할 수 없다. 최근에 연구되는 PADD(pilot-aided decision-directed) 알고리즘은 채널 추정을 위하여 파일럿 심볼과 데이터 심볼을 모두 사용하기 때문에 페이딩 채널 환경에서 파일럿 심볼만을 이용하여 채널을 추정하는 PA(pilot-aided) 알고리즘보다 우수한 성능을 보여준다. 본 논문에서는 기존의 PADD 알고리즘들을 분석하여 보다 개선된 구조의 PADD 알고리즘 구조를 제안하고 레일레이 다중 경로 페이딩 환경에서 모의실험을 실시하였다. 컴퓨터를 이용한 모의실험 결과를 통해 제안된 알고리즘이 기존의 알고리즘에 비해 성능이 향상됨을 확인할 수 있었다. In the communication systems that employ pilot-acid channel estimation techniques, pilot symbols are time-multiplexed with data symbols, and the number of pilot symbol is usually limited. It is known that PADD(pilot-aided decision-directed) approches exhibit better bit-error ratio (BER) performance than PA (pilot-aided) estimation algorithm over fading channels, because they use both pilot and data symbols for channel estimation. In this paper, conventional PADD algorithm are analyzed and an improved PADD algorithm for pilot symbol-acided channel estimations is proposed. The performance of this algorithm is evaluated in Rayleigh fading environments. Simulation results show that the proposed algorithm has better performance than conventional PADD algorithms.

소금이 마우스에서 고형암의 성장과 면역활성 및 지질과산화에 미치는 영향

정근옥,이강윤,이성갑,박건영 부산대학교 김치연구소 2002 김치의 과학과 기술 Vol.8 No.-

There are several types of salts, which are classified into raw salt (Chunil salt, CS), purified salt (PS) and processed salts (Gueun salt, Bamboo salt) in Korea. Gueun salt (Gs) is made by baking CS in the ceramic vessel at 800℃. Bamboo salt (BS) is produced by baking CS, bamboo and mud in an oven at 1,300℃. In this study, effects of these salts on tumor formation, NK (natural killer) cell activity and lipid peroxidation in kidney and heart were investigated in the sarcoma-180 cell transplanted mice. Sarcoma-180 cells were transplanted subcutaneously into the left groin of Balb/c mice. Then, various kinds of salt, including 1 time heat treated KCI mixed BS-A (CS : KCI =3:7)and B (CS : KCI = 5:5), supplemented diet at concentrations of 10% were fed for 21 days from 24 hours following transplantation. The body weight decreased when 10% salt added diet was fed to the sarcoma-180 cell treated Balb/c mice. The body weights of CS, PS and treated groups were lower than their initial weights (19 ~ 20 g). Spleen and liver index were lower in the mice administered PS and GS than sarcoma-180 cell treated control group. KCI mixed BS-A and B reduced the content of malondialdehyde (MDA) in the kidney and heart that increased by the injection of sarcoma-180 cells. The feeding of PS in the diet increased the levels of MDA in the kidney and heart of sarcoma-180 treated mice compared to the control group. These results exhibited that KCI mined BS could suppress.

全北農業生産의 成長 分析

朴正根 全北大學校 1977 論文集 Vol.19 No.-

This study attempts to analyize the growth patterns and the changes of agricultural structure in Jeonbug Province over the period from 1958 to 1975. The main objectives of this study have been to estimate the rates of total agricultual output and production of several commodity categories both during the period as a whole and in selected sub-periods. In this study major agricultural products are grouped under the following categories;rice, barleys, miscellaneous cereals, purlses, potatoes, special crops, vegetables, fruits, monopoly products, silkworm cocoons, and livestocks. All of the data which were relevant to this stndy were derived from the statistical year Book of Jeonbug Province from 1959 to 1976. The followings are the major findings. 1. From 1958 to 1975 the Jeonbug agricultural output grew at an annual compound rate of 4.0 percent. The compound annual rate was 7.4 percent from 1958 to 1963, 1.7 percent from 1963 to 1969 and 3.1 percent from 1969 to 1974. 2. If we compare the rates of annual growth of various products, miscellaneous cereals, potatoes and special crops showed unstable swing, while rice, purlses, fruits, vegetables and livestocks increased steadily, and silkworm cocoons and monopoly products have increased most impressibly. 3. It is quite clear that the growth of agricultural output was primarily depended upon the growth of the rice and barleys whose relative contribution accounted for more than one half of the growth of all output. 4. The differences in growth rates of production of the various farm products over the years have altered the relative composition of total agricultural products. With the increase in production of vegetables, fruits and livestocks, the share of rice has decreased. 5. The Location Quotients of rice, monopoly products and silkworm cocoons are more than one and other items show less than one.