AH-66 Hepatoma 세포의 Histone에 관한 연구

최종오,이동욱,이희성 중앙대학교 의과대학 의과학연구소 1985 中央醫大誌 Vol.10 No.2

In attempt to investigate histone fractions of tumor cells, histone fractions HI, H2a, H2b, H3 and H4 were prepared from isolated nuclei of AH-66 hepatoma cells and histone fractions were characterized by amino acid analysis and by polyacrylamide disc gel electrophoresis. The results obtained were as follows; 1, The five histone fractions found in most tissues were also present in the AH-66 hepatoma cell histories. 2. The yield of whole histone was 6.46mg per g of packed cells. 3. The yield of DNA was 14.78mg per g packed cells. Consequently the DNA to histone ratio was 1:0.44. 4. The relative amounts of five fractions, i.e., H1, H2a, H2b, H3 and H4 were 15.64% 34.83%, 21,21%, 11.76% and 16.56%, respectively. The data presented in this report show that there is a considerable variation in the relative amounts of histone fractions as compared to those of calf thymus. 5. The electrophoretic mobility of individual histone fractions gave almost similar patterns to those of corresponding fractions of calf thymus. 6. Amino acid analysis of the individual histone fractions showed that the over-all compositions were similar but not identical to those of corresponding fractions from calf thymus and other tumor cells. 7. Histone fractions HI, H2b. H3 and H4 were found to contain detectable amounts of ε-N-monomethyllysine. Usually the methylated derivatives of histidine have been reported in histone Hi fraction of Sarcoma 180 ascites tumor cells and Walker 256 carcinosarcoma. However, the methylated histidine residue was not found in all histone fractions from AH-66 hepatoma cells. 8. The evidence was presented that AH-66 hepatoma cells contained five major histone fractions which were homologous to corresponding five fractions of calf thymus.

담양호, 광주호 유역의 삼림생태계에서 중금속 함량 비교

崔英燮,曺熙枓 全南大學校 農業科學技術硏究所 1998 農業科學技術硏究 Vol.33 No.-

본 연구는 광주에서 약 25km 떨어져 있는 담양호와 광주시에 인접해 있는 광주호의 삼림유역에서 수목이 중금속을 흡수하는 양을 파악하기 위하여 소나무와 굴참나무의 엽과 뿌리, 삼림토양의 단면 중에서 L층, F층, A층에 함유된 중금속 함량과 강우, 호소수, 하천수의 중금속 함량을 ICP-AES에 의하여 분석하였다. 본 연구에서는 중금속중에서 대기와 토양오염과 밀접한 관련이 있고 동·식물의 생장 및 생육에 영향을 미치는 Mo, Zn, Pb, Ni, Cd, Mn, Cr, Cu로 제한하여 측정 분석하여 얻은 결론은 다음과 같다. 1. 생장초기의 중금속 함량은 각각 중금속이 미량으로 나타나며 원소에 따라 약간의 차이는 있으나 Mn과 Pb는 삼림토양 단면(L, F, A층)에서 높게 나타났다. 2. 생장말기의 중금속 함량은 각각 중금속이 미량으로 나타나는데 Mn과 Pb는 삼림토양 보다는 수목에서 높게 나타났는데 이는 토양에 함유된 Mn, Pb를 흡수하여 수목의 뿌리나 잎에 축적되었기 때문이다. 따라서 낙엽인 L 층에도 Mn과 Pb가 다량 함유되어 있다. 3. 뿌리를 통해서 흡수된 중금속은 잎에 집적되었다가 낙엽으로 다시 토양에 공급되는데 토양에 공급된 중금속과 토양생성과정에서 모암에서 생성된 중금속이 강우에 의하여 호수에 유입된다. 따라서 본 연구에서는 5월과 9월에 담양호와 광주호의 호소수에 중금속의 함량이 적은 것은 호소수가 하천수로 유출되어서 희석된 결과라고 사료된다. 4. 강우 역시 5월과 9월에 담양호와 광주호 삼림유역에서 중금속의 함량에 큰 차이가 없는 것은 광주시에 인접되어 있는 광주호가 도시매연이나 자동차 배기가스 등 오염물질의 영향을 받지 않은 결과라고 사료된다. 5. 생장초기는 삼림토양에 다량 축적된 중금속류가 생장말기에 이르러서는 수목의 잎과 뿌리로 다량 흡수함을 알 수 있는데 강우에 중금속류가 소량 측정되어 대기오염물질이 엽을 통한 직접 흡수보다는 뿌리를 통해 엽으로 이동된 것으로 생각된다. 6. 광주호 삼림유역은 광주시에 인접해 있으므로 도시매연이나 자동차 배기가스 등의 영향을 많이 받아 강우나 호소수, 수목의 뿌리, 잎, 토양단면에 중금속이 담양호 삼림유역보다 더 많이 측정되리라고 생각하였으나, Mn과 Pb에서 생장초기에는 담양호 삼림유역의 소나무의 잎과 뿌리에서 더 많이 측정되었고, 생장말기에는 광주호 삼림유역의 굴참나무의 잎과 뿌리에서 높게 나타났다. This study was to compare the concentrations of heavy metals absorbed in Pinus densiflora with those absorbed in Quercus variabilis, by analysing with ICP-AES the concentrations of heavy metals in leaves and fine roots of both trees, the concentrations in L layer, in F layer, and in A horizon of soil profile, and the concentrations in rainfall, in lake water and in streams in the above both forest ecosystem of the Tamyang lake and Kwangju lake watershed. The selected heavy metals were Mo, Zn, Pb, Ni, Cd, Mn, Cr, Cu, closely related with the air pollution and soil contamination, and essential for the growth of both plants and animals. the samples were collected at the early period of growth on may, 1997 and at the closing period of growth on september, 1997. 1. In early period of growth, the concentrations of Mn, Pb were very much higher than those of others in L, F layer and A horizons of soils profile. But in the closing period of growth, the amounts of Mn, Pb were included more in the trees than in the soils. This was considered as the results of accumulating in the tree tissues after absorbing the heavy metals from soils. But very little contents of other heavy metals were included in the profile and in the trees in the early period or in the closing period of growth. 2. The movement of heavy metals cycled from the soils to the plants through the absorption of the metals with other nutrients and adversely from the fallen leaves of plants to the soils. The heavy metals derived from the plant residues and the parent rocks put in the lake and stream by rainfall. The concentration of heavy metals generally were in low both lake water of Tamyang lake and Kwangju lake, because both lake water were diluted by rain water or stream water. 3. There was no great difference in the concentrations of heavy metals included in rainfall of both Tamyang lake and Kwangju lake watershed and there was no any evidence affected to the both areas by the atmospheric pollution of Kwangju city.

양측 신결석과 혈관기형을 동반한 마제철신 1례

최홍용,노충희,최희석 인제대학교 1986 仁濟醫學 Vol.7 No.3

A case of horseshoe kidney associated with bilateral renal stones and variation of the renal vessel was presented with a brief review of the literatures. The patient was managed by ureterolithotomy and ureteroureterostomy because UPJ was compressed with aberrant renal vein. The result was good.

김동인의「雲峴宮의 봄」연구

최희연 연세대학교 국어국문학과 1986 연세어문학 Vol.19 No.-

마른명태 식해의 향기성분과 기능성

최청,구태호,장운빈,최희진,우희섭,손규목 한국식생활문화학회 2002 韓國食生活文化學會誌 Vol.17 No.5

The volatile compounds of Myungtae (Alaska pollack) sikhae obtained by simultaneous steam distillation and extraction(SDE) apparatus were separated by gas chromatography(GC) and gas chromatography mass spectrometry(GC/MS). The totals of 155 volatile flavor components was identified in traditional Kyungsangdo Myungtae (Alaska pollack) sikhae, respectively. α-Zingihirene(11.03%) (E)-di-2-propenyl disulfide(7.95%) β-cironellol(6.02%), methyl allyl disulfide(3.58%), cryptone(3.39%), camphene(3.23%), pentano(3.21%), penadecanal(2.66%) and β-phellandrene(2.06%) were contained as the main compounds of Myungatae shikae. The fraction obtained from sikhae were tested for electron donating ability, angiotensin converting enzyme and xanthine oxidase inhibitory activity. There was no electron donation abilities(SC_50) of hexane and water fraction. On the other hand, the abilities of ethylacetate fraction and butanol fraction showed 310.64 ㎍.mL, 1096.49 ㎍/mL, respectively. Angiotensin converting enzyme inhibitory activities(IC_50) of ethylacetate fraction and butanol fraction were 1.623 mg/mL, 1.303 mg/mL, respectively. Xanthine oxidase inhibitory activities(IC_50) of ethylacetate fraction and butanol fraction were 3.591 mg/mL, 2.803 mg/mL, respectively.

小兒期 精神分裂症 : 思春期 以前 發病群과 思春期 以後 發病群의 臨床的 比較 硏究 A Comparison between the Two Groups with the onset prior to Puberty and after Puber

崔珍淑,鄭嬉然,崔貞美,曺洙哲,洪剛義 서울醫大 1991 精神醫學 Vol.16 No.4

Furosemide가 생쥐 신장의 Alkaline Phosphatase와 Lactate Dehydrogenase의 활성에 미치는 영향

최성협,최희석 인제대학교 1987 仁濟醫學 Vol.8 No.2

Furosemide가 생쥐 신장의 재흡수에 관여하는 alkaline phosphatase와 glucose 신생합성이나 초기 병리학적인 소견을 지시하는 세포질내 효소인 lactate dehydrogenase 활성에 미치는 영향을 조직 화학적으로 효소의 소재에 따른 변화를 규명하고자 하였다. Alkaline phosphatase 활성은 주로 근위세뇨관 상피 세포의 쇄자연에서 일어나 투여 4시간군에서 부터 저하되기 시작했으며 latctate dehydrogenase 활성변화는 근위세뇨관의 상피 세포질내에서 일어나 투여 12시간군부터 저하되기 시작했다. This experiment was carried out to investigate the effect of furosemide upon renal enzymes in the mous. Furosemide 0.1 mg was injected intraperitoneally twice a day for four days. Histochemical studies were observed on the activities of alkaline phosphatase and lactate dehydrogellase in mouse kidney. The following results were obtained: 1.The activity of alkaline phosphatase befall to decrease from th 4 hour group in the brush border of the proximal tubules. 2.The activity of lactate dehydrogenase began to decrease from the 12 hour group in the proximal tubules.

WHO 침구임상 지침서 개정을 위한 국제회의 보고

박히준,서정철,김세현,김상우,안경애,임사비나,김용석,최도영,강성길 EAST-WEST MEDICAL RESEARCH INSTITUTE KYUNG HEE UNI 2005 東西醫學硏究所 論文集 Vol.2005 No.-

The Working Group on the revision of Clinical Research Methodology for Acupuncture met in Seoul, Republic of Korea from 24 to 26 August 2005. The main objectives of the meeting were to review the regional publication guidelines for Clinical Research on Acupuncture, to make necessary revisions in the guidelines, to make recommendations on further collaboration and activities in the field of research on acupuncture, and to discuss scientific evidence-based approaches in the clinical research on acupuncture. Fourteen members from the eight Member States, one secretariat staff from the WHO Regional Office for the Western Pacific and ten observers from the Republic of Korea attended the meeting. The members presented their papers to review the current status of clinical research on acupuncture. The drafts of proposed revision to the previous guidelines for clinical research methodology on acupuncture were discussed extensively. The issues covered during the discussion included: the definition of the new terms; reorganization of clinical research design; revision and update of contents (e.g., control group); introduction of Institutional Review Board (IRB) on the section of ethical approval; additional outcomes including health-related quality of life (HRQOL), qualitative measures, etc. In the course of these discussions, the working group developed the revised guidelines for clinical research on acupuncture and made recommendations for promoting the dissemination of the revised guidelines.

공간벡터 개념을 이용한 유도 전동기 기동 토크 맥동의 저감에 관한 연구

전희종,정을기,이석태,최우진 崇實大學校 生産技術硏究所 1994 論文集 Vol.24 No.-

In this investigation the PFM(Pulse Frequency Modulation) will be used for optimizing PWM inverter pulse patterns. In traditional the pulse frequency of PWM is kept constant. But modulated PWM's frequency in this study, the sinusoidal inverter's performance should be improved. The PWM pulse patterns are definitely controlled so that the time-integral function of the voltage vectors in the space vector notation may show a circular locus. Further, performance index will be minimized because of minimizing distortion of output current. Finally, these theories are realized using single-chip microprocessor.

HBsAg 양성혈청에서의 HBeAg 및 anti-HBe 양성율과 ALT와의 관계 고찰

문희주,윤기은,박정오,배형준,최범열 서울보건대학 1996 서울보건대학 부설 한국보건과학연구소 논문집 Vol.3 No.1

The authors investigated HBeAg and anti-HBe in 1,000 cases(male 941, female 59) of HBsAg positive among the 225,512 blood donors in Seoul Nambu Blood Center in 1994. HBeAg and anti-HBe was detected by the method Enzyme Immunoassy. The results obtained were as follows ; 1. HBeAg and anti-HBe positive rates were detected 498 cases(49.8%) and 445 cases(44.5%) respectively among 1,000 cases HBsAg positive blood donors. 2. In HBsAg positive carriers, HBeAg positive rates were not significantly different between anti-HBe positive rates, but in sex were significantly different between HBeAg positive rates and anti-HBe positive rates. 3. HBeAg positive 498 cases were analyzed with age and it was found percentages of positive rates were 63.6% in 1st decade, 53.7% in 2nd decade, 20.6% in 3rd decade, 19.6% in 4th decade and 8.3% in over 5th decade, but anti-HBe positive 447 cases were 32.4% in 1st decade, 40.5% in 2nd decade, 69.2% in 3rd decade, 78.3% in 4th decade and 91. 7% in above 5th decade. 4. HBeAg positive rate decreased stepwise with age, while anti-HBe positive rate increased stepwise with age. 5. ALT abnormal cases in the HBeAg positive donors were higher than normal cases, but ALT normal cases in the anit-HBe positive donors were higher than abnormal cases.