마이크로 프레스 구동을 위한 기어시스템 개발

홍용,김현식,고유곤,홍동표,김성욱 한국공작기계학회 2008 한국공작기계학회 추계학술대회논문집 Vol.2008 No.-

In this paper, gear system for Micro-press actuator parts is developed. The micro-press system consists of AC geared servo motor, gear system and high precision ball screw. Herein, the micro-press gear system not only forms the micro thin foil valve but also forms the hydro dynamic bearing for a high precision rotational equipment. we analyzed the transmitted power capacity of gear system and torque, angular velocity of ball screw, because we are to select the appropriate servo motor and then we apply result of analysis to the micro press system. For the next step, we plan to study for the development of forming speed for a productivity with high precision.

마이크로 프레스 구동을 위한 기어시스템 개발

홍용,김현식,고유곤,홍동표,김성욱 한국공작기계학회 2008 한국공작기계학회 춘계학술대회논문집 Vol.2008 No.-

In this paper, gear system for Micro-press actuator parts is developed. The micro-press system consists of AC geared servo motor, gear system and high precision ball screw. Herein, the micro-press gear system not only forms the micro thin foil valve but also forms the hydro dynamic bearing for a high precision rotational equipment, we analyzed the transmitted power capacity of gear system and torque, angular velocity of ball screw, because we are to select the appropriate servo motor and then we apply result of analysis to the micro press system. For the next step, we plan to study for the development of forming speed for a productivity with high precision.

培養鷄胚筋細胞分化에 미치는 細胞融合促進物質의 영향

朴映淳,朴鍾君,金賢玉,金鍾鎬,李東燁,金完柱 圓光大學校 1988 論文集 Vol.22 No.2

培養鷄胚筋細胸 分化에 미치는 促進物質의 영향을 알아보고자 2가양이온 (Ca2+, Mg2+) 胸胚抽出物 (EE), polyethylene glycol (PEG), 再生中인 도마뱀 (Lacertilia)꼬리 추출물 (BE)을 처리하여 細胸融合指數를 비교 조사하였다. 정상배양시의 細胸融合指數는 배양시작 70 시간에 최대에 달했으며 더이상 증가하지 않았고, Ca2+와 Mg2+ 처리할 경우에는 농도가 증가할수록 細胸融合指數는 저하되고, 근세포의 굵기도 정상세포보다 가늘고 핵이 모여있었다. Polyethylene Glycol (PEG)와 Blastemata Extract (BE) 처리군에서는 대조구와 큰 차이가 없는 것으로 나타났다. 鷄胚抽出物 (EE) 을 筋細胞分化에 필수적이나 과량으로 添加시에는 細胞融合은 억제되고 세포수는 증가되는것으로 나타났다. To study the effects of promoting factors on the differentiation of muscle cells, chick embryonic myoblasts were cultured with divalent cations (Ca2+, Mg2+) Chick embryo extract (EE), Polyethylene Glycol (PEG) and Blastema extract of Lacertilia (BE). Fusion index and Growth ratio were measured at various culture time. The fusion index of normal myoblast culture was reached maximum at 70 hours and the divalent cations (Ca2+, Mg2+) treated groups were decreased at higher concentration. The cellular formation of myoblasts treated with divalent cations was thiner and more aggregated between nuclei than that of normal myoblasts calture was. When polyethylene glycol (PEG) and Blastema extract of Lacertilia (BE) were treated to the myoblasts, tha fusion indices were similar to the control. Embryo Extract (EE) was seemed to be necessary for the fusion of chick embryonic myoblasts in culture, however, when excess of EE was added the fusion index was decreased and the number of cells was increased.

Association between coronavirus disease 2019-related workplace interventions and prevalence of depression and anxiety

Hyun Woo Park,Seung Hyun Park,Young Wook Kim,JunSeok Son,Chan Woo Kim,Hyoung Ouk Park,Jun Ho Lee,Young Hoo Shin,Chang Ho Chae,Eui Yup Chung,Hun Jeong 대한직업환경의학회 2022 대한직업환경의학회지 Vol.34 No.-

Surface Nanopatterning: Mussel‐Inspired Block Copolymer Lithography for Low Surface Energy Materials of Teflon, Graphene, and Gold (Adv. Mater. 47/2011)

Kim, Bong Hoon,Lee, Duck Hyun,Kim, Ju Young,Shin, Dong Ok,Jeong, Hu Young,Hong, Seonki,Yun, Je Moon,Koo, Chong Min,Lee, Haeshin,Kim, Sang Ouk WILEY‐VCH Verlag 2011 Advanced Materials Vol.23 No.47

<P>Mussel‐inspired block copolymer lithography is reported on page 5618 by Haeshin Lee, Sang Ouk Kim, and co‐workers. Mussel‐inspired poly‐dopamine coating enables the organic surface energy modification of low surface energy materials, such as Teflon, graphene, and gold, which is required for block copolymer lithography. Further pattern transfer generates unconventional nano structures, such as Teflon nanowires, nano‐patterned graphene, and vertical carbon nanotubes directly grown on Au substrates. The image shows an uncoated (left) and a polydopamine‐coated (right) cherry tomato. </P>

A Biodegradable Polymersome Containing Bcl‐xL siRNA and Doxorubicin as a Dual Delivery Vehicle for a Synergistic Anticancer Effect

Kim, Hyun‐,Ouk,Kim, Eunjung,An, Yonghee,Choi, Jihye,Jang, Eunji,Choi, Eun Bi,Kukreja, Aastha,Kim, Myeong‐,Hoon,Kang, Byunghoon,Kim, Dong‐,Joo,Suh, Jin‐,Suck,Huh, Yong‐,Mi WILEY‐VCH Verlag 2013 Macromolecular bioscience Vol.13 No.6

<P><B>Abstract</B></P><P>Combined cancer treatment via co‐delivery of siRNAs and an anticancer drug can be a promising strategy due to the synergistic effect of simultaneously minimizing gene/drug administration. In this study, Bcl‐xL siRNA and doxorubicin (DOX) are encapsulated into designed methoxy‐poly(ethylene glycol)‐<I>block</I>‐poly(<SMALL>D</SMALL>,<SMALL>L</SMALL>‐lactic acid) (mPEG‐<I>b</I>‐PLA) block copolymer polymersomes (PSomes). A study of the cytotoxicity of Bcl‐xL siRNA and DOX co‐encapsulated PSomes (CPSomes) shows more inhibited proliferation of MKN‐45 and MKN‐28 human gastric cancer cell lines than only gene‐ and drug‐loaded ones. Consequently, these results demonstrate that co‐delivery of genes and drugs using PSomes results in a synergistic efficacy and indicates the potential of PSomes as efficient nanocarriers for combined cancer therapy. </P>

점증부하 최대운동이 운동 전 후 혈중지질에 미치는 영향

김성수,현정화,신말순,김영표,이삼준,권순욱,이재현,김상호 대한스포츠의학회 1996 대한스포츠의학회지 Vol.14 No.1

This study was designed to investigate the effects of graded maximal exercises on serum lipids. The subjects consisted of 7 athletes group(A group) and 7 non-athlete group(B group) and who all were about twenty-one years old. Their blood samples were collected at pre and post(after exhaustion). The collected blood were analyzed for the triglyceride, cholesterol, High density lipoprotein cholesterol and TC/HDL-C ratio, Apo A, B. The result through the statistical analysis of this data were summarized as follows; 1. Triglyceride, total cholesterol, LDL-C, TC/HDL-C ratio level, athlete groups were higher than those in non-athlete groups after maximal exercise. There were no significant differences between two groups. 2. HDL-C level was decreased after maximal exercise in athlete groups but was increased in non-athlete groups. There were significant differences between two groups. 3. Apo A level was increased after maximal exercise in two groups, especially athlete groups were higher than those in non-athlete groups after maximal exercise. There were significant differences between two groups. 4. Apo B were no significant difference between two groups

Anomalous Rapid Defect Annihilation in Self-Assembled Nanopatterns by Defect Melting

Kim, Bong Hoon,Park, So Jung,Jin, Hyeong Min,Kim, Ju Young,Son, Seung-Woo,Kim, Myung-Hyun,Koo, Chong Min,Shin, Jonghwa,Kim, Jaeup U.,Kim, Sang Ouk American Chemical Society 2015 NANO LETTERS Vol.15 No.2

<P>Molecular self-assembly commonly suffers from dense structural defect formation. Spontaneous defect annihilation in block copolymer (BCP) self-assembly is particularly retarded due to significant energy barrier for polymer chain diffusion and structural reorganization. Here we present localized defect melting induced by blending short neutral random copolymer chain as an unusual method to promote the defect annihilation in BCP self-assembled nanopatterns. Chemically neutral short random copolymer chains blended with BCPs are specifically localized and induce local disordered states at structural defect sites in the self-assembled nanopatterns. Such localized “defect melting” relieves the energy penalty for polymer diffusion and morphology reorganization such that spontaneous defect annihilation by mutual coupling is anomalously accelerated upon thermal annealing. Interestingly, neutral random copolymer chain blending also causes morphology-healing self-assembly behavior that can generate large-area highly ordered 10 nm scale nanopattern even upon poorly defined defective prepatterns. Underlying mechanisms of the unusual experimental findings are thoroughly investigated by three-dimensional self-consistent field theory calculation.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/nalefd/2015/nalefd.2015.15.issue-2/nl5042935/production/images/medium/nl-2014-042935_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nl5042935'>ACS Electronic Supporting Info</A></P>

Molecular Insights Into the Evolutionary Pathway of <i>Vibrio cholerae</i> O1 Atypical El Tor Variants

Kim, Eun Jin,Lee, Dokyung,Moon, Se Hoon,Lee, Chan Hee,Kim, Sang Jun,Lee, Jae Hyun,Kim, Jae Ouk,Song, Manki,Das, Bhabatosh,Clemens, John D.,Pape, Jean William,Nair, G. Balakrish,Kim, Dong Wook Public Library of Science 2014 PLoS pathogens Vol.10 No.9

<▼1><P>Pandemic <I>V. cholerae</I> strains in the O1 serogroup have 2 biotypes: classical and El Tor. The classical biotype strains of the sixth pandemic, which encode the classical type cholera toxin (CT), have been replaced by El Tor biotype strains of the seventh pandemic. The prototype El Tor strains that produce biotype-specific cholera toxin are being replaced by atypical El Tor variants that harbor classical cholera toxin. Atypical El Tor strains are categorized into 2 groups, Wave 2 and Wave 3 strains, based on genomic variations and the CTX phage that they harbor. Whole-genome analysis of <I>V. cholerae</I> strains in the seventh cholera pandemic has demonstrated gradual changes in the genome of prototype and atypical El Tor strains, indicating that atypical strains arose from the prototype strains by replacing the CTX phages. We examined the molecular mechanisms that effected the emergence of El Tor strains with classical cholera toxin-carrying phage. We isolated an intermediary <I>V. cholerae</I> strain that carried two different CTX phages that encode El Tor and classical cholera toxin, respectively. We show here that the intermediary strain can be converted into various Wave 2 strains and can act as the source of the novel mosaic CTX phages. These results imply that the Wave 2 and Wave 3 strains may have been generated from such intermediary strains in nature. Prototype El Tor strains can become Wave 3 strains by excision of CTX-1 and re-equipping with the new CTX phages. Our data suggest that inter-chromosomal recombination between 2 types of CTX phages is possible when a host bacterial cell is infected by multiple CTX phages. Our study also provides molecular insights into population changes in <I>V. cholerae</I> in the absence of significant changes to the genome but by replacement of the CTX prophage that they harbor.</P></▼1><▼2><P><B>Author Summary</B></P><P>In this report, we suggest a genetic mechanism of how the <I>V. cholerae</I> atypical El Tor variants were generated from classical and prototype El Tor biotype strains. An intermediary strain, containing the CTX-1 and CTX-2 prophages, was identified among the clinical isolates that were collected in 1991, when the atypical strains emerged. This strain can be converted into various Wave 2 atypical El Tor strains by eliminating prototype components, CTX-1 and RS1. Further, new types of the CTX phage genome can be generated from the intermediary strain by inter-chromosomal recombination between CTX phages and recombination between the CTX phage and RS1. These new CTX phages can be transduced into other El Tor strains, transforming them into Wave 3 atypical strains. This is a demonstrated instance of how a single-segment-genome CTX phage re-organizes its genome through recombination between different types of phage, leading to generation of new phage variants and atypical El Tor strains.</P></▼2>

Dengue virus-Polymersome Hybrid nanovesicles for Real-Time Single Virus Tracking

( Hyun-ouk Kim ),( Woonsung Na ),( Minjoo Yeom ),( Seungjoo Haam ),( Daesub Song ) 대한인수공통전염병학회 2019 창립총회 및 학술대회 초록집 Vol.2019 No.1

Introduction: Dengue virus is one of the major infectious human pathogens worldwide. Currently, no antiviral drug has become available against the dengue virus induced diseases since little is known regarding how dengue virus interacts with host cells. Dengue virus-polymersome hybrid nanovesicles are powerful tools to explain the dynamics of host cell-virus interaction and tracking. Methods: Dengue virus-polymersome hybrid nanovesicles were prepared by dialysis method. We showed that DiD and BODIPY FL C5-Ceramide encapsulated dengue virus-polymersome hybrid nanovesicles triggered the formation of red and green fluorescence by using real-time fluorescence microscopy. The internalization to escape endosomal entrapment was determined by confocal laser scanning microscopy. Results: Fluorescence images of DiD and BODIPY FL C5-Ceramide encapsulated dengue virus-polymersome hybrid nanovesicles illustrated at different time points. For real-time virus tracking study, it is desirable to acquire images to obtain adequately high resolution to monitor the dynamics of host cell-virus interaction and tracking in living cells. Conclusion: This study exploited a virus-nanovesicles tracking technology to approach whether dengue virus interacts with autophagy. Therefore, we demonstrated that dengue virus-polymersome hybrid nanovesicles will be utilized for virus tracking studies to examine the mechanisms of viral infections.