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Koo, Minseon,Cho, Ae-Ri,Jeong, A-Ram,Kim, Hyun Jung,Park, Yong-Ho,Kwak, Hyo-Sun,Hwang, In-Gyun The Korean Society for Applied Biological Chemistr 2013 Applied Biological Chemistry (Appl Biol Chem) Vol.56 No.3
Enterococci have been used as starter cultures and probiotics. They also have been considered as indicator organisms for antibiotic resistance due to their ability to harbor and to easily acquire antibiotic resistance. This study aimed to show the antimicrobial resistance profiles and genotyping of Enterococcus faecalis in retail pork meat products in Korea. Enterococcus spp. were analyzed for 124 collected samples, which included minced pork meat, marinated pork meat with soy sauce or kochujang (fermented hot pepper-soybean paste), and frozen processed pork meat products. The isolates of E. faecalis (n =36) were resistant to tetracycline (58.3%), erythromycin (11.1%), and nitrofurantoin (2.8%). No vancomycin resistant enterococci were observed in the present study. Most of the E. faecalis isolates were sensitive to all antibiotics or resistant to single antibiotics. As a result of the automated repetitive-sequence-based PCR (rep-PCR), which was used as an approach for genotyping enterococci, 7 out of 36 isolates of E. faecalis were assigned to one cluster with a similarity >95%, and all isolates were found to have originated from minced pork meat, suggesting that this clone might circulate in minced pork meat products. Given the importance of antimicrobial resistance of enterococci in food safety as well as in public health, our results on the occurrence, antimicrobial resistance, and genotyping could provide useful information to derive risk management options.
Contamination Patterns and Molecular Typing of Bacillus cereus in Red Pepper Powder Processing
오세욱,Minseon Koo,Hyun Jung Kim 한국응용생명화학회 2012 Applied Biological Chemistry (Appl Biol Chem) Vol.55 No.1
Prevalence of Bacillus cereus was determined in red pepper powder samples obtained from various processing steps (n=60), swabs of machinery surfaces (n=35), and air samples of the processing room (n=17) during red pepper powder production. The detection rate of B. cereus was high (over 85%) in samples of raw materials and washing steps and decreased to 40% in samples obtained from later stages of processing, i.e. milling, metal detection, and final products. B. cereus was detected in 2.9 and 11.8% of swabs and air samples, respectively. The genetic similarity of B. cereus isolates obtained from various processing steps was compared to identify the sources of contamination for red pepper powder using the repetitive-sequence-based polymerase chain reaction method. For 20 isolates of B. cereus from two independent samplings, 50% of the isolates, which were clustered,consisted of two or more isolates with a similarity greater than 95%. For the isolates obtained from raw materials, crude milling,and milling step of the independent sampling, high genetic similarity (>99.4%) was observed, suggesting that these isolates may have originated from the same clone. The prevalence of B. cereus and molecular typing result suggest possible routes of contamination, such as the transfer of clone contaminated in raw materials to the red pepper powder and cross contamination during processing. Based on this result, more intervention studies to prevent pathogen contamination during red pepper powder processing are needed to insure good hygienic level of products.
해외 학술활동 - International ICFMH Symposium Food Micro 2012 학회 참관 및 터키 발효식품 조사
구민선,Koo, Minseon 한국식품연구원 2012 食品技術 Vol.25 No.3
본 내용은 터키 이스탄불에서 2012년 9월 3일부터 7일까지 개최된 "International ICFMH Symposium Food Micro 2012"에서 발표한 연구결과와 현지에서 수집한 발효식품의 최신 연구 동향/정보로서, 된장의 발효 미생물 선정 및 산업화 공정 개발 과제 수행에 활용하고자 한다.
김민선(Minseon Kim),조린(Lin Cho),임수민(Sumin Lim),Li Bingxi,구명완(Myoungwan Koo) 한국방송·미디어공학회 2022 한국방송공학회 학술발표대회 논문집 Vol.2022 No.11
본 논문에서는 네일 아트를 한 손 이미지가 주어졌을 때 손톱에 있는 네일 아트의 컬러를 자동으로 분류해주기 위한 시스템을 제안한다. 네일 아트 컬러 자동 분류기는 Object Detection 모델을 이용하여 인풋으로 들어오는 손 이미지에서 손톱 영역을 찾고, 각 손톱에 대하여 13 가지 컬러 중 하나로 분류한 결과를 아웃풋으로 반환한다. 본 프로젝트에서는 사용자가 요청하는 네일 아트 손 이미지에 대하여 컬러 라벨링 결과를 반환해주는 API 형태의 서비스를 제안하며, 반응형 웹을 통해 시연 가능하도록 시스템을 설계 및 구현하였다.
Kim, Hyun Jung,Koo, Minseon,Jeong, A-Ram,Baek, Seung-Youb,Cho, Joon-Il,Lee, Soon-Ho,Hwang, In-Gyun The Korean Society for Applied Biological Chemistr 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.3
Pathogenic E. coli is a major foodborne pathogen associated with gastroenteritis worldwide. Fresh vegetable products as well as raw meat and meat products have been recognized as important modes of transmission within the foodborne route. The objective of the present study was to determine the presence of six virulence factors ($stx_1$, $stx_2$, lt, st, eaeA, and ial) in E. coli isolated from fresh vegetable products to provide information on risk assessment of pathogenic E. coli in Korea. From 416 collected samples, including vegetable salad mix, sprouts, baby leaf vegetables, and unpasteurized fruit and vegetable juices commercially available in Korea, a total of 30 samples were positive for E. coli strains, resulting in an overall prevalence of 7.2%. Of the 120 E. coli isolates, only one isolate (0.8%), which was obtained from unpasteurized fruit and vegetable juices, was confirmed to possess the eaeA gene, but lacked stx genes. This study showed that some fresh vegetable product samples were contaminated with enteropathogenic E. coli.
Ko, Seunghwan,Lee, Junsu,Koo, Joonhoi,Joo, Beom Soo,Gu, Minseon,Lee, Ju Han IEEE 2016 Journal of Lightwave Technology Vol.34 No.16
<P>We propose a chemical wet etching technique that does not make use of toxic Hydrofluoric solution to produce cladding-etched optical fiber intended for use as a platform for a fiberized saturable absorber. The wet etching technique is based on the use of a mixed solution of NH4F and (NH4)(2)SO4, which is not toxic, and a small, specially-devised etching cradle to achieve precise control of the etched fiber length. A fiberized saturable absorber was implemented by depositing the graphene oxide particles on top of the residual cladding for our cladding-etched fiber by using the drop & dry method. The saturable absorber was incorporated into an erbium-doped fiber ring cavity to test its suitability as a mode-locker with a modulation depth larger than 4.3%. Femtosecond pulses with a temporal width of similar to 615 fs were readily obtained at a repetition rate of similar to 17.09 MHz. The saturable absorption and laser performance of our saturable absorber were compared to those of an absorber on a core-etched fiber platform prepared through the formation of microdroplets of HF and surface tension-driven flow, as in [57].</P>