Novel Methylation Biomarker for Non-invasive Diagnostics in Lung Cancer

오태정,( Chang Hun Lee2 ),( Min Ki Lee ),( Yeul Hong Kim ),( Sang Yull Lee ),( Hyo Sung Jeon ),( Shin Yup Lee ),( Seung Soo Yoo ),( Jae Yong Park ),( Sung Whan An ) 대한결핵 및 호흡기학회 2012 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.114 No.-

To identify aberrantly hypermethylated DNA in lung cancer cells we established a genome-wide analysis for hypermethylation sites, namely Methyl DNA Isolation and Amplification (MeDIA) coupled-CpG microarray analysis. In the comprehensive methyaltion profiling analysis between human lung cancer, A549 cells and normal NHBE cells, we observed that several clusters of genes show a significant level of aberrancy in CpG island methylation pattern in cancer cells compared to normal cells. We further identified PCDHGA12 gene as a new marker of non-invasive diagnostics for lung cancer based on followings. 1) Transcription of PCDHGA12 gene is reactivated after treatment of A549 cells with demethylating agent. 2) Bisulfide clonal-sequencing reveals that CpG island of PCDHGA12 shows a distinctive differential methylation between two cell lines. 3) Pyrosequencing-based quantitative methylation assay for such region in tumor and non-tumorous tissues from lung cancer patients shows aberrant hypermethylation in 37 (92%) of the 40 tumor tissues. In clinical validation by pyrosequencingin induced-sputum of lung cancer patients (n=87) and healthy controls (n=51), we observed aberrant hypermethylation incident at significantly elevated level in samples derived from lung cancer patients. According to the optimal threshold calculated by ROC curve analysis, sensitivity and specificity of PCDHGA12 was 86.2% and 82.4%, respectively. PCDHGA12 methylation status could be a potential methylation biomarker alone or combined with others for the screen and the detection of relapse of lung cancer.

Agrobacterium을 이용한 내열성 유전자의 벼로의 형질전환 및 발현

이병현,이효신,원성혜,조진기 경북대학교 농업과학기술연구소 1999 慶北大農學誌 Vol.17 No.-

To investigate the function of chloroplast-localized small HSP in rice, the cDNA, Oshsp21, was introduced into rice plants via Agrobacterium-mediated gene transfer system. Calli induced from rice immature embryos were co-cultivated with a A. tumafaciens EHA101 that contained a plasmid, pIHSP21. The efficiency of plant regeneration from the calli co-cultivated with the Agrobacterium was about 30%. PCR and Southern blot analyses using genomic DNA revealed that gene for the chloroplast small HSP was introduced into the genome of rice. Expression of transgene was investigated by northern blot analysis. Results indicate that the transgene, Oshsp21, was constitutively expressed at normal growth temperature.

요리활동을 통한 발달지체 유아의 주의집중 및 변별력 변화

이효신,신은희 대구대학교 특수교육재활과학연구소 2005 특수교육재활과학연구 Vol.44 No.3

본 연구는 발달지체 유아들에게서 공통적으로 낮게 발견되는 특성 중의 하나인 주의집중 및 변별력의 향상을 위하여 시도되었다. 이를 위하여 무엇보다도 유아들이 흥미를 가지는 매체를 선정하는 것이 중요하였으며, 선행연구의 검토를 통하여 이것은 요리활동으로 선정되었다. 즉, 본 연구의 목적은 요리활동을 통하여 발달지체유아들의 주의집중 및 변별력의 긍정적 변화를 확인하는 것이다. 연구대상으로 PEP에 의하여 발달지체를 나타내는 유아 4명을 선정하였고, 독립 및 종속변인(주의집중)의 기능적 관계를 확인하기 위하여 중다기초선설계를 이용하였으며, 또 다른 종속변인(변별력)의 변화는 프로그램 적용의 전후검사를 이용하여 확인하였다. 연구결과, 4명의 연구대상은 프로그램의 적용에 따라 급격한 주의집중 행동변화를 보임으로써 요리활동과 주의집중 행동의 기능적 관계를 확인할 수 있었다. 변별력은 4명 모두 상승된 점수를 나타내었는데 그중 유아 B가 가장 큰 상승을 나타내었다. 본 연구를 통하여 요리활동이 발달지체 유아들의 주의집중 행동을 긍정적으로 변화시킬 수 있음이 확인되었다. 그러나 향상된 주의집중 행동의 일반화 여부는 확인되지 않았으므로 이에 대한 후속 중재 및 연구가 필요하리라고 본다. 또한 변별력은 전후검사를 통하여 확인된 것이므로 미세한 행동의 변화를 측정할 수 없었음이 고려되어 해석되어야 할 것이다. The purpose of this study was to explore the effects of a cooking program on the sustained attention and the visual discrimination of children with developmental delays. The subjects for this study were 4 children with developmental delays enrolled in special kindergarten G, who scored below 50 developmental quotient on the Psycho-educational Test. Multiple Baseline Design Across Subjects (MBDAS) was used to determine the effects of a cooking program on the sustained attention and discrimination of children with developmental delays. In this study, MBDAS was performed on sustained attention while the children were playing house. The 8 types of cooking intervention program was conducted a total of 15 sessions. It was included while the children were playing house for duration of 30 to 40 minutes everyday. The data collection for sustained attention was completed every 10 seconds intervals for 10 minutes. The results of this experiment indicate that the cooking intervention program was very effective in increasing sustained attention. Before and after the intervention program, a visual discrimination test was performed to explore the effects of the cooking activity on discrimination, for children with developmental delays. The contents of the discrimination test sheet were extracted from an existing test sheet and reorganized by the researcher. The results of this test demonstrate that the cooking program positively influenced children with developmental delays in regards to discrimination and development of concepts. In summary, the cooking program proved to be effective in increasing sustained attention in children with developmental delays. The cooking program also positively influenced discrimination, specifically color, shape, and classification discrimination. The results of this study suggest a need for developing an interesting, specific, well-structured curriculum for children with developmental delays, which emphasized a variety of learning programs integrated within a cooking course.

아라키돈산 및 아이코사펜타엔산 생성 곰팡이의 분리 및 생산

신효선,오인호,이장한 동국대학교 산업기술환경대학원 1994 산업기술논총 Vol.2 No.-

Fungal strains producing extraordinary high amounts of arachidonic acid(ARA) and eicosapentaenoic acid(EPA) were isolated from soil samples and the effects of various culture condition on the production of ARA and EPA were investigated. Three strains of fungi with the high acids yields were identified as Mortierella alpina 49, M. parvispora 58 and M. horticola 73. Highest amounts of EPA and ARA were produced when three strains were cultivated at 18℃ for 4 and 6 days, respectively. When the three strains of fungi were grown in a medium with increase of 5% glucose, EPA production was decreased in every case, whereas ARA production not decreased in every case. There was no significant effect of oxygen on the fungi growth. But the production of EPA and ARA as well as the total fatty acids decreased when cultivated in 200 ml of culture volume in 500 ml flask. When the fungi cultivated for 6 days at 18℃ and stand for 6 days at room temperature, there was no difference in growth amount, while there was increase in the total fatty acid production, i.e., 36% increase as for M. parvispora 58 and 10∼20% as for the other two fungi. Aging didn't influence the EPA production much. the amount of the fungi growth and the total fatty acid production increased with more soybean oil addition as a nutrient of culture medium.

Sol-Gel Process를 利用한 Y-SrTiO_3 微分體 合成

申孝順,이병교 경북대학교 산업기술연구소 1992 産業技術硏究誌 Vol.20 No.-

Y-SrTiO_3 powders are synthesized by pechini process as a kind of sol-gel process and compared with powders by solid state reaction with respect to powder properties. As a result, calcination temperature is lowered about at 500℃. Furthermore, obtained powders are fine and homogeneous. And these powders are agglomerated and the size of primary particles is about 30㎚.

노부모의 성인자녀와의 갈등 및 우울에 관한 연구

이윤정,이신숙,신효식 全南大學校家政科學硏究所 1994 生活科學硏究 Vol.4 No.-

This study was to investigate the depression of the aged parents perceived through the conflicts between the aged parents and their adult children. The subjects of the study were 469 old parents, consisted of 234 male and 235 female over 60 years old lives in Kwangju. The datas were analysed by frequency, percentage, mean, standard deviation, oneway ANOVA, t test, Duncan's test and multiple regression using SAS program and the results are as follows. 1) In the conflict between the aged parents and their adult children which the aged parents perceive, the mean values are 32.54 in the case of old father and 34.26 in old mother from the average point. The average values of the depression the aged parents perceive are 26.03 and 28.35 each in old father and old mother and show the level of depression over the middle value. 2) The background variables which show the significant differences of the conflict between the aged parents and their adult children are marital status, age, education, pocket money, health and activity of the aged parents. 3) The background variables which show the significant difference in the depression the aged parents perceive are marital atatus, education, pocket money, health, activity, support type and occupation of the aged parents. 4) The depression and conflict between the aged parents and their adult children show high correlations and the greatest variable as the effect of conflict on the depression is emotional conflict. In addition, significant difference is shown depending on health, value and economic conflict and its explanatory power is 50%.

MnO₂를 첨가한 (Ni⅓Nb⅔)O₃-PbTiO₃-PbZrO₃계의 압전기적 특성

신혁수,이기태,남효덕 嶺南大學校 工業技術硏究所 1989 연구보고 Vol.17 No.2

The dielectric and piezoelectric properties of the ternary system of 0.2Pb₃?? and the effects of MnO₂ addition on the dielectric and piezoelectric properties were investigated. Specimens were calcined at 850℃ for 2hrs and sintered at 1200-1260℃ for 2hrs. The experimental results indicated that the specimens containing 42.5mole% PbTiO₃and 0.9wt.% MnO₂ showed the best dielectric and piezoelecric characteristics. The Curie temperature was decreased with increasing of mole% PbTiO₃. Though the dielectric constant was decreased with increasing of mole% PbTiO₃, Kp and Qm were increased. The addition of MnO₂ affected Qm more than Kp.

심상성 루프스에서 Nested-Primer Gene Amplification Assay에 의한 결핵균 DNA의 검출

신문석,전효진,김병천,이규석,김재룡,권호준,송준영 啓明大學校 醫科大學 1994 계명의대학술지 Vol.13 No.4

Lupus vulgaris, which is a progressive form of postprimary tuberculosis in patients with a moderate to high degree of immunity and tuberculin sensitivity, is the most common, most serious, and most variable type of cutaneous and mucous membrane tuberculosis. Domonstration of M.tuberculosis directly or in culture in some of these eruptions can be difficult. It is essential to demonstrate the presence of M.tuberculosis in a clinical sample for a definitive diagnosis of tuberculosis. With the discovery of the polymerase chain reaction (PCR), the DNA diagnostic approach for infection has developed in a short period. With the dual purpose of definitive and rapid diagnosis of tuberculosis, this study examined the usefulness of a nested PCR for detection of M. turberculosis. Compared with a single-step PCR, the two-step PCR was able to enhance sensitivity approximately 1,000-fold on the basis of bacterial counts and DNA quantity. We concluded that two-step nested PCR appeared to be the most useful PCR protocol for early and sensitive diagnostic method detecting M.tuberculosis in acid-fast stain negative specimens.

矢狀分割骨切斷術에 依한 顎矯正術의 統計學的 硏究

辛曉根,陳宇政,李俊禮,金晤煥,李炫尙 大韓顎顔面成形再建外科學會 1997 Maxillofacial Plastic Reconstructive Surgery Vol.19 No.2

Recently, combined with social complexity, increment of medical demand and supply and the change of esthetic category, admission and operation of the patients of facial deformity have been changed with annual change. This study was conducted in the concept of helping the overall character of orthognathic surgery in future understood and being an important index in the establishment of better treatment course, through the patient, on whom was operated orthognathic surgery via sagittal split ramal osteotomy in our Dept. of Oral & Maxillofacial Surgery, Chonbuk National University Hospital from 1991. 1. 1 to 1995. 12. 31. The results were obtained as follows. 1. The ratio of male to female was 1 : 1.33 and the ages between 16 and 25 year was 73.6%, and the mean age was 20.4 years. 2. The ratio of setback amount between 6 to 15mm was 84.6% and the advancement amount between 1 to 10mm was 89% and the mean amount of movement was 9.0mm in setback, and 3.6mm in advancement. 3. After removal of maxillomandibular fixation(MMF), the distinction of sex was not statistically significant in ROM. 4. The ROM following methods of fixation was statistically significant in 3rd(P<0.05), 8th, and 12th week(each P<0.01), with faster rehabilitation in rigid fixation which had shorter MMF period. 5. The rehabilitation of ROM following the operation methods was statistically significant in 1st(P<0.05), 2nd, 3rd, 4th, 6th, 8th, and 12th week(each P<0.01) with faster rehabilitation in the case of SSRO than Lefort I / SSRO (Two jaw surgery). 6. The rehabilitation of ROM following directions of mandibular movement did not manifest a statistically significant difference in both setback and advancement.

벼 Small HSP의 발현에 의한 대장균의 고온 stress 하에서의 내성의 증가

이병현,이효신,원성혜,조진기 경북대학교 농업과학기술연구소 1999 慶北大農學誌 Vol.17 No.-

This study was conducted to detect the allelopathic effect of water and ethanol extracts from 4 plant species (Lactuca sativa L., Ambrosia elatior L., Oenothenra odorata Jasp., Ginkgo biloba L.) on germination and growth of lettuce seeds. A little bit higher inhibitory effect was obtained in ethanol extracts rather than water extracts. Allelopathic effects varied in the source of extracts and concentrations of 4 plant species and the highest allelopathic effect appeared in 10% solution, regardless of plant species.