혈액투석중인 만성신부전 환자에서 골대사 지표로써의 Osteocalcin치

송치운,이진홍,안미애,윤환중,윤상임,성기양,이강현,송민호,이강욱,신영태,김영건,노흥규 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.2

Background : Serum osteocalcin is synthesized by osteoblast and has been shown to be sensitive indicator of bone turnover inpatients with various metabolic bone disease. In renal osteodystrophy, serum osteocalcin is elevated due to decreased renal clearance and elevated level of PTH. This study was done to evaluate the usefulness of serum osteocalcin as a marker of bone metabolism and the correlation with other biochemical markers of bone metabolism. Methods : We measured serum osteocalcin, calcium, phosphorus, ALP(alkaline phosphatase) and PTH(parathyroid hormone) in 37 patients with end stage renal disease on hemodialysis. Osteocalcin was determined by radioimmunoassay and PTH was determined by radioimmunometric assay. Results : 1) The mean level of serum osteocalcin in ESRD patients was 233.8± 218.2ng/ml which was significantly higher than that of controls(p<0.0001). 2) The mean level of serum PTH in ESRD patients was 40.5± 43.8pg/ml was significantly higher than that of controls(p<0.005). 3) There was a significant positive correlation between the level of serum PTH, ALP and the level of serum osteocalcin in ESRD patients. 4) By using multiple regression, PTH is most reliable factor that affect to elevated level of serum osteocalcin ( beta coefficient = 0.687, Sig T<0.05). Conclusion : Serum osteocalcin as a marker of bone metabolism in ESRD patients is more useful than other biochemical marker such as serum calcium, phosphorus, ALP and PTH is a most reliable factor that affect to elevated level of serum osteocalin.

Partially Depleted SONOS FinFET for Unified RAM (URAM)—Unified Function for High-Speed 1T DRAM and Nonvolatile Memory

Han, Jin-Woo,Ryu, Seong-Wan,Kim, Chung-Jin,Kim, Sungho,Im, Maesoon,Choi, Sung Jin,Kim, Jin Soo,Kim, Kwang Hee,Lee, Gi Sung,Oh, Jae Sub,Song, Myeong Ho,Park, Yun Chang,Kim, Jeoung Woo,Choi, Yang-Kyu IEEE 2008 IEEE electron device letters Vol.29 No.7

<P> Unified random access memory (URAM) is demonstrated for the first time. The novel partially depleted (PD) SONOS FinFET provides unified function of a high-speed capacitorless 1T DRAM and a nonvolatile memory (NVM). The combination of an oxide/nitride/oxide (O/N/O) layer and a floating-body facilitates URAM operation in PD SONOS FinFETs. An NVM function is achieved by FN tunneling into the O/N/O stack and, a 1T-DRAM function is achieved by excessive-hole accumulation in the PD body. The fabricated PD SONOS FinFET shows retention time exceeding 10 years for NVM operation and program/erase time below 6 ns for 1T-DRAM in a single-cell transistor. These two memory functions are guaranteed without disturbance between them. </P>

A Study on Statistical Analysis of Chemical Accident Cases - Busan, Ulsan, and Gyeongnam Areas -

Kyu Yeol Lee,Ji Hun Jo,Seon Oh Park,Jin Woo Heo,Yong Sun Im 위기관리 이론과 실천 2019 Crisisonomy Vol.15 No.7

본 연구는 2013년부터 2018년까지 총 6년간의 부산⋅울산⋅경남 지역 화학사고 발생 현황을 대상으 로 지역적 특색에 따른 화학사고를 다각도로 분석 하였다. 연도별 분석결과 6년간 총 87건, 연평균 14.5건의 화학사고가 발생하였다. 사고유형으로는 시설관리미흡이 40.2%, 작업자부주의가 37.9%, 운송사고가 21.8%로 나타났다. 지역별 분석결과 부산지역 26건, 울산지역 39건, 경남지역이 22건으 로 나타났다. 연간 화학사고의 분석결과 7월이 가장 높고, 12월이 가장 낮은 분포를 보였다. 계절별 로는 여름철(40.2%)이 가장 높은 사고분포를 나타내었고, 요일별 분석결과 월요일(20.7%), 목요일 (18.4%)에 사고발생 비율이 높았다. 하루 중에는 9~11시(19.5%), 13~15시(18.4%)의 사고분포가 높게 나타났다. 물질별 분석결과 사고 원인의 유해화학물질은 암모니아(23.2%), 황산(14.5%), 염소 (10.1%), 질산(8.7%) 등의 순으로 높게 나타났다. 연구기간 중 화학사고에 의한 인명피해는 사망자 9명, 부상자 57명으로 나타났다. 이와 같은 연구는 국민의 안전을 위한 화학물질 관리 정책에 중요한 기초자료가 될 것이다. This study covers the present state of chemical accidents in Busan, Ulsan and Gyeongnam areas in South Korea for 6 years from 2013 to 2018. It analyzes a total of 87 chemical accidents (an average of 14.5 accidents annually) according to regional characteristics in various angles. The types of accidents include insufficient facility management (40.2%), inadvertent worker activity (37.9%), and transportation vehicle accidents (21.8%). According to regional analysis, 26 cases were found in Busan, 39 cases in Ulsan and 22 cases in Gyeongnam areas. The rate of accidents was high on Monday (20.7%) and Thursday (18.4%) as well as 9-11 AM (19.5%) and 1-3 PM (18.4%) during the day. The most frequent toxic chemical substances causing the accident was found ammonia (23.2%), followed by sulfuric acid (14.5%), chlorine (10.1%) and nitric acid (8.7%). Nine people died and 57 people were injured from the chemical accidents. It is expected that this research provides an important basis for the chemical management policies in South Korea.

In vivo visualization and monitoring of viable neural stem cells using noninvasive bioluminescence imaging in the 6-hydroxydopamine-induced mouse model of Parkinson disease.

Im, Hyung-Jun,Hwang, Do Won,Lee, Han Kyu,Jang, Jaeho,Lee, Song,Youn, Hyewon,Jin, Yeona,Kim, Seung U,Kim, E Edmund,Kim, Yong Sik,Lee, Dong Soo MIT Press 2013 Molecular imaging Vol.12 No.4

<P>Transplantation of neural stem cells (NSCs) has been proposed as a treatment for Parkinson disease (PD). The aim of this study was to monitor the viability of transplanted NSCs expressing the enhanced luciferase gene in a mouse model of PD in vivo. The PD animal model was induced by unilateral injection of 6-hydroxydopamine (6-OHDA). The behavioral test using apomorphine-induced rotation and positron emission tomography with [18F]N-(3-fluoropropyl)-2'-carbomethoxy-3'-(4-iodophenyl)nortropane ([18F]FP-CIT) were conducted. HB1.F3 cells transduced with an enhanced firefly luciferase retroviral vector (F3-effLuc cells) were transplanted into the right striatum. In vivo bioluminescence imaging was repeated for 2 weeks. Four weeks after transplantation, [18F]FP-CIT PET and the rotation test were repeated. All 6-OHDA-injected mice showed markedly decreased [18F]FP-CIT uptake in the right striatum. Transplanted F3-effLuc cells were visualized on the right side of the brain in all mice by bioluminescence imaging. The bioluminescence intensity of the transplanted F3-effLuc cells gradually decreased until it was undetectable by 10 days. The behavioral test showed that stem cell transplantation attenuated the motor symptoms of PD. No significant change was found in [18F]FP-CIT imaging after cell transplantation. We successfully established an in vivo bioluminescence imaging system for the detection of transplanted NSCs in a mouse model of PD. NSC transplantation induced behavioral improvement in PD model mice.</P>

Anti-Inflammatory Activity of Bee Venom in BV2 Microglial Cells: Mediation of MyD88-Dependent NF- <i>κ</i> B Signaling Pathway

Im, Eun Ju,Kim, Su Jung,Hong, Seung Bok,Park, Jin-Kyu,Rhee, Man Hee Hindawi Publishing Corporation 2016 Evidence-based Complementary and Alternative Medic Vol.2016 No.-

<P>Bee venom has long been used as a traditional folk medicine in Korea. It has been reportedly used for the treatment of arthritis, cancer, and inflammation. Although its anti-inflammatory activity in lipopolysaccharide- (LPS-) stimulated inflammatory cells has been reported, the exact mechanism of its anti-inflammatory action has not been fully elucidated. Therefore, the aim of this study was to investigate the anti-inflammatory mechanism of bee venom in BV2 microglial cells. We first investigated whether NO production in LPS-activated BV2 cells was inhibited by bee venom, and further iNOS mRNA and protein expressions were determined. The mRNA and protein levels of proinflammatory cytokines were examined using semiquantitative RT-PCR and immunoblotting, respectively. Moreover, modulation of the transcription factor NF-<I>κ</I>B by bee venom was also investigated using a luciferase assay. LPS-induced NO production in BV2 microglial cells was significantly inhibited in a concentration-dependent manner upon pretreatment with bee venom. Bee venom markedly reduced the mRNA expression of COX-2, TNF-<I>α</I>, IL-1<I>β</I>, and IL-6 and suppressed LPS-induced activation of MyD88 and IRAK1 and phosphorylation of TAK1. Moreover, NF-<I>κ</I>B translocation by IKK<I>α</I>/<I>β</I> phosphorylation and subsequent I<I>κ</I>B-<I>α</I> degradation were also attenuated. Thus, collectively, these results indicate that bee venom exerts its anti-inflammatory activity via the IRAK1/TAK1/NF-<I>κ</I>B signaling pathway.</P>

Actinomycin D 가 白鼠 培養心筋細胞에 미치는 細胞毒性에 關한 硏究

任珍洙,金丁中,崔玟圭 圓光大學校 醫科學硏究所 1990 圓光醫科學 Vol.6 No.1-2

In an attempt to evaluate the cytotoxic effect of actinomycin D, neutral red (NR), tetrazolium MTT and protein contents were measured by colorimetric assay using myocardial cells of the newborn rat cultured for 96 hrs in media containing various concentrations of actinomycin D. Light and electron microscopic studies were also performed to reveal the fine structure in actinomycin D-treated myocardial cells. The results obtained were as follows : 1. NR_90 and NR_50 values were measured at 1×10 exp (-3) ㎍/㎖ and 6×10 exp (-2) ㎍/㎖ of actinomycin D, respectively, and MTT_90 and MTT_50 were also calculated to be 4×10 exp (-3) ㎍/㎖ and 7×10 exp (-2) ㎍/㎖ of actinomycin D. 2. Actinomycin D was highly toxic on cultured rat myocardial cells by both the NR_50 and MTT_50 values measured below the 0.12 ㎍/㎖ of actinomycin D, according to Borenfreund et al. 3. Protein contents of myocardial cells in the NR_50 and NTT_50 of actinomycin D were reduced to 24.3% and 21.7% of those of the control, respectively. 4. Light microscopy, showed both decrease in number of cells and weakness of beating in myocardial cells exposed to actinomycin D, time and dose-dependently. 5. In an electron microscopy, cisternal dilatation of rough endoplasmic reticulum, many vacuoles and loss of myofibrils in sarcoplasm were observed in actinomycin D-treated myocardial cells. These results suggest that actinomycin D inhibits in vitro growth of murine cardiac muscle cells by damaging the cell organelles.

Pilomatricomas associated with rubinstein-taybi syndrome

( Im-kyu Lee ),( Hak-jun Kim ),( Dae-lyong Ha ),( Jung-soo Lee ),( Kihyuk Shin ),( Won-ku Lee ),( Hoon-soo Kim ),( Hyun-chang Ko ),( Byungsoo Kim ),( Moon-bum Kim ),( Jin-hwa Son ) 대한피부과학회 2021 대한피부과학회 학술발표대회집 Vol.72 No.2

청주 산업단지 인근 주민의 휘발성 유기화합물 노출

임유진, 송명섭, 박태현, 조현규, 이준호, 김용대, 김헌 충북대학교 의학연구소 2020 忠北醫大學術誌 Vol.30 No.1

본문참고

Wilson disease diagnosed incidentally by targeted gene panel sequencing in a Korean boy with severe obesity

Im Minji,Song Ari,Kim Jiyeon,Kim Min-Sun,Lee Sae-Mi,Kim Mi Jin,Cho Sung Yoon,Jin Dong-Kyu 대한소아내분비학회 2022 Annals of Pediatirc Endocrinology & Metabolism Vol.27 No.3

Wilson disease (WD) is a relatively common genetic hepatic disease in children and is characterized by excessive copper accumulation, predominantly in the liver and brain. It is an autosomal recessive disease caused by an ATP7B mutation that causes brain degeneration and is potentially fatal if diagnosed late or untreated. In the early phase of WD, its initial presentation may include mild hepatic involvement. WD may be overlooked as a cause of liver disease due to severe obesity but should not be excluded from differential diagnosis. We report a case of WD with severe obesity and fatty liver diagnosed in the early phase by targeted gene panel sequencing and review the endocrine problems associated with WD. Early suspicion of WD is important for good prognosis.

Novel bile acid derivatives induce apoptosis via a p53-independent pathway in human breast carcinoma cells

Im, Eun-ok,Choi, Yung Hyun,Paik, Kee-Joo,Suh, Hongsuk,Jin, Youngeup,Kim, Kyu-Won,Yoo, Young Hyun,Kim, Nam Deuk 부산대학교 유전공학연구소 2001 분자생물학 연구보 Vol.17 No.-

We have compared the anti-proliferative effects of ursodeoxycholic acid (UDCA), chenodeoxycholic acid (CDCA) and their derivatives, HS-1183, HS-1199 and HS-1200, on MCF-7 (wild-type p53) and MDA-MB-231 (mutant p53) cells. While UDCA and CDCA exhibited no significant effect, their novel derivatives inhibited the proliferation of both cell lines in a concentration-dependent manner, concomitant with apoptotic unclear changes and the increase of a sub-G1 population and DNA fragmentation. Furthermore, we also observed an increase in the ratio of pro-apoptotic protein Bax to anti-apoptotic protein Bcl-2 and cleavages of lamin B and poly(ADP-ribose) polymerase (PARP) in MCF-7 and MDA-MB-231 cells. Cell cycle related proteins, cyclin D1 and D3, as well as retinoblastoma protein (pRb) were down-regulated, while the level of cyclin-dependent kinase inhibitor p21^WAFI/CIPI was increased in both cancer cells after treatment with novel bile acids. These findings suggest that these cytotoxic effects of novel bile acid derivatives on human breast carcinoma cells were mediated via apoptosis through a p53-independent pathway. ⓒ 2001 Elsevier Science Ireland Ltd. All rights reserved.