Identification of pepper genes involved in the response to CO 2 enrichment using RNA-Seq analysis

Jing Zhang,Rui Bai,Mengya Shang,Xiaoyong Xu,Hongxia Song,Shaowen Zheng,Leiping Hou,Meilan Li,Guoming Xing 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.1

Pepper is widely cultivated, and the application of CO 2 promotes photosynthesis and increases its yield. However, the molecularmechanisms underlying this are still unclear. In this study, the photosynthetic correlation indexes under elevated CO 2and control conditions were compared. The application of CO 2 increased the photosynthetic capacity of pepper. Moreover,RNA-Seq analysis was used to identify genes that were diff erentially expressed between pepper leaves grown in CO 2 -enrichedconditions and those grown in control conditions. The 149 diff erentially expressed genes (DEGs) were found to be involvedin photosynthesis and other metabolic processes. According to GO signifi cant enrichment analysis, the proteins encodedby the DEGs were mainly found to be located in the chloroplast, the chloroplast matrix, and the apoplast. According toKEGG signifi cant enrichment analysis, the DEGs were found to be involved in glutathione metabolism; starch and sucrosemetabolism; and stilbenoid, diarylheptanoid, gingerol, fl avonoid, and phenylpropanoid biosynthesis. The DEGs were alsoinvolved in the pentose phosphate pathway, carbon metabolism, and porphyrin and chlorophyll metabolism. Based on theGO annotation and the KEGG database analysis, ten of the DEGs identifi ed were suggested to be involved in photosynthesisand related processes; these genes were predicted to have roles in carbohydrate, soluble sugar, and glutathione metabolism,and in raffi nose, cysteine, nucleotide, and ABA biosynthesis. These DEGs are involved in the pentose phosphate pathwayand tricarboxylic acid cycle of carbon assimilation during photosynthesis. One of the DEGs was also found to be involved inchlorophyll biosynthesis. These results lay the foundation for further investigation of the molecular mechanisms and genesinvolved in the response to CO 2 enrichment in peppers.

VOx-MoOy single molecular layer modified graphic carbon nitride polymer for enhanced selective styrene oxidation

Xinrui Jing,Hongxia Wang,Songnan Li,Bin Lu,Jingxiang Zhao,Qinghai Cai 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.105 No.-

Graphic carbon nitride polymer was modified with transition bimetals compounds by impregnation ofpre-prepared g-C3N4 in aqueous solution of ammonium molybdate and vanadate. The prepared hybridmaterial was characterized by XRD, SEM, TEM, FT-IR, XPS and N2 adsorption–desorption techniques todetermine its surface morphology, composition and structure. The results revealed that VOx-MoOy singlemolecular layer incorporated into g-C3N4 molecule framework by interaction between host and guestwas fabricated. The hybrid composite was employed as catalyst for styrene oxidation to benzaldehydeusing molecular oxygen as oxidant. The results indicated that the composite was highly active and selectivefor styrene oxidation, giving TON 458.8 mol/mol(Mo+V) and 96.7% selectivity to benzaldehyde. Thecorrelation between high activity and surface properties the catalyst was explored.

A Novel Control Method for Drum Level and Pressure Basing on Dynamic Surface

Yuanwei Jing,Hongxia Yu,Xiaoyu Sun 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.6

A novel drum level and pressure simultaneously control method is developed using the dynamic surface control method in this paper which takes account of the output power of units and load change .Certain first-order low-pass filters are introduced into the designing process to avoid the occurrence of high-order derivatives of elements in the system which makes it easy to implement in practical applications. The proposed control method is effective in compensating for the disturbance of load and fuel. Simulation results show that the dynamic surface control method still ensures an accurate result, even if the loads change in a great and parameters of the controlled plant change significantly.

Evaluation of sex attractants for monitoring of Illiberis ulmivora Graeser (Lepidoptera: Zygaenidae: Procridinae): Height, distance, placement, climatic conditions

Liu Hongxia,Dou Rui,Wang Jifei,Zhu Yachao,Li Jingyao,Liu Jinlong,Wang Yang,Zhang Jintong,Jing Xiaoyuan 한국곤충학회 2022 Entomological Research Vol.52 No.2

The phytophagous caterpillar Illiberis ulmivora is a serious pest of Ulmus glaucescens Franch (Ulmaceae, Ulmus). In order to test potent sex attractant, field trails of synthetic candidate attractants were conducted in China. In this study, baits with ternary blends, (2S)-butyl (7Z)-dodecenoate, (2S)-butyl (9Z)-tetradecenoate, and (2S)-butyl (5Z)-decenoate (8:4:1 ratio) resulted in a higher number of male moths capture than the rest of the baits that were offered. Traps positioned at a height of 1.2 m attracted more males than those installed at heights of 2.2 m and 3.2 m. The slope aspect, temperature and humility had a significant effect on the trap catches. More catches occurred in traps placed on semi-shady and semi-sunny slopes than those placed on sunny and shady slopes. The number of pheromones in the septa decreased with age. The residual amounts of pheromones in the lures combined with 2,6-di-tert - butyl-p-cresol (BHT) were higher than those in the control. However, there were no differences in the male captures between components and components combined with BHT during the first 8 d. These findings on monitoring and trap positions can be used to predict the occurrence and population dynamics of I. ulmivora, to develop ecological management of I. ulmivora.

Effect of some plant essential oils on the orientation and predation capacity of the predatory spider Pardosa pesudoannulata

Ibrahim Maholidy Farid,Hamada Chakira,Wanlun Cai,Jing Zhao,Hongxia Hua 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.3

Investigating the potential side-effects of plant essential oils to natural enemies is necessary before applying them in the field to control insect pests. In the present laboratory study, the attractancy/repellency of two plant oils to the spider Pardosa pseudoannulata was evaluated using a paired choice method. An individual spider was allowed to choose between the odors of essential oils extracted from Piper nigrum, Litsea cubeba, or their mixture and the control within 10 min. The predation deterrent was evaluated by observing the predation of caged spiders supplied with 20 nymphs or adults of Nephotettix cincticeps pre-treated with essential oils in 24 h. Results revealed that P. nigrum and L. cubeba oil had no significant influence on the orientation of the predator in all tested concentrations. However, the mixture of P. nigrum and L. cubeba triggered significant attraction to P. pseudoannulata at 100 and 1000 ppm. Significant reduction of predation was observed with increasing concentration of the mixture and P. nigrum alone. The predation rate in the L. cubeba assay was not significantly different among the tested concentrations. In all cases, predation was higher on nymphs than on adult stages. We did not observe any interaction effect between the life stages and concentrations. To our knowledge, this is the first study discussing the potential influence of plant essential oils on orientation and predation of P. pseudoannulata. Based on these results, some essential oils and their combinations may influence the orientation of P. pseudoannulata and might affect its predation capacity. Further field studies are needed, but these findings suggest caution regarding the selection and application of plant extracts used for biological control in rice fields.

De novo transcriptomic analysis to reveal insecticide action and detoxification-related genes of the predatory bug, Cyrtorhinus lividipennis

Siyi Liu,David R. Nelson,Jing Zhao,Hongxia Hua,Yueping He 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.2

The mirid bug, Cyrtorhinus lividipennis Reuter, an important predatory natural enemy of rice planthoppers, is widely distributed in rice fields. However, genetic information on C. lividipennis is lacking. Especially, limited data about mechanisms of insecticide selectivity between this piercing-sucking predator (C. lividipennis) and piercing-sucking preys (rice planthoppers), inhibits development of selective insecticides and the integration of chemical and biological control systems to control insect pests of rice. Hence, we performed de novo assembly of a transcriptome from adult and nymph whole bodies of C. lividipennis. A total of> 29 million of reads were generated, and 34,752 transcripts matched known proteins. Then, the genes related to insecticide action and detoxification were manually identified, including 26 carboxylesterases (containing 2 acetylcholinesterases), 57 cytochrome P450s, 19 glutathione S-transferases, 15 nicotinic acetylcholine receptors, 3 GABA-gated ion channels, and 1 glutamate receptor. Comparisons of sequence differences in these genes between C. lividipennis and rice planthoppers, revealed that quite a lot of diversity was found among genes related to insecticide action and detoxification, while a few of these genes share much higher identities between this predator and prey. The present study provides useful information for our understanding of insecticide selectivity between rice planthoppers and the predator mirid bug.

Gene-Edited Human Kidney Organoids Reveal Mechanisms of Disease in Podocyte Development : Podocyte Development and Disease in Organoids

Kim, Yong Kyun,Refaeli, Ido,Brooks, Craig R.,Jing, Peifeng,Gulieva, Ramila E.,Hughes, Michael R.,Cruz, Nelly M.,Liu, Yannan,Churchill, Angela J.,Wang, Yuliang,Fu, Hongxia,Pippin, Jeffrey W.,Lin, Lih Y AlphaMed Press, Inc. 2017 Stem Cells Vol. No.

Behavioral response of Nilaparvata lugens (Stål), Cyrtorhinus lividipennis Reuter and Paederus fuscipes Curtis to three synthetic volatile chemical compounds

Khan Muhammad Musa,Huang Qing,Wagan Tufail Ahmed,Hua Hongxia,Cai Wanlun,Zhao Jing 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.2

Plant essential oils (EOs) and a wide range of chemicals affect insect pests in many ways, such as via stimulatory, deterrent, toxic and hormonal effects. Three different compounds ((E)-β-caryophyllene (E-β-C), D-limonene (Dlime) and trans-2-dodecenol (T-2-D)) were tested against Nilaparvata lugens, Cyrtorhinus lividipennis and Paederus fuscipes, and their behavioral response was assessed. The results showed that on average, more N. lugens nymphs were repelled by E-β-C and T-2-D than by D-lime. More C. lividipennis nymphs were attracted to T-2-D and D-lime than to E-β-C. However, P. fuscipes displayed no significant response to the three chemical compounds. The results also demonstrated that T-2-D has exerted significant repellency against N. lugens and a significant attraction for C. lividipennis, while E-β-C and D-lime have no significant effect on any tested insect. T-2-D was selected and tested in a greenhouse under semi-field conditions, where the observations confirmed the results of the laboratory experiments. From the results, it can be concluded that T-2-D at a concentration of 0.06 g/L is an effective synthetic volatile chemical compound and is the strongest repellent of N. lugens and the strongest attractant for C. lividipennis. This synthetic chemical compound can be used as a pest management tool in rice agroecosystems.

Preparation of epoxypropyl functionalized graphene oxide and its anticorrosion properties complexed with epoxy resin

Xiaofeng Yang,Yuepeng Zhang,Zhiping Chen,Yinshuai Yang,Hongxia Jing,Zhenhao Sun,Huijie Wang 한국화학공학회 2020 Korean Journal of Chemical Engineering Vol.37 No.12

Functionalized graphene oxide (GO)/epoxy resin (EP) composite coating has attracted attention due to its advantages of epoxy resin in acid resistance, anticorrosion and having the characteristics of GO such as good chemical stability and strong electrolyte barrier properties. In this study, epoxypropyl functionalized graphene oxide (EFGO) was synthesized with a new strategy by grafting epichlorohydrin (ECH) onto the surface of GO. EFGO was then crosslinked with EP by diethylenetriamine (DETA) to fabricate EFGO/EP anticorrosive coating. The structure and property of EFGO and EFGO/EP were characterized and evaluated by various methods such as FTIR, Raman, XPS, SEM, and EIS. The results showed that the graft amount of epoxypropyl on GO surface could be effectively increased by using KI-KF catalysis system. The increase of the graft amount further improved the compatibility between EFGO and EP. EFGO/EP had a network structure with EFGO as the “central node”. The “central node” locked the movement of EP chain segment, as improving the thermal stability of EFGO/EP. The EFGO/EP coating fabricated by the EFGO, which grafted larger epoxypropyl, had good anticorrosion performance and adhesion with steel plates, showing a good barrier effect on electrolyte migration. The coating resistance reached up 4.88×108 cm2.

KCl flux-assisted CaTiO3: Pr3+ red phosphors for temperature sensing

Xin Liu,Xiuying Tian,Huiyan Sheng,Changyan Ji,Zhi Huang,Jin Wen,Hongxia Peng,Ling Zhu,Jing Li,Ping Liu,Yangxi Peng,Guowen Li 한양대학교 청정에너지연구소 2024 Journal of Ceramic Processing Research Vol.25 No.2

The red CaTiO3: Pr3+ phosphors were fabricated by employing a solid-state method using KCl flux, and investigated fortemperature sensing. There were no structural changes observed with increasing KCl concentration, and no impuritieswere detected. The obtained crystal structure was identified as an orthorhombic perovskite, belonging to space group ofPnma(62). The typical sample exhibited spherical particles (1-2 μm). The optical bandgap of the sample was measured to beapproximately 3.62 eV. Notably, when the KCl concentration reached 3%, the sample exhibited the highest photoluminescenceintensity, indicating excellent crystallization strength. They closely resembled the chromaticity coordinates of ideal red lightbased on CIE chromaticity diagram, and the color purity was determined to be 79.51%. Furthermore, the absolute sensitivity(Sa) was approximately as high as 0.148 K-1, while the relative sensitivity (Sr) reached approximately a high value of 6.57% K-1,which highlights the significant potential of optical thermometry.