Dysregulated Fatty Acid Metabolism in Hepatocellular Carcinoma

( Ming-da Wang ),( Jun Han ),( Hao Xing ),( Han Zhang ),( Zheng Wang ),( Zhen-li Li ),( Liang Lei ),( Chao Li ),( Feng Shen ),( Tian Yang ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1

Aims: Studies are urgently needed on it molecular pathogenesis and biological characteristics of hepatocellular carcinoma (HCC). Dysregulation of fatty acid (FA) metabolism, in which aberrant activation of oncogenic signaling pathways alters the expression and activity of lipid-metabolizing enzymes, is an emerging hallmark of cancer cells, and it may be involved in HCC development and progression. Methods: We summarize the characteristics of FA metabolism in HCC, focusing on the pathways of FA synthesis, oxidation, uptake and transport. We also provide a brief review of the relationship between NAFLD and HCC development. Results: The current review summarizes the dysregulated FA metabolism in HCC and pathways through which this dysregulation may regulate HCC survival and growth. Aberrant activation of oncogenic signaling pathways regulates the expression and activity of lipid-metabolizing enzymes, thus reprogramming FA metabolism to promote HCC development and progression. Intracellular FAs are required for biosynthesis of most biological membrane lipids and signaling molecules, and are also used to provide energy to support HCCs survival and proliferation, when necessary, through β-oxidation process. HCC cells can employ appropriate metabolic pathways as different situation demands. Intrahepatic cholangiocarcinoma (ICC) and HCC exhibits differential requirement for de novo lipogenesis and distinct response to therapeutic approaches focusing on inhibition of exogenous FA uptake. Non-alcoholic fatty liver disease related obesity and diabetes have increasingly emerged as two major factors responsible for the rise in prevalent of HCC. Conclusions: Our understanding of dysregulated FA metabolism and associated signaling pathways may contribute to the development of novel and efficient anti-tumor approaches for patients with HCC.

港口水域溢油回收技術和系統裝置的硏究和開發

서달봉(Xu Da feng),장정비(Zhang Zheng Fei) 한국항만경제학회 1994 韓國港灣經濟學會誌 Vol.10 No.-

This article introduces some new research results and series products for control oil spill pollution on water area developed by Qindao Guangmin Research Institute of Applied Technology. The Series recovery equipment includes of different Oil boom, Oil Skimmer, FastTand, Floatig Oil Bay and other necessary supplementary installations.<br/> Their general performance has reached the international advanced level of same type products of 1980s. And it has gained the Silver Medal in "the 3th National New Technique and New Products Exhibition" held by National Science &. Technology Committee. and be sent to San Panlo Brazil to join "the Whole World Environmental Protection Develop International Exhibition".

Fast Implementation of the Progressive Edge-Growth Algorithm

Lin Chen,Da-Zheng Feng 한국전자통신연구원 2009 ETRI Journal Vol.31 No.2

A computationally efficient implementation of the progressive edge-growth algorithm is presented. This implementation uses an array of red-black (RB) trees to manage the layered structure of check nodes and adopts a new strategy to expand the Tanner graph. The complexity analysis and the simulation results show that the proposed approach reduces the computational effort effectively. In constructing a low-density parity check code with a length of 104, the RB-tree-array-based implementation takes no more 10% of the time required by the original method.

Cytotoxic anthraquinone dimers from Melandrium firmum

Chang Hao Zhang,Da Lei Yao,Cheng-Shen Li,Jie Luo,Mei Jin,Ming-Shan Zheng,Zhen-Hua Lin,Tie-Feng Jin,Gao Li 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.6

Two new anthraquinone dimers, melrubiellin A(1) and melrubiellin B (2), were isolated from the aerialpart of Melandrium firmum Rohrbach, along with sevenknown compounds (3–9). The structures of these compoundswere elucidated by spectral analyses, including 1Dand 2D NMR (COSY, HMQC, HMBC and NOESY)experiments. Compound 1 and 2 exhibited significantcytotoxicity towards HeLa, NCI-H460, Hep G2, Hep 3Band MKN-28 cell lines with IC50 values ranging from 5.26to 81.16 lM.

Enhancement clay tolerance of PCE by lignin-based polyoxyethylene ether in montmorillonite-contained paste

Xiao-Kang Li,Da-Feng Zheng,Tao Zheng,Xu-Liang Lin,Hong-ming Lou,Xueqing Qiu 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.49 No.-

The dispersion ability of polycarboxylate ether (PCE) in fresh concrete is much impeded by clayimpurities. To improve the dispersion of PCE in the clay-contained concrete, lignin-based polyoxy-ethylene ether (PEG-grafted-lignin) was synthesized through polyethylene glycol (PEG) grafted to kraftlignin (KL). The results showed that PEG-grafted-lignin increased thefluidity of MMT-contained cementpaste when combined with PCE. It improved the rheological properties of MMT-contained cement pasteby reducing the yield stress and the rheological behavior index. The adsorption of PEG-grafted-lignin onMMT was more quickly than KL and the equilibrium adsorption amount was 31.43 mg/g at an initialconcentration of 100 mg/L. XRD revealed that PEG-grafted-lignin had inserted into the interlayerstructure of MMT, while DLS disclosed the spatial effect of PEG-grafted-lignin was much stronger thanPCE. This study put forward a new method in the anti-sludge study of PCE.

UV-ARTP-DES compound mutagenesis breeding improves natamycin production of Streptomyces natalensis HW-2 and reveals transcriptional changes by RNA-seq

Jianrui Sun,Jinglan Li,Linlin Yao,Yingying Zheng,Jiang-Feng Yuan,Da-Hong Wang 한국식품과학회 2023 Food Science and Biotechnology Vol.32 No.3

Natamycin is widely used in food, medical and health, agriculture, and animal husbandry. In this study, Streptomyces natalensis HW-2 was used as the research object, and a mutant DES-26 with stable genetic characters was selected by UV-ARTP-DES compound mutation. The natamycin yield was 1.64 g/L, 86.36% higher than original strain. Differential expression genes were analyzed by transcriptomics, and results showed that 295 and 860 genes were significantly differentially expressed at fermentation for 48 h and 72 h. GO and KEGG analysis showed that compound mutagenesis had a significant impact on glycolysis, pentose phosphate, TCA cycle, fatty acid metabolism pathways, and several key enzyme genes in the pathways were up-regulated, and genes related to natamycin biosynthesis (pimB-pimI) and transcriptional regulator (pimR) were also up-regulated. qRT-PCR results confirmed that expression levels of these genes were consistent with transcriptional changes of RNA-Seq.

Highly Oriented Monolayer Graphene Grown on a Cu/Ni(111) Alloy Foil

Huang, Ming,Biswal, Mandakini,Park, Hyo Ju,Jin, Sunghwan,Qu, Deshun,Hong, Seokmo,Zhu, Zhili,Qiu, Lu,Luo, Da,Liu, Xiaochi,Yang, Zheng,Liu, Zhongliu,Huang, Yuan,Lim, Hyunseob,Yoo, Won Jong,Ding, Feng,Wa American Chemical Society 2018 ACS NANO Vol.12 No.6

<P>Fast-growth of single crystal monolayer graphene by CVD using methane and hydrogen has been achieved on “homemade” single crystal Cu/Ni(111) alloy foils over large area. Full coverage was achieved in 5 min or less for a particular range of composition (1.3 at.% to 8.6 at.% Ni), as compared to 60 min for a pure Cu(111) foil under identical growth conditions. These are the bulk atomic percentages of Ni, as a superstructure at the surface of these foils with stoichiometry Cu<SUB>6</SUB>Ni<SUB>1</SUB> (for 1.3 to 7.8 bulk at.% Ni in the Cu/Ni(111) foil) was discovered by low energy electron diffraction (LEED). Complete large area monolayer graphene films are either single crystal or close to single crystal, and include folded regions that are essentially parallel and that were likely wrinkles that “fell over” to bind to the surface; these folds are separated by large, wrinkle-free regions. The folds occur due to the buildup of interfacial compressive stress (and its release) during cooling of the foils from 1075 °C to room temperature. The fold heights measured by atomic force microscopy (AFM) and scanning tunneling microscopy (STM) prove them to all be 3 layers thick, and scanning electron microscopy (SEM) imaging shows them to be around 10 to 300 nm wide and separated by roughly 20 μm. These folds are always essentially perpendicular to the steps in this Cu/Ni(111) substrate. Joining of well-aligned graphene islands (in growths that were terminated prior to full film coverage) was investigated with high magnification SEM and aberration-corrected high-resolution transmission electron microscopy (TEM) as well as AFM, STM, and optical microscopy. These methods show that many of the “join regions” have folds, and these arise from interfacial adhesion mechanics (they are due to the buildup of compressive stress during cool-down, but these folds are different than for the continuous graphene films-they occur due to “weak links” in terms of the interface mechanics). Such Cu/Ni(111) alloy foils are promising substrates for the large-scale synthesis of single-crystal graphene film.</P> [FIG OMISSION]</BR>

Is Surgical Resection Justified for Hepatocellular Carcinoma with Portal Vein Tumor Thrombus? (A Systematic Review and Meta-Analysis)

( Liang Lei ),( Xin-fei Xu ),( Jiong-jie Yu ),( Ju-dong Li ),( Zhen-li Li ),( Jun Han ),( Han Zhang ),( Hao Xing ),( Han Wu ),( Ming-da Wang ),( Chao Li ),( Zheng Wang ),( Feng Shen ),( Meng-chao Wu ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1

Aims: The prognosis of hepatocellular carcinoma (HCC) with portal vein tumor thrombus (PVTT) is very poor. According to the BCLC treatment recommendations, sorafenib or other palliative treatment (PT) is recommended as the first-line therapy when it happens. In real world, however, a significant number of selected patients with HCC and PVTT suffered from surgical resection (SR). Methods: PubMed, Embase, Medline and Cochrane library were searched for studies comparing SR with PT (including TACE, sorafenib, etc.) for HCC with PVTT, which were published before September 2017. Results: 4,810 patients from 7 studies were enrolled in this meta-analysis, which divided into the SR group (n = 2,344) and the PT group (n = 2476). When compared with the PT group, the pooled hazard ratio (HR) for the 1, 3 and 5-year OS rates of the SR group were 0.56 (95% CI 0.52-0.60, P=0.03), 0.56 (95% CI 0.53-0.59, P<0.001) and 0.55 (95% CI 0.54-0.57, P<0.001). For subgroup analysis, when compared with the mere TACE group, the pooled HR for the 1, 3 and 5-year OS rates of the SR group were 0.54 (95% CI 0.43-0.67, P=0.81), 0.75 (95% CI 0.65-0.87, P=0.25) and 0.76 (95% CI 0.67-0.88, P=0.25). Conclusions: This meta-analysis demonstrated SR had better OS than TACE or other palliative therapy for HCC with PVTT. SR may be suitable as the first-line treatment for selected patients with resectable HCC and removable PVTT.