Inhibition of acetylation of histones 3 and 4 attenuates aortic valve calcification

Jia Gu,Yan Lu,Menqing Deng,Ming Qiu,Yunfan Tian,Yue Ji,Pengyu Zong,Yongfeng Shao,Rui Zheng,Bin Zhou,Xiangqing Kong,Wei Sun 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

Aortic valve calcification develops in patients with chronic kidney disease who have calcium and phosphate metabolic disorders and poor prognoses. There is no effective treatment except valve replacement. However, metabolic disorders put patients at high risk for surgery. Increased acetylation of histones 3 and 4 is present in interstitial cells from human calcific aortic valves, but whether it is involved in aortic valve calcification has not been studied. In this study, we found that treating cultured porcine aortic valve interstitial cells with a high-calcium/high-phosphate medium induced calcium deposition, apoptosis, and expression of osteogenic marker genes, producing a phenotype resembling valve calcification in vivo. These phenotypic changes were attenuated by the histone acetyltransferase inhibitor C646. C646 treatment increased the levels of class I histone deacetylase members and decreased the acetylation of histones 3 and 4 induced by the high-calcium/high-phosphate treatment. Conversely, the histone deacetylase inhibitor suberoylanilide hydroxamic acid promoted valve interstitial cell calcification. In a mouse model of aortic valve calcification induced by adenine and vitamin D treatment, the levels of acetylated histones 3 and 4 were increased in the calcified aortic valves. Treatment of the models with C646 attenuated aortic valve calcification by restoring the levels of acetylated histones 3 and 4. These observations suggest that increased acetylation of histones 3 and 4 is part of the pathogenesis of aortic valve calcification associated with calcium and phosphate metabolic disorders. Targeting acetylated histones 3 and 4 may be a potential therapy for inoperable aortic valve calcification in chronic kidney disease patients.

Pathway and Network Analysis in Glioma with the Partial Least Squares Method

Gu, Wen-Tao,Gu, Shi-Xin,Shou, Jia-Jun Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.7

Gene expression profiling facilitates the understanding of biological characteristics of gliomas. Previous studies mainly used regression/variance analysis without considering various background biological and environmental factors. The aim of this study was to investigate gene expression differences between grade III and IV gliomas through partial least squares (PLS) based analysis. The expression data set was from the Gene Expression Omnibus database. PLS based analysis was performed with the R statistical software. A total of 1,378 differentially expressed genes were identified. Survival analysis identified four pathways, including Prion diseases, colorectal cancer, CAMs, and PI3K-Akt signaling, which may be related with the prognosis of the patients. Network analysis identified two hub genes, ELAVL1 and FN1, which have been reported to be related with glioma previously. Our results provide new understanding of glioma pathogenesis and prognosis with the hope to offer theoretical support for future therapeutic studies.

Preparation and Preliminary Evaluation of Macroporous Magnetic Agarose Particles for Bioseparation

Jia-Li Gu,Hong-Fei Tong,Lai-Yu Sun 한국생물공학회 2017 Biotechnology and Bioprocess Engineering Vol.22 No.1

Macroporous magnetic agarose particles (MMAPs) were prepared with calcium carbonate as the porogent by the water-in-oil suspension thermal regeneration method. MMAPs with good sphericity and appropriate particle size were obtained. The physical properties of the beads were determined and it was found that the water content (92.1%), porosity (94.4%) and mean pore diameter (120.1 nm) of the MMAPs were higher than those for the normal magnetic particles, indicating successful generation of macropores after calcium carbonate addition. Compared with normal magnetic particles, the mass transfer of biomolecules in MMAPs was remarkably enhanced. Finally, MMAPs were modified with 5-amino-benzimidazol (ABI) ligand and the adsorption capacity of IgG reached 153 mg/mL, higher than that of the normal magnetic particles (126 mg/mL). Moreover, adsorption behavior of MMAPs to IgG was little changed after twenty-five recycled use. Hence, MMAPs prepared herein showed great potential for bioseparation.

中ㆍ韓 "旁이" 故事比較硏究

고희가(Gu Xi Jia) 한국어문교육학회 2004 어문학교육 Vol.29 No.-

中韩梁祝传说比较研究

顧希佳(Gu Xi Jia) 동양한문학회(구 부산한문학회) 2005 동양한문학연구 Vol.21 No.-

「양축[梁山伯과 祝英臺]설화」는 젊은 남녀의 사랑과 죽음을 그린 아름다운 이야기로 중국 4대 民間說話 가운데 하나이다. 중국에서는 오랜기간에 걸쳐 설화, 희곡, 소설로 발전하였고, 현대에 들어와서 음악, 剪紙, 발레, 영화 등의 장르로 확산되어왔다. 또 이들을 기리는 梁山伯 祠堂이나 祝英臺 讀書臺 등 기념물도 각지에 있어 일종의 ‘梁祝文化’를 형성하였다. 중국은 1953년에 양축영화를 만들었는데, 「東方의 줄리엣」(周恩來 제의)이라는 이름을 붙여서 서양에 소개하기도 했다. 「양축설화」는 나중에 口碑文學과 文獻說話 두 가지 방식으로 한국에 전래되어 장기간 한국인이 애호하는 설화가 되었다. 본 논문에서는 중국과 한국의 양축 설화에 관한 여러 판본을 비교 연구하여 서로간의 동일점과 차이점을 밝혀내었다. 한국의 양축 설화에서는 “버들잎 띄우기[柳葉傳情]”와 “옷을 부식시켜 자결하기[腐衣殉情]” 등의 부분이 자주 나타나는데 반해 중국에서는 거의 보이지 않는다. 또 중국의 양축 설화에서는 “십팔리 이별[十八相送]”과 “누대의 만남[樓臺相會]” 대목이 유명한데 비해 한국의 이야기에서는 잘 나타나지 않는다. 중국에서는 완전하게 이야기하는 경우는 드물고 부분의 대목을 이야기하는 경우가 많다. 본 논문은 이상에서 보이는 차이가 왜 일어나는지에 대하여 그 원인을 분석하였다. 여기에 더하여 중국인과 한국인들이 오랫동안 「양축설화」를 널리 사 랑한 주요한 원인들을 찾아내었다. “여성이 남장하여 공부하기[化裝求 學]” 대목이 지니는 傳奇的 特徵, 反封建 思想, 남녀 주인공의 운명에 대한 抗拒가 특히 두드러지며, “무덤에 뛰어들어 나비 되기[合塚化蝶]” 대목이 지닌 끊임없이 살아 숨쉬는 生命觀은 작품의 주요한 매력이라 할 수 있다.

Sirolimus and Non-melanoma Skin Cancer Prevention after Kidney Transplantation: A Meta-analysis

Gu, Yu-Hong,Du, Jia-Xin,Ma, Man-Ling Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.9

Background: Whether sirolimus is useful in the prevention of non-melanoma skin cancer (NMSC) remains unclear and we therefore performed this meta-analysis of randomized controlled trials to test the hypothesis that Sirolimus-based immunosuppression is associated with a decrease in NMSC. Methods: The main outcomes were NMSC, squamous-cell carcinoma and basal-cell carcinoma. The pooled risk ratio (RR) with its 95% confidence interval (95%CI) were used to assess the effects. Results: 5 randomized trials involving a total of 1499 patients receiving kidney transplantation were included. Patients undergoing Sirolimus-based immunosuppression had much lower risk of NMSC (RR = 0.49, 95%CI 0.32-0.76, P = 0.001). Subgroup analyses by tumor type showed that Sirolimus-based immunosuppression significantly decreased risk of both squamous-cell carcinoma (RR = 0.58, 95%CI 0.43-0.78, P < 0.001) and basal-cell carcinoma (RR = 0.56, 95%CI 0.37-0.85, P = 0.006). The quality of evidence was high for NMSC, and moderate for squamous-cell carcinoma and basal-cell carcinoma. No evidence of publication bias was observed. Conclusion: High quality evidence suggests that Sirolimus-based immunosuppression decreases risk of non-melanoma skin cancer, and Sirolimus has an antitumoral effect among kidney-transplant recipients.

Computational design towards energy efficient optimization in overconstrained robotic limbs

Gu Yuping,Wang Ziqian,Feng Shihao,Sun Haoran,Lu Haibo,Pan Jia,Wan Fang,Song Chaoyang 한국CDE학회 2023 Journal of computational design and engineering Vol.10 No.5

Legged robots are constantly evolving, and energy efficiency is a major driving factor in their design. However, combining mechanism efficiency and trajectory planning can be challenging. This work proposes a computational optimization framework for optimizing leg design during basic walking while maximizing energy efficiency. We generalize the robotic limb design as a four-bar linkage-based design pool and optimize the leg using an evolutionary algorithm. The leg configuration and design parameters are optimized based on user-defined objective functions. Our framework was validated by comparing it to measured data on our prototype quadruped robot for forward trotting. The Bennett robotic leg was advantageous for omni-directional locomotion with enhanced energy efficiency.

Dynamic sensitivity analysis and optimum design of aerospace structures

Gu, Yuanxian,Kang, Zhan,Guan, Zhenqun,Jia, Zhiwen Techno-Press 1998 Structural Engineering and Mechanics, An Int'l Jou Vol.6 No.1

The research and applications of numerical methods of design optimization on structural dynamic behaviors are presented in this paper. The emphasis is focused on the dynamic design optimization of aerospace structures, particularly those composed of composite laminate and sandwich plates. The methods of design modeling, sensitivity analysis on structural dynamic responses, and the optimization solution approaches are presented. The numerical examples of sensitivity analysis and dynamic structural design optimization are given to demonstrate the effectiveness of the numerical methods.

Comparison of Liver Transplantation and Liver Resection for Hepatocellular Carcinoma Patients with Portal Vein Tumor Thrombus Type I and Type II

Jia-Yu Lv,Ning-Ning Zhang,Ya-Wei Du,Ying Wu,Tian-Qiang Song,Ya-Min Zhang,Yan Qu,Yu-Xin Liu,Jie Gu,Ze-Yu Wang,Yi-Bo Qiu,Bing Yang,Da-Zhi Tian,Qing-Jun Guo,Li Zhang,Ji-San Sun,Yan Xie,Zheng-Lu Wang,Xin 연세대학교의과대학 2021 Yonsei medical journal Vol.62 No.1

Purpose: The aim of this study was to compare the efficacy of liver transplantation (LT) and liver resection (LR) for hepatocellularcarcinoma (HCC) patients with portal vein tumor thrombus (PVTT) and to investigate risk factors affecting prognosis. Materials and Methods: A total of 94 HCC patients with PVTT type I (segmental PVTT) and PVTT type II (lobar PVTT) were involvedand divided into LR (n=47) and LT groups (n=47). Recurrence-free survival (RFS) and overall survival (OS) were comparedbefore and after inverse probability of treatment weighting (IPTW). Prognostic factors for RFS and OS were explored. Results: Two treatment groups were well-balanced using IPTW. In the entire cohort, LT provided a better prognosis than LR. Among patients with PVTT type I, RFS was better with LT (p=0.039); OS was not different significantly between LT and LR(p=0.093). In subgroup analysis of PVTT type I patients with α-fetoprotein (AFP) levels >200 ng/mL, LT elicited significantly longermedian RFS (18.0 months vs. 2.1 months, p=0.022) and relatively longer median OS time (23.6 months vs. 9.8 months, p=0.065). Among patients with PVTT type II, no significant differences in RFS and OS were found between LT and LR (p=0.115 and 0.335,respectively). Multivariate analyses showed treatment allocation (LR), tumor size (>5 cm), AFP and aspartate aminotransferase(AST) levels to be risk factors of RFS and treatment allocation (LR), AFP and AST as risk factors for OS. Conclusion: LT appeared to afford a better prognosis for HCC with PVTT type I than LR, especially in patients with AFP levels>200 ng/mL.

Effect of Stewing Time on the Small Molecular Metabolites, Free Fatty Acids, and Volatile Flavor Compounds in Chicken Broth

Rong Jia,Yucai Yang,Guozhou Liao,Yuan Yang,Dahai Gu,Guiying Wang 한국축산식품학회 2024 한국축산식품학회지 Vol.44 No.3

Chicken broth has a taste of umami, and the stewing time has an important effect on the quality of chicken broth, but there are fewer studies on the control of the stewing time. Based on this, the study was conducted to analyze the effects of different stewing times on the sensory, small molecular metabolites, free fatty acids, and volatile flavor compounds contents in chicken broths by liquid chromatography-quadrupole/timeof- flight mass spectrometry, gas chromatography-mass spectrometry, headspace solidphase microextraction, and gas chromatography-mass spectrometry. Eighty-nine small molecular metabolites, 15 free fatty acids, and 86 volatile flavor compounds were detected. Palmitic and stearic acids were the more abundant fatty acids, and aldehydes were the main volatile flavor compounds. The study found that chicken broth had the best sensory evaluation, the highest content of taste components, and the richest content of volatile flavor components when the stewing time was 2.5 h. This study investigated the effect of stewing time on the quality of chicken broth to provide scientific and theoretical guidance for developing and utilizing local chicken.