Differential Manifestation of Ocular Phenotypes in TALEN-mediated p19arf Knockout FVB/N and C57BL/6J mouse lines

Jin‑Sung Park,Joo‑Il Kim,Hyun‑Jin Lim,Soo‑Kyung Ryu,Euna Kwon,Kang‑Min Han,Ki‑Taek Nam,Han‑Woong Lee,Byeong‑Cheol Kang 한국유전학회 2020 Genes & Genomics Vol.42 No.9

Background p19arf, primarily known as a tumor suppressor, has also been reported to play an essential role in normal development of mouse eyes. Consistently, lack of p19arf has been associated with ocular defects, but the mixed background of the knockout (KO) mouse strain used raised a concern on the accuracy of the phenotypes observed in association with the targeted gene due to genetic heterogeneity. Object We carried out a study to investigate into the efect of genetic background on the manifestation of p19arf KO associated phenotypes. Methods We characterized the phenotypes of novel p19arf KO mouse lines generated in FVB/N and C57BL/6J using a transcription activator-like efector nuclease (TALEN) system in comparison to the reported phenotypes of three other p19arf-defcient mouse lines generated using homologous recombination. Results Ninety-fve percent of FVB/N-p19arf KO mice showed ocular opacity from week 4 after birth which worsened rapidly until week 6, while such abnormality was absent in C57BL/6J-p19arf KO mice up to the age of 26 weeks. Histopathological analysis revealed retrolental masses and dysplasia in the retinal layer in FVB/N-p19arf KO mice from week 4. Besides these, both strains developed normally from birth to week 26 without increased tumorigenesis except for a subcutaneous tumor found in a C57BL/6J-p19arf KO mouse. Conclusion Our fndings demonstrated surprisingly variable manifestation of p19arf-linked phenotypes between FVB/N and C57BL/6J mice, and furthermore between our mouse lines and the established lines, indicating a critical impact of genetic background on functional study of genes using gene targeting strategies in mice.

심정지환자에서 epinephrine투여시 혈중 catecholamine의 변화

임경수,황성오,이부수,이강현,이진웅,임종천,김영식,김선만,김 현 대한응급의학회 1995 대한응급의학회지 Vol.6 No.1

Background : Cardiac arrest is a potent stimulus for the release of endogenous catecholamine and high plasma catecholamine concentration has been reported during cardiopulmonary resuscitation(CPR). Few data were present about the clinical effect of high plasma catecholamine and endogenous catecholamine response to exogenous epinephrine administration during CPR. Purpose : This study was designed to evaluate endogenous catecholamine response to cardiac arrest and exogenously administered epinephrine during CPR. Method : In 21 cardiac arrest victims, blood sample for epinephrine and norepinephrine was drawn before and 1 minute after each epinephrine administration during CPR. 1mg of epinephrine was administered immediately after intravenous access and 3 minute interval during CPR. Plasma catecholamines were quantitated by high-performance liquid chromatography. Result : Baseline plasma epinephrine concentraqtion was higher in patients with cardiac arrest than normal controls (985±1627 vs 194±173 pg/ml, p<0.05). Baseline plasma norepinephrine concentration was also elevated in patients with cardiac arrest than normal controls, although statistically insignificnat(1965±4915 vs 360±250 pg/ml). Plasma epinephrine concentration in cardiac arrest patients was significantly elevated after exogenous administration of epinephrine, but its response was blunted after the third does of epinephrine. Plasma norepinephrine concentration in cardiac arrest patients was also elevated, but its response was observed only after the first epinephrine administration. Plasma epinephrine concentration was persistently higher in patients with return of spontaneous circulation (ROSC) than patients without ROSC. there was no significant difference of plasma norepinephrine concentration whether spontaneous circulation was restored or not. Endogenous epinephrine response to exogenous epinephrine was not observed in patients having prolonged arrest time over 20 minutes. Conclusion : Results of this study suggests that significant amount of catecholamine is released by exogenous epinephrine administration in spite of high plasma catecholamine concentration, and degree of endogenous catecholamine response influences resuscitation outcome.

수정봉(충청남도)의 식물상

임수진,이웅빈 용인대학교 자연과학연구소 2009 自然科學硏究所論文誌 Vol.14 No.1

The investigated area is located 36° 44′ 50″∼36° 46′ 40″ N in latitude at 126° 36′ 20″∼126° 37′ 40″ E in longitude. The investigated vascular plants consist of total 126 taxa: 3 forms, 21 varieties, 102 species, 90 genera, 46 families, 25 orders, 4 classes. 6 special plants designated by Ministry of Environment were investigated: 4th grade for 2 taxa, 3rd grade for 2 taxa, 2nd grade for 2 taxa. There is two taxa korean endemic plant that is Lespedeza thunbergii var. intermedia (Nak.) T. Lee, Saussurea eriophylla Nakai. As for the useful plants, there were 72 taxa of edible ones(57.1%), 59 taxa of medicinal ones(46.8%), 20 taxa of ornamental ones(15.9%), 50 taxa of industrial ones(39.7%), and 2 taxa of poisonous ones(1.6%). Naturalized plants were distributed 4 taxa.

무갑산(경기도)의 식물상

임수진,이웅빈 용인대학교 자연과학연구소 2008 自然科學硏究所論文誌 Vol.13 No.1

Abstract - Mt. Mugap is located 37°23′00″~37°25′00″N in latitude at 127°19′00″~127° 21′30″E in longitude in Gyeonggi-do. The investigated vascular plants consist of total 173 taxa: 3 forms, 21 varieties, 149 species, 133 genera, 63 families, 28 orders, 4 classes. Twelve special plants designated by Environment Ministry were investigated: 4th grade for 4 taxa, 3rd grade for 4 taxa, 2nd grade for 2 taxa, and 1st grade for 2 taxa. As for the useful plants, there were 79 taxa of edible ones(45.7%), 42 taxa of medicinal ones(24.2%), 11 taxa of ornamental ones(6.4%), 41 taxa of industrial ones(23.7%), and 4 taxa of poisonous ones(2.3%) respectively.

혼수상태의 외상환자에서 초음파를 이용한 흉부 및 복부손상의 진단

임경수,이강현,이진웅,이부수,황성오,유수영,강성준 대한응급의학회 1995 대한응급의학회지 Vol.6 No.1

The need for rapid diagnosis and treatment of life-threatening thoracic and intra-abdominal injury result in controversy over the appropriate triage of unconscious blunt trauma patients. To aid in early decisions for these patients, a prospective analysis of 98 patients with glasgow coma scale(GCS) scores≤8 was undertaken. Although intra-thoracic and intra-abdominal injuries were frequently identified based on systolic blood ressure, the use of clinical signs alone resulted in more missed injuries than did using the emergency ultrasonography. In normotensive patients(n=34), intra-thoracic injuries was identified in one patient(2.9%), and intra-abdominal injuries were in 7 patients(20.6%). In shock(systolic blood pressure<90mmHg) Pa-tients(n=64), thoracic injuries and intra-abdominal injuries were diagnosed in 8(12.5%) and 27 patients(42.3%), thoracic and abdominal combined injuries were identified in 11 patients(17.2%). The sensitivity, specificity, and accuracy of emergency ultrasonography were 96.3%, 91.7%, 94.9%. This study suggests that all unconscious trauma patients undergo immediate emergency ultrasonography to prevent missing life-threatening injuries.

간세포암의 크기와 알파 피토프로틴의 연관성

임효순,정용연,김재규,윤웅,강형근,박진균 全南大醫大 2001 전남의대학술지 Vol.37 No.4

외상후 혈뇨환자에서 응급 초음파검사의 의의

임경수,이강현,황성오,이진웅 대한응급의학회 1996 대한응급의학회지 Vol.7 No.2

The Ultrasonography is rapid, mobile, and noninvasive diagnostic tool in emergency center. As a result, we estimate it`s value as an advocated primary diagnostic modality in the immediate evaluation of patients with multiple trauma. We investigated 60 patients with traumatic hematuria(microscopic hematuria or gross hematuria) who visited our emergency department from 1995, July to 1996, March. To evaluate the injury of urinary systems, emergency physician examined patients with hematuria by ultrasonography, and comapared the results with those of excretory urography and computed tomography. We demonstrated 21 positive findings for abnormal fluid collection and renal parenchymal injury and bladder rupture on ultrasonography. All bladder injuries were diagnosised by ultrasonography, and all major renal injury(grade Ⅲ,Ⅳ,Ⅴ) were also diagnosised by that. The accuracy of emergency ultrasound examination in traumatic hematuria was 97.0%, and sensitivity was 95.0% with specificity of 97.4%. The ultrasonography was an accurate diagnostic adjunct in detecting renal injury and bladder injury in traumatic hematuria patients. Ultrasonography provides an excellent noninvasive diagnostic tool for the initial screening study of patients with traumatic hematuria.

다발성 외상환자에서 필수적 단순 방사선검사의 의의

임경수,황성오,임종천,이부수,이강현,이진웅 대한응급의학회 1995 대한응급의학회지 Vol.6 No.2

In the guidelines of Advanced Trauma Life Support(ATLS), essential plane roentgenograms (chest AP vIew, cervical spine lateral view and pelvis AP view) are recommanded when primary survey and resuscitation phase are finished in care of multiple injured patients. A prospective study was carried on 376 multiple trauma patients who admitted to Wonju Christian Hospital from July, 1995 to October. We analyzed clinical significance of essential X-ray in multiple trauma patients, and classified the patients by their status of mentality. The one is alert group when their Glasgow Coma Scale(GCS) is over 13 point, the other is disoriented mentality group when their GCS are below 12 point. The alert group was redivided by presence of clnical symptoms and/or signs, and clinical significances of essential X-ray were analyzed. Among the 376 patients, 330 patients are alert group that their GCS score is over 13 point and 46 patients are disoriented group who's GCS were below 12 point. The detection rate of abnormality in essential X-ray is higher in disoriented group than alert group(cervical-spine lateral view : 13.5% vs 3.0% cheat AP view : 0.4%, pelvis AP view : 0%). This study suggests that their are no needs of taking essential X-ray in patients who is alert in mental state and have no clinical symptoms and/or signs.

백학산(경북 상주)의 식물상

이웅빈,임수진 Yong-In University 2005 自然科學硏究所論文誌 Vol.10 No.1

The flora of Mt. Baekhaksan(615m) was surveyed in this study from the middle of May till the middle of October, 1999. The vascular plants collected from this mountain are as following: Totally 178 taxa those are 4 forms, 28 varieties, 146 species, 106 genera, 60 families, 28 orders, and 4 phyla.. There are 1 taxa in this mountain known distributed only in northern parts, and no taxa known distributed only in southern parts. And there are 7 taxa of special protected plants which was designated from Ministry Environment of Korea, those are 2 taxa of 4th grade, 1 taxon of 3rd grade, 4 taxa of 1st grade. There are 2 taxa needed more ecological study with detail that are Larix gmelini var. prinsipisruprechtii Pilger and Sorbus amurensis Koehne.

노인외상환자의 외상중증도계수와 동통감지력의 변화에 대한 임상적 고찰

이진웅,임경수,김영식,황성오,이강현,김선만,김 현 大韓應急醫學會 1996 대한응급의학회지 Vol.7 No.1

Because elderly individuals are participitating in social work as adult inspite of their physical handicapes such as hearing difficulty, visual difficulty and limit of motion, they are continually exposed to the risk of injury. Trauma is now fifth cause of death in elderly of over 65 years old, and the elderly suffer disproportionately high injury-related motality comparing with younger adults. In addition, sometimes elderly patients don`t complaint of pain on injured site because of high threshold to pain. We studied about occurence of neglected injury due to high threshold to pain in geriatric trauma patients. When we diagnosised injury that patients did not complaint of pain on injured site through physical examination and radiologic examination, we defined that as neglected injury. There were 8 (8%) geriatric patients who didn`t complaint of pain on injured site on admission to emergency center, but only 0.7% of adult patients had neglected injury. The severity was estimated by use of AIS(Abbreviated Injury Scale), and the severity of all neglected injuries were above AIS 2 point. The neglected injury were head injuries(4 in number), abdominal injury(1), pelvic injuries(2) and extremity injury(1). So if geriatric trauma patients were admitted to emergency center, we recommand full physical and radiological examinations although patients don`t complaint of pain.