참다래 수품종의 형태적 특성과 RAPD에 의한 유연관계 분석

조윤섭,조혜성,노일섭,김월수 전남대학교 농업과학기술연구소 2000 農業科學技術硏究 Vol.35 No.-

To examine the taxonomic relationships among 13 male kiwifruits by 36 morphological characters, principal component analysis and cluster analysis were carried out. Of 36 characters, hair density on ventral midribs and between ventral midribs, leaf length/leaf width ratio etc. were considered as very useful characters for the classification of Actinidiacea plants and number of white dots on branch, full flowering period and flower color etc. also showed significant differences among A. deliciosa, A. chinensis and wild kiwis. Results of cluster analysis using scores of the principal component indicated that 13 male kiwifruits could be grouped into A. deliciosa, A. chinensis and wild kiwis phenon at 0.5 of average distance in WMVCA. This result was also very similar to that of RAPD analysis. However, it seems that more characters including various kinds of Actinidia species are needed for the exact classification.

地溫이 고추葉의 生長과 發育에 미치는 影響

盧一燮,金鎭漢 충북대학교 농업과학기술연구소 1990 農業科學硏究 Vol.8 No.1

This study was conducted to examine the effects of rhizosphere temperature on the growth of seedlings, internal and external morphology of leaves and absorption of mineral nutrients in pepper (Capsicum annuum L). Pepper seedlings were grown at rhizosphere temperature of 13℃, 18℃, 23℃, 28℃ and 33℃, respectively. Leaf thickness, chlorophyl content, number of chloroplasts, number of cells, size and number of palisade cells and number of stomata were measured and inoganic eleme-nts were analized. The results were summarized as follows ; 1.Seedling weight and fruit weight were heavier at 23℃, while they were reduced greatly at 13℃. 2.Leaf length and leaf width were largest at 23℃ and 28℃, respectively and both traits were became smaller as temperatures were increased or decreased. 3.Chlorophy Ⅱ content and number of chloroplasts were highest at 18℃ 4.Surface area and volume of palisade cells were significally greater at 23℃ and considerably reduced at 13℃. 5.Number of stomata was highest at 13℃,while no significant difference were noted among other temperatures. Number of stomata was about 4 times higher at abaxial side than at adaxial side. 6.Contents of inoganic nutrient analyzed except phosphate, such as T- N, K2O, MgO and CaO, in leaf, stem and root were higher at 23℃ and tended to be decreased at higher or lower temperatures.

자동 저면권수장치의 개발과 그의 활용

김진한, 조삼증, 노일섭 忠北大學校 1986 論文集 Vol.32 No.-

-본문참고-

적심 및 분지(分枝) 유도를 위한 신규 생장조절제로서의 mevalocidin 활용

김진석(Jin-Seog Kim),연규환(Gyu Hwan Yon),김보관(Bo Gwan Kim),최정섭(Jung-Sup Choi),김은애(Eun Ae Kim),고영관(Young Kwan Ko),이일영(Ill Young Lee) 한국잡초학회·한국잔디학회 2020 Weed & Turfgrass Science Vol.9 No.1

현재 적심 및 분지조절제로서 여러 가지 약제가 개발되어 사용되고 있지만 화합물마다 약해, 효능 부족, 심한 잔류성, 좁은 사용범위, 기타 부작용 등의 단점이 있기 때문에 이를 보완할 수 있는 보다 안전하고 새로운 유형의 물질 탐색과 개발은 매우 필요한 상황이다. 본 연구에서는 미생물 유래의 천연물인 mevalocidin에 대해 적심제 및 분지생장조절제로서의 사용 가능성을 검토하였다. 토마토, 고추, 콩, 고구마, 들깨 유묘를 대상으로 여러 농도의 mevalocidin을 처리해 본 결과, 흡수된 mevalocidin은 정단부로 빠르게 이동하여 생장점을 고사시키는 특징을 보이며 상대적으로 체내 대사가 빠르게 진행되는 것 같았다. 작물에 따라 적정농도가 다르지만 전반적으로 2-4 kg ha<SUP>-1</SUP> 처리범위에서 양호한 적심효과가 나타났고, 1-3 kg ha<SUP>-1</SUP> 처리 범위에서는 정단 생장억제와 함께 분지가 유도되면서 유도된 분지의 양호한 생장이 진행되었다. 따라서 미생물 유래의 천연물인 mevalocidin은 원예작물, 식량작물, 일부 수목에 적심 및 분지(分枝) 유도를 위한 친환경적 신규 생장조절제로서 유용하게 활용될 수 있을 것으로 판단되었다. Modulating shoot architecture in many agricultural, horticultural, and ornamental industries, is important in increasing the yield and quality of their products. This study was conducted to investigate the possibility to use mevalocidin as a new plant growth regulator for thinning and lateral shoot induction. Mevalocidin which was discovered from static cultures of two fungal isolates by Dow Agrosciences, were sprayed with various rates at the seedlings of five crops (tomato, pepper, soybean, sweet potato, and perilla) and investigated several cultural traits such as shoot growth, lateral shoot induction and length. As a results, mevalocidin was seemed to rapidly move into shoot tip and selectively killed an apical meristem part or inhibit its growth at a proper dosage. Then the induced-lateral shoots were grown rapidly, showing that the chemical was easily metabolized in plant. Generally mevalocidin at 2-4 kg ha<SUP>-1</SUP> (500- 1,000 ppm) led effectively to kill the apical part although the proper rate was different to crop species. On the other hand, The lower rate of mevalocidin (1-3 kg ha<SUP>-1</SUP>, 250-750 ppm) were effective for height control and lateral branching through the break of apical dominance. Therefore, it seemed that mevalocidin, natural phytotoxin from microorganism metabolites, could be used as a new eco-friendly plant growth regulator for thinning and lateral shoot induction for agricultural, horticultural, and ornamental industries.

Mixed Rare Earth <tex> $({\rm Nd}_{1/3}{\rm Eu}_{1/3}{\rm Gd}_{1/3}){\rm Ba}_{2}{\rm Cu}_{3}{\rm O}_{7-{\rm d}}$</tex> Thin Films by PLD for Coated Conductor

Rock-Kil Ko,Sung-Hwan Bae,Myung-Jin Jung,Se-Hoon Jang,Kyu-Jeong Song,Chan Park,Myung-Hwan Sohn,Suk-ill Kang,Sang-Soo Oh,Dong-woo Ha,Hong-Soo Ha,Ho-Sup Kim,Young-Cheol Kim IEEE 2009 IEEE transactions on applied superconductivity Vol.19 No.3

<P>In order to investigate the possibility of using mixed rare earth (Nd<SUB>1/3</SUB>Eu<SUB>1/3</SUB>Gd<SUB>1/3</SUB>)Ba<SUB>2</SUB>Cu<SUB>3</SUB>O<SUB>7-x</SUB> (NEG123) as the superconducting layer of the HTS coated conductor, the NEG123 thin film was deposited epitaxially on LAO(100) single crystal and IBAD_YSZ metal templates by pulsed laser deposition. Systematic studies were carried out to investigate the influences of deposition parameters of PLD on the microstructure, texture and superconducting properties of NEG-123 coated conductor. Deposition at oxygen partial pressure of 600 mTorr was needed to routinely obtain high quality NEG123 films with J<SUB>C</SUB>'s (77 K) over 2 MA/cm<SUP>2</SUP> and Tc's over 90 K (DeltaT ~ 2 K) . We verified from magnetization study that the NEG123 has an improved in-field Jc as the field increases at temperatures between 10 K and 77 K compared with Gd123. The J<SUB>C</SUB> (77 K, self field) and the value of onset T<SUB>C</SUB> of NEG123 thin film on LAO substrate was 4.0 MA/cm<SUP>2</SUP> and 92 K, respectively. This is the first report, to the best of our knowledge, of coated conductors with NEG123 film as the superconducting layer which have Ic and Jc over 40 A/cm-width and 1.6 MA/cm<SUP>2</SUP> at 77 K, self field. This study shows the possibility of using NEG123 film as the superconducting layer of the HTS coated conductor which can be used in high magnetic field power electric devices.</P>

SEM observation the pollen-stigma interaction in self-incompatible strains of brassica campestris

Ill Sup Nou(盧一燮),Hyo Yeon Lee(李孝淵)Seon Ha Lee(李善河)Jin Ho Kim(金晉鎬)Kokichi Hinata(日向康吉) 한국육종학회 1994 한국육종학회지 Vol.26 No.2

The behavior of pollen and stigma was compared between self(incompatible)-and cross(compatible)-pollinations in connection with self-incompatibility in Brassica campestris L. through scanning electron microscope(SEM). Whithin 30 min after pollination, swollen pollen grains began to emerge a large quantity of secretion from one of the three furrows. The secretion covered the papilla cells and pollen altogether in the form of a film for about two hrs. The swelling was quicker and secretion was more abundant in the cross-pollination as compared with the self-pollinations. Recognition reaction between self and non-self occurs during the phase, but the discrimination doses not give conclusive effects. As the result of the recognition reaction, self-pollen tubes are unable to penetrate the papilla cell walls. Thus, the recognition reaction occurs at two phases ; pollen hydration-germination and pollen tube penetration. Although the self pollen tubes cannot penetrate papilla cells, the contact between a pollen papilla without breakage. This suggests that they were not separated from papilla without breakage. This suggests that a metabolic interaction has occurred between pollen tubes and papilla cells. On the other hand, the self pollen tubes elongated on the surface of papilla cells during night, indicating that they have a potential to elongate after the contact.

同形化型 自家不和合性에 관한 硏究動向 Ⅱ. 配偶體型 自家不和合性에 관한 遺傳分析 및 分子的 硏究

Ill Sup Nou(盧一燮),Hyo Yeon Lee(李孝淵),Jin Ho Kim(金晋鎬) 한국육종학회 1993 한국육종학회지 Vol.25 No.1

In gametophytic self-incompatibility, pollen tubes expressing S-alleles identical to those expressed in the style cease growth in the upper third of the style. The S-allele products of pollen and discriminate between self and cross-pollen. S-allele-associated pistil proteins(S-glycoproteins) have been identified in many Solanaceae. The sequences of a number of Solanaceous S-glycoprotein have been deduced from the corressponding cDNA sequences and compared their primary structure between them. The biological function of S-glycoproteins in the self-incompatibility interactions has not been demonstrated. S-glycoproteins corresponding to the most S-alleles isolated from style extracts of Nicotiana, Petunia, Solanum has a ribonuclease(RNase) activity. But the arrest of pollen tube growth was not simply explained by RNase action, and transcription and glycosylation process is necessary for the expression of self-incompatibility, plural genes or action sites may be involved in an S-gene. There are some unresolved question in RNase hypothesis ; How can be explain S-allele specificity? What is substrate of RNase? What is self-incompatibility related substance in pollen side against style side? Anyhow, at the moment, differences between gametophytic and sporophytic systems are clear, and we are inevitably believe that plants having different structure of flower have obtained respective systems to secure the genetic heteroveneity in the course of evolution.

Genome-wide Identification of Cold and Freezing Stress Responsive Genes of Brassica oleracea

Ill-Sup Nou,Mi-Young Chung,Hye-Ran Kim,Hee-Jeong Jung,Jong-In Park,Nasar Uddin Ahmed 한국원예학회 2013 한국원예학회 학술발표요지 Vol.2013 No.10

Brassica campestris로부터 自家不和合性 관련 SLG⁴⁸ cDNA의 cloning 및 sequencing

Ill Sup Nou(盧一燮),Hak Soon Choi(崔鶴淳),Hyo Yeon Lee(李孝淵) 한국육종학회 1996 한국육종학회지 Vol.28 No.1

A cDNA sequence homologous to the Brassica self-incompatibility locus specific glycoprotein sequence was isolated from stigmas of B. campestris plants homozygous for the SLG⁴⁸-allele. The relative molecular mass and isoelectric focusing value were calculated to be 50~60KD and pI 9.5, respectively. SLG⁴⁸ cDNA library consisting of 2.5×10⁵ recombinant clones was obtained from the poly (A)⁺ RNA. The cDNA were ligated to λZAPII arms. The selected cDNA clone was subcloned by in vivo excision using the helper phage R408 and E. coli (JM109). The resultant recombinant plasmid was sequenced with Sequenase Ver 2.0 and a deletion kit. The cDNA contains on open reading frame of 1137bases (379 amino acids), a non-coding 3’ region and a poly (A) tail, six N-glycosylation site. Overall the SLG⁴⁸ and SLG⁴⁵ or SRA⁰¹ sequences showed 80.4%, or 53.3% homology at the amino acid level. The SLG⁴⁸ sequence includes 12 cysteine residues in the carboxyl half of the molecule which are conserved with respect of the SLG⁴⁵ and SRA⁰¹. It is indicates that this common characterization of cysteine residues does not specify the self-incompatibility response.

Genome Wide Identification and Expression Analysis of the WRKY Gene Family in Brassica rapa

Ill-Sup Nou,Mi-Young Chung,Yong-Gu Cho,Hye-Ran Kim,Hee-Jeong Jung,Jong-In Park,Md. Abdul Kayum 한국원예학회 2013 한국원예학회 학술발표요지 Vol.2013 No.10