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      • KCI등재

        국내 수집 야생버섯류 추출물의 생리활성 비교

        안기홍,한재구,김옥태,조재한,An, Gi-Hong,Han, Jae-Gu,Kim, Ok-Tae,Cho, Jae-Han 한국버섯학회 2021 한국버섯학회지 Vol.19 No.1

        국내 자생하는 야생버섯 추출물의 생리활성 성분을 평가하기 위하여 각지에서 수집된 야생버섯 70% 에탄올추출물에 대한 DPPH 라디컬 소거능, 총 폴리페놀 및 총 플라보노이드 함량, 철 환원 항산화능, 환원력의 항산화 활성, 아질산염 소거능 및 야생버섯의 건조시료를 이용하여 베타글루칸 함량을 분석하였다. 본 연구에서 수집된 10종의 야생버섯류 중에서 영지(OK1362) 에탄올추출물의 DPPH 라디컬 소거능(73.2%), 총 폴리페놀 함량(28.9 mg GAE/g) 및 총 플라보노이드 함량(10.0 mg QE/g)철 환원 항산화능(0.134), 환원력(0.155), 아질산염 소거능(56.3%)이 다른 버섯류에 비하여 유의적으로 가장 높았다. 그 이외에도 아까시흰구멍버섯(OK1360), 광대버섯속의 A. lanigera (OK1398), 졸각무당버섯(OK1406)이 높은 항산화능 및 아질산염 소거능을 가지고 있음을 확인하였다. 또한 베타글루칸 함량은 영지(OK1362)가 25.2%를 나타내며 가장 높았으며, 그 외에 구름버섯속의 T. lactinea (OK1457)가 24.5%로 높은 것으로 나타났다. 본 연구의 결과는 국내 자생하는 야생버섯류 중 새로운 천연물 유래 생리활성 물질을 탐색하기 위한 기초자료로서 활용 가능성을 기대한다. The aim of the study was to obtain the extracts of various native wild mushrooms and select the useful resources though biological activity evaluation. The anti-oxidant potential, nitrite scavenging activity, and ��-glucan content of wild mushrooms collected from Eumseong and Bonghwa in Korea were investigated. Based on the results of this study, Ganoderma lingzhi (OK1362) showed the highest DPPH radical scavenging activity (73.2%), ferric reducing anti-oxidant power (0.134), reducing power (0.155), nitrite scavenging activity (53.6%), total polyphenol content (28.9 mg GAE/g), flavonoid content (10.0 mg QE/g), and ��-glucan content (25.2%) when compared to other wild mushrooms sampled in this study. In addition, it was confirmed that Perenniporia fraxinea (OK1360), Amanita sp. (OK1398), and Russula sp. (OK1406) had relatively high anti-oxidant and nitrite scavenging potentials. In conclusion, our results can provide fundamental data for extracting beneficial compounds from wild mushrooms.

      • KCI등재

        영어 Much of an N 구문에 대한 말뭉치 기반적 접근

        김옥기(Ok gi Kim),김종복(Jong Bok Kim) 현대문법학회 2015 현대문법연구 Vol.86 No.-

        Okgi Kim and Jong-Bok Kim. 2015. English much of an N Construction: A Corpus-based Approach. Studies in Modern Grammar 86, 47-70. English much of an N (henceforth, MOA) construction (e.g., It’s not much of a problem.) consists of quantifiers like much, more/less, enough, little and of-an-NP to intensify the indefinite singular degree NP. In this paper, we first review basic properties of the construction, focusing on the relation between the gradability of the NP and its predicativeness. In particular, after investigating authentic corpus data of the MOA, we provide a qualitative and quantitative analysis which allows a constraint-based approach in accounting for various syntactic as well as semantic properties of the construction.

      • KCI등재
      • 제주먹초파리(Drosophila sp ; Robusta Group)에서 난황 단백질 합성에 미치는 유충 호르몬Ⅲ(Juvenile hormone Ⅲ)의 영향

        김기옥,오문유,김세재 濟州大學校 基礎科學硏究所 1992 基礎科學硏究 Vol.5 No.1

        제주먹초파리 (Drosophila sp: robusta group)에서의 난황 단백질 합성은 우화 후 2일된 female 성체의 지방체와 난소의 여포세포 (follicle cell)에서부터 합성되기 시작했다. 그리고 합성된 난황 단백질은 hemolymph로 방출된 후 선택적으로 난소로 흡수되어 완전히 성숙한 embryo를 생성하는데는 우화 후 약 4일정도가 소요되었다. 난황 단백질 유전자 발현은 곤충 호르몬인 20-hydroxyecdyson(20HE)과 juvenile hormone (JH)에 의하여 조절된다. 미성숙한 female성체에서 복부만을 분리하여 10-6M에서 10-3M의 juvenile hormoneⅢ 을 0.3 ㎕정도 주입하여 난황단백질을 합성을 유도 할수 있었다. 그리고 10-3M의 JHⅢ 처리후 2-3시간 사이에 난황 단백질의 합성을 추적할 수 있었다. 우화 후 2일된 female 성체에 수종의 호르몬 (Acetone, JHⅢ, JHⅢ-20HE)을 처리한 결과 난소의 발달 효과는 JHⅢ 처리구에서 가장 높았다. 따라서 JHⅢ는 vitellogenesis동안에 지방체와 여포세포에 있는 난황 단백질 유전자를 활성화시킴은 물론 난소의 발달에도 많은 영향을 미치는 것으로 보인다. Three yolk proteins of Drosophila sp (robusta group) begin to be synthesized in fat body and ovarian follicle cells of two female after eclosion, and selectively accumulated by the oocyte during vitellogenesis. Expression of the yolk protein genes are regulated by the insect hormone, each as 20-hydroxyecdyson and juvenile hormone. An injection of 0.3 ㎕ of 10-6 M JHⅢ in one day-old female was sufficient to promote synthesis and secretion of yolk proteins in isolated abdomens. Three yolk proteins were first detected between 2 and 3 hrs after treated of 10-3 M JHⅢ in abdomens removed from the whole body of one day-old female flies. The vitellogenin oocytes development by treatment with some hormones (acetone, JHⅢ, and JHⅢ-20HE) was highly affected in JHⅢ of 2-day old female flies after eclosion. Thus it seems like that increase juvenile hormone levels not only switches on yolk protein genes during vitellogenesis but also promotes the oocytes development.

      • 표준물질 조제를 위한 퇴적물 중 유기염소제 농약 및 Poiychlorinated Biphenyls의 분석

        김미옥,이성인,정기호,소헌영 부산대학교 환경문제연구소 2002 環境硏究報 Vol.20 No.-

        Standard reference materials(SRM) have been used to certify the degree of accuracy for determining various kinds of analytes including environmental organic contaminants. In this study, we collected sediments from the Nakdong River estuary and Ulsan Bay. The levels of polychlorinated biphenyls(PCBs) and organochlorine pesticides were determined to investigate the availability of SRM and its homogeneity. The 8 congeners of PCBs and chlordane, p,p'-dichlorodiphenyldichloroethane(DDD), p,p'-dichlorodiphenyldichloroethylene(DDE), p,p'-dichlorodiphenyltrichloroethane(DDT), dieldrin, hexachlorobenzene(HCB) and oxychlordane were determined by using a capillary column gas chromatography/electron capture detector system. The environmental levels of these species md their relative standard deviations are discussed.

      • 화훼식물의 분류와 區系에 관한 연구 : 부산.경남을 중심으로

        이옥자,문성기 慶星大學校 1998 論文集 Vol.19 No.1

        1. Horticulture plants for flowers searched in trading centers of flower and flower growing gardens of Pusan city and Kyungnam province amount to 115kinds and its taxonomical category were 3 divisions, 4 classes, 2 subclasses, 29 orders, 16 suborders, 43 families, 88 genera by Enger classification system. 2. According to the biogeographycal floristic regions of flower horticulture plants in this study Boreal region has 42 kinds(37.5%), Neotropical 31 kinds(21.5%), otherwise South African has 9 kinds(6.3%), Australian 6 kinds(4.2%), Antarctic 2 kinds(1.4%) in order. 3. In general Korean names of 40 kinds of flower horticulture plants were called as genus name of scientific name for example Korean name of "ACHIMENES" come from Achimenes and "ADIANTUM" come from Adiantum.

      • 북한의 토지소유제도

        김기옥 호남대학교 2005 호남대학교 학술논문집 Vol.26 No.1

        Since divided into two Koreas, South and North Korea have been building their own land use system over tears, However, North Korea has been managing its land less effectively than South Korea in land development and uses, resulting in impaired competitive power in the world. Considering future reunification of Korea, barriers which are caused by too different land uses in both countries. However, as some methods that had been done so far just summarized existing information, there are not enough imformation to mend land use system in North Korea. If reunification of Korea occurs, several pending issues such as land ownership, land confiscation by communist party, introduction of real estate, restoration of North Korea's economy, and land intergration will rise in the future.

      • Penna-Stauffer 모형에 있어서의 노화의 스스로 짜임

        김기옥,한영덕,이용현 우석대학교 부설 기초과학연구소 1997 기초과학연구소 논문집 Vol.2 No.-

        The Penna-Stauffer model for biological aging is studied. In our work whether the reproduction of each individual is possible or not was determined by means of the number of mutations relevant at that time. The Penna model shows that the species tend to be fitted by themselves to the direction that maximized the self-conservation. The population, the survival rate, the average life span and the average age were calculated with respect to the number of new mutations 1, 2, and 3. The more the number of new mutations, the shorter the average life span, and the higher the average age.

      • DLA모형에 의해 성장된 구조의 물리적 차원

        김기욱 又石大學校 1988 論文集 Vol.10 No.-

        Using electronic computer a large cluster was grown by DLA model, and several physical dimensions of the structure were calculated by Monte carlo method. It is shown that those values satisfy the relations among them approximately.

      • 벗나무속 수종간의 핵 리보솜 DNA의 ITS Ⅰ 염기서열 비교

        오유성,김기옥,정용환,오문유,한상현,고미희 제주대학교 생명과학연구소 1998 제주생명과학연구 Vol.1 No.-

        Comparison of four species (Prunus yedoensis, P. sargentii, P. pendula for. ascendens, P. serrulata var. quelpaertensis) and one cultivar of P. yedoensis were carried out by comparing their nucleotide sequences from the internal transcribed spacers (ITS) I of nuclear ribosomal DNA (rDNA). The length of the ITS I region was ranged from 229 to 236 base pairs (bp). The G+C contents of ITS I ranged from 58.5 to 60.2%. The value of nucleotide divergence across for four Prunus species and one cultivar ranged from 0.4 to 6%. The pairwise distance between P. pendula for. ascendens and P. yedoensis was 0.004 showing the lowest value among any other pairs. On the other hand, the pairwise distance betwen P. yedoensis and cultivated P. yedoensis was 0.026. The nucleotide analysis based on ITS I sequence was considerably similar to previous studies on the basis of random amplified polymorphic DNA (RAPD).

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