HL-60 세포 분화유도증 c-Myc 발현에 대한 세포분화유도제의 영향

이용진,곽상태,김계영,이명선,권기량,임규,황병두 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.2

Effects of the differentiation inducers on DNA synthesis and c-myc gene expression have been investigated during the differentiation of human promyelocytic leukemia cell line HL-60. All-trans retinoic acid(retinoic acid) and dimethyl sulfoxide(DMSO) decreased DNA synthesis at 24 hours and 12-O-tetradecanoylphorbol 13-acetate(TPA) decreased at 12 hours after exposure, respectively. On the other hand control, no adding differentiation inducers, gradually increased DNA synthesis. The c-myc mRNA was sharply reduced at 24 hours in retinoic acid-, at 4 hours in DMSO-, and at 1 hour in TPA-exposured HL- 60 cells. The level of c-myc mRNAs was reduced in proportion to the concentration in all of three differentiation inducers. The level of c-myc mRNAs in the differentiation inducers-exposured cells were elevated by cycloheximide treatment. These results suggest that c-myc gene expression and differentiation mechanism are variable according to the kinds of the differentiation inducers in HL-60 cells.

All-Trans Retinoic Acid에 의한 HL-60 세포 분화유도중 H2B Histone, c-Myc 및 DNA Topoisomerase I 발현에 관한 연구

임규,박정동,최병한,이용진,김계영,이명선,장은미,김삼용,권기량,곽상태,황병두 忠南大學校 癌共同硏究所 1998 癌共同硏究所 硏究誌 Vol.2 No.1

Effects of all-trans retinoic acid(retinoic acid) on DNA replication, H2B histone and DNA topoisopmerase I(Topo I) gene expression have been investigated in human promyelocytic leukemia cell line HL-60. DNA synthesis decreased at 24 hours after exposure of retinoic acid in the HL-60 cells. H2B histone mRNA rapidly reduced at 48 hours and Topo I and c-myc mRNA at 24 hours in retinoic acid-exposured HL-60 cells, respectively. The levels of c-myc, H2B histone and Topo I gene expression were reduced in proportion to the concentration of retinoic acid. The H2B histone mRNA in retinoic acid-exposured cells was elevated by cycloheximide treatment, while the level of Topo I mRNA was constant. These results suggest that regulations of H2B histone, c-myc and Topo I gene expression are different from one another, and repression of Topo I gene is closely correlated with c-myc gene during retinoic acid-induced differentiation of HL-60 cells.

Comparison of In-Field Measurements of Nitrogen and Other Soil Properties with Core Samples

Kweon, Gi-Young,Lund, Eric,Maxton, Chase,Kenton, Dreiling Korean Society for Agricultural Machinery 2011 바이오시스템공학 Vol.36 No.2

Several methods of in-field measurements of Nitrogen and other soil properties using cores extracted by a hydraulic soil sampler were evaluated. A prototype core scanner was built to accommodate Veris Technologies commercial Vis-NIRS equipment. The testing result for pH, P and Mg were close to RPD (Ratio of Prediction to Deviation = Standard deviation/RMSE) of 2, however the scanner could not achieve the goal of RPD of 2 on some other properties, especially on nitrate nitrogen ($NO_3$) and potassium (K). In situ NIRS/EC probe showed similar results to the core scanner; pH, P and Mg were close to RPD of 2, while $NO_3$ and K were RPD of 1.5 and 1.2, respectively. Correlations between estimations using the probe and the core scanner were strong, with $r^2$ > 0.7 for P, Mg, Total N, Total C and CEC. Preliminary results for mid-IR spectroscopy showed an $r^2$ of 0.068 and an RMSE for nitrate (N) of 18 ppm, even after the removal of calcareous samples and possible N outlier. After removal of calcareous samples on a larger sample set, results improved considerably with an $r^2$ of 0.64 and RMSE of 6 ppm. However, this was only possible after carbonate samples were detected and eliminated, which would not be feasible under in-field measurements. Testing of $NO_3$ and K ion-selective electrodes (ISEs) revealed promising results, with acceptable errors measuring soil solutions containing nitrate and potassium levels that are typical of production agriculture fields.

Soil Profile Measurement of Carbon Contents using a Probe-type VIS-NIR Spectrophotometer

Kweon, Gi-Young,Lund, Eric,Maxton, Chase,Drummond, Paul,Jensen, Kyle Korean Society for Agricultural Machinery 2009 바이오시스템공학 Vol.34 No.5

An in-situ probe-based spectrophotometer has been developed. This system used two spectrometers to measure soil reflectance spectra from 450 nm to 2200 nm. It collects soil electrical conductivity (EC) and insertion force measurements in addition to the optical data. Six fields in Kansas were mapped with the VIS-NIR (visible-near infrared) probe module and sampled for calibration and validation. Results showed that VIS-NIR correlated well with carbon in all six fields, with RPD (the ratio of standard deviation to root mean square error of prediction) of 1.8 or better, RMSE of 0.14 to 0.22%, and $R^2$ of 0.69 to 0.89. From the investigation of carbon variability within the soil profile and by tillage practice, the 0-5 cm depth in a no-till field contained significantly higher levels of carbon than any other locations. Using the selected calibration model with the soil NIR probe data, a soil profile map of estimated carbon was produced, and it was found that estimated carbon values are highly correlated to the lab values. The array of sensors (VIS-NIR, electrical conductivity, insertion force) used in the probe allowed estimating bulk density, and three of the six fields were satisfactory. The VIS-NIR probe also showed the obtained spectra data were well correlated with nitrogen for all fields with RPD scores of 1.84 or better and coefficient of determination ($R^2$) of 0.7 or higher.

비탈면에 적용된 폐지식생기반재의 물리화학적 특성 및 생육특성

정지영(Ji Young Jung),하시영(Si Young Ha),김철환(Chul Hwan Kim),박한민(Han-Min Park),권기영(Gi Young Kweon),양재경(Jae-Kyung Yang) 한국펄프·종이공학회 2018 펄프.종이기술 Vol.50 No.1

In this study, the potential of applying slope surface vegetation material is investigated by manufacturing the physico-chemical properties and growth properties over time after application of waste paper-derived growth media on the slop surface, and by evaluating the soil quality index. The growing media were prepared using different substrate, such as sawdust and waste paper, to grow Lespedeza bicolor on the slope surface (40°). Seed germination of Lespedeza bicolor was found to be 17.5%, 5.8%, 13.8%, 20.8% and 31.5% for the soil (control), sawdust-growing media (100%), waste paper-growing media (100%), soil + sawdust-growing media mixture (50%+50%) and soil + waste paper-growing media mixture (50%+50%), respectively. Seed germination and stem length in the soil + waste paper-growing media mixture (50%+50%) was higher than those in soil (control), sawdust-growing media (100%), waste paper-growing media (100%), or soil + sawdustgrowing media (50%+50%). However, the soil quality index was the highest at 97.4 in waste paper-growing media (100%). This suggests that further study on the soil quality index calculation model on the surface of slope is needed.

Changes in Characteristics of Patients with Liver Cirrhosis Visiting a Tertiary Hospital over 15 Years: a Retrospective Multi-Center Study in Korea

Jang Won Young,정우진,장병국,황재석,이헌주,Hwang Moon Joo,Kweon Young Oh,Tak Won Young,Park Soo Young,Lee Su Hyun,Lee Chang Hyeong,Kim Byung Seok,Kim Si Hye,Suh Jeong Ill,Park Jun Gi 대한의학회 2020 Journal of Korean medical science Vol.35 No.29

Background: Liver cirrhosis has become a heavy burden not only for patients, but also for our society. However, little is known about the recent changes in clinical outcomes and characteristics of patients with cirrhosis-related complications in Korea. Therefore, we aimed to evaluate changes in characteristics of patients with liver cirrhosis in Daegu-Gyeongbuk province in Korea over the past 15 years. Methods: We retrospectively reviewed the medical records of 15,716 liver cirrhotic patients from 5 university hospitals in Daegu-Gyeongbuk province from 2000 to 2014. The Korean Standard Classification of Diseases-6 code associated with cirrhosis was investigated through medical records and classified according to the year of first visit. Results: A total of 15,716 patients was diagnosed with cirrhosis. A number of patients newly diagnosed with cirrhosis has decreased each year. In 2000, patients were most likely to be diagnosed with hepatitis B virus (HBV) cirrhosis, followed by alcoholic cirrhosis. There was a significant decrease in HBV (P < 0.001), but alcohol, hepatitis C virus (HCV), and non- alcoholic fatty liver disease (NAFLD) showed a significant increase during the study period (alcohol, P = 0.036; HCV, P = 0.001; NAFLD, P = 0.001). At the time of initial diagnosis, the ratio of Child-Turcotte-Pugh (CTP) class A gradually increased from 23.1% to 32.9% (P < 0.001). The most common cause of liver-related hospitalization in 2000 was hepatocellular carcinoma (HCC) (25.5%); in 2014, gastrointestinal bleeding with esophageal and gastric varices (21.4%) was the most common cause. Cases of hospitalization with liver-related complication represented 76.4% of all cases in 2000 but 70.9% in 2014. Incidence rate of HCC has recently increased. In addition, HCC-free survival was significantly lower in CTP class A than in classes B and C. Finally, there was significant difference in HCC occurrence according to causes (P < 0.001). HBV and HCV cirrhosis had lower HCC-free survival than alcoholic and NAFLD cirrhosis. Conclusion: In recent years, the overall number of cirrhosis patients has decreased. This study confirmed the recent trend in decrease of cirrhosis, especially of cirrhosis due to HBV, and the increase of HCV, alcoholic and NAFLD cirrhosis. Targeted screening for at-risk patients will facilitate early detection of liver diseases allowing effective intervention and may have decreased the development of cirrhosis and its complications.

Protective Role of Tissue Transglutaminase in the Cell Death Induced by TNF-α in SH-SY5Y Neuroblastoma Cells

Kweon, Soo-Mi,Lee, Zee-Won,Yi, Sun-Ju,Kim, Young-Myeong,Han, Jeong-A,Paik, Sang-Gi,Ha, Kwon-Soo Korean Society for Biochemistry and Molecular Biol 2004 Journal of biochemistry and molecular biology Vol.37 No.2

Tissue transglutaminase (tTGase) regulates various biological processes, including extracellular matrix organization, cellular differentiation, and apoptosis. Here we report the protective role of tTGase in the cell death that is induced by the tumor necrosis factor $\alpha$ (TNF-$\alpha$) and ceramide, a product of the TNF-$\alpha$ signaling pathway, in human neuroblastoma SH-SY5Y cells. Treatment with retinoic acid (RA) induced the differentiation of the neuroblastoma cells with the formation of extended neurites. Immunostaining and Western blot analysis showed the tTGase expression by RA treatment. TNF-$\alpha$ or $C_2$ ceramide, a cell permeable ceramide analog, induced cell death in normal cells, but cell death was largely inhibited by the RA treatment. The inhibition of tTGase by the tTGase inhibitors, monodansylcadaverine and cystamine, eliminated the protective role of RA-treatment in the cell death that is caused by TNF-$\alpha$ or $C_2$-ceramide. In addition, the co-treatment of TNF-$\alpha$ and cycloheximide ecreased the protein level of tTGase and cell viability in the RA-treated cells, supporting the role of tTGase in the protection of cell death. DNA fragmentation was also induced by the co-treatment of TNF-$\alpha$ and cycloheximide. These results suggest that tTGase expressed by RA treatment plays an important role in the protection of cell death caused by TNF-$\alpha$ and ceramide.

자궁 경부암에서 골반 임파절 전이의 예측 인자로서 SCC (squamous cell carcinoma antigen) 의 가치에 대한 연구

강기주,오한진,윤만수,설미영,박창수,고형권,염상탁 대한산부인과학회 2000 Obstetrics & Gynecology Science Vol.43 No.3

목적: 자궁경부암에서 치료전 SCC와 임상적 병기, 종양의 크기, 병변의 범위와의 연관성은 이미 밝혀진 바 있고 또 항암요법이나 방사선 치료 시에 SCC치의 감소는 좋은 예후와 관계됨이 밝혀져 있다. 이에 본 연구는 침윤성 자궁경부암의 치료전 SCC치와 골반 임파절 전이, 종양의 크기, 침윤 정도와의 상관성 유무를 밝히고자 시행하였다. 연구방법: 1995년 3월부터 1998년 12월까지 삼성서울병원 산부인과에서 선행항암요법 없이 자궁경부암으로 양측 골반 임파절 절제를 포함한 양측 골반 임파절 절제를 포함한 광범위 자궁적출술을 시행받은 환자중 수술전 SCC치가 검사된 157명의 환자를 대상으로 하였다. 골반 임파절 전이 유무에 따른 치료전 SCC치 차이를 통계적 증명하기 위해 Mann-Whitney U test를 이용하였으며, 치료전 SCC치에 대한 통계적으로 유의한 독립변수를 분석하기 위해 다중회귀분석법을 이용하였다. 결과: 골반 임파절 전이가 없는 125명의 90%에 해당하는 SCC치 2.9 ng/ml를 기준으로 했을때 SCC치 2.9ng/ml 이상의 환자군 중 골반 임파절 전이가 있을 확률은 60.7%로 2.9미만에서 골반 임파절 전이 양성확률보다 5배 이상 차이를 보였다. SCC치와 종양의 크기, 침윤의 깊이, 골반 임파절 전이 유무에 대한 다중회귀분석에서는 종양의 크기(p=0.04)와 골반 임파절 전이(p=0.002)는 유의한 연관성을 보였으며 침윤의 깊이(p=0.31)는 SCC치와 통계적 유의한 연관성을 보이지 않았다. 본 연구에서는 종양의 크기보다 골반 임파절(p$lt;0.01)전이유무가 SCC치와 더 관련 있는 것으로 나타났다. 결론: 자궁경부암에서 치료전 SCC치가 2.9 ng/ml이상의 경우 골반 임파절 전이의 고위험 인자로 생각되며, 명확하게 정해진 환자군의 범위 내에서 연구가 대규모가 이루어진다면 치료전 SCC치의 기준과 그 타당성의 정도가 좀더 구체적으로 제시될 수 있을 것으로 사료된다. Objective: The clinical value of preoperative serum squamous cell carcinoma antigen(SCC) in relation to clinical stage, tumor volume, disease extent and prognosis has already reported in many papers. The aim of this study is to analyse the relationship between preoperative SCC level and pelvic lymph node metastasis. Matrials and Methods: From March 1995 to December 1998, 157 patients who examined pretreatment SCC levels before undergoing radical hysterectomy for squamous cell carcinoma of uterine cervix were included. The effect of pelvic lymph node status on the SCC level was examined by comparing 125 cases with cancer limited uterus or upper vagina and 32 cases with cancer confined to the uterus (including upper vagina) and pelvic lymph node using multivariate analysis. Results: 90% of patients without pelvic lymph node metastasis showed SCC levels of 2.9ng/ml or below. 60.7% of patients with serum SCC level more than 2.9ng/ml exhibited pelvic lymph node metastasis. The marker values exceeding 2.9ng/ml increased risk of nodal metastasis 5 times compared with serum level 2.9ng/ml or below. Multivariate analysis confirmed that the pelvic lymph node metastasis had a large impact on the marker level than did tumor size or depth of stromal infilteration. Conclusion: SCC levels greater than 2.9ng/ml can be considered a high risk zone for nodal metastasis.

사례분석을 통한 Weighted Summation 방식 변압기 Health Index의 성능 평가

박기훈(Gi-Hun Park),오정식(Jeong-Sik Oh),표수한(Soo-Han Pyo),김용현(Young-Hyun Kim),권동진(Dongjin-Kweon),박태식(Tae-Sik Park) 대한전기학회 2019 대한전기학회 학술대회 논문집 Vol.2019 No.7

난소의 경계성 종양 및 침윤성 암종에서 p53 , Rb 유전자 발현과 Apoptosis 에 관한 연구

강기주,윤만수,설미영,하명완,고형권 대한산부인과학회 2000 Obstetrics & Gynecology Science Vol.43 No.3

목적 : 난소의 악성 종양의 발생에서 종양억제 유전자인 p53과 Rb 단백의 발현과 apoptosis의 역할을 규명하고 이들 인자들이 경계성 종양의 악성화에 미치는 영향을 규명하고자 연구를 시행하였다. 연구 방법 : 30예의 양성, 경계성 및 악성 난소 종양에서 면역 조직화학적 염색을 이용하여 p53 및 Rb 단백의 발현을 조사하고 TUNNEL method를 이용하여 대상 조직에서의 apoptosis 형태를 관찰하였다. 결과 : 양성, 경계성 및 악성 난소 종양에서 p53의 발현은 각각 0, 28 및 94 %로 악성 종양에서 발현율이 높았으며, 항암제에 내성을 보였던 경우 (2/3)와 술 후 잔존 종양이 있었던 경우 (4/5) 및 재발의 경우 (2/2)에서도 고도의 발현을 보였다. Rb 단백은 양성 종양에서부터 소실을 보였으며, 경계성 종양과 악성 종양에서 Rb 단백의 소실은 차이를 보이지 않았다. Apoptosis는 양성 종양에 비하여 악성 종양에서 더욱 활발하게 일어남을 보였으며 p53에 의한 apoptosis의 유도 효과를 간접적으로 확인할 수 있었으나 Rb 단백과 apoptosis 사이의 연관성은 찾을 수 없었다. 결론 : p53 및 apoptosis는 난소의 악성 종양과 양성 종양을 구별하는 인자로 이용될 수 있으며, 또한 종양의 재발 등의 예후와 밀접한 관련이 있는 것으로 나타나 난소의 악성 종양의 진행 및 재발 등의 예후를 추측할 수 있는 인자로 유용하게 이용될 수 있을 것으로 생각된다. Objective: The aim of this study is to evaluate the role of tumor suppressor genes, p53 and Rb gene, as well as apoptosis in the carcinogenesis of ovarian epithelial tumors. And the value of these factors as prognostic markers to tell the transformation of borderline tumors to overt carcinomas is also studied. Method: Thirty cases of ovarian epithelial benign and borderline tumors and invasive carcinoma were used and the expression of the p53 protein and Rb gene protein were evaluated by immunohistochemical method. The apoptosis was evaluated by TUNNEL method. Results: Positive rate of p53 expression in benign, borderline and invasive tumors were 0, 28, and 94 %, respectively. And also, p53 was highly expressed in chemoresistant cases (2/3), in residual tumor (4/5) and in recurred cancer (2/2). Rb protein was partly lost in the borderline tumors, but the rate of Rb protein loss in both borderline tumors and invasive carcinomas were similar. Apoptosis were more active in overt carcinomas than in borderline and benign tumors. In borderline tumors, p53 protein was expressed as 28.6% positivity, and apoptosis was expressed as 28.6% negativity, which showed indirectly that there was apoptosis induction effect of p53. In ten cases of invasive carcinomas showing highly expressed p53, apoptosis revealed all positive reaction except 2 cases, and Rb protein revealed variously. This result supported the apoptosis imduction effect of p53, but it was difficult to find the association of expression degree between the two tumor supressor genes Conclusion: In conclusion, the values of p53 is a discriminating factor of malignancy from benign and the expression of p53 is related with clinical aggressivity such as recurrence and residual cancers. Apoptosis are more active in overt carcinoma than in benign & borderline tumor, and in borderline tumor the expression of p53 is related to apoptosis induction which results to carcinomatous change.