A novel mechanism for the pyruvate protection against zincinduced cytotoxicity: mediation by the chelating effect of citrate and isocitrate

Jee-Won Sul,Tae-Youn Kim,Hyun Ju Yoo,Jean Kim,Young-Ah Suh,Jung Jin Hwang,Jae-Young Koh 대한약학회 2016 Archives of Pharmacal Research Vol.39 No.8

Intracellular accumulation of free zinc contributesto neuronal death in brain injuries such as ischemiaand epilepsy. Pyruvate, a glucose metabolite, has beenshown to block zinc neurotoxicity. However, it is largelyunknown how pyruvate shows such a selective andremarkable protective effect. In this study, we sought tofind a plausible mechanism of pyruvate protection againstzinc toxicity. Pyruvate almost completely blocked corticalneuronal death induced by zinc, yet showed no protectiveeffects against death induced by calcium (ionomycin,NMDA) or ferrous iron. Of the TCA cycle intermediates,citrate, isocitrate, and to a lesser extent oxaloacetate, protectedagainst zinc toxicity. We then noted with LC–MS/MS assay that exposure to pyruvate, and to a lesser degreeoxaloacetate, increased levels of citrate and isocitrate,which are known zinc chelators. While pyruvate addedonly during zinc exposure did not reduce zinc toxicity,citrate and isocitrate added only during zinc exposure, asdid extracellular zinc chelator CaEDTA, completelyblocked it. Furthermore, addition of pyruvate after zincexposure substantially reduced intracellular zinc levels. Our results suggest that the remarkable protective effect ofpyruvate against zinc cytotoxicity may be mediated indirectlyby the accumulation of intracellular citrate andisocitrate, which act as intracellular zinc chelators.

Establishment and characterization of bortezomib-resistant U266 cell line: Constitutive activation of NF-κB-mediated cell signals and/or alterations of ubiquitylation-related genes reduce bortezomib-induced apoptosis?

( Ju Won Park ),( Eun Kyung Bae ),( Chan Su Lee ),( Jee Hye Choi ),( Woo June Jung ),( Kwang Sung Ahn ),( Sung Soo Yoon ) 생화학분자생물학회 2014 BMB Reports Vol.47 No.5

Bortezomib has been known as the most promising anti-cancerdrug for multiple myeloma (MM). However, recent studiesreported that not all MM patients respond to bortezomib. Toovercome such a stumbling-block, studies are needed to clarifythe mechanisms of bortezomib resistance. In this study, weestablished a bortezomib-resistant cell line (U266/velR), andexplored its biological characteristics. The U266/velR showedreduced sensitivity to bortezomib, and also showed crossresistanceto the chemically unrelated drug thalidomide. U266/velR cells had a higher proportion of CD138 negative subpopulation,known as stem-like feature, compared to parentalU266 cells. U266/velR showed relatively less inhibitory effectof prosurvival NF-κB signaling by bortezomib. Further analysisof RNA microarray identified genes related to ubiquitinationthat were differentially regulated in U266/velR. Moreover, theexpression level of CD52 in U266 cells was associated withbortezomib response. Our findings provide the basis for developingtherapeutic strategies in bortezomib-resistant relapsed andrefractory MM patients.[BMB Reports 2014; 47(5): 274-279]

IL-17A promotes Helicobacter pylori-induced gastric carcinogenesis via interactions with IL-17RC

Jee-Hyun Kang,Suyoung Park,Eun-Ju Hong,Young-Suk Won,Hyo-Jung Kwon 한국실험동물학회 2022 한국실험동물학회 학술발표대회 논문집 Vol.2022 No.7

Metastatic Endometrial Cancer Manifesting as a Large Posterior Mediastinal Tumor Compressing Large Airways

( Jee Yea Choi ),( Joonkyung Kim ),( Min Ju Jo ),( Sung Jun Chung ),( Yoomi Yeo ),( Tai Sun Park ),( Dong Won Park ),( Ji-yong Moon ),( Sang-heon Kim ),( Tae Hyung Kim ),( Jang Won Sohn ),( Ho Joo Yoo 대한결핵 및 호흡기학회 2020 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.128 No.-

Most posterior mediastinal malignancies in adults are of neurogenic origin. Accordingly, metastatic posterior mediastinal tumors are very rare. We report a case where a metastatic endometrial cancer developed in the posterior mediastinum and compress the lower trachea and both main bronchi. A 71-year-old woman who had received concurrent chemoradiation for stage IV endometrial cancer 13 years ago was admitted for progressive dyspnea. A 10 cm-sized posterior mediastinal tumor compressing the lower trachea and both main bronchi was found on the chest computed tomography. Positron tomography showed no metastasis to organs other than the posterior mediastinum. For pathologic confirmation, we performed bronchoscopy and EBUS-TBNA. After the procedure, the patient developed respiratory failure and received mechanical ventilation. The patient was diagnosed with metastatic endometrial adenocarcinoma based on pathologic findings (positive for p16 and TTF-1). She received concurrent chemoradiotherapy to treat metastatic endometrial cancer compressing the airways while receiving mechanical ventilator treatment. Thereafter, the patient can be successfully weaned from the ventilator. To the best of our knowledge, this is the first case in which previous endometrial cancer recurred as a posterior mediastinal tumor without the involvement of other abdominal organs or lungs. Although it is very rare, clinicians should be aware that metastatic cancer can manifest as a single posterior mediastinal tumor and can cause respiratory failure by compressing large airways.

Outcome and Safety of Nivolumab in Real Life in the Treatment of Far-Advanced Hepatocellular Carcinoma: A Single Center Experience

( Jee Hee Park ),( Joong-won Park ),( Bo Hyun Kim ),( Ju Hee Lee ),( Young Hwan Koh ),( Chang-min Kim ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1

Aims: Sorafenib has been the only approved systemic agent for advanced hepatocellular carcinoma (HCC) for the past decade and multiple new drugs were recently approved. Out of these, nivolumab, a programmed cell death protein-1 inhibitor, is the first immune checkpoint inhibitor approved for the second-line therapy for advanced HCC. The aim of this study is to describe our experience with nivolumab in patients who have progressed on or been intolerant of prior sorafenib. Methods: Thirty consecutive patients with advanced HCC receiving nivolumab were enrolled between October 2017 and March 2018. Enrolled patients were given nivolumab 3mg/ kg intravenously every 2 weeks. Assessment of response was based on the Immune-Modified Response Evaluation Criteria In Solid Tumors (iRECIST) and RECIEST version 1.1 every 8 weeks. Results: The median age of patients was 65.5 years (range, 46-81 years) and the predominant etiology was hepatitis B (64%). Most patients had preserved liver function (Child-Pugh class A, 93%) and good performance status (ECOG score of 0 or 1, 100%). Portal vein invasion and extrahepatic spread was present in 54% and 67%, respectively. Sorafenib was given all patients as a first-line treatment. Before nivolumab treatment, 6 patients received 2 systemic agents and another 6 patients received 3 or more. As of the cutoff date of April 5 2018, the median duration of nivolumab treatment was 61 days. Eleven out of 30 patients (36.7%) are on treatment, while 19 patients stopped treatment because of disease progression (n=9, 30%), serious toxicity (n=7, 23.3%), treatment refusal (n=2) and follow up loss (n=1). Of 22 patients available for evaluating tumor response, 2 showed partial response (PR; 9.2%); 8 stable response (SD; 36.3%); 4 immune unconfirmed progression (iUPD; 18.2%) and 8 immune confirmed progression (iCPD; 36.3%). One of the four iUPDs was identified as iCPD and discontinued treatment, while three patients are still on treatment awaiting the next evaluation. Median overall survival (OS) was not reached because of short follow up duration (83 days). Mean OS was 117.7 days (95% confidence interval [CI]; 105.4-129.9) and median progression free survival (PFS) was 97 days (95% CI; 72.9-121.1). Survival rate and PFS rate at 3 months were 90.2% and 64.9%, respectively. Most common adverse reactions included abdominal discomfort (20%), general weakness (13%), constipation (13%), ascites (13%), skin eruption or pruritus (13%), anorexia (10%), nausea (10%), myalgia (7%), diarrhea (7%), peripheral edema (7%), fatigue (3%), dyspnea (3%). Treatment-emergent grade 3 adverse events (n=7) included hepatotoxicity, azotemia, hematochezia, anorexia, hyperbilirubinemia, and hypercalcemia, which necessitated treatment cessation. Conclusions: In real life practice of a single center, nivolumab as a second- or a third-fourth-line therapy appears to have meaningful efficacy and acceptable tolerability in patients with far-advanced HCC. Further follow-up studies are warranted.

Changes in Dimensional stability and Total Appearance Value (TAV) of Wool-blended Fused Fabrics after Pressing and or Dry Cleaning

Jee, Ju-Won 한국의류학회 2003 한국의류학회지 Vol.27 No.12

본 연구에서는 실제 의복 관리와 생산 면에서 접할 수 있는 모 직물의 접착심과의 접착후, 프레싱 처리후, 드라이클리닝 및 프레스로 처리 후 직물의 형태안정성과 의복형성능의 변화를 살펴보았다. 겉감으로는 신사복 춘하용 모 100% 또는 모 혼반 직물 15종류를 사용하였으며 심지로는 신사복에 많이 쓰이는 3종류 심지를 사용하였다. FAST시스템과 KES시스템을 이용하여 직물의 접착 후, 프레싱 처리후, 드라이크리닝과 프레싱 처리 후의 형태안정성과 의복형성능의 변화를 시험하였으며 다음과 같은 결과를 얻었다. 1) 모직물의 형태안정성에서 주용인자인 습윤팽창(HE)은 접착으로 크게 감소하였고 프레싱 처리와 드라이크리닝과 프레싱 처리 후는 접착 후보다 큰 변화를 보이지 않는다. 모직물의 습윤팽창(HE)의 조절시 접착심이 습윤팽창(HE)의 안정성에서 중요한 역할을 하는 것으로 나타났다. 2) 완화수축(RS)은 접착 후 조금 감소하였으나 큰 변화를 보이지 않고 프레싱 처리 후 현저한 감소를 보인다. 드라이크리닝과 프레싱 처리 후는 직물이 수축하고 볼륨감이 생김으로 완화수축(RS)은 더욱 감소하였다. 모직물의 완화수축(RS)의 조절시 프레싱이 중요한 역할을 하는 것으로 나타났다. 3) 모직물의 의복형성능(TAV)은 접착으로 크게 향상되었다. 드라이크리닝과 프레싱 처리 후 직물이 좀더 부드러워지고 볼륨감이 생기면서 의복형성능(TAV)은 적게 향상됨을 보인다. 이와 같이 3가지 처리는 서로 보완되어 모직물의 형태안정성과 의복형 성능을 향상시키는 것으로 나타났다.

Effects of 4-tert-octylphenol on glutathione-related antioxidant status in olive flounder Paralichthys olivaceus

Jee, Jung-Hoon,Keum, Yoo-Hwa,Bae, Jun Sung,Lee, Chae Won,Yang, Chan Yeong,Choi, Sang-Hoon,Kang, Ju-Chan,Park, Kwan Ha The Korean Society of Fish Pathology 2019 한국어병학회지 Vol.32 No.2

Effects on glutathione-related antioxidant parameters were examined after a chronic exposure of olive flounder, Paralichthys olivaceus to dietary 4-tert-octylphenol (4-tert-OP). Fish were fed diets containing 4-tert-OP at 0, 1, 5 and 10 mg/kg diet for 6 weeks. Antioxidant parameters examined were reduced glutathione (GSH) contents and enzyme activities of glutathione reductase (GR), glutathione S-transferase (GST) and glutathione peroxidase (GPx) in tissue homogenates of the liver, kidney and gill. It was observed that all parameters examined increased although there were some differences in dose responses and temporal patterns in the increase. GSH contents increased after exposure to 4-tert-OP in the three organs examined. However, the GSH increase was evident only after 4 weeks in the liver whereas it was elevated after 2 weeks in the kidney and gill. GR activity exhibited a significant increase in response to 4-tert-OP at 1 mg/kg in all three organs, however, its activity returned to control levels when exposed to 5 and 10 mg/kg. Hepatic GST activity showed an earlier increase at week 2 in contrast to the kidney and gill where they increased after 4 weeks of 4-tert-OP exposure. Temporal patterns in GPx activity changes to 4-tert-OP exposure were dissimilar among the organs: hepatic activity increased from week 2 through week 6; renal activity increased transiently at week 2; gill levels were higher through weeks 4 - 6. The results suggest that elevation of several GSH-related antioxidant parameters can be considered as evaluation criteria for 4-tert-OP-induced oxidative stress in a fish.

E2E 딥러닝 모델로 구현한 임베디드 환경의 명령어 음성인식 시스템

Jee-Yeop Back(백지엽),Jeong-Hwan Kim(김정환),Jun-Young Seo(서준영),Young-Ju Kweon(권영주),Sung-Dong Cho(조성동),Kang-Hwi Lee(이강휘),Chi-Won Choi(최치원) 대한전기학회 2020 정보 및 제어 심포지엄 논문집 Vol.2020 No.10

The Changes of Appearance Properties of Bamboo Knitted Fabric After Loess Dyeing

Jee, Ju-Won The Korean Home Economics Association 2007 International Journal of Human Ecology Vol.8 No.2

In this study, the mechanical properties related to the fabric appearance of well being functional bamboo knitted fabric before and after loess dyeing were evaluated. The mechanical properties of fabrics were measured by KES-F system, that is, shape retention, draping, wrinkle recovery, compression property, and surface properties, and total hand value of three types of knitted fabric, 100% bamboo, 100% cotton, and bamboo / cotton blend (60 / 40) were evaluated before and after loess dyeing. As a result, it was found that appearance density, shape retention, and drape coefficient of cotton knitted fabric were greater than those of bamboo knitted fabric. After loess dyeing, shape retention and drape coefficient, wrinkle recovery of bamboo knitted fabric improved and WC/C and MMD/SMD decreased. According to THV, the hand of bamboo/cotton blend knit is the best among three samples by compensation the weak properties of the two fiber. Therefore, loess dyeing seemed to be a good method for improving shape retention and hand value of 100% bamboo and bamboo blended fabric.

The Blockade of IL6 Counterparts the Osmolar Stress-Induced Apoptosis in Human Conjunctival Epithelial Cells

Ju, Hee-Jung,Byun, Yong-Soo,Mok, Jee-Won,Joo, Choun-Ki Hindawi Publishing Corporation 2016 Journal of ophthalmology Vol.2016 No.-

<P>To determine the effect of hyperosmolarity on cell survival/apoptosis of conjunctival epithelial cells and evaluate the possible role of IL6, Wong-Kilbourne derivative of Chang conjunctival cell line (WKD) was used in this study. Confluent cells were incubated under different osmolarity (290 mOsm and 500 mOsm) with or without neutralizing IL6 antibody (50 ng/mL). The expression of IL6 level was measured in the supernatant of each conditioned medium. Cell viability/apoptosis assay was performed using Annexin V/Propidium Iodide (PI) and Cell Counting Kit-8 (CCK-8). Western blot was conducted to measure the abundance of apoptotic markers and IL6 related downstream signaling pathway. The concentration of IL6 showed time-dependent increase in cells treated with 500 mOsm. Although apoptosis of WKD cell is increased in treated 500 mOsm for 24 h, apoptosis reduced in WKD cell treated 500 mOsm with anti-IL6 for 24 h. Anti-IL6 inhibited the activation of JAK-STAT signaling pathway, which was induced by hyperosmolarity. Hyperosmolar condition induced apoptosis in conjunctival epithelial cells, along with increase of IL6 production. IL6 neutralizing antibody inhibited apoptosis and JAK-STAT signaling in hyperosmolar condition. These findings suggested that IL6 may be involved in apoptotic change and in hyperosmolarity.</P>