Bacillus lentimorbus WJ5의 감마선유도 돌연변이체들에서 공통으로 발현되는 방사선 관련 유전자의 microarray 분석

이영근,장화형,장유신,허재호,형석원,정혜영 한국환경생물학회 2004 환경생물 : 환경생물학회지 Vol.22 No.3

본 연구에서는 감마선으로 유도된 돌연변이체들에서 공통으로 발현되는 방사선 관련 유전자들의 발현을 연구하기 위하여, B. lentimorbus WJ5의 방사선 유도 돌연변이체에서 발현되는 유전자를 DNA microarray로 동시에 탐색하였다. DNA microarray는 B. lentimorbus WJ5 genome을 무작위로 절단하여 2,000단편으로 구성하였으며, 감마선 (^(60)Co)으로 유도된 7돌연변이체의 발현을 정량적으로 관찰하였다. 클러스터 분석결과 발현된 408 유전자 중 27개가 감마선 유도 돌연변이체 모두에서 유의하게 발현이 증가되었다. 특히, 복구(mutL, mutM)에너지 대사 (acsA, sdhB, p하, yhjB, citB), protease(npr), 산화자극에 대한 환원 (HMM)관련 유전자들이 동시에 증가되었다. 이는 감마선 유도 돌연변이체들에서 자발적인 직/간접 복구 관련 유전자의 발현 증가는 방사선 노출 직후 보이는 stress response와는 다른 현상임을 나타내는 것으로 생각된다. To study the radiation related gene expression in mutants of Bacillus lentimorbus WJ5 induced by gamm radiation, the simultaneous gene expression was analyzed by DNA microarray. We constructed DNA chips including two thousand randomly digested genome spots of B. lentimorbus WJ5 and compared its quantitative aspect with seven mutants induced by gamma radiation (^(60)Co). From the cluster analysis of gene expression pattern, totally 408 genes were expressed and 27 genes were significantly upregulated by the gamma radiation in all mutants. Especially, genes involved in repair (mutL, mutM), energy metabolism (acsA, sdhB, pgk, yhjB, citB), protease (npr), and reduction response to oxidative stress (HMM) were simultaneously upregulated. It seems that the induction of the direct and/or indirect repair related genes in mutants induced by gamma radiation could be remarkably different from the adaptive responses against acute exposure to radiation.

사람 Ferritin 유전자를 이용한 담배의 형질전환

이주안,김형석,임채완,김해영,박영두 경희대학교 생명자원과학연구원 2002 硏究論文集 Vol.22 No.-

사람 H-chain ferritin 유전자를 Agrobcterium-mediated transformation 방법으로 담배(Nicotiana tabaccum L)에 전이시켰다. 이를 위하여 사람 H-chain ferritin 유전자를 C_(S)VMV promotor와 NOS terminator를 운반하는 plant binary vector, pILTAB 357에 도입하였다. pILTAB 357를 포함하는 Agrobcterium tumefaciens LBA4404로 담배 잎 절편체를 감염시킨 후 형질전환 신초는 MS배지에 1 5 ㎎/L BA, 50 ㎎/L kanamycin 그리고 200 ㎎/L cefotaxim이 첨가된 1차 선발배지에서 유도하였다. 유도된 신초는 MS배지에 100 ㎎/L kanamycin과 200 ㎎/L cefotaxim이 포함된 2차 선발배지에서 뿌리를 유기하였다. 뿌리가 형성된 재분화 개체는 온실에서 재배하였으며 자가수분하여 종자를 수확하였다. 사람 H-chain ferritin유전자의 담배 genome내로의 전이여부를 H-chain ferritin 유전자내의 primer를 이용하여 PCR 방법으로 확인한 바 형질전환 개체에서 예상했던 560bp 단편을 볼 수 있었으나 형질전환을 시키지 않은 식물체에서는 PCR 산물을 확인할 수 없었다. Kanamycin 저항성 분석은 전이유전자의 후대로의 안정적인 유전을 보여 주었으며 저항성 종자들은 homozygous개체를 확보하기 위해 온실에서 재배 중에 있다. A human H-chain ferritin gene was introduced into tobacco (Nicotiana tabaccum L.) plants by Agrobcterium-mediated transformation. To introduce this gene, human ferritin gene was cloned into plant binary vector, pILTAB 357, horboring CsVMV promotor and nopalin synthase terminator (3' NOS). After infection with Agrobacterium tumefaciens LBA4404 containing pILTAB 357, transformed shoots were induced from first selection media (MS media + 1.5 ㎎/L BA + 50 ㎎/L kanamycin + 200 ㎎/L^(1) cefotaxim). Induced shoots were rooted in second selection media (MS media + 100 ㎎/L kanamycin + 200 ㎎/L cefotaxim). Rooted shoots were cultivated, selfed, and seeds were harvested in the green house. To confirm the transfer of the human H-chain ferritin gene in the genome of tobacco plants, PCR was conducted using specific primers of the H-chain femritin gene. A PCR fragment of 560 bp was obtained from each transformed plants, which is the predicted size of the H-cham ferritin gene but not from nontransformed plants. Kanamycin resistance assay showed that transgenes were stably inherited to next generation in all lines. These resistance seeds from each line were cultivated in the green house to obtain homozygous lines for further study.

교육인권과 스포츠권에 관한 연구

이형석 원광대학교 대학원 2007 論文集 Vol.38 No.-

The purpose of this study is to consider the 'right to sports' which has emerged as a basic right and to enhance the understanding on sports in the field of law. First, I established a concept of sports by surveying where the right to sports idea came from and considered the significance of right to sports from the international viewpoint of 'sports for all'. I also exemplified other countries legal cases. Next, I demonstrated right to sports as a constitutional one. I referred to the freedom of congregation and association, and other associated rights such as the right to education, happiness and life. According to the study, there are two types of legal characteristics: civil liberties and as a basic social right. That is to say, the characteristic of free from the intervention and invasion from the country and the right for individual to ask an authority to establish and implement policies for right to sports actualization.

체벌에 관한 연구

이형석 원광대학교 대학원 2007 論文集 Vol.39 No.-

The purport of education is to help students-as educational target-recognize and turn values. The real value of education lies in motivating students to achieve their desired goals for themselves with sound sense of value and intelligence. That is why we must correctly recognize and institutionalize teacher's educational authority and student's human rights before addressing the priority between the two rights. In view of the history of human rights, children and students were considered beneficiary of rights before their own rights were legitimately accepted in public, but they were not recognized as independent persons who can enjoy or claim their own rights. The Constitution of Republic of Korea assures human respect, values and personal rights. These are basic rights that everyone must enjoy, and our right derives from human respect, values and personal rights. Regardless of these essential aspects, our current educational sites commit corporal punishment in the name of 'whip of love', 'fair club' or the like, and such bodily punishment is actually understood in the context of standing and assurance of educational authority. In addition, according to feasible theory of educational authority or subrogation of parental authority, the rights of children or students are considered as a subordinary right subject to parental custody, and bodily punishment is indeed justified by the logics advocating educational authority substituted for this parental custody. The necessity and effects of corporal punishment have been reported in form of theory and findings associated with Law of Reinforcement from the perspective of pedagogy and psychology. Moreover, the behavioral practices of some teachers who blindly believe the effectiveness of such punishment also bring about many issues. Thus, it is required to make jurisprudential review of human rights and respect before addressing pedagogic efficiency of bodily punishment.

돼지 우리 깔집용 왕겨에서의 Ochratoxin A 검출

이문한,박진봉,박응복,이영재,신광순,박종명,남궁선,조준형,박근식,권택석,이재구 한국수의공중보건학회 1992 예방수의학회지 Vol.16 No.1

蔣敦復 詞論考

이석형 중앙대학교 외국어문학연구소 1999 외국학연구 Vol.- No.3

〈蕙風詞話〉'中拙大' 意味辨析

李奭炯 한국중국어문학회 2000 中國文學 Vol.33 No.-

重拙大是況周願蕙風詞話的論詞核心. 爲了明白況周願的詞論, 應先理解重拙大的意味. 一般辭典裏減不到重拙大這個詞語的名目或解釋. 按漢語大詞典拘解釋, 重的含意近於莊重穩重, 拙就是筆拙或質朴自然的意味, 大是與小相對的槪念. $lt;蕙風詞諸$gt;裏沒有對重拙大的直接說明, 可是按其評語可以探到重拙大意味的端緖. $lt;蕙風詞話$gt;裏況周顚提出重拙大就是作詞的三要. 重就是沈着, 可謂是深厚的作品內容充分地發現於作品之外表的. 拙是博統美學槪念上之巧不如拙的拙, 要求表現方面的自然和內容方面的眞率. 大是作品的氣象或意境的廣大, 這就誤寄託有密切關係. 況周願把重拙大析爲三個名辭, 而其意味領域難以分開. 從(蕙風詞話)的評語看, 況周願把重拙大看是一個統一槪念的詞語. 按筆者的分析,重拙大的意味領城包含如何的幾個特徵. 一, 作品裏充滿著作家深厚的情感而內容豊雷, 作品的可容性浿大. 二, 筆法裡自然, 作家的眞函貌具現得眞率, 因而充滿著生機. 三, 作品之外表洋濫著深遠的韻致,餘談無窯窮.

謝章鋌 詞論 硏究 : 詞의 문학성 회복과 관련하여

李奭炯 韓國中國語文學會 2000 中國文學 Vol.34 No.-

謝章鋌是淸末민詞壇的領油人物, 被評爲堅持比軟公正而客觀的論詞態度. 其<賭棋山莊飼話>所錄的論詞觀點是博看歷代詞籍而取捨選揮的結果. 他對詞的認識大體上以浙西詞派爲中心而折衷常州詞派的看法. 他親自體會淸末混亂的社會和失去方向的詞壇, 謨求詞學的未來發展方向. 本稿就是以謝章錠對未來詞學應該如何發展的深求爲重要問題來商討的. 詞本來是音樂的歌詞. 在淸末雖然詞樂佚傳已經?久了, 可是詞人仍然注意詞的音樂成分. 詞樂佚傳的情況, 詞的合律典否賞際上不可證賞, 因而對詞音樂性的論議흔可能流於空論. 謝章綎親眼看到浙西詞派末流和常州詞派末流所呈現的文學性破壞現狀的嚴重性, 看淸了重視音樂性的詞學態度是沒意義的, 主張要恢復詞的文學性. 其內容槪括了對性情的强調和題材範園的績大. 這些主張對於提高從來被認爲小道的詞文學地位和社會償値設服力復張. 可是這使詞失去其整體性, 反而加速詞的詩化.

폐경기 여성에서 호르몬 대치 요법시 병행 투여된 프로제스토젠 투여 방법에 따른 골대사의 생화학적 지표에 미치는 영향

이권해,강선영,임경빈,이해혁,구도형,이석민 순천향의학연구소 1998 Journal of Soonchunhyang Medical Science Vol.4 No.1

Purpose : To evaluate the impact of added progestogen on biochemical markers of the bone turnover undergoing hormone replacement therapy in the postmenopausal women. Materials and Methods : There are 71 postmentopausal women who are divided into three groups (Group I, Group II, Group III). Group I(continuous combined)is given conjugated equine estrogen (CEE, 0.625mg, day 1-30) plus medroxyprogesterone acetate (MPA, 2.5mg, day 1-30), Group II(unopposed estrogen) is given conjugated equine estrogen (CEE, 0.625mg, day 1-30) plus medroxyprogesterone acetate (MPA, 5mg, day 1-12). And there were measured serum osteocalcin, serum total alkaline phosphatase and urinary deoxypyridinoline excretion before starting hormone replacement therapy and 12 months later. Results : The serum osteocalcin, serum total alkaline phosphatase and urinary deoxypyridinoline excretion significantly decreased after hormone replacement therapy (Group, I, II, III). And there was no significance in decreases in biochemical markers of the bone turnover among three groups. Conclusions : It was concluded that the impact of added progestogen on the biochemical markers of the bone turnover undergoing hormone replaced therapy in postmenopausal women was not significant than nothing added group. Thus we concluded that progestogen was not impact on decreasing on the biochemical markers of the bone turnover in postmenopausal women undergoing hormone replacement therapy.

朱彛尊 詞論 硏究

이석형 韓國中國語文學會 2003 中國文學 Vol.39 No.-

朱이尊的詞學理論是爲了糾正淸初詞學中興時期詞壇出現的弊端而提出的. 他廣博閱讀詞籍,幷將主意力集中干南宋詞的變化上, 對慢詞體表現技法的巧妙給子了高度的評价,糾正了只重視五代北宋詞的云間詞派的狹益觀点, 將姜?推崇爲成就最高的作家, 從而明確了與主張學習辛棄疾的陳維崧 · 學習李煜的納蘭性德等的詞學路線的區別, 提出了浙西詞派的理論方向. 同時糾正了淸初詞壇低俗的氣韻及不和諧的音律, 爲了在詞中能구體現出雅正的情緖, 將醇雅列爲論詞的最高標准. 但是他的醇雅論只集中干詞的言語 · 音律的和諧 · 表現技法等形式方面的問題, 而忽略了對內容方面的論述, 因此他的理論幷不徹底. 其原因可歸干淸朝文字獄等森嚴的思想統治社會背景下有意識地回避現實問題的明哲保身的漢族出身知識分子自我保護的本能. 朱이尊在詞的創作上爲他人作出了傍樣, 提出了符合時宣的詞學理論, 一掃當時沈干鄙俗的詞風, 倡導了浙西詞派, 可謂爲其功; 但浙西詞派的詞風內容空洞逐漸流干空疏, 陷干두정, 這些與其理論的不徹底性又是分不開的.