Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

구리 지역에서 조사한 고혈압의 인지도, 치료률 및 관리률

홍철호,김순길,홍택원,이제,신진호,이재웅,김경수,김정현,임헌길,이방헌 대한간질학회 2003 대한간질학회지 Vol.9 No.1

Multiple Markers of Contrast Induced Nephropathy after the Percutaneous Coronary Intervention

Byoung-won Park,Seong Soon Kwon,Min Ho Lee,Do Hoi Kim,Min Su Hyon,Duk Won Bang 순천향대학교 순천향의학연구소 2018 Journal of Soonchunhyang Medical Science Vol.24 No.1

Objective: Contrast-induced nephropathy (CIN) frequently occurs after percutaneous intervention. Objective of this study was to investigate the usefulness of serum cystatin C, neutrophil gelatinase-associated lipocalcin (NGAL), urinary kidney injury molecule-1 (KIM-1), and interleukin-18 (IL-18) as early predictors for CIN after percutaneous coronary intervention (PCI). Methods: In 53 patients who underwent PCI were enrolled. Serum creatinine and cystatin C level were measured immediately before, and 24 hours and 48 hours after catheterization. Serum NGAL, urinary KIM-1, and IL-18 were measured immediately before, and 4 hours, 24 hours, and 48 hours after catheterization. CIN was defined as a rise in creatinine 0.5 mg/dL or 25% above baseline. Results: CIN occurred in four patients (7.5%). Serum cystatin C levels were higher at 24 hours and 48 hours in CIN patients than in those without CIN (P<0.05). Serum NGAL levels were higher at 48 hours in CIN patients than in those without CIN. Urinary KIM-1 levels were higher at 48 hours in CIN patients than in those without CIN. There were no significant markers of CIN on multi-variate analysis. Conclusion: In this study, the occurrence of CIN after PCI was 7.5%. Although there were some time-course changes in serum cystatin C and urinary KIM-1 after PCI, there was no significant predictor for CIN after PCI.

혈부축어탕의 고지혈증 유발 생쥐 신장내 지방 축적 감소효과

김호현,강윤호,방혜정,김진택,박인식,안상현,이해풍 世明대학교 한의학연구소 1998 韓醫學硏究所 論文集 Vol.1 No.-

Renal tissues of ICR mouse were intraperitoneally injected with Hyulboochucketang(HCE;1.28g/kg/day) after Triton WR-1339(TX;600mg/kg) injection were observed to investigate the lipid accumulation suppressive effect of HCE. The renal tissues were obtained at hour 24,48 and 72 after TX injection that were fixed in fromol-calcium solution and were cryocut. The tissue stained by H&E for general morphology, sudan black B for lipid and perchloric acid-naphthoquinone method for cholesterol. The parietal layer of Bowman's capsule is swelled as cuboidal type at hour 48 after TX injection and the lipid blot and cholesterol particle were noticeably increased in glomerulus than control group. But the parietal layer of Bowman's capsule in HCE treated group was shaped as squamous type that was looked like as normal morphology and the lipid blot and cholesterol particle w3ere considerably decreased in glomerulus than TX group. As results indicated that the accumulation of lipid including cholesterol caused by TX injection were mitigated in renal tissues by the antihyperlipidermic effect of HCE.

주행차량에 의한 강교량의 충격계수에 관한 연구

김상효,이상호,김방래 연세대학교 산업기술연구소 1996 논문집 Vol.28 No.1

In this study, a finite element program which can perform the dynamic analyses of bridges by considering the interaction forces between the bridge and moving vehicle is developed. The impact factors and dynamic behaviors of bridges according to the various parameters, such as speed and weight of vehicles, span length, damping ratio, girder spacing and transverse stiffnesses of bridges, are studied. The impact factor, in general, increases as the traveling speed increases, whereas the weight of vehicle does not affect the impact factor. The impact factor is larger in exterior girders than in interior girders. As the spacing between two girders increases, the impact factor decreases, and it is also affected by the lateral rigidities of the bridges. In addition, the impact factor is smaller in continuous bridges than in simply supported bridges.

E-business가 지식경영활동에 미치는 영향

방호열,김성호 국제무역학회 2002 국제무역연구 Vol.8 No.1

E-business가 지식경영에 미치는 영향을 파악하고 하는 것이 본 연구의 목적이다. 이를 위해서는 E-business를 세 가지의 차원(E-business 발전단계, E-business 전략, E-business 정보시스템)으로 분류를 하였고, 지식경영활동은 지식이전·지식활용의 차원으로 분류를 하여 분석을 실시하였다. 연구의 모형은 E-business가 지식이전에 영향을 미치고 지식 이전은 지식활용에 영향을 미친다라고 설정을 하였다. 제시된 가설을 분석한 결과는 다음과 같다. E-business의 발전단계와 정보시스템은 지식이전에 부분적으로 영향을 미치고 지식이전은 지식활용에 있어서 부분적으로 영향을 미치는 것으로 나타났다. The purpose of this study is that E-business affects knowledge management. To achieve E-business suggests three dimensions(E-business development stages, E-business strategy, E-business information system) and knowledge management activities do knowledge transfer application. The research model of this paper is that E-business affects knowledge transfer, knowledge transfer affects knowledge application. To test model survey research is conducted. Data gathered from Korea Manufacturing firms that adopted E-business. The empirical results show that E-business development stages and information system partially affect knowledge transfer, knowledge transfer also partially affects knowledge application.

Retinoic Acid가 흰쥐 태자 혀조직의 분화와 Laminin 합성에 미치는 영향에 대한 연구

김혜주,정호삼,서윤경,백두진,김원규,윤지희,이방헌 대한췌담도연구회 2000 대한췌담도연구회지 Vol.5 No.4

E-business 발전단계와 전략

김성호,방호열 국제무역학회 2003 국제무역연구 Vol.9 No.1

최근에 인터넷은 상업적인 수단으로써 그 중요성이 증가함으로써 기업들은 E-business를 빠르게 채택하게 되었다. 본 연구는 E-business를 채택하고 있는 기업이 그 발전단계가 상이하다고 보고 이를 탐색적인 차원에서 분석하고 한다. 이를 위해서 E-business의 발전단계와 E-business의 전략을 제시하였다. 이와 동시에 E-business의 발전단계와 전략과의 관계를 분석함으로써 현재 기업들이 채택하고 있는 E-business가 전략과는 어떠한 관계를 가지고 있는지를 파악하고자 한다. 이상의 목적을 달성하기 위하여 본 연구는 E-business를 채택하고 있는 기업들을 대상으로 설문조사를 실시하였고 수집된 자료를 군집분석과 ANOVA를 이용하여 분석을 실시하였다. 실증분석결과는 본 연구에서 제시하고 있는 E-business의 발전단계에 따라서 조사된 기업들 분류할 수가 있었다. 이렇게 분류된 기업들을 이용하여 E-business 전략과의 관계를 파악한 결과는 E-business 의 발전단계가 높은 기법일수록 더 높은 수준의 전략을 동시에 추구하고 있는 것으로 나타났다. In the last few years, the Internet has emerged as an important medium of commerce. Firms rapidly adopt E-business. As finns evolve, they pass through stage of E-business development. In this paper we propose E-business development stages and strategy. The relations between E-business stage and strategy is proposed. To test the relations survey research is conducted. Data gathered from Korea Manufacturing finns that adopted E-business. To analyze the data used Cluster analysis and ANOVA. The empirical results show that firms classify into groups as E-business development stages, it relates E-business strategy. It implies that the higher E-business development stages adopt the higher E-business strategy.

Bacillus subtilis의 cdd유전자의 Escherichia-Saccharomyces Shuttle Vector YRp7에의 클로닝

金榮基,宋邦鎬 경북대학교 과학교육연구소 1988 科學敎育硏究誌 Vol.12 No.-

The cdd gene enconding cytidine deaminase from Bacillus subtilis was cloned and expressed in the Saccharomyces cerevisiae D13-1A. By insertion of the 1.45 Kb of EcoRl/EcoRl fragment which containing genes fo for replication origin and trpl selective marker from Escherichia-Sac-charomyces shuttle vector YR_p7 into the EcoRl site of pS062, the Bacillus cdd gene was expressed in the yeast cells. By selection of the trp+ colonies in the SD medium. One of the transformed Saccharomyces cells were isolated as Saccharomyces cerevisiae YKl. The transformed yeast cells which carring chimeric plasmid, pYKl, was expressed the cdd activity with two folds elevated levels. For further confirming the pYKl expression in yeast cell, the pYKl which isolated from the transformed cells was recloned into the Escherichia coli KM268. The plasmid isolated from strain KM268 was identified as the same one with the original chimeric plasmid, pYKI.

자원준거시각에서 정보기술자원이 전자상거래의 전략적 편익과 성과에 미치는 영향

방호열,김성호 국제무역학회 2004 국제무역연구 Vol.10 No.2

본 연구의 목적은 정보기술자원이 전자상거래의 전략적 효과성과 기업의 성과에 미치는 영향을 파악하는 것이다. 이와 더불어 전자상거래를 수행하는 기업의 성과에 전자상거래의 전략적 효과성이 어떠한 영향을 미치는지를 파악하고자 한다. 본 연구에서는 정보기술자원을 유형자원, 무형자원, 기업능력으로 나누어 제시를 하였다. 그리고 전자상거래 수행 기업이 얻을 수 있는 전략적 효과성으로는 비용중심, 투자중심, 서비스중심, 수익중심의 차원을 제시하였다. 연구목적으로 달성하기 위하여 연구모형과 관련 가설을 제시하였다. 연구대상은 국내 제조기업 중에서 전자상거래를 수행하고 있는 기업이고 설문조사기법을 이용하여 자료를 수집하였다. 연구모형에 적합한 분석기법을 이용하여 연구모형을 분석한 결과는 다음과 같다. 전자상거래의 전략적 효과성 중에서 서비스 중심에 대하여 정보기술자원이 정의 영향을 미치는 것으로 나타났고 정보기술자원은 기업성과에 정의 영향을 미치는 것으로 나타났다. 전자상거래의 전략적 효과성 중에서 서비스 중심이 기업성과에 정에 영향을 주는 것으로 나타났다. The object of this study is to explore information technology resource affecting strategic benefits of electronic commerce and firms' performance. The other object is to explore benefits of electronic commerce affecting firms' performance. We propose that information technology resources composed tangible resources, intangible resources, firms capabilities. Strategic benefits of electronic commerce consist of service center, investment center, cost center, profit center. We proposed research model and related hypothesis. To test hypothesis of this study data are collected from manufacturing company in Korea by survey research. We analyse the research model by adequate statistical method. The result are as follows: (1) information technology resources affect service center of strategic benefits (2) information technology resources affect firms' performance. (3) service center of strategic benefits affects firms' performance.