http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Hyunjoo Hwang,Yangjoo Kang,Kibum Kim,Kisung Ko 한국당과학회 2022 한국당과학회 학술대회 Vol.2022 No.07
Acute respiratory syndrome coronavirus SARS-CoV-2 causing COVID-19 has been rapidly spreading worldwide since it occurred in December 2019. SARS-CoV-2 infection occurs as the receptor-binding domain (RBD) of the coronavirus envelope spike protein binds to the angiotensin-converting enzyme 2 (ACE2) receptor. ACE2 is a cell membrane protein activated when it is cleaved and released from the cell surface. The soluble ACE2 can act as a decoy receptor to neutralize the SARS-CoV-2 virus, which is a potential therapy for COVID-19. In this study, the recombinant ACE2 protein was produced in a transient Nicotiana benthamiana plant expression system. The ACE2 was cloned to a fragment crystallizable (Fc) tagged with the KDEL sequence, endoplasmic reticulum (ER) retention signal (ACE2-FcK) in pEAQ-HT, the Cowpea mosaic virus (CPMV)-based transient plant expression vector. To design the recombinant protein structure of ACE2-FcK, the transmembrane region of ACE2 was removed using a transmembrane region prediction program. Four N-glycosylation sites recognized were identified using the N-glycosylation prediction program. Finally, Agrobacterium (LBA4404) carrying pEAQ-HT ACE2-FcK vector was applied for transient expression of ACE2-FcK in N. benthamiana. The proteins (100kDa) were successfully expressed and purified from leaves using protein A affinity chromatography. Additionally, suggested that the plant-derived ACE2-FcK has bioactivities as a therapeutic agent for coronavirus. Taken together, the ACE2-FcK protein can be transiently produced with an optimized expression time and leaf position post-infiltration in plant.
Hwang, Eunson,Sun, Zheng-Wang,Lee, Taek Hwan,Shin, Heon-Sub,Park, Sang-Yong,Lee, Don-Gil,Cho, Byung-Goo,Sohn, Hyunjoo,Kwon, Oh Wook,Kim, Sun Yeou,Yi, Tae Hoo The Korean Society of Ginseng 2013 Journal of Ginseng Research Vol.37 No.4
UV irradiation is the main factor contributing to skin damages that are associated with an excessive production of matrix-degrading metalloproteinase (MMP)-1 and a deficient expression of collagens. To date, red ginseng has been revealed to possess many biomedical effects, such as anti-aging, anti-oxidation, and anti-inflammatory. In this study, we prepared the Korean Red Ginseng extracts treated with enzyme (KRGE) and investigated the effects of dietary KRGE on the formation of wrinkles generated by UVB irradiation in hairless mice. It was found that KRGE inhibited the UVB-induced formation of wrinkles, epidermal thickness, and skin dryness in hairless mice. Further results also showed that KRGE attenuated UVB-induced MMP-${\beta}$1 level, while accelerated procollagen type I, transforming growth factor-${\beta}$1 secretion. Interestingly, the expression of profilaggrin and filaggrin in both the epidermis and dermis were decreased due to UVB exposure and reversed by KRGE. The KRGE 0.06% was prior to KRGE 0.24%. In view of these results, which indicated that KRGE protected skin from UVB-induced photodamages, which may not only mediated by regulating of MMP-1 and procollagen type I, but also by increasing the production of profilaggrin and filaggrin. In conclusion, our results suggest that KRGE may be a promising agent for the treatment of skin photodamages. The challenge of KRGE will be expected as cosmeceuticals and nutraceuticals in order to intervene in aging-related degenerative skin changes.
Facile and scalable synthesis of SiO<sub>x</sub> materials for Li-ion negative electrodes
Hwang, Junesun,Kim, Kyungbae,Jung, Woo-Sang,Choi, Hyunjoo,Kim, Jae-Hun Elsevier 2019 Journal of Power Sources Vol.436 No.-
<P><B>Abstract</B></P> <P>Amorphous SiO materials are considered to be alternative high-capacity anode materials for Li-ion batteries. However, the conventional preparation method is a high-temperature and energy-consuming vacuum process. In this study, a simple strategy to produce a SiO<SUB>x</SUB> material is proposed. Low-cost micron-sized Si and amorphous SiO<SUB>2</SUB> powders are mechanochemically mixed using a high-energy mechanical milling process. As a result, SiO<SUB>x</SUB> materials are produced that have the potential to replace the current commercial SiO powders. The X-ray photoelectron spectra exhibit that the chemical bonding state of Si in the synthesized SiO<SUB>x</SUB> materials is similar to commercial SiO. The electron microscopic analyses demonstrate that the secondary particle sizes are reduced and Si crystallites with sizes of a few nanometers to a few tens of nanometers are embedded in the silicon oxide matrix. Due to the morphology and microstructure, the initial coulombic efficiency and cycling stability of the SiO<SUB>x</SUB> materials are greatly improved compared to the commercial SiO sample. To further enhance the electrochemical properties, carbon incorporations are carried out, which result in an excellent cycle performance being achieved for the Li-ion battery anodes. The ball-milled SiO<SUB>x</SUB> material has ample potential as an alternative to conventional SiO materials.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A simple strategy to produce a SiO<SUB>x</SUB> material by ball-milling is proposed. </LI> <LI> The chemical mixing of Si and SiO<SUB>2</SUB> powders could be achieved. </LI> <LI> The electrochemical properties of the SiO<SUB>x</SUB> materials were greatly improved. </LI> <LI> The synthesized SiO<SUB>x</SUB> materials have the potential to replace the commercial SiO. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Eunson Hwang,Zheng-wang Sun,Taek Hwan Lee,Heon-Sub Shin,Sang-Yong Park,Don-Gil Lee,Byung-Goo Cho,Hyunjoo Sohn,Oh Wook Kwon,Sun Yeou Kim,Tae Hoo Yi 고려인삼학회 2013 Journal of Ginseng Research Vol.37 No.4
UV irradiation is the main factor contributing to skin damages that are associated with an excessive production of matrixdegrading metalloproteinase (MMP)-1 and a deficient expression of collagens. To date, red ginseng has been revealed to possess many biomedical effects, such as anti-aging, anti-oxidation, and anti-inflammatory. In this study, we prepared the Korean Red Ginseng extracts treated with enzyme (KRGE) and investigated the effects of dietary KRGE on the formation of wrinkles generated by UVB irradiation in hairless mice. It was found that KRGE inhibited the UVB-induced formation of wrinkles, epidermal thickness, and skin dryness in hairless mice. Further results also showed that KRGE attenuated UVB-induced MMP-1 level, while accelerated procollagen type I, transforming growth factor-β1 secretion. Interestingly, the expression of profilaggrin and filaggrin in both the epidermis and dermis were decreased due to UVB exposure and reversed by KRGE. The KRGE 0.06% was prior to KRGE 0.24%. In view of these results, which indicated that KRGE protected skin from UVB-induced photodamages, which may not only mediated by regulating of MMP-1 and procollagen type I, but also by increasing the production of profilaggrin and filaggrin. In conclusion, our results suggest that KRGE may be a promising agent for the treatment of skin photodamages. The challenge of KRGE will be expected as cosmeceuticals and nutraceuticals in order to intervene in aging-related degenerative skin changes.