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김은경 ( Eun-gyeong Kim ),김민경 ( Min-kyung Kim ),권현애 ( Hyun-ae Kwon ),윤도경 ( Do-kyung Youn ),구정헌 ( Jeong-heon Koo ),박소연 ( So-yeon Park ),이희근 ( Hui-geun Lee ),조명희 ( Myeong-hui Jo ),하도윤 ( Do-yun Hah ),김철호 ( 한국동물위생학회(구 한국가축위생학회) 2018 韓國家畜衛生學會誌 Vol.41 No.3
Salmonella Enteritidis (S. Enteritidis) are found in animals, humans, and environment. In addition, S. Enteritidis draws attention to the public health concerns due to carriage of antibiotic resistance traits. For these reasons, the prevalence and antibiotic resistance patterns of S. Enteritidis are significant issues with regard to public health. To address this issues, a total of 24 strains of S. Enteritidis from 164 samples collected from several slaughterhouses in Gyeong-Nam province in order for antibiotic resistance profiles. Subsequently, we characterized the genotyping by random amplification polymorphic DNA (RAPD)-PCR. As a result, very high level of resistance to protein synthesis inhibition antibiotics and most isolates were susceptible to others. Six random primers were used for RAPD-PCR to reveal genotypes of S. Enteritidis isolates. One of the primer, P1245, generated 147 distinct RAPD-PCR fragments ranging from 400∼3000 bp. The number of RAPD-PCR products ranged from 4 to 8 for this primer. The RAPD-PCR fragments could be placed these strains into 3 subgroups and 2 classes by UPGMA cluster analysis. Interestingly, several S. Enteritidis that isolated from different slaughterhouses showed same genotype. These results showed only limited genetic variation among the isolates, those were grouped into a few different patterns of antibiotic resistance.
李乙熙,崔相道,吳錫斗,高鏡畢 진주산업대학교 농업기술연구소 1991 農業技術硏究所報 Vol.4 No.-
아미노산과 5炭糖을 121℃에서 15분간 열처리하여 Maillard반응의 發色强度를 비교한 결과는 다음과 같다. 1. 아미노산은 發色强度가 높은것, 중간것, 낮은것으로 대별되었다. 2. 糖은 L(+)arabinose, D(-)-ribose, D(+)-xylose 순으로 發色强度가 높게 나타났다. 3. pH차이에 따른 영향은 反應液의 pH가 높아질수록 反應性은 增加되었다. 4. D(+)-xylose, D(-)-ribose 및 L(+)-arabinose의 농도가 높아질 수록 반응성은 높게 나타났다. Common amino acids and pentoses have been ranked acording to the intensity of Maillard browning formed when heated in an autoclave at 121℃ for 15 min. The results obtained as follows; 1. The amino compounds have been groupeed into high, intermediate and low browning producing groups. 2. The color intensity of pentoses tested was xylose>ribose>arabinose. 3. As the pH values of reaction solutions increased, the color intensity of melanoidins. 4. As the concentrations of xylose, ribose and arabinose increased, the color intensities of melanoidins.
( Do Gyeong Lee ),( Ji Hae Park ),( Ki Hyun Yoo ),( In Sik Chung ),( Youn Hyung Lee ),( Jong Ki Lee ),( Dae Seok Han ),( Sueng Mock Cho ),( Nam In Baek ) 한국응용생명화학회 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.2
Fucosterol (2), oxygenated fucosterol, 24-ethylcholesta-4,24(28)-dien-3,6-dione (3), β-sitosterol (1), and daucosterol (4) were isolated from ethyl acetate (EtOAc) fraction of Osmanthus fragrans var. aurantiacus flower by repeated silica gel, octadecyl silica gel, and Sephadex LH-20 column chromatography. The sterol structures were determined based on spectroscopic methods including nuclear magnetic resonance, infrared spectroscopy, fast atom bombardment mass spectrometry, and electron ionization mass spectrometry. Fucosterol (2), oxygenated fucosterol, and 24-ethylcholesta-4,24(28)-dien-3,6-dione (3) are representative sterols mainly isolated from marine algae, but rarely found in land plants. This is the first reported isolation of these sterols from O. fragrans var. aurantiacus. Interestingly, the oxygenated fucosterol (3) isolated inhibited the in vitro growth of the HCT-116 human colon cancer cell line with higher cytotoxic effects than doxorubicin, a well-known cytotoxic drug.
Do Gyeong Lee,Jin Sung Choi,Seung Woo Yeon,En Ji Cui,Hee Jung Park,Jong Su Yoo,In Sik Chung,Nam In Baek 한국응용생명화학회 2010 Journal of Applied Biological Chemistry (J. Appl. Vol.53 No.3
One secoiridoid glycoside was isolated from the EtOAc-soluble fraction of flowers of Osmanthus fragrans var. aurantiacus by repeated silica gel, octadecyl silica gel (ODS) and Sephadex LH-20 column chromatographies. It was identified as (8E)-ligstroside ba
Lee, Do-Gyeong,Choi, Jin-Sung,Yeon, Seung-Woo,Cui, En-Ji,Park, Hee-Jung,Yoo, Jong-Su,Chung, In-Sik,Baek, Nam-In The Korean Society for Applied Biological Chemistr 2010 Applied Biological Chemistry (Appl Biol Chem) Vol.53 No.3
One secoiridoid glycoside was isolated from the EtOAc-soluble fraction of flowers of Osmanthus fragrans var. aurantiacus by repeated silica gel, octadecyl silica gel (ODS) and Sephadex LH-20 column chromatographies. It was identified as (8E)-ligstroside based on spectroscopic methods including NMR and MS. This compound has been first isolated from Osmanthus fragrans var. aurantiacus, and showed BACE1 inhibition activity.
Do-Gyeong Lee,박지해,유기현,정인식,이윤형,Jong-Ki Lee,Dae-Seok Han,조승목,Nam-In Baek 한국응용생명화학회 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.2
Fucosterol (2), oxygenated fucosterol, 24-ethylcholesta-4,24(28)-dien-3,6-dione (3), β-sitosterol (1), and daucosterol (4) were isolated from ethyl acetate (EtOAc) fraction of Osmanthus fragrans var.aurantiacus flower by repeated silica gel, octadecyl silica gel, and Sephadex LH-20 column chromatography. The sterol structures were determined based on spectroscopic methods including nuclear magnetic resonance, infrared spectroscopy, fast atom bombardment mass spectrometry, and electron ionization mass spectrometry. Fucosterol (2), oxygenated fucosterol, and 24-ethylcholesta-4,24(28)-dien-3,6-dione (3) are representative sterols mainly isolated from marine algae, but rarely found in land plants. This is the first reported isolation of these sterols from O.fragrans var. aurantiacus. Interestingly, the oxygenated fucosterol (3) isolated inhibited the in vitro growth of the HCT-116 human colon cancer cell line with higher cytotoxic effects than doxorubicin, a well-known cytotoxic drug.
Lee, Min-Kyung,Park, Hee-Jung,Kwon, Sung-Ho,Jung, Ye-Jin,Lyu, Ha-Na,Lee, Do-Gyeong,Song, Na-Young,Choi, Hong-Keun,Park, Sangkyu,Baek, Nam-In,Lee, Youn-Hyung 한국응용생명화학회 2013 Applied Biological Chemistry (Appl Biol Chem) Vol.56 No.1
Principal components of aquatic plants responsible for inhibiting the growth of Microcystis aeruginosa, a blue-green alga (cyanobacterium) were determined. Methanol extracts of Brasenia schreberi showed strong inhibitory activity against the growth of the cyanobacterium. Two purified flavonoids were afforded through repeated column chromatographies. The compounds were determined as quercetin (1) and quercetin 3-O-(6"-galloyl)-${\beta}$-D-glucopyranoside, tellimoside (2) by the interpretation of spectroscopic data including NMR, MS, and IR. Compounds 1 and 2 were first isolated from Brasenia schreberi. In addition, compound 2 showed very strong inhibitory activity against the growth of M. aeruginosa.