Effects of age on intestinal phosphate transport and biochemical values of broiler chickens

Li, Jianhui,Yuan, Jianmin,Miao, Zhiqiang,Guo, Yuming Asian Australasian Association of Animal Productio 2017 Animal Bioscience Vol.30 No.2

Objective: The objective of this experiment was to characterize the mRNA expression profile of type IIb sodium-inorganic phosphate cotransporter (NaPi-IIb) and the biochemical values of serum alkaline phosphatase (AKP), calcium, inorganic phosphorus, tibial ash and minerals of broiler chickens with aging. Methods: A total of 56 one-day-old Arbor Acres male broiler chickens were used. Broiler chickens were weighed and samples were collected weekly from day 1. Results: The result showed that before the growth inflection point, ash, calcium, and phosphorus content in the tibia of broiler chickens increased with growth (before 3 weeks of age), although there were no significant differences in chicks at different ages in the later period of the experiment and weight gain rate was relatively slow at this stage (4 to 6 weeks). NaPi-IIb gene expression in the small intestine in the early growth stage was higher than that in the later growth stage. Expression of calbindin and the vitamin D receptor protein in the intestinal mucosa increased with age in the duodenum and jejunum. Serum AKP activity first increased and subsequently decreased after peaking at 1 week of age, but there was no significant difference after 3 weeks of age. Conclusion: These results show that compared with the early growth stage, the weight-gain rate of broiler chickens in the late growth stage gradually decreased with gradual tibia maturation, along with weaker positive transport of phosphorus in the intestine and reinforced re-absorption of phosphorus in the kidney, which might be the reason that phosphorus requirement in the late growth stage was decreased.

The Influence of Dietary Calcium and Phosphorus Imbalance on Intestinal NaPi-IIb and Calbindin mRNA Expression and Tibia Parameters of Broilers

Li, Jianhui,Yuan, Jianmin,Guo, Yuming,Sun, Qiujuan,Hu, Xiaofei Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.4

A $2{\times}2$ factorial experiment was conducted to study the effect of dietary calcium and non-phytate phosphorus (nPP) imbalance on calbindin and NaPi-IIb mRNA levels in the small intestine and tibia parameters of broiler chicks. One hundred and forty four 1-d-old Arbor Acres male broiler chicks were divided into four treatments consisted of six replicates with six chicks each. The two dietary calcium levels were 1.10% and 0.60%, and two dietary nPP levels were 0.50% and 0.27%. Results showed that a high Ca/nPP ratio diet (4.07:1) significantly depressed feed intake and weight gain of broilers (p<0.05), but a lower Ca:nPP ratio (1.2:1) had no influence (p>0.05). Low-Ca with low-P diet resulted in low tibia minerals and tibia breaking strength of broilers, and all the tibia parameters were further decreased when the dietary ratio of Ca to P was relative higher. Low dietary Ca or P up-regulated the calbindin and NaPi-IIb mRNA expression levels. Low Ca with normal P diet up-regulated duodenal calbindin mRNA expression level to the greatest extent. Low P with a normal Ca diet significantly enhanced NaPi-IIb mRNA expression level to the highest extent. These results suggest that the calbindin and NaPi-IIb mRNA expression were enhanced by the imbalance between dietary Ca and nPP, and their expression were not only influenced by Ca or nPP level, but also the ratio of Ca:nPP.

A new method to calculate the equivalent stiffness of the suspension system of a vehicle

Pinbin Zhao,Guo-feng Yao,Min Wang,Xumin Wang,Jianhui Li 국제구조공학회 2012 Structural Engineering and Mechanics, An Int'l Jou Vol.44 No.3

The stiffness of a suspension system is provided by the bushings and the stiffness of the wheel center controls the suspension's elasto-kinematic (e-k) specification. So the stiffness of the wheel center is very important, but the stiffness of the wheel center is very hard to measure. The paper give a new method that we can use the stiffness of the bushings to calculate the equivalent stiffness of the wheel center, which can quickly and widely be used in all kinds of suspension structure. This method can also be used to optimize and design the suspension system. In the example we use the method to calculate the equivalent stiffness of the wheel center which meets the symmetric and positive conditions of the stiffness matrix.

A new method to calculate the equivalent stiffness of the suspension system of a vehicle

Zhao, Pinbin,Yao, Guo-Feng,Wang, Min,Wang, Xumin,Li, Jianhui Techno-Press 2012 Structural Engineering and Mechanics, An Int'l Jou Vol.44 No.3

The stiffness of a suspension system is provided by the bushings and the stiffness of the wheel center controls the suspension's elasto-kinematic (e-k) specification. So the stiffness of the wheel center is very important, but the stiffness of the wheel center is very hard to measure. The paper give a new method that we can use the stiffness of the bushings to calculate the equivalent stiffness of the wheel center, which can quickly and widely be used in all kinds of suspension structure. This method can also be used to optimize and design the suspension system. In the example we use the method to calculate the equivalent stiffness of the wheel center which meets the symmetric and positive conditions of the stiffness matrix.

<i>Pseudomonas aeruginosa</i> injects NDK into host cells through a type III secretion system

Neeld, Dennis,Jin, Yongxin,Bichsel, Candace,Jia, Jinghua,Guo, Jianhui,Bai, Fang,Wu, Weihui,Ha, Un-Hwan,Terada, Naohiro,Jin, Shouguang Society for General Microbiology 2014 Microbiology Vol.160 No.7

<P><I>Pseudomonas aeruginosa</I> is a Gram-negative opportunistic human pathogen possessing a type III secretion system (T3SS) which injects toxic effector proteins into mammalian host cells. In previous studies, <I>P. aeruginosa</I> strains lacking all of the known type III effectors were shown to cause cytotoxicity upon prolonged infection time. In this study, we report the identification of a new cytotoxin, nucleoside diphosphate kinase (NDK), which is injected into eukaryotic cells in a T3SS-dependent manner. Injection of NDK is inhibited by the presence of previously known effectors of the T3SS, with an effectorless strain injecting the highest amount, suggesting active competition with the known T3SS effectors. NDK is shown to cause a cytotoxic response when expressed in eukaryotic cells, and <I>P. aeruginosa</I> strains harbouring NDK also show a greater toxicity than strains lacking it. Interestingly, the cytotoxic effect of intracellular NDK is independent of its kinase activity. In previous studies, NDK was shown to be secreted into culture supernatants via a type I secretion system and cause cytotoxicity in a kinase-dependent manner. Therefore, the current study highlights an alternative route of NDK secretion as well as two different cytotoxic mechanisms of NDK, depending on the extra- or intra-cellular location of the protein.</P>

Construction of a resequencing-based high-density genetic map for grape using an interspecific population (Vitis amurensis × Vitis vinifera)

Shi Guangli,Sun Dan,Wang Zhenxing,Liu Xiaoying,Guo Jianhui,Zhang Susu,Zhao Ying,Ai Jun 한국원예학회 2022 Horticulture, Environment, and Biotechnology Vol.63 No.4

A high-density genetic map is a prerequisite for marker-assisted selection of crop species. Here, we developed a high-density genetic map of Vitis using 92 F1 progeny derived from the cross between two wine grape cultivars, ‘Shuanghong’ and ‘Italian Riesling’. Whole genome resequencing (WGS) technology was used to generate SNP markers for the map. The map consisted of 1351 recombination bin markers (210,873 SNPs), distributed on 19 linkage groups (LGs), with an average distance of 0.65 cM between adjacent bin markers and a map distance of 860.46 cM. The high levels of uniform coverage and collinearity between the 19 LGs and the grape reference genome indicated that the map is of high quality and can serve as a powerful platform for evaluating grapevines and deciphering the genetic mechanism of important economic and agronomic traits. Our findings will facilitate the process of mapping quantitative trait loci (QTL) that control important horticultural and economic traits of grape.

Long Noncoding RNA Expression Profiling During the Neuronal Differentiation of Glial Precursor Cells from Rat Dorsal Root Ganglia

Yunfei Dai,Wei Ma,Tong Zhang,Jinwei Yang,Chenghao Zang,Kuangpin Liu,Xianbin Wang,Jiawei Wang,Zhen Wu,Xingkui Zhang,Chunyan Li,Junjun Li,Xiangpeng Wang,Jianhui Guo,Liyan Li 한국생물공학회 2020 Biotechnology and Bioprocess Engineering Vol.25 No.3

Long noncoding RNAs (lncRNAs) play important roles in the process of cell fate determination. However, their function and expression profiles have not yet been systematically investigated during the transdifferentiation of glial precursor cells derived from dorsal root ganglia (DRG) in the peripheral nervous system. Our results demonstrated significant differences in gene architecture and expression among the three transcript types (lncRNA, mRNA, and TUCP). Distinct differences in transcript length, exon number, and ORF length were identified between lncRNAs and mRNAs after comparative analysis of their structure and sequence conservation. We found that the upregulated lncRNAs outnumbered the downregulated lncRNAs in glial precursor cells cultured with proBDNF antiserum compared with the levels in glial precursor cells cultured without proBDNF antiserum. By a series of GO and KEGG analyses, we found that the effects of some lncRNAs on their target genes in cis were related to nerve growth factor-induced cell cycle, cell phenotype change, and neuronal differentiation. The qRT-PCR verification results of lncRNAs ENSRNOT00000091991, ENSRNOT00000087717, and LNC_000429 were mostly consistent with the sequencing results. The candidate lncRNAs may be associated with the neuronal transdifferentiation of glial precursor cells. Our study provides the first evidence for a remarkably diverse pattern of lncRNA expression during neuronal differentiation of glial precursor cells from rat DRG, and also provides a resource for lncRNA studies in the field of cell differentiation.